Role of Bm30kc6 gene in cell apoptosis and the silk gland degradation signaling pathway in Bombyx mori L.

2020 ◽  
Vol 105 (3) ◽  
Author(s):  
Ying Xiao ◽  
Lei‐lei Li ◽  
Asma Bibi ◽  
Ning Zhang ◽  
Ting Chen ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Signaling Pathway ◽  
Cell Apoptosis ◽  
Silk Gland ◽  
Bombyx Mori L

scholarly journals Immunoregulatory Role of MicroRNA-21 in Macrophages in Response to Bacillus Calmette-Guerin Infection Involves Modulation of the TLR4/MyD88 Signaling Pathway

2017 ◽  
Vol 42 (1) ◽  
pp. 91-102 ◽  
Author(s):  
Xin Xue ◽  
Yi Qiu ◽  
Hong-Li Yang
Keyword(s):  
Cell Viability ◽  
Signaling Pathway ◽  
Cell Apoptosis ◽  
Inflammatory Factors ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Bacillus Calmette Guerin ◽  
Myd88 Signaling ◽  
Apoptosis And Necrosis

Background/Aims: The purpose of this study is to explore the immunoregulatory role of microRNA-21 (miR-21) targeting of the TLR4/MyD88 signaling pathway in macrophages in response to Bacillus Calmette-Guerin (BCG) infection. Methods: After infection with BCG, mouse RAW246.7 cells were assigned into control, BCG, miR-21 mimic + BCG, mimic-negative control (NC) + BCG, miR-21 inhibitor + BCG, inhibitor-NC + BCG, BCG + TAK242 (an inhibitor of the TLR4 signaling pathway), and miR-21 inhibitor + TAK242 + BCG groups. Western blotting and qRT-PCR were used to detect the expression of miR-21, TLR4 and MyD88. The levels of TNF-a, IL-6 and IL-10 were detected by enzyme-linked immunosorbent assay (ELISA). Cell viability was measured using an MTT assay. Cell apoptosis and necrosis rates were detected using flow cytometry. Results: Compared with the control group, miR-21 expression and levels of TNF-a, IL-6 and IL-10, as well as cell apoptosis and necrosis rates, were elevated, while expression of TLR4 and MyD88, as well as cell viability, were reduced in BCG infection groups. Compared with the BCG group, miR-21 expression was increased in the miR-21 mimic + BCG group but decreased in the miR-21 inhibitor + BCG and miR-21 inhibitor + TAK242 + BCG groups. The expression of TLR4 and MyD88, as well as the cell viability, were decreased, while levels of TNF-a, IL-6 and IL-10, as well as cell apoptosis and necrosis rates, were increased in the miR-21 mimic + BCG and TAK242 + BCG groups. The opposite trends were found in the miR-21 inhibitor + BCG group. Compared with the TAK242 + BCG group, the miR-21 inhibitor + TAK242 + BCG group had higher expression of TLR4 and MyD88 as well as higher cell viability and lower levels of TNF-a, IL-6, IL-10, cell apoptosis and necrosis rates. However, the miR-21 inhibitor + TAK242 + BCG group exhibited the opposite trends when compared with the miR-21 inhibitor + BCG group. Conclusion: Our results suggest that miR-21 can negatively modulate the TLR4/MyD88 signaling pathway, resulting in decreased cell viability, increased cell apoptosis and increased levels of inflammatory factors following BCG infection in macrophages.

scholarly journals Changes in the total protein, carbohydtate and lipid contents in selected tissues of silkworm, Bombyx mori L. under the influence of a juvenoid R394

2017 ◽  
Vol 16 ◽  
pp. 27
Author(s):  
K. Sashindran Nair ◽  
Jula S. Nair ◽  
V.A. Vijayan
Keyword(s):  
Protein Content ◽  
Bombyx Mori ◽  
Total Protein ◽  
Fat Body ◽  
Silk Gland ◽  
Total Carbohydrate ◽  
Primary Metabolites ◽  
Lipid Contents ◽  
Bombyx Mori L ◽  
Silkworm Bombyx Mori

