scholarly journals Protein kinase A serves as a primary pathway in activation of Nur77 expression by gonadotropin-releasing hormone in the LβT2 mouse pituitary gonadotroph tumor cell line

1992 ◽  
Author(s):  
Kakar
Keyword(s):  
Protein Kinase ◽  
Tumor Cell ◽  
Protein Kinase A ◽  
Cell Line ◽  
Tumor Cell Line ◽  
Gonadotropin Releasing Hormone ◽  
Releasing Hormone ◽  
Mouse Pituitary ◽  
Kinase A

Conductive properties of a rabbit cortical collecting duct cell line: regulation by isoproterenol

1992 ◽  
Vol 262 (4) ◽  
pp. F578-F582 ◽  
Author(s):  
P. Dietl ◽  
N. Kizer ◽  
B. A. Stanton
Keyword(s):  
Protein Kinase ◽  
Protein Kinase A ◽  
Cell Line ◽  
Collecting Duct ◽  
Cortical Collecting Duct ◽  
Patch Clamp Technique ◽  
Whole Cell ◽  
Conductive Properties ◽  
Cl Conductance ◽  
Kinase A

The present study was carried out to characterize the membrane conductive properties of RCCT-28A cells, a continuous cell line derived from rabbit cortical collecting duct (CCD). RCCT-28A cells have many phenotypic properties of acid-secreting intercalated cells (A-IC). Using the whole cell patch-clamp technique, we found that the cells are conductive to Cl-, but not to Na+ or K+. The beta-adrenergic agonists isoproterenol (2 x 10(-6) M) and adenosine 3',5'-cyclic monophosphate (cAMP, 10(-4) M) increased the whole cell Cl- conductance. Protein kinase A (150 nM) in the patch pipette (i.e., intracellular solution) also increased whole cell Cl- conductance. Because isoproterenol increases cAMP levels in these cells, we conclude that isoproterenol stimulates the Cl- conductance by increasing cell cAMP, which in turn activates protein kinase A. In contrast, vasopressin does not increase cAMP in these cells and did not increase the Cl- conductance. In conclusion, these experiments show that RCCT-28A cells, like A-IC, are conductive only to Cl-. Thus RCCT-28A cells are a good model with which to study Cl- channels in the collecting duct.

Corticotropin-releasing hormone acts on guinea pig ileal smooth muscle via protein kinase A

1999 ◽  
Vol 438 (2) ◽  
pp. 205-212 ◽  
Author(s):  
D. B. Duridanova ◽  
P. S. Petkova-Kirova ◽  
L. T. Lubomirov ◽  
H. Gagov ◽  
K. Boev
Keyword(s):  
Smooth Muscle ◽  
Protein Kinase ◽  
Guinea Pig ◽  
Protein Kinase A ◽  
Corticotropin Releasing Hormone ◽  
Releasing Hormone ◽  
Kinase A

Protein Kinase Cδ as Gonadotropin-Releasing Hormone Target Isoenzyme in the αT3-1 Gonadotrope Cell Line

2004 ◽  
Vol 79 (4) ◽  
pp. 204-220 ◽  
Author(s):  
Hélène Maccario ◽  
Brice Junoy ◽  
Benoit Poulin ◽  
Bénédicte Boyer ◽  
Alain Enjalbert ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Cell Line ◽  
Gonadotropin Releasing Hormone ◽  
Releasing Hormone ◽  
Protein Kinase Cδ
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