scholarly journals Comprehensive circular RNA profiling reveals the regulatory role of circ_100242/miR-145 pathway in bladder cancer

2020 ◽  
Author(s):  
Zhaowei Zhu ◽  
Fujiang Chang ◽  
Junxiao Liu ◽  
Jiange Wang ◽  
Xuepei Zhang
Keyword(s):  
Bladder Cancer ◽  
Circular Rna ◽  
Regulatory Role ◽  
Rna Profiling

scholarly journals Dual regulatory role of CCNA2 in modulating CDK6 and MET‐mediated cell‐cycle pathway and EMT progression is blocked by miR‐381‐3p in bladder cancer

2018 ◽  
Vol 33 (1) ◽  
pp. 1374-1388 ◽  
Author(s):  
Jiangfeng Li ◽  
Yufan Ying ◽  
Haiyun Xie ◽  
Ke Jin ◽  
Huaqing Yan ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Bladder Cancer ◽  
Regulatory Role ◽  
Cell Cycle Pathway

scholarly journals Circular RNA profiling reveals a potential role of hsa_circ_IPCEF1 in papillary thyroid carcinoma

2021 ◽  
Vol 24 (2) ◽  
Author(s):  
Min Guo ◽  
Yushuang Sun ◽  
Junzhu Ding ◽  
Yong Li ◽  
Sihan Yang ◽  
...  
Keyword(s):  
Papillary Thyroid Carcinoma ◽  
Thyroid Carcinoma ◽  
Potential Role ◽  
Circular Rna ◽  
Papillary Thyroid ◽  
Rna Profiling

scholarly journals Comprehensive Circular RNA Profiling Reveals the Regulatory Role of the CircRNA-0067835/miR-155 Pathway in Temporal Lobe Epilepsy

2018 ◽  
Vol 51 (3) ◽  
pp. 1399-1409 ◽  
Author(s):  
Guo-Hua Gong ◽  
Feng-Mao An ◽  
Yu Wang ◽  
Ming Bian ◽  
Di Wang ◽  
...  
Keyword(s):  
Temporal Lobe Epilepsy ◽  
Temporal Lobe ◽  
Circular Rna ◽  
Fine Tuning ◽  
Luciferase Reporter ◽  
Circular Rnas ◽  
G1 Arrest ◽  
Rna Profiling

Background/Aims: Temporal lobe epilepsy (TLE) is the most common form of adult localization-related epilepsy that is accompanied by progressive etiopathology and high incidences of drug resistance. Circular RNAs (circRNAs) play important roles in fine-tuning gene expression, however, the expression profile and clinical significance of circRNAs in TLE remains unknown. Methods: Circular RNA microarray was conducted to identify TLE-related circRNAs. CCK8 assays and flow cytometric assays were conducted to clarify the role of circRNA in TLE in vitro. Bioinformatics analysis and in vitro experiments were conducted to clarify the mechanism of circRNA-mediated gene regulation in TLE cell. Results: 586 differentially expressed circRNAs were identified between TLE and the control tissues. The expression of circRNA-0067835 was significantly down-regulated in tissues and plasma from TLE patients. Lower circRNA-0067835 correlated to increased seizure frequency, HS, and higher Engel’s score. Overexpression of circRNA-0067835 observably decreased SH-SY5Y cell proliferation by causing G1 arrest and promoting apoptosis. Bioinformatics online programs predicted that circRNA-0067835 acted as miR-155 sponge to regulate FOXO3a expression, which was validated using luciferase reporter assay. Conclusion: Our experiments showed that circRNA-0067835 regulated refractory epilepsy progression by acting as a sponge of miR-155 to promote FOXO3a expression, indicating that circRNA-0067835 may serve as a potential therapeutic target for patients with TLE.

scholarly journals CircAPP Competes with APP mRNA to Promote Malignant Progression of Bladder Cancer

2021 ◽  
Author(s):  
Minjun Qi ◽  
Wei Cao ◽  
Xingyu Wu ◽  
Weijiang Mao ◽  
Kai Cao ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Prognostic Biomarker ◽  
Circular Rna ◽  
High Recurrence Rate ◽  
Transwell Assay ◽  
Common Cancer ◽  
Public Data

Abstract Background: Bladder cancer (BCa) is the most common cancer in the urinary system with high recurrence rate and poor prognosis. Circular RNA (circRNA) is a novel subclass of noncoding-RNAwhich participate in progression of BCa. Here, we identified a novel circRNA—circAPP and aimed to investigate the role of circAPP in progression of BCa.Method: Public data of RNA sequencing was used to identify significant circRNA related to BCa. The role of circRNAs in progression in BCa was assessed in cytotoxicity assay, transwell assay andflow cytometry. Biotin-coupled RNA pull-down and fluorescence in situ hybridization (FISH) were performed to evaluate the interaction between circRNAs and miRNAs. Results: The expression of circAPP was higher in BCa tissues and cells than in normal samples. In vitro experiments showed that knockdown of circAPP inhibited cell proliferation and impeded the metastasis of BCa cells. Mechanistically, we demonstrated that circAPP acts as a sponge for miR-186-5p and promotes host gene APP’s expression. Clinically, circAPP predicts worse overall survival of BCa patients, indicating its prognostic value.Conclusion: Our study identified that circAPP modulates metastasis of BCa through miR-186-5p/APP aixs and may serve as a promising prognostic biomarker for BCa, which provides novel insights into treatment of BCa.

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