scholarly journals Magnetic Targeting of HU-MSCs in the Treatment of Glucocorticoid-Associated Osteonecrosis of the Femoral Head Through Akt/Bcl2/Bad/Caspase-3 Pathway [Corrigendum]

2021 ◽  
Vol Volume 16 ◽  
pp. 6531-6533
Author(s):  
Lian Duan ◽  
Jianlin Zuo ◽  
Fuqiang Zhang ◽  
Binxi Li ◽  
Zhonghang Xu ◽  
...  
Keyword(s):  
Femoral Head ◽  
Caspase 3 ◽  
Magnetic Targeting

scholarly journals Magnetic Targeting of HU-MSCs in the Treatment of Glucocorticoid-Associated Osteonecrosis of the Femoral Head Through Akt/Bcl2/Bad/Caspase-3 Pathway

2020 ◽  
Vol Volume 15 ◽  
pp. 3605-3620
Author(s):  
Lian Duan ◽  
Jianlin Zuo ◽  
Fuqiang Zhang ◽  
Binxi Li ◽  
Zhonghang Xu ◽  
...  
Keyword(s):  
Femoral Head ◽  
Caspase 3 ◽  
Magnetic Targeting

scholarly journals Induction of PI3K/Akt-Mediated Apoptosis in Osteoclasts Is a Key Approach for Buxue Tongluo Pills to Treat Osteonecrosis of the Femoral Head

2021 ◽  
Vol 12 ◽  
Author(s):  
Dan Wang ◽  
Yicheng Liu ◽  
Dandan Tang ◽  
Shujun Wei ◽  
Jiayi Sun ◽  
...  
Keyword(s):  
Femoral Head ◽  
Functional Annotation ◽  
Caspase 3 ◽  
Differentially Expressed Gene ◽  
Pathway Enrichment Analysis ◽  
Therapeutic Effects ◽  
Dependent Manner ◽  
Clinical Practices ◽  
Key Genes

The Buxue Tongluo pill (BTP) is a self-made pill with the functions of nourishing blood, promoting blood circulation, dredging collaterals, and relieving pain. It consists of Angelica sinensis (Oliv.) Diels, Pheretima aspergillum (E.Perrier), Panax notoginseng (Burk.) F. H. Chen, Astragalus membranaceus (Fisch.) Bge, and Glycyrrhiza uralensis Fisch. Various clinical practices have confirmed the therapeutic effect of BTP on osteonecrosis of the femoral head (ONFH), but little attention has been paid to the study of its bioactive ingredients and related mechanisms of action. In this study, UPLC/MS-MS combined with GEO data mining was used to construct a bioactive ingredient library of BTP and a differentially expressed gene (DEG) library for ONFH. Subsequently, Cytoscape (3.7.2) software was used to analyze the protein–protein interaction between BTP and DEGs of ONFH to screen the key targets, and functional annotation analysis and pathway enrichment analysis were carried out. Finally, 34 bioactive compounds were screened, which acted on 1,232 targets. A total of 178 DEGs were collected, and 17 key genes were obtained after two screenings. By bioinformatics annotation on these key genes, a total of 354 gene ontology (GO) functional annotation analyses and 42 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were obtained. The present study found that GO and KEGG enrichment were mainly related to apoptosis, suggesting that BTP may exert an anti-ONFH effect by promoting osteoclast apoptosis. Experiments in vitro demonstrated that BTP could increase the mitochondrial membrane potential (MMP) and induce remarkable apoptosis in osteoclasts. Furthermore, we determined the apoptosis marker of cleaved(C)-caspase-3, bcl-2, and bax and found that BTP could upregulate the C-caspase-3 and bax expression in osteoclasts and decrease the expression of bcl-2, p-Akt, and p-PI3K in a dose-dependent manner, indicating that BTP could induce PI3K/Akt-mediated apoptosis in osteoclasts to treat ONFH. This study explored the pharmacodynamic basis and mechanism of BTP against ONFH from the perspective of systemic pharmacology, laying a foundation for further elucidating the therapeutic effects of BTP against ONFH.

scholarly journals P11.42 Magnetic targeting of the granzyme B functionalized nanoparticles for therapy of glioblastoma

2019 ◽  
Vol 21 (Supplement_3) ◽  
pp. iii52-iii52
Author(s):  
M Shevtsov ◽  
S Stangl ◽  
Y Marchenko ◽  
L Yakovleva ◽  
V Ryzhov ◽  
...  
Keyword(s):  
Magnetic Field ◽  
Overall Survival ◽  
Tumor Cells ◽  
Caspase 3 ◽  
Granzyme B ◽  
Single Agent ◽  
Magnetic Drug Targeting ◽  
Magnetic Targeting ◽  
Dependent Manner ◽  
Biodistribution Studies

Abstract BACKGROUND Magnetic drug targeting by employing external magnetic fields represents a promising approach for treatment of glioblastoma. To increase the anti-tumor therapeutic effect magnetic nanocarriers could be functionalized with tumor-targeting bioligands such as granzyme B (GrB). The serine protease GrB that is produced as an effector molecule by activated NK and T cells can induce specific tumor cell apoptosis. MATERIAL AND METHODS The targeting potential of the dextran-coated superparamagnetic iron oxide nanoparticles conjugated with GrB (GrB-SPIONs) was assessed in glioblastoma cells (U87, C6, GL261) by flow cytometry, confocal and electron microscopies. Magnetic field strength (320 mT) in the models of U87 glioblastoma in NMRI nu/nu mice and C6 glioma in Wistar rats was achieved by employing NdFeB cylindrical ferromagnet. The irradiation of the implanted tumors was performed using the CT-image guided Small Animal Radiation Research Platform (SARRP). Accumulation of the nanoparticles was assessed in paraffine-embedded specimens employing Prussian blues staining. Sections were additionally analyzed by IHC for apoptosis (caspase 3). RESULTS Synthesized GrB-SPIONs incorporated into the cytoplasm of tumor cells via the endolysosomal pathway and induced apoptosis in a dose-dependent manner. Intravenous injection of GrB-SPIONs resulted in the glioma-specific retention of the nanoparticles as was shown by high-field (11 T) magnetic resonance imaging and biodistribution studies (NLR-M2 measurements). Magnetic targeting of the nanoparticles in vivo drastically enhanced the accumulation of nanoparticles to the location of the magnet. Thus the local retention of nanoparticles was 12.3-fold higher as compared to application of GrB-SPIONs without external magnetic field. The presence of granzyme B on SPIONs has been shown to promote tumor cells death (IHC staining for caspase 3) and significantly increased overall survival. A combination of nanoparticle treatment with a single radiation dose (10 Gy) significantly prolonged the survival of rats as compared to animals treated only with magnetic targeting of GrB-SPIONs or radiotherapy alone (P<0.001). CONCLUSION Single-agent therapy with GrB-SPIONs demonstrated an impressive increase in overall survival of tumor-bearing animals. Combinatorial regimen employing magnetic targeting and stereotactic radiotherapy further enhanced the therapeutic potential of magnetic conjugates.

scholarly journals Protective Effects of Total Saponins of Panax Notoginseng on Steroid-Induced Avascular Necrosis of the Femoral Head In Vivo and In Vitro

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Heng-feng Yuan ◽  
Jian-feng Pan ◽  
Shuo Li ◽  
Chang-an Guo ◽  
Shu-hao Liu ◽  
...  
Keyword(s):  
Femoral Head ◽  
Protective Effect ◽  
Avascular Necrosis ◽  
Caspase 3 ◽  
In Vitro Study ◽  
Panax Notoginseng ◽  
Protective Effects ◽  
Model Group

This research was designed to investigate the protective effects of TSPN on steroid-induced avascular necrosis of the femoral head (ANFH) and the likely mechanisms of those effects. As an in vivo study, TSPN was shown to be protective against steroid-induced ANFH due to the upregulation of VEGF-A. Furthermore, TSPN attenuated the apoptosis of osteocytes and reduced the expression of Caspase-3 relative to the model group. As an in vitro study, TSPN exerted a concentration-dependent protective effect against apoptosis in MC3T3-E1 cells. Moreover, TSPN (at a dose of 100 μg/mL) significantly reversed the dexamethasone-induced augmentation of Caspase-3 expression and activity. Therefore, our study demonstrated that TSPN had a protective effect against steroid-induced ANFH that was related to the upregulation of VEGF-A and the inhibition of apoptosis and Caspase-3 activation.

scholarly journals Caspase-3 may be employed as an early predictor for fracture-induced osteonecrosis of the femoral head in a canine model

2012 ◽  
Vol 6 (3) ◽  
pp. 611-614 ◽  
Author(s):  
YOU-SHUI GAO ◽  
SHANG-CHUN GUO ◽  
HAO DING ◽  
CHANG-QING ZHANG
Keyword(s):  
Femoral Head ◽  
Caspase 3 ◽  
Canine Model ◽  
Early Predictor

STAT1-caspase 3 pathway in the apoptotic process associated with steroid-induced necrosis of the femoral head

2014 ◽  
Vol 45 (4) ◽  
pp. 473-485 ◽  
Author(s):  
Xinxian Xu ◽  
Hong Wen ◽  
Yuezheng Hu ◽  
Huachen Yu ◽  
Yu Zhang ◽  
...  
Keyword(s):  
Femoral Head ◽  
Caspase 3 ◽  
Apoptotic Process
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