scholarly journals Flow cytometric analysis of circulating endothelial cells and endothelial progenitors for clinical purposes in oncology: A critical evaluation

2016 ◽  
Vol 4 (6) ◽  
pp. 909-917 ◽  
Author(s):  
MARCO DANOVA ◽  
GIUDITTA COMOLLI ◽  
MARIANGELA MANZONI ◽  
MARTINA TORCHIO ◽  
GIULIANO MAZZINI
Keyword(s):  
Endothelial Cells ◽  
Critical Evaluation ◽  
Flow Cytometric Analysis ◽  
Circulating Endothelial Cells ◽  
Flow Cytometric ◽  
Endothelial Progenitors

scholarly journals Circulating endothelial cells transiently increase in peripheral blood after kidney transplantation

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
H. Tejeda-Mora ◽  
J. G. H. P. Verhoeven ◽  
W. Verschoor ◽  
K. Boer ◽  
D. A. Hesselink ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Kidney Transplantation ◽  
Kidney Transplant ◽  
Allograft Rejection ◽  
Flow Cytometric Analysis ◽  
Principal Component ◽  
Circulating Endothelial Cells ◽  
Clinical Markers ◽  
Kidney Transplant Recipients ◽  
Post Transplant

AbstractThe diagnosis of kidney allograft rejection is based on late histological and clinical markers. Early, specific and minimally-invasive biomarkers may improve rejection diagnosis. Endothelial cells (EC) are one of the earliest targets in kidney transplant rejection. We investigated whether circulating EC (cEC) could serve as an earlier and less invasive biomarker for allograft rejection. Blood was collected from a cohort of 51 kidney transplant recipients before and at multiple timepoints after transplantation, including during a for cause biopsy. The number and phenotype of EC was assessed by flow-cytometric analysis. Unbiased selection of EC was done using principal component (PCA) analysis. Paired analysis revealed a transient cEC increase of 2.1-fold on the third day post-transplant, recovering to preoperative levels at seventh day post-transplant and onwards. Analysis of HLA subtype demonstrated that cEC mainly originate from the recipient. cEC levels were not associated with allograft rejection, allograft function or other allograft pathologies. However, cEC in patients with allograft rejection and increased levels of cEC showed elevated levels of KIM-1 (kidney injury marker-1). These findings indicate that cEC numbers and phenotype are affected after kidney transplantation but may not improve rejection diagnosis.

Detection of Circulating Endothelial Cells: CD146-Based Magnetic Separation Enrichment or Flow Cytometric Assay?

2007 ◽  
Vol 25 (5) ◽  
pp. e1-e2 ◽  
Author(s):  
Françoise Dignat-George ◽  
Florence Sabatier ◽  
Andrew Blann ◽  
Alexander Woywodt
Keyword(s):  
Endothelial Cells ◽  
Magnetic Separation ◽  
Circulating Endothelial Cells ◽  
Flow Cytometric Assay ◽  
Flow Cytometric

Salidroside Inhibits Endogenous Hydrogen Peroxide Induced Cytotoxicity of Endothelial Cells

2013 ◽  
Vol 750-752 ◽  
pp. 1529-1532 ◽  
Author(s):  
Xing Yu Zhao ◽  
Lian Hai Jin ◽  
Dong Jun Wang ◽  
Bin Xu ◽  
Wei Zhang ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Endothelial Cells ◽  
Glucose Oxidase ◽  
Flow Cytometric Analysis ◽  
Steady States ◽  
Protective Effects ◽  
Human Endothelial Cells ◽  
Flow Cytometric ◽  
Dose Dependent ◽  
Mtt Assays

To explore the protective effects of salidroside against endogenous hydrogen peroxide (H2O2) -induced cytotoxicity in human endothelial cells (EVC-304). EVC-304 cells were incubated in the presence or absence of low steady states of H2O2 (34μM) generated by glucose oxidase (GOX) with or without salidroside. MTT assays were performed, together with flow cytometric analysis using propidium (PI) label. The results indicated that salidroside could attenuate H2O2 induced cytotoxicity in EVC-304 cells in a dose-dependent pattern. Furthermore, flow cytometric analysis revealed that salidroside could also inhibited the G2/M arrest induced by endogenous hydrogen. The present study demonstrates that salidroside could inhibit endogenous hydrogen peroxide induced cytotoxicity of endothelial cells .

scholarly journals Flow cytometric evaluation of circulating endothelial cells: A new protocol for identifying endothelial cells at several stages of differentiation

2007 ◽  
Vol 82 (8) ◽  
pp. 706-711 ◽  
Author(s):  
Hakan Ozdogu ◽  
Oktay Sozer ◽  
Can Boga ◽  
llknur Kozanoglu ◽  
Erkan Maytalman ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Circulating Endothelial Cells ◽  
Flow Cytometric ◽  
Flow Cytometric Evaluation

scholarly journals Appearance of claudin-5+ leukocyte subtypes in the blood and CNS during progression of EAE

2021 ◽  
Vol 18 (1) ◽  
Author(s):  
Dylan Krajewski ◽  
Debayon Paul ◽  
Shujun Ge ◽  
Evan Jellison ◽  
Joel S. Pachter
Keyword(s):  
T Cells ◽  
Endothelial Cells ◽  
Demyelinating Disease ◽  
Flow Cytometric Analysis ◽  
Autoimmune Encephalomyelitis ◽  
Flow Cytometric ◽  
Leukocyte Transendothelial Migration ◽  
Transient Interactions ◽  
Circulating T Cells ◽  
Experimental Autoimmune

Abstract Background Tight junctions (TJs) are membrane specializations characteristic of barrier-forming membranes, which function to seal the aqueous pathway between endothelial cells or epithelial cells and, thereby, obstruct intercellular solute and cellular movement. However, previous work from our laboratory found that claudin-5 (CLN-5), a TJ protein prominent at the blood–brain barrier (BBB), was also detected, ectopically, on leukocytes (CLN-5+) in the blood and central nervous system (CNS) of mice with experimental autoimmune encephalomyelitis (EAE), a neuroinflammatory, demyelinating disease that is a model for multiple sclerosis. CLN-5 was further shown to be transferred from endothelial cells to circulating leukocytes during disease, prompting consideration this action is coupled to leukocyte transendothelial migration (TEM) into the CNS by fostering transient interactions between corresponding leukocyte and endothelial junctional proteins at the BBB. Methods To begin clarifying the significance of CLN-5+ leukocytes, flow cytometry was used to determine their appearance in the blood and CNS during EAE. Results Flow cytometric analysis revealed CLN-5+ populations among CD4 and CD8 T cells, B cells, monocytes and neutrophils, and these appeared with varying kinetics and to different extents in both blood and CNS. CLN-5 levels on circulating T cells further correlated highly with activation state. And, the percentage of CLN-5+ cells among each of the subtypes analyzed was considerably higher in CNS tissue than in blood, consistent with the interpretation that CLN-5+ leukocytes gain preferred access to the CNS. Conclusion Several leukocyte subtypes variably acquire CLN-5 in blood before they enter the CNS, an event that may represent a novel mechanism to guide leukocytes to sites for paracellular diapedesis across the BBB.

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