Corticotropin-releasing hormone acts on guinea pig ileal smooth muscle via protein kinase A

1999 ◽  
Vol 438 (2) ◽  
pp. 205-212 ◽  
Author(s):  
D. B. Duridanova ◽  
P. S. Petkova-Kirova ◽  
L. T. Lubomirov ◽  
H. Gagov ◽  
K. Boev
Keyword(s):  
Smooth Muscle ◽  
Protein Kinase ◽  
Guinea Pig ◽  
Protein Kinase A ◽  
Corticotropin Releasing Hormone ◽  
Releasing Hormone ◽  
Kinase A

Beta-adrenoceptor-mediated hyperpolarization in lymphatic smooth muscle of guinea pig mesentery

1996 ◽  
Vol 270 (5) ◽  
pp. H1687-H1695 ◽  
Author(s):  
P. Y. Von der Weid ◽  
D. F. Van Helden
Keyword(s):  
Smooth Muscle ◽  
Protein Kinase ◽  
Guinea Pig ◽  
Protein Kinase A ◽  
Lymphatic Vessels ◽  
Membrane Conductance ◽  
Acetoxymethyl Ester ◽  
Nitric Oxide Synthase Inhibitor ◽  
Beta Adrenoceptor ◽  
Kinase A

Intracellular microelectrode recordings were performed to investigate the consequences of beta-adrenoceptor activation in smooth muscle of guinea pig mesenteric lymphatic vessels. Isoproterenol (Iso) hyperpolarized the membrane with an associated increase in membrane conductance and decreased the amplitude of spontaneous transient depolarizations. Iso effects were mimicked by forskolin (FSK), 3-isobutyl-1-methylxanthine, and two adenosine 3',5'-cyclic monophosphate (cAMP) derivatives. Iso- and FSK-induced hyperpolarizations were inhibited by H89, an inhibitor of cAMP-dependent protein kinase A, increased in K+-free solution, but were not affected by ouabain or by the nitric oxide synthase inhibitor N(omega)-nitro-L-arginine. They were partially inhibited by 20 mM tetraethylammonium (approximately 40%) or by 2.5 mM 4-aminopyridine (approximately 55%). The-Iso-induced hyperpolarization was partially inhibited by iberiotoxin (20 nM) and charybdotoxin (40 nM), whereas the FSK-induced hyperpolarization was less affected. In cells where the Iso-induced hyperpolarization was decreased by 40 microM 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid, acetoxymethyl ester form, the FSK-induced hyperpolarization was little changed. Our results indicate that in guinea pig mesenteric lymphatic vessels, beta-adrenoceptor stimulation activates a protein kinase A-dependent K+ conductance, involving more than one channel type.

scholarly journals Roles of Protein Kinase A (PKA) and PKC on Corticotropin-Releasing Hormone (CRH)-Induced Elevation of Cytosolic Calcium from Extra- and Intra-cellular Sources

HORMONES ◽  
2004 ◽  
Vol 3 (4) ◽  
pp. 252-258 ◽  
Author(s):  
Irini Dermitzaki ◽  
Christos Tsatsanis ◽  
Vassiliki-Ismini Alexaki ◽  
Elias Castanas ◽  
Andrew Margioris
Keyword(s):  
Protein Kinase ◽  
Protein Kinase A ◽  
Cytosolic Calcium ◽  
Corticotropