scholarly journals Differential regulation of mitochondrial complex I and oxidative stress based on metastatic potential of colorectal cancer cells

2020 ◽  
Vol 20 (6) ◽  
pp. 1-1
Author(s):  
Neeraj Rai ◽  
Shashank Mathur ◽  
Suraj Singh ◽  
Meenakshi Tiwari ◽  
Vijay Singh ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Colorectal Cancer ◽  
Cancer Cells ◽  
Complex I ◽  
Metastatic Potential ◽  
Differential Regulation ◽  
Mitochondrial Complex I ◽  
Mitochondrial Complex ◽  
Colorectal Cancer Cells ◽  
And Oxidative Stress

Rabies virus phosphoprotein interacts with mitochondrial Complex I and induces mitochondrial dysfunction and oxidative stress

2015 ◽  
Vol 21 (4) ◽  
pp. 370-382 ◽  
Author(s):  
Wafa Kammouni ◽  
Heidi Wood ◽  
Ali Saleh ◽  
Camila M. Appolinario ◽  
Paul Fernyhough ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Mitochondrial Dysfunction ◽  
Rabies Virus ◽  
Complex I ◽  
Mitochondrial Complex I ◽  
Mitochondrial Complex ◽  
And Oxidative Stress

scholarly journals PLAC1 Enhances Metastatic Potential and is Associated with PI3K/AKT/NF-κB Signaling Pathway in Colon Cancer

2019 ◽  
Author(s):  
JIachi Ma ◽  
Shoukai Zhang ◽  
Danru Liang ◽  
Lei Li ◽  
Jun Du ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Endothelial Cells ◽  
Cancer Cells ◽  
Umbilical Vein ◽  
Metastatic Potential ◽  
Dependent Manner ◽  
Human Colorectal Cancer ◽  
Colorectal Cancer Cells ◽  
Metastatic Process ◽  
Ht 29

Abstract Background: To better explore the underlying mechanism of liver metastatic formation by placenta-specific protein 1 (PLAC1) in human colorectal cancer, we investigated the proliferation, invasion and angiogenic capabilities of human colorectal cancer cell lines with different liver metastatic potentials as well as the mechanism of action of PLAC1 in the metastatic process. Methods: The expression of PLAC1 was detected by reverse transcriptase PCR, western blot and real-time PCR. The effect of PLAC1 on metastatic potential was determined by proliferation, invasion, and angiogenesis assays, including an in vitro coculture system consisting of cancer cells and vascular endothelial cells that were used to detect the relationship between cancer cells and angiogenesis. In addition, we also determined PLAC1 downstream targets that preferentially contribute to the metastatic process. Results: PLAC1 was expressed in HT-29, WiDr and CaCo-2 colorectal cancer cells but not in Colo320 colorectal cancer cells. PLAC1 could not only significantly enhance the proliferation of CoLo320 and human umbilical vein endothelial cells (HUVECs) but could also promote the invasion of CoLo320 cells. The angiogenesis of HUVECs was enhanced by PLAC1 in a dose-dependent manner. In cocultured systems, angiogenesis was significantly increased by coculture with HT-29 cells. In addition, PLAC1 could promote angiogenesis in coculture with HT-29 cells. Furthermore, PLAC1-enhanced metastatic potential of colorectal cancer cells was dependent on activation of the PI3K/Akt/NF-κB pathway. Conclusions: The activation of PI3K/Akt/NF-κB signaling by PLAC1 may be critical for the metastasis of colorectal cancer cells. According to our results, we suggest that modification of PLAC1 function might be a promising new therapeutic approach to inhibit the aggressive spread of colorectal cancer.

Abstract 5279: LPS-TLR4 binding increases metastatic potential of colorectal cancer cells

2010 ◽  
Author(s):  
Rich YC Hsu ◽  
Carlos HF Chan ◽  
Jonathan D. Spicer ◽  
Mathieu C. Rousseau ◽  
Rushika Perera ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cancer Cells ◽  
Metastatic Potential ◽  
Colorectal Cancer Cells

scholarly journals Mitochondrial Complex I Inhibitors and Forced Oxidative Phosphorylation Synergize in Inducing Cancer Cell Death

2013 ◽  
Vol 2013 ◽  
pp. 1-14 ◽  
Author(s):  
Roberta Palorini ◽  
Tiziana Simonetto ◽  
Claudia Cirulli ◽  
Ferdinando Chiaradonna
Keyword(s):  
Cell Death ◽  
Oxidative Phosphorylation ◽  
Cancer Cells ◽  
Cancer Cell ◽  
Complex I ◽  
Mitochondrial Complex I ◽  
Low Doses ◽  
Mitochondrial Complex ◽  
Cancer Cell Death ◽  
Glucose Depletion

Cancer cells generally rely mostly on glycolysis rather than oxidative phosphorylation (OXPHOS) for ATP production. In fact, they are particularly sensitive to glycolysis inhibition and glucose depletion. On the other hand mitochondrial dysfunctions, involved in the onset of the Warburg effect, are sometimes also associated with the resistance to apoptosis that characterizes cancer cells. Therefore, combined treatments targeting both glycolysis and mitochondria function, exploiting peculiar tumor features, might be lethal for cancer cells. In this study, we show that glucose deprivation and mitochondrial Complex I inhibitors synergize in inducing cancer cell death. In particular, our results reveal that low doses of Complex I inhibitors, ineffective on immortalized cells and in high glucose growth, become specifically cytotoxic on cancer cells deprived of glucose. Importantly, the cytotoxic effect of the inhibitors on cancer cells is strongly enhanced by forskolin, a PKA pathway activator, that we have previously shown to stimulate OXPHOS. Taken together, we demonstrate that induction in cancer cells of a switch from a glycolytic to a more respirative metabolism, obtained by glucose depletion or mitochondrial activity stimulation, strongly increases their sensitivity to low doses of mitochondrial Complex I inhibitors. Our findings might be a valuable approach to eradicate cancer cells.

Oxidative stress causes epigenetic alteration of CDX1 expression in colorectal cancer cells

Gene ◽  
2013 ◽  
Vol 524 (2) ◽  
pp. 214-219 ◽  
Author(s):  
Rui Zhang ◽  
Kyoung Ah Kang ◽  
Ki Cheon Kim ◽  
Soo-Young Na ◽  
Weon Young Chang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Colorectal Cancer ◽  
Cancer Cells ◽  
Epigenetic Alteration ◽  
Colorectal Cancer Cells

scholarly journals Zerumbone increases oxidative stress in a thiol‐dependent ROS ‐independent manner to increase DNA damage and sensitize colorectal cancer cells to radiation

2014 ◽  
Vol 4 (2) ◽  
pp. 278-292 ◽  
Author(s):  
Amit Deorukhkar ◽  
Niharika Ahuja ◽  
Armando‐Lopez Mercado ◽  
Parmeswaran Diagaradjane ◽  
Uma Raju ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Colorectal Cancer ◽  
Dna Damage ◽  
Cancer Cells ◽  
Independent Manner ◽  
Colorectal Cancer Cells
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