scholarly journals Microvascular density and endothelial area correlate with Ki-67 proliferative index in surgically-treated pancreatic ductal adenocarcinoma patients

2015 ◽  
Vol 10 (2) ◽  
pp. 967-971 ◽  
Author(s):  
MICHELE AMMENDOLA ◽  
ROSARIO SACCO ◽  
ILARIA MARECH ◽  
GIUSEPPE SAMMARCO ◽  
VALERIA ZUCCALÀ ◽  
...  
Keyword(s):  
Pancreatic Ductal Adenocarcinoma ◽  
Microvascular Density ◽  
Ductal Adenocarcinoma ◽  
Proliferative Index ◽  
Ki 67

AB053. P024. Ki-67 proliferative index in resectable pancreatic ductal adenocarcinoma: does it have a prognostic role?

2018 ◽  
Vol 1 (1) ◽  
pp. AB053-AB053
Author(s):  
Francesca Aleotti ◽  
Ilaria Pergolini ◽  
Stefano Crippa ◽  
Michele Pagnanelli ◽  
Giulio Belfiori ◽  
...  
Keyword(s):  
Pancreatic Ductal Adenocarcinoma ◽  
Ductal Adenocarcinoma ◽  
Prognostic Role ◽  
Proliferative Index ◽  
Ki 67

Prognostic impact of Ki-67 proliferative index in resectable pancreatic ductal adenocarcinoma

Pancreatology ◽  
2018 ◽  
Vol 18 (4) ◽  
pp. S84
Author(s):  
Ilaria Pergolini ◽  
Stefano Crippa ◽  
Michele Pagnanelli ◽  
Giulio Belfiori ◽  
Alessandro Pucci ◽  
...  
Keyword(s):  
Pancreatic Ductal Adenocarcinoma ◽  
Ductal Adenocarcinoma ◽  
Prognostic Impact ◽  
Proliferative Index ◽  
Ki 67

scholarly journals Prognostic impact of Ki‐67 proliferative index in resectable pancreatic ductal adenocarcinoma

BJS Open ◽  
2019 ◽  
Vol 3 (5) ◽  
pp. 646-655
Author(s):  
I. Pergolini ◽  
S. Crippa ◽  
M. Pagnanelli ◽  
G. Belfiori ◽  
A. Pucci ◽  
...  
Keyword(s):  
Pancreatic Ductal Adenocarcinoma ◽  
Ductal Adenocarcinoma ◽  
Prognostic Impact ◽  
Proliferative Index ◽  
Ki 67

Establishment of patient-derived tumor xenograft (PDX) model as artificially-created liver metastasis using cryopreserved pancreatic ductal adenocarcinoma.

2020 ◽  
Vol 38 (4_suppl) ◽  
pp. 729-729
Author(s):  
Kenjiro Kimura ◽  
Ryota Tanaka ◽  
Ryosuke Amano ◽  
Shoji Kubo ◽  
Hiroaki Tanaka ◽  
...  
Keyword(s):  
Liver Metastasis ◽  
Liver Transplant ◽  
Pancreatic Ductal Adenocarcinoma ◽  
Tumor Xenograft ◽  
Ductal Adenocarcinoma ◽  
Ki 67 ◽  
Nsg Mice ◽  
Patient Derived Xenograft ◽  
Pdx Model ◽  
Successful Establishment

729 Background: Translational research using patient-derived tumor xenograft (PDX) model is progressing rapidly, and is also becoming widespread in pancreatic cancer research. The purpose of this study was to establish the liver transplant PDX model as artificially-created liver metastasis with cryopreserved primary pancreatic ductal adenocarcinoma(PDAC). Methods: The primary PDAC from 10 patients were cryopreserved and transplanted into NSG mice using liver pocket method. For engraftment and similarity evaluation, H&E staining and immunohistochemical staining such as Ki-67, p53, SMAD4, and MUC1 were performed. Results: Patient-derived xenograft was succeeded in 6 cases (60%), 10 mice (33.3%). Ki-67 index of primary PDAC and the interval of cryopreservation were significantly related to successful engraftment, respectively (p = 0.003, p = 0.007). Conclusions: In this study, we succeeded in establishing a liver transplant PDX mouse model as a preclinical platform. The factors such as Ki-67 index and the interval of cryopreservation would affect the successful establishment.

scholarly journals Glutathione S-Transferase pi-1 Knockdown Reduces Pancreatic Ductal Adenocarcinoma Growth by Activating Oxidative Stress Response Pathways

