scholarly journals Flow Cytometric Analysis of Phosphorylated Histone H2AX Following Exposure to Ionizing Radiation in Human Microvascular Endothelial Cells

2006 ◽  
Vol 47 (3/4) ◽  
pp. 245-257 ◽  
Author(s):  
Yasushi KATAOKA ◽  
Vytautas P. BINDOKAS ◽  
Ryan C. DUGGAN ◽  
Jeffrey S. MURLEY ◽  
David J. GRDINA
Keyword(s):  
Endothelial Cells ◽  
Ionizing Radiation ◽  
Flow Cytometric Analysis ◽  
Microvascular Endothelial Cells ◽  
Histone H2ax ◽  
Flow Cytometric ◽  
Microvascular Endothelial

scholarly journals Ionizing radiation reduces ADAM10 expression in brain microvascular endothelial cells undergoing stress-induced senescence

Aging ◽  
2017 ◽  
Vol 9 (4) ◽  
pp. 1248-1268 ◽  
Author(s):  
Lucinda S. McRobb ◽  
Matthew J. McKay ◽  
Jennifer R. Gamble ◽  
Michael Grace ◽  
Vaughan Moutrie ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Ionizing Radiation ◽  
Microvascular Endothelial Cells ◽  
Brain Microvascular Endothelial Cells ◽  
Adam10 Expression ◽  
Microvascular Endothelial

scholarly journals Ionizing radiation induces immediate protein acetylation changes in human cardiac microvascular endothelial cells

2015 ◽  
Vol 56 (4) ◽  
pp. 623-632 ◽  
Author(s):  
Zarko Barjaktarovic ◽  
Stefan J. Kempf ◽  
Arundhathi Sriharshan ◽  
Juliane Merl-Pham ◽  
Michael J. Atkinson ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Ionizing Radiation ◽  
Microvascular Endothelial Cells ◽  
Protein Acetylation ◽  
Microvascular Endothelial ◽  
Cardiac Microvascular Endothelial Cells

Resveratrol Reduces Matrix Metalloproteinase-2 Activity Induced by Oxygen-Glucose Deprivation and Reoxygenation in Human Cerebral Microvascular Endothelial Cells

2012 ◽  
Vol 82 (4) ◽  
pp. 267-274 ◽  
Author(s):  
Zahide Cavdar ◽  
Mehtap Y. Egrilmez ◽  
Zekiye S. Altun ◽  
Nur Arslan ◽  
Nilgun Yener ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Neuroprotective Effect ◽  
Ischemia Reperfusion ◽  
Neurovascular Unit ◽  
Glucose Deprivation ◽  
Matrix Metalloproteinase 2 ◽  
Microvascular Endothelial Cells ◽  
Oxygen Glucose Deprivation ◽  
Microvascular Endothelial

The main pathophysiology in cerebral ischemia is the structural alteration in the neurovascular unit, coinciding with neurovascular matrix degradation. Among the human matrix metalloproteinases (MMPs), MMP-2 and -9, known as gelatinases, are the key enzymes for degrading type IV collagen, which is the major component of the basal membrane that surrounds the cerebral blood vessel. In the present study, we investigated the effects of resveratrol on cytotoxicity, reactive oxygen species (ROS), and gelatinases (MMP-2 and -9) in human cerebral microvascular endothelial cells exposed to 6 hours of oxygen-glucose deprivation and a subsequent 24 hours of reoxygenation with glucose (OGD/R), to mimic ischemia/reperfusion in vivo. Lactate dehydrogenase increased significantly, in comparison to that in the normoxia group. ROS was markedly increased in the OGD/R group, compared to normoxia. Correspondingly, ROS was significantly reduced with 50 μM of resveratrol. The proMMP-2 activity in the OGD/R group showed a statistically significant increase from the control cells. Resveratrol preconditioning decreased significantly the proMMP-2 in the cells exposed to OGD/R in comparison to that in the OGD/R group. Our results indicate that resveratrol regulates MMP-2 activity induced by OGD/R via its antioxidant effect, implying a possible mechanism related to the neuroprotective effect of resveratrol.

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