A juvenoid. R394 (Ethyl 9-cyclohexyl-3,7-dimethyl-2,4-nonadienoate) was applied topically to 5th instar silkworm, Bombyx mori L. larvae (Hybrid: KA x NB4D2) at a dose of 0.039 nl/larva at 24, 48, 72 and 96 h, for silk yield improvement. Three major selected tissues viz., posterior silk gland (PSG), haemolymph and fat body were collected from fully-grown larvae and the total protein, total carbohydrate and total lipid contents were estimated following standard procedures. The result indicated that the content of these primary metabolites varied significantly in the selected tissues depending on the time of juvenoid application. The highest protein content was observed in the haemolymph and silk gland in the larvae treated at 72 h whereas the fat body protein content was lowest for the same treatment. The total carbohydrate was recorded lowest in the 72 h treated larvae as against the highest in the control both in haemolymph and fat body with no significant change in PSG. The total lipid content did not show any notable variation in the concentration on juvenoid administration except in the silk gland treated up to 72 h which showed a decline. The results indicate that the juvenoid induces tissue-specific responses in terms of turnover in primary metabolites which commensurates with the corresponding changes observed in the cocoon weight and cocoon shell weight.

Apoptosis of posterior silk gland of Bombyx mori during spinning period and the role of PI3K/Akt pathway

2018 ◽  
Vol 98 (1) ◽  
pp. e21450 ◽  
Author(s):  
Jia-Huan Hu ◽  
Xiao-Yu Cheng ◽  
Jin-Xin Li ◽  
Bin Xue ◽  
Jiang-Hai Tian ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Silk Gland ◽  
Akt Pathway ◽  
Posterior Silk Gland

scholarly journals Role of BmDredd during Apoptosis of Silk Gland in Silkworm, Bombyx mori

PLoS ONE ◽  
2017 ◽  
Vol 12 (1) ◽  
pp. e0169404 ◽  
Author(s):  
Rui-ting Chen ◽  
Peng Jiao ◽  
Zhen Liu ◽  
Yan Lu ◽  
Hu-hu Xin ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Silk Gland ◽  
Silkworm Bombyx Mori

scholarly journals Cell apoptosis and proliferation in rat brains after intracerebral hemorrhage: role of Wnt/β-catenin signaling pathway

2014 ◽  
Vol 44 ◽  
pp. 920-927 ◽  
Author(s):  
Ling ZHOU ◽  
Li DENG ◽  
Neng Bin CHANG ◽  
Ling DOU ◽  
Chao Xian YANG
Keyword(s):  
Intracerebral Hemorrhage ◽  
Signaling Pathway ◽  
Cell Apoptosis

scholarly journals Haemocytes during Different Stages of Lifecycle in Bombyx mori (L.)

2021 ◽  
Vol 9 (4) ◽  
pp. 43-49
Author(s):  
Babila, J. Jasmine ◽  
Keyword(s):  
Bombyx Mori ◽  
Cellular Responses ◽  
Granular Cells ◽  
Larval Stages ◽  
Before And After ◽  
Bombyx Mori L

Haemocytes in insects mediates the cellular responses like phagocytosis, encapsulation and clotting which signifies the immunological functions of any insect. In the domesticated variety of silkworm B.mori five types of haemocytes have been identified in all the stages. The most abundant cell was found to be plasmatocytes followed by Granular cells. Spherule cells and Oenocytoids were found to less during IV and V instars and least or absent in all other stages. Haemocytes found be to fluctuating before and after spinning. Plasmatocytes and granular cells decreased gradually with respect to number of days in pupa. The total haemocytic count increases gradually in the silkworm larval stages and found to be maximum at the last instar and least during adult stages where the role of haemocytes is not required because they die after laying eggs.

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