Cancers ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 1501
Author(s):  
Rahul R. Singh ◽  
Jiyan Mohammad ◽  
Megan Orr ◽  
Katie M. Reindl
Keyword(s):  
Oxidative Stress ◽  
Cell Growth ◽  
Pancreatic Ductal Adenocarcinoma ◽  
Cell Cycle Progression ◽  
Map Kinases ◽  
Ductal Adenocarcinoma ◽  
Glutathione S Transferase ◽  
Pdac Cell ◽  
Ki 67

Glutathione S-transferase pi-1 (GSTP1) plays an important role in regulating oxidative stress by conjugating glutathione to electrophiles. GSTP1 is overexpressed in breast, colon, lung, and prostate tumors, where it contributes to tumor progression and drug resistance; however, the role of GSTP1 in pancreatic ductal adenocarcinoma (PDAC) is not well understood. Using shRNA, we knocked down GSTP1 expression in three different PDAC cell lines and determined the effect on cell proliferation, cell cycle progression, and reactive oxygen species (ROS) levels. Our results show GSTP1 knockdown reduces PDAC cell growth, prolongs the G0/G1 phase, and elevates ROS in PDAC cells. Furthermore, GSTP1 knockdown results in the increased phosphorylation of c-Jun N-terminal kinase (JNK) and c-Jun and the decreased phosphorylation of extracellular signal-regulated kinase (ERK), p65, the reduced expression of specificity protein 1 (Sp1), and the increased expression of apoptosis-promoting genes. The addition of the antioxidant glutathione restored cell viability and returned protein expression levels to those found in control cells. Collectively, these data support the working hypothesis that the loss of GSTP1 elevates oxidative stress, which alters mitogen-activated protein (MAP) kinases and NF-κB signaling, and induces apoptosis. In support of these in vitro data, nude mice bearing orthotopically implanted GSTP1-knockdown PDAC cells showed an impressive reduction in the size and weight of tumors compared to the controls. Additionally, we observed reduced levels of Ki-67 and increased expression of cleaved caspase-3 in GSTP1-knockdown tumors, suggesting GSTP1 knockdown impedes proliferation and upregulates apoptosis in PDAC cells. Together, these results indicate that GSTP1 plays a significant role in PDAC cell growth and provides support for the pursuit of GSTP1 inhibitors as therapeutic agents for PDAC.

scholarly journals The effect of neuroendocrine differentiation on the survival of patients diagnosed with pancreatic ductal adenocarcinoma

2020 ◽  
pp. 38-44
Author(s):  
O. I. Kit ◽  
E. M. Franciyanc ◽  
I. S. Derizhanova ◽  
N. S. Karnaukhov ◽  
M. A. Kuznetsova ◽  
...  
Keyword(s):  
Pancreatic Ductal Adenocarcinoma ◽  
Chromogranin A ◽  
Pancreatic Tumor ◽  
Prognostic Markers ◽  
Patient Survival ◽  
Immunohistochemical Study ◽  
Neuroendocrine Differentiation ◽  
Ductal Adenocarcinoma ◽  
Ki 67 ◽  
Cancer Research Institute

Purpose of the study. Determine the frequency of MiNeN among pancreatic carcinomas and analyze the survival rate of patients depending on the percentage of cells with neuroendocrine differentiation in the tumor.Materials and methods. The current study included 31 patients with a pancreatic tumor who received surgical treatment at the Rostov Cancer Research Institute. An immunohistochemical study was conducted on biomarkers of chromogranin A, synaptophysin, and ki-67 for these patients. Based on the data obtained, 4 groups for neuroendocrine differentiation were identified.Results. The direct effect of neuroendocrine differentiation on the survival of patients with histologically confirmed pancreatic ductal adenocarcinoma has been proven. Among the sample of 31 patients, neuroendocrine differentiation was revealed in 24 cases (77%), of which 3 cases of MiNeN (10.3%) were detected. It is also proven relationship between neuroendocrine and patient survival, where an increase percent of positive cells in tumors (chromogranin A or synaptophysin) means a better prognosis. Chromogranin A is a more significant predictor of survival compared to synaptophysin. The largest difference in survival was between negative expression of chromogranin A and the presence of more than 1% positive cells in the tumor.Conclusion. We supposed that it is necessary to use neuroendocrine markers (chromogranin A and synaptophysin) in the diagnosis of ductal adenocarcinomas, even without histological signs of neuroendocrine differentiation. This will allow for a larger amount of data to determine their significance as prognostic markers.

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