scholarly journals ShRNA-mediated gene silencing of AHR promotes the differentiation of P19 mouse embryonic carcinoma cells into cardiomyocytes

2012 ◽  
Vol 6 (3) ◽  
pp. 513-518 ◽  
Author(s):  
CHUN ZHU ◽  
YU-LIN CHEN ◽  
XUE-JIE WANG ◽  
XIAO-SHAN HU ◽  
ZHANG-BIN YU ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Carcinoma Cells ◽  
Embryonic Carcinoma ◽  
Embryonic Carcinoma Cells

scholarly journals Retinoic Acid Induces Differentiation of Mouse F9 Embryonic Carcinoma Cell by Modulating the miR-485 Targeting of Abhd2

2019 ◽  
Vol 20 (9) ◽  
pp. 2071 ◽  
Author(s):  
Mengying Yu ◽  
Lei Zhang ◽  
Yingxiang Liu ◽  
Defu Liu ◽  
Zekun Guo
Keyword(s):  
Retinoic Acid ◽  
Cell Differentiation ◽  
Carcinoma Cell ◽  
Carcinoma Cells ◽  
Extracellular Signal Regulated Kinase ◽  
Embryonic Carcinoma ◽  
Extracellular Signal ◽  
Embryonic Carcinoma Cells ◽  
Embryonic Carcinoma Cell ◽  
Mediate Cell

Retinoic acid (RA) plays a key role in pluripotent cell differentiation. In F9 embryonic carcinoma cells, RA can induce differentiation towards somatic lineages via the Ras-extracellular signal-regulated kinase (Ras/Erk) pathway, but the mechanism through which it induces the Erk1/2 phosphorylation is unclear. Here, we show that miR-485 is a positive regulator that targets α/β-hydrolase domain-containing protein 2 (Abhd2), which can result in Erk1/2 phosphorylation and triggers differentiation. RA up-regulates miR-485 and concurrently down-regulates Abhd2. We verified that Abhd2 is targeted by miR-485 and they both can influence the phosphorylation of Erk1/2. In summary, RA can mediate cell differentiation by phosphorylating Erk1/2 via miR-485 and Abhd2.

Analysis of NO synthase expression in neuronal, astroglial and fibroblast-like derivatives differen-tiating from PCC7-Mzl embryonic carcinoma cells

1999 ◽  
Vol 78 (2) ◽  
pp. 134-142 ◽  
Author(s):  
Ingolf Gath ◽  
Albrecht Steppuhn ◽  
Alfred Maelicke ◽  
Sigrid Reinhardt ◽  
Ulrich Förstermann
Keyword(s):  
No Synthase ◽  
Carcinoma Cells ◽  
Embryonic Carcinoma ◽  
Embryonic Carcinoma Cells
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