scholarly journals Combined use of alcohol in conventional chemical-induced mouse liver cancer model improves the simulation of clinical characteristics of human hepatocellular carcinoma

2017 ◽  
Vol 14 (4) ◽  
pp. 4722-4728 ◽  
Author(s):  
Bo Xin ◽  
Ying Cui ◽  
Yanxia Wang ◽  
Lei Wang ◽  
Jipeng Yin ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Liver Cancer ◽  
Mouse Liver ◽  
Clinical Characteristics ◽  
Human Hepatocellular Carcinoma ◽  
Cancer Model ◽  
Combined Use

scholarly journals A new class of protein biomarkers based on subcellular distribution: application to a mouse liver cancer model

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Tatjana Sajic ◽  
Rodolfo Ciuffa ◽  
Vera Lemos ◽  
Pan Xu ◽  
Valentina Leone ◽  
...  
Keyword(s):  
Liver Cancer ◽  
Subcellular Distribution ◽  
Mouse Liver ◽  
Cancer Model ◽  
Protein Biomarkers ◽  
New Class

scholarly journals Defeating EpCAM+ liver cancer stem cells by targeting chromatin remodeling enzyme CHD4 in human hepatocellular carcinoma

2015 ◽  
Vol 63 (5) ◽  
pp. 1164-1172 ◽  
Author(s):  
Kouki Nio ◽  
Taro Yamashita ◽  
Hikari Okada ◽  
Mitsumasa Kondo ◽  
Takehiro Hayashi ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Stem Cells ◽  
Cancer Stem Cells ◽  
Liver Cancer ◽  
Chromatin Remodeling ◽  
Human Hepatocellular Carcinoma ◽  
Liver Cancer Stem Cells

scholarly journals Effects of miR-489 targeting on SOX4 gene on proliferation and apoptosis of human hepatocellular carcinoma cells

2020 ◽  
Vol 20 (3) ◽  
pp. 1292-1298
Author(s):  
Bing Wang ◽  
Wang-Xun Jin ◽  
Yun-Li Zhang ◽  
Ling Huang ◽  
Hai-Bin Ni ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Cycle ◽  
Flow Cytometry ◽  
Cell Proliferation ◽  
Liver Cancer ◽  
Hepg2 Cells ◽  
Carcinoma Cells ◽  
Human Hepatocellular Carcinoma ◽  
Hepatocellular Carcinoma Cells ◽  
Human Hepatocellular Carcinoma Cells

Background: Hepatocellular carcinoma is one of the most common malignant tumors found all over the globe. Despite advances in surgery and chemotherapy, the five-year survival rate of patients with hepatocellular carcinoma is still low. It is known that the proliferation of hepatocellular carcinoma cells is closely related to the occurrence, development and prog- nosis of hepatocellular carcinoma. The present work investigates the expression of microRNA-489 (miR-489) in human hepatocellular carcinoma cells and its effect on the biological behavior of human hepatocellular carcinoma cells. Methods: The expression of miR-489 by fluorescence quantitative PCR detection in 30 patients with hepatoblastoma of liver cancer tissues and adjacent tissues was studied. Also, the determination of hepatoblastoma in four cell lines with differ- ent metastatic potential (HR8348, HCT116, HT29 and HEPG2) and the expression of miR-489 during miR-489 simulation process was studied. MTT assay, flow cytometry and Western blot analysis were performed to know the cell proliferation to detect the changes in cell cycle, apoptosis of cells, and SOX4 gene expression respectively. Results: RT-PCR results showed that the cells compared with pre-cancerous tissue, the expression level of miR-489 in hepatocellular carcinoma tissues than in adjacent tissue significantly decreased (P<0.05), and with liver cancer cell metastasis increased (P<0.05); analogue transfection constructed miR-489 overexpressing HEPG2 cell line by microRNA. MTT results showed that miR-489 can inhibit the proliferation of HEPG2 cells, the differences were statistically significant (P<0.05); flow cytometry results showed that miR-489 mimics was transfected into HEPG2 cells at 48 hours had no significant effect on cell cycle distribution (P > 0.05); but miR-489 expression could induce apoptosis, compared with the control group, the apoptosis of miR-489 mimics was significantly increased and the difference was statistically significant (P < 0.05). Conclusion: In conclusion, miR-489 can significantly inhibit the occurrence and development of hepatocellular carcinoma cells. The mechanism may be down regulated by the expression of SOX4 and inhibit cell proliferation. Further this study showed that the tumor cells SOX4 gene as a regulatory factor target the genes of miR-489 in hepatocellular carcinoma. Keywords: Hepatocellular carcinoma; mircroRNA-489; SOX4; apoptosis.

