scholarly journals Quercetin reduces cyclin D1 activity and induces G1 phase arrest in HepG2 cells

2016 ◽  
Vol 12 (1) ◽  
pp. 516-522 ◽  
Author(s):  
JIN ZHOU ◽  
LU LI ◽  
LI FANG ◽  
HUA XIE ◽  
WENXIU YAO ◽  
...  
Keyword(s):  
Cyclin D1 ◽  
Hepg2 Cells ◽  
G1 Phase ◽  
Phase Arrest ◽  
G1 Phase Arrest

scholarly journals SIRT1 Is Downregulated in Gastric Cancer and Leads to G1-phase Arrest via NF-κB/Cyclin D1 Signaling

2013 ◽  
Vol 11 (12) ◽  
pp. 1497-1507 ◽  
Author(s):  
Qing Yang ◽  
Bo Wang ◽  
Wei Gao ◽  
Shanying Huang ◽  
Zhifang Liu ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cyclin D1 ◽  
G1 Phase ◽  
Phase Arrest ◽  
G1 Phase Arrest

scholarly journals 6-Gingerol induces cell-cycle G1-phase arrest through AKT–GSK 3β–cyclin D1 pathway in renal-cell carcinoma

2019 ◽  
Vol 85 (2) ◽  
pp. 379-390
Author(s):  
Shan Xu ◽  
Haibao Zhang ◽  
Tianjie Liu ◽  
Wenjie Yang ◽  
Wei Lv ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Renal Cell Carcinoma ◽  
Cyclin D1 ◽  
Renal Cell ◽  
Colony Formation ◽  
G1 Phase ◽  
Phase Arrest ◽  
G1 Phase Arrest ◽  
Gsk 3Β

Abstract Purpose 6-Gingerol, a major biochemical and pharmacological active ingredient of ginger, has shown anti-inflammatory and antitumor activities against various cancers. Searching for natural products with fewer side effects for developing adjunctive therapeutic options is necessary. Methods The effects of 6-gingerol on proliferation, colony formation, and cell cycle in RCC cells were detected by a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, colony formation assay, and propidium iodide (PI) staining, respectively. Western blotting, an immunofluorescence assay, and immunohistochemical staining were performed to assess the expression of relevant proteins. A subcutaneous tumor model was set up to investigate the 6-gingerol effects on tumor growth in vivo, and the pharmacokinetics of 6-gingerol in mice were detected by LC/MS assays. Results 6-Gingerol treatment exerted time- and dose-dependent inhibition of the growth and colony formation of ACHN, 786-O, and 769-P cells, leading to a concomitant induction of cell-cycle G1-phase arrest and decrease in Ki-67 expression in the cell nucleus. Western-blotting results showed that 6-gingerol reduces phosphorylation of protein kinase B (AKT) Ser 473, cyclin-dependent kinases (CDK4), and cyclin D1 and, meanwhile, increases glycogen synthase kinase (GSK 3β) protein amount. Furthermore, the efficacy of 6-gingerol was demonstrated in an in vivo murine model of 786-O. Conclusion The above results indicate that 6-gingerol can induce cell-cycle arrest and cell-growth inhibition through the AKT–GSK 3β–cyclin D1 signaling pathway in vitro and in vivo, suggesting that 6-gingerol should be useful for renal-cell carcinoma treatment.

G1 phase arrest of human smooth muscle cells by heparin, IL-4 and cAMP is linked to repression of cyclin D1 and cdk2

1997 ◽  
Vol 133 (1) ◽  
pp. 61-69 ◽  
Author(s):  
Peter K Vadiveloo ◽  
Enrico L Filonzi ◽  
Heather R Stanton ◽  
John A Hamilton
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Cyclin D1 ◽  
Muscle Cells ◽  
G1 Phase ◽  
Phase Arrest ◽  
G1 Phase Arrest

scholarly journals Cell Cycle Regulation of Hippocampal Progenitor Cells in Experimental Models of Depression and after Treatment with Fluoxetine

2021 ◽  
Vol 22 (21) ◽  
pp. 11798
Author(s):  
Patrícia Patrício ◽  
António Mateus-Pinheiro ◽  
Ana Rita Machado-Santos ◽  
Nuno Dinis Alves ◽  
Joana Sofia Correia ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Proliferation ◽  
Cyclin D1 ◽  
Progenitor Cells ◽  
Antidepressant Treatment ◽  
G1 Phase ◽  
Phase Arrest ◽  
Hippocampal Cell ◽  
G1 Phase Arrest

Changes in adult hippocampal cell proliferation and genesis have been largely implicated in depression and antidepressant action, though surprisingly, the underlying cell cycle mechanisms are largely undisclosed. Using both an in vivo unpredictable chronic mild stress (uCMS) rat model of depression and in vitro rat hippocampal-derived neurosphere culture approaches, we aimed to unravel the cell cycle mechanisms regulating hippocampal cell proliferation and genesis in depression and after antidepressant treatment. We show that the hippocampal dentate gyrus (hDG) of uCMS animals have less proliferating cells and a decreased proportion of cells in the G2/M phase, suggesting a G1 phase arrest; this is accompanied by decreased levels of cyclin D1, E, and A expression. Chronic fluoxetine treatment reversed the G1 phase arrest and promoted an up-regulation of cyclin E. In vitro, dexamethasone (DEX) decreased cell proliferation, whereas the administration of serotonin (5-HT) reversed it. DEX also induced a G1-phase arrest and decreased cyclin D1 and D2 expression levels while increasing p27. Additionally, 5-HT treatment could partly reverse the G1-phase arrest and restored cyclin D1 expression. We suggest that the anti-proliferative actions of chronic stress in the hDG result from a glucocorticoid-mediated G1-phase arrest in the progenitor cells that is partly mediated by decreased cyclin D1 expression which may be overcome by antidepressant treatment.

scholarly journals Resibufogenin Induces G1-Phase Arrest through the Proteasomal Degradation of Cyclin D1 in Human Malignant Tumor Cells

PLoS ONE ◽  
2015 ◽  
Vol 10 (6) ◽  
pp. e0129851 ◽  
Author(s):  
Masami Ichikawa ◽  
Yoshihiro Sowa ◽  
Yosuke Iizumi ◽  
Yuichi Aono ◽  
Toshiyuki Sakai
Keyword(s):  
Cyclin D1 ◽  
Tumor Cells ◽  
Malignant Tumor ◽  
Proteasomal Degradation ◽  
G1 Phase ◽  
Phase Arrest ◽  
G1 Phase Arrest

scholarly journals Tubeimoside-1 induces oxidative stress-mediated apoptosis and G0/G1 phase arrest in human prostate carcinoma cells in vitro

2016 ◽  
Vol 37 (7) ◽  
pp. 950-962 ◽  
Author(s):  
Jing-bo Yang ◽  
Muhammad Khan ◽  
Yang-yang He ◽  
Min Yao ◽  
Yong-ming Li ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Prostate Carcinoma ◽  
Carcinoma Cells ◽  
Human Prostate ◽  
G1 Phase ◽  
Phase Arrest ◽  
G1 Phase Arrest ◽  
Human Prostate Carcinoma
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