scholarly journals Caspase-3 may be employed as an early predictor for fracture-induced osteonecrosis of the femoral head in a canine model

2012 ◽  
Vol 6 (3) ◽  
pp. 611-614 ◽  
Author(s):  
YOU-SHUI GAO ◽  
SHANG-CHUN GUO ◽  
HAO DING ◽  
CHANG-QING ZHANG
Keyword(s):  
Femoral Head ◽  
Caspase 3 ◽  
Canine Model ◽  
Early Predictor

scholarly journals Induction of PI3K/Akt-Mediated Apoptosis in Osteoclasts Is a Key Approach for Buxue Tongluo Pills to Treat Osteonecrosis of the Femoral Head

2021 ◽  
Vol 12 ◽  
Author(s):  
Dan Wang ◽  
Yicheng Liu ◽  
Dandan Tang ◽  
Shujun Wei ◽  
Jiayi Sun ◽  
...  
Keyword(s):  
Femoral Head ◽  
Functional Annotation ◽  
Caspase 3 ◽  
Differentially Expressed Gene ◽  
Pathway Enrichment Analysis ◽  
Therapeutic Effects ◽  
Dependent Manner ◽  
Clinical Practices ◽  
Key Genes

The Buxue Tongluo pill (BTP) is a self-made pill with the functions of nourishing blood, promoting blood circulation, dredging collaterals, and relieving pain. It consists of Angelica sinensis (Oliv.) Diels, Pheretima aspergillum (E.Perrier), Panax notoginseng (Burk.) F. H. Chen, Astragalus membranaceus (Fisch.) Bge, and Glycyrrhiza uralensis Fisch. Various clinical practices have confirmed the therapeutic effect of BTP on osteonecrosis of the femoral head (ONFH), but little attention has been paid to the study of its bioactive ingredients and related mechanisms of action. In this study, UPLC/MS-MS combined with GEO data mining was used to construct a bioactive ingredient library of BTP and a differentially expressed gene (DEG) library for ONFH. Subsequently, Cytoscape (3.7.2) software was used to analyze the protein–protein interaction between BTP and DEGs of ONFH to screen the key targets, and functional annotation analysis and pathway enrichment analysis were carried out. Finally, 34 bioactive compounds were screened, which acted on 1,232 targets. A total of 178 DEGs were collected, and 17 key genes were obtained after two screenings. By bioinformatics annotation on these key genes, a total of 354 gene ontology (GO) functional annotation analyses and 42 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were obtained. The present study found that GO and KEGG enrichment were mainly related to apoptosis, suggesting that BTP may exert an anti-ONFH effect by promoting osteoclast apoptosis. Experiments in vitro demonstrated that BTP could increase the mitochondrial membrane potential (MMP) and induce remarkable apoptosis in osteoclasts. Furthermore, we determined the apoptosis marker of cleaved(C)-caspase-3, bcl-2, and bax and found that BTP could upregulate the C-caspase-3 and bax expression in osteoclasts and decrease the expression of bcl-2, p-Akt, and p-PI3K in a dose-dependent manner, indicating that BTP could induce PI3K/Akt-mediated apoptosis in osteoclasts to treat ONFH. This study explored the pharmacodynamic basis and mechanism of BTP against ONFH from the perspective of systemic pharmacology, laying a foundation for further elucidating the therapeutic effects of BTP against ONFH.

scholarly journals Protective Effects of Total Saponins of Panax Notoginseng on Steroid-Induced Avascular Necrosis of the Femoral Head In Vivo and In Vitro

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Heng-feng Yuan ◽  
Jian-feng Pan ◽  
Shuo Li ◽  
Chang-an Guo ◽  
Shu-hao Liu ◽  
...  
Keyword(s):  
Femoral Head ◽  
Protective Effect ◽  
Avascular Necrosis ◽  
Caspase 3 ◽  
In Vitro Study ◽  
Panax Notoginseng ◽  
Protective Effects ◽  
Model Group

This research was designed to investigate the protective effects of TSPN on steroid-induced avascular necrosis of the femoral head (ANFH) and the likely mechanisms of those effects. As an in vivo study, TSPN was shown to be protective against steroid-induced ANFH due to the upregulation of VEGF-A. Furthermore, TSPN attenuated the apoptosis of osteocytes and reduced the expression of Caspase-3 relative to the model group. As an in vitro study, TSPN exerted a concentration-dependent protective effect against apoptosis in MC3T3-E1 cells. Moreover, TSPN (at a dose of 100 μg/mL) significantly reversed the dexamethasone-induced augmentation of Caspase-3 expression and activity. Therefore, our study demonstrated that TSPN had a protective effect against steroid-induced ANFH that was related to the upregulation of VEGF-A and the inhibition of apoptosis and Caspase-3 activation.

STAT1-caspase 3 pathway in the apoptotic process associated with steroid-induced necrosis of the femoral head

2014 ◽  
Vol 45 (4) ◽  
pp. 473-485 ◽  
Author(s):  
Xinxian Xu ◽  
Hong Wen ◽  
Yuezheng Hu ◽  
Huachen Yu ◽  
Yu Zhang ◽  
...  
Keyword(s):  
Femoral Head ◽  
Caspase 3 ◽  
Apoptotic Process

scholarly journals A Canine Model of Femoral Head Osteonecrosis Induced by an Ethanol Injection Navigated by a Novel Template

2013 ◽  
Vol 10 (11) ◽  
pp. 1451-1458 ◽  
Author(s):  
Cairu Wang ◽  
Junlin Wang ◽  
Yongquan Zhang ◽  
Chaofan Yuan ◽  
Da Liu ◽  
...  
Keyword(s):  
Femoral Head ◽  
Canine Model ◽  
Ethanol Injection ◽  
Femoral Head Osteonecrosis

scholarly journals Injury-to-surgery interval does not affect the occurrence of osteonecrosis of the femoral head: A prospective study in a canine model of femoral neck fractures

2012 ◽  
Vol 18 (7) ◽  
pp. BR259-BR264 ◽  
Author(s):  
You-Shui Gao ◽  
Zhen-Hong Zhu ◽  
Sheng-Bao Chen ◽  
Xiang-Guo Cheng ◽  
Dong-Xu Jin ◽  
...  
Keyword(s):  
Femoral Head ◽  
Femoral Neck ◽  
Prospective Study ◽  
Canine Model ◽  
Femoral Neck Fractures ◽  
A Prospective Study

scholarly journals Magnetic Targeting of HU-MSCs in the Treatment of Glucocorticoid-Associated Osteonecrosis of the Femoral Head Through Akt/Bcl2/Bad/Caspase-3 Pathway

2020 ◽  
Vol Volume 15 ◽  
pp. 3605-3620
Author(s):  
Lian Duan ◽  
Jianlin Zuo ◽  
Fuqiang Zhang ◽  
Binxi Li ◽  
Zhonghang Xu ◽  
...  
Keyword(s):  
Femoral Head ◽  
Caspase 3 ◽  
Magnetic Targeting

scholarly journals Magnetic Targeting of HU-MSCs in the Treatment of Glucocorticoid-Associated Osteonecrosis of the Femoral Head Through Akt/Bcl2/Bad/Caspase-3 Pathway [Corrigendum]

2021 ◽  
Vol Volume 16 ◽  
pp. 6531-6533
Author(s):  
Lian Duan ◽  
Jianlin Zuo ◽  
Fuqiang Zhang ◽  
Binxi Li ◽  
Zhonghang Xu ◽  
...  
Keyword(s):  
Femoral Head ◽  
Caspase 3 ◽  
Magnetic Targeting
Sign in / Sign up

Export Citation Format

Share Document