scholarly journals CDK5RAP1 targeting NF-κB signaling pathway in human malignant melanoma A375 cell apoptosis

2018 ◽  
Author(s):  
Jikui Xiong ◽  
Yan Wang ◽  
Yanli Gu ◽  
Yadong Xue ◽  
Lin Dang ◽  
...  
Keyword(s):  
Malignant Melanoma ◽  
Signaling Pathway ◽  
Cell Apoptosis ◽  
Human Malignant Melanoma ◽  
A375 Cell ◽  
Melanoma A375 Cell

scholarly journals Pharmacological and biochemical characterization of adenosine receptors in the human malignant melanoma A375 cell line

2001 ◽  
Vol 134 (6) ◽  
pp. 1215-1226 ◽  
Author(s):  
Stefania Merighi ◽  
Katia Varani ◽  
Stefania Gessi ◽  
Elena Cattabriga ◽  
Valeria Iannotta ◽  
...  
Keyword(s):  
Malignant Melanoma ◽  
Cell Line ◽  
Adenosine Receptors ◽  
Biochemical Characterization ◽  
Human Malignant Melanoma ◽  
A375 Cell ◽  
Melanoma A375 Cell ◽  
A375 Cell Line

scholarly journals The Inhibition of Cell Growth Through the EGFR/ERK/MMP-2 Pathway Induced by Ampelopsin in the Human Malignant Melanoma A375 Cell Line

2020 ◽  
Vol 15 (3) ◽  
pp. 1934578X2091286
Author(s):  
Fan Yang ◽  
Yin-hui Yang ◽  
Wei-hui Zeng
Keyword(s):  
Cell Growth ◽  
Malignant Melanoma ◽  
Cell Line ◽  
Cyclin B1 ◽  
Matrix Metalloproteinase 2 ◽  
Dependent Manner ◽  
Human Malignant Melanoma ◽  
A375 Cell ◽  
Melanoma A375 Cell ◽  
A375 Cell Line

Malignant melanoma is one of the most aggressive skin cancers, having a very high mortality rate. However, its effective treatment is not clear. Ampelopsin, a plant flavonoid, has been reported to inhibit cell growth and/or induce apoptosis in various types of tumor. In this study, it was shown that ampelopsin significantly inhibits melanoma A375 cell line proliferation in a concentration-dependent/time-dependent manner. The flow cytometric data clearly demonstrated that ampelopsin causes cell cycle arrest in the G2/M phase. Moreover, it also confirmed that growth inhibition mediated by treatment with ampelopsin is related to the decreased expression of Cdc2, Cdc25c, cyclin B1, and activation of caspase-3 and Bax, purportedly by epidermal growth factor receptor (EGFR), extracellular regulated protein kinases, and matrix metalloproteinase-2 (MMP-2) downregulation. As a result of this work, these findings suggest that ampelopsin inhibits human malignant melanoma A375 cell line proliferation by suppressing the EGFR/ERK/MMP-2 pathway.

scholarly journals Vitamin�C induces human melanoma A375 cell apoptosis via Bax‑ and Bcl‑2‑mediated mitochondrial pathways

2019 ◽  
Author(s):  
Xiao‑Yu Chen ◽  
Ying Chen ◽  
Chuan‑Jun Qu ◽  
Zhao‑Hai Pan ◽  
Yao Qin ◽  
...  
Keyword(s):  
Vitamin C ◽  
Cell Apoptosis ◽  
Human Melanoma ◽  
A375 Cell ◽  
Melanoma A375 Cell

scholarly journals Ailanthone Induces Cell Cycle Arrest and Apoptosis in Melanoma B16 and A375 Cells

Biomolecules ◽  
2019 ◽  
Vol 9 (7) ◽  
pp. 275 ◽  
Author(s):  
Wenjing Liu ◽  
Xiaona Liu ◽  
Zhaohai Pan ◽  
Dan Wang ◽  
Minjing Li ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Malignant Melanoma ◽  
Cell Cycle Arrest ◽  
Signaling Pathway ◽  
Cell Apoptosis ◽  
Akt Signaling ◽  
Akt Signaling Pathway ◽  
Melanoma B16 ◽  
Cycle Arrest ◽  
A375 Cells

Malignant melanoma is the most lethal type of skin cancer. Previous studies have shown that ailanthone has potent antitumor activity in a variety of cell lines. However, the anti-tumor effect of ailanthone on malignant melanoma remains unclear. To investigate the anti-tumor mechanisms of ailanthone in human melanoma B16 and mouse melanoma A375 cells, the cell counting kit-8 assay, colony formation assay, DNA content analysis, Hoechst 33258, and Annexin V-FITC/PI staining were used to assess cell proliferation, cell cycle distribution, and cell apoptosis, respectively. Western blotting was performed to evaluate the expression of cell cycle- and apoptosis-related proteins and regulatory molecules. The results showed that ailanthone significantly inhibited melanoma B16 and A375 cell proliferation as well as remarkably induced cell cycle arrest at the G0–G1 phase in B16 cells and the G2–M phase in A375 cells in a dose-dependent manner. Further investigation revealed that ailanthone promoted the expression of p21 and suppressed the expression of cyclin E in B16 cells or cyclin B in A375 cells through the PI3K-Akt signaling pathway. In addition, ailanthone induced B16 and A375 cell apoptosis via a caspase-dependent mechanism. Further studies showed that ailanthone remarkably downregulated Bcl-2 and upregulated Apaf-1 and Bax, and subsequently increased mitochondrial membrane permeabilization and released cytochrome c from the mitochondria in B16 cells and A375 cells. Taken together, ailanthone induces cell cycle arrest via the PI3K-Akt signaling pathway as well as cell apoptosis via the mitochondria-mediated apoptotic signaling pathway. Ailanthone may be potentially utilized as an anti-tumor agent in the management of malignant melanoma.

Role of Bm30kc6 gene in cell apoptosis and the silk gland degradation signaling pathway in Bombyx mori L.

2020 ◽  
Vol 105 (3) ◽  
Author(s):  
Ying Xiao ◽  
Lei‐lei Li ◽  
Asma Bibi ◽  
Ning Zhang ◽  
Ting Chen ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Signaling Pathway ◽  
Cell Apoptosis ◽  
Silk Gland ◽  
Bombyx Mori L
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