Smad7 regulates compensatory hepatocyte proliferation in damaged mouse liver and positively relates to better clinical outcome in human hepatocellular carcinoma

2015 ◽  
Vol 128 (11) ◽  
pp. 761-774 ◽  
Author(s):  
Teng Feng ◽  
Johanna Dzieran ◽  
Xing Gu ◽  
Silke Marhenke ◽  
Arndt Vogel ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Clinical Outcome ◽  
Mouse Model ◽  
Liver Damage ◽  
Mouse Liver ◽  
Human Hepatocellular Carcinoma ◽  
Hepatocyte Proliferation ◽  
Smad7 Expression ◽  
Dependent Mechanism

In an early liver damage mouse model, Smad7 induced compensatory hepatocyte proliferation. High Smad7 expression was associated with better prognosis in human hepatocellular carcinoma, especially in distinct subgroups. A YB-1 dependent mechanism was involved in Smad7 up-regulation in HCC.

scholarly journals Analysis of Clinical Characteristics of Hepatitis B and Alcohol-Related Liver Cancer

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Yuefei Pan ◽  
Guiliang Han
Keyword(s):  
Hepatocellular Carcinoma ◽  
Liver Cancer ◽  
Hepatitis B ◽  
Clinical Characteristics ◽  
Targeted Prevention ◽  
Multiple Parameters ◽  
Treatment Measures ◽  
Treatment Analysis ◽  
The Difference ◽  
Cancer Studies

In order to analyze the clinical characteristics of hepatitis B and alcohol-related liver cancer, this paper combines the investigation and analysis methods to analyze the clinical characteristics of hepatitis B and alcohol-related liver cancer, studies them in combination with the actual situation, and studies multiple parameters with statistical methods. Different causes of liver cancer have different pathogenic mechanisms, which may make the clinical characteristics of liver cancer different. This study mainly explores the difference in clinical characteristics between hepatitis B-related hepatocellular carcinoma and alcohol-related hepatocellular carcinoma. Through comparative analysis and analysis of the clinical characteristics of hepatitis B and alcohol-related liver cancer, the study found that hepatitis B and alcohol-related liver cancer have obvious differences in their impact mechanisms. Therefore, targeted prevention and diagnosis and treatment measures can be put forward on this basis to provide a theoretical reference for subsequent clinical treatment analysis of liver cancer.

scholarly journals Thymoquinone exerts anti-tumor activities on human hepatocellular carcinoma cells: role of angiogenesis-related genes VCAN, Grb2 and EZH2

2019 ◽  
pp. 10-16
Author(s):  
Mohammed Y. Alhassani ◽  
Samir F. Zohny ◽  
Ryan A. Sheikh ◽  
Mohammed A. Hassan ◽  
Abdulaziz A. Kalantan ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Liver Cancer ◽  
Molecular Mechanisms ◽  
Target Genes ◽  
Nigella Sativa ◽  
Human Hepatocellular Carcinoma ◽  
Biologically Active ◽  
New Drugs ◽  
Pcr Analysis ◽  
Dependent Manner

Human hepatocellular carcinoma (HCC) is the most prevalent and recurrent type of primary adult liver cancer without any effective therapy. Thus, there is an increase demands for finding new drugs and treatment strategies with selective and potent effects towards HCC. Plant-derived compounds acting as anti-cancer agents can induce apoptosis through targeting several signaling pathways. Thymoquinone (TQ), the major biologically active compound of the black seed oil (Nigella sativa) has demonstrated inhibitory activities on various cancers by targeting several pathways. In the present study, we have evaluated the molecular mechanisms that underlie the anti-proliferative, anti-metastatic, and pro-apoptotic activities exerted by TQ on liver cancer cell lineHepG2, a well-documented HCC in vitro model. Cell proliferation was determined by WST-1 assay, apoptosis rate was assessed by flow cytometry using annexin-V/7AAD staining, wound healing assay to investigate the metastasis, and the expression of target genes was assessed by Real-time RT–PCR analysis. We found that TQ significantly reduced HepG2 cell viability and induced apoptosis in a dose-dependent manner. Migration of HepG2 cells was suppressed in response to TQ. Moreover, TQ decreased the expression of several angiogenesis-related genes including versican (VCAN), growth factor receptor-bound protein 2 (Grb2), and the histone methyltransferase for lysine 27 of histone 3 (EZH2). The findings suggest that TQ exerts inhibitory effects on HCC most likely through targeting key genes involved in the invasiveness and

Abstract 4173: The oncopig cancer model as a validated model for human hepatocellular carcinoma

2016 ◽  
Author(s):  
Kwame A. Darfour-Oduro ◽  
Arun K. De ◽  
Laurie A. Rund ◽  
Ron C. Gaba ◽  
Charles E. Ray ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Human Hepatocellular Carcinoma ◽  
Cancer Model
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