scholarly journals Breast cancer stem cells expressing different stem cell markers exhibit distinct biological characteristics

2016 ◽  
Vol 14 (6) ◽  
pp. 4991-4998 ◽  
Author(s):  
Jun Shao ◽  
Wei Fan ◽  
Biao Ma ◽  
Yiping Wu
Keyword(s):  
Breast Cancer ◽  
Stem Cells ◽  
Stem Cell ◽  
Cancer Stem Cells ◽  
Biological Characteristics ◽  
Stem Cell Markers ◽  
Breast Cancer Stem Cells ◽  
Cell Markers

scholarly journals Lack of correlation of stem cell markers in breast cancer stem cells

2014 ◽  
Vol 110 (8) ◽  
pp. 2063-2071 ◽  
Author(s):  
Y Liu ◽  
R Nenutil ◽  
M V Appleyard ◽  
K Murray ◽  
M Boylan ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Stem Cells ◽  
Stem Cell ◽  
Cancer Stem Cells ◽  
Stem Cell Markers ◽  
Breast Cancer Stem Cells ◽  
Cell Markers

Abstract 3872: Differentially expressed stem cell markers in breast cancer stem cells

2011 ◽  
Author(s):  
Aline RM Lobba ◽  
Maria Fernanda PAD Forni ◽  
Carolina Perozzi ◽  
Ana Claúdia O. Carreira ◽  
Leticia Labriola ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Stem Cells ◽  
Stem Cell ◽  
Cancer Stem Cells ◽  
Differentially Expressed ◽  
Stem Cell Markers ◽  
Breast Cancer Stem Cells ◽  
Cell Markers

scholarly journals Effect of CD44 knockdown on the biological characteristics of breast cancer stem cells

2020 ◽  
Author(s):  
Zi Lei ◽  
Yang-Li Hu ◽  
Qiang Feng ◽  
Li Wang ◽  
Xin-Yan Pan ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Cycle ◽  
Stem Cells ◽  
Stem Cell ◽  
Cancer Stem Cells ◽  
Cancer Stem Cell ◽  
Biological Characteristics ◽  
Breast Cancer Stem Cells ◽  
Transcript Variants

Abstract Background: CD44 is an important surface marker of breast cancer stem cells (BCSCs), but it is unclear whether it is involved in the stemness of BCSCs. This has limited the development of new therapeutic strategies for breast cancer. Previous studies have shown that many CD44 variants generated through alternative splicing are involved in the development of breast cancer, but their exact role in BCSCs remains unclear. Therefore, we analyzed the CD44 transcript variants in BCSCs derived from the MDA-MB-435 cell line, and aimed to investigate whether CD44s knockdown could affect the biological characteristics of BCSCs.Methods: CD44+/CD24- cells were isolated among the MDA-MB-435 cells by flow cytometry, and the CD44 transcript variants were detected by RT-PCR in CD44+/CD24- cells. Due to the high expression of CD44 standard splice isoform (CD44s) in CD44+/CD24- cells, CD44s knockdown was generated using small hairpin RNA (shRNA). The effects of CD44s knockdown on the biological characteristics of BCSCs was detected using cell proliferation assay, colony formation assay, cell cycle and apoptosis assay, tumor sphere formation assay, would-healing assay, and Matrigel invasion assay. Tumorigenesis of the CD44+/CD24- cells with CD44s knockdown was investigated in vivo with NOD/SCID mice. The expression of cancer stem cell stemness-related genes, such as Bcl-2, CCNE2, EGFR, MMP7, Muc1, and Myc was also detected by qPCR.Results: Our results revealed that the mRNA expression of CD44 transcript variants was heterogeneous, and CD44s is highly expressed in BCSCs. CD44s depletion inhibited the proliferation, made cell cycle stay in G0/G1 phase, promoted the apoptosis and necrosis of BCSCs, inhibited the ability of self-renewal and invasion along with the expression of cancer stem cell-related genes in BCSCs. Moreover, CD44s knockdown inhibited the tumorigenesis ability in vivo.Conclusion: Our findings revealed that CD44s is the predominant isoform expressed in BCSCs, and is an important molecule for maintaining the properties of BCSCs. Targeting CD44s in BCSCs may be a potential new direction for breast cancer treatment.

scholarly journals The effects and mechanisms of SLC34A2 on maintaining stem cell-like phenotypes in CD147+breast cancer stem cells

Tumor Biology ◽  
2017 ◽  
Vol 39 (4) ◽  
pp. 101042831769592 ◽  
Author(s):  
Yonggang Lv ◽  
Ting Wang ◽  
Jing Fan ◽  
Zhenzhen Zhang ◽  
Juliang Zhang ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Stem Cells ◽  
Stem Cell ◽  
Cancer Stem Cells ◽  
Breast Cancer Stem Cells

scholarly journals Gastric Cancer Stem Cells Survive in Stress Environments via Their Autophagy System

2020 ◽  
Author(s):  
Shingo Togano ◽  
Masakazu Yashiro ◽  
Go Masuda ◽  
Atsushi Sugimoto ◽  
Yuichiro Miki ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Stem Cells ◽  
Stem Cell ◽  
Electron Microscope ◽  
Cancer Stem Cells ◽  
Western Blot ◽  
Stem Cell Markers ◽  
Cell Markers ◽  
Autophagy Inhibitor ◽  
Stress Environments

Abstract Background: It has been reported that cancer stem cells (CSCs) play an important role in the progression of carcinoma and have a high potential for survival in various stress environments such as starvation and hypoxia. However, the mechanisms responsible for the capacity of CSCs to survive under stresses have been unclear. The aim of this study was to clarify the significance of the autophagy systems of CSCs under stress environments.Methods: Four human gastric cancer cell line, OCUM-12, OCUM-2MD3, MKN-45 and MKN-74 were used. Side population (SP) cells were sorted from the parent OCUM-12 and OCUM-2MD3, as CSC rich cells. The expression of stem cell markers was examined by RT-PCR. The viability of cancer cells under starvation and hypoxia (1%O2) was evaluated by MTT assay with or without the autophagy inhibitor, chloroquine. The expression level of the autophagy molecule LC3-II was examined by western blot. The numbers of autophagosomes and autolysosomes were counted by electron microscope.Results: SP cells of OCUM-12 showed a higher expression of stem cell markers and higher viability in starvation and hypoxia. Western blot and electron microscope examinations indicated that the autophagy was more induced in SP cells than in parent cells. The autophagy inhibitor significantly decreased the viability and the proportion of SP cells under the stress environments.Conclusions: Cancer stem cells of gastric cancer might maintain their viability under stress environments of starvation and hypoxia via the autophagy system. Autophagy inhibitors might be a promising therapeutic agent for gastric cancer.

Breast Cancer Stem Cells and Sex Steroid Hormones

2019 ◽  
Vol 14 (5) ◽  
pp. 398-404 ◽  
Author(s):  
Iván Flores-Ramírez ◽  
Noemi Baranda-Avila ◽  
Elizabeth Langley
Keyword(s):  
Breast Cancer ◽  
Stem Cells ◽  
Stem Cell ◽  
Cancer Stem Cells ◽  
Steroid Hormones ◽  
Sex Steroid Hormones ◽  
Sex Steroid ◽  
Breast Cancer Stem Cells ◽  
Tumor Initiating Cells ◽  
Intrinsic Resistance

Breast cancer stem cells (BCSCs) are a small population of tumor-initiating cells that express stem cell-associated markers. In recent years, their properties and mechanisms of regulation have become the focus of intense research due to their intrinsic resistance to conventional cancer therapies. This review describes breast cancer stem cell origin, signaling pathways involved in self-renewal, such as Wnt, Notch and Hedgehog, biomarkers linked to stemness, and the role of sex steroid hormones in BCSC regulation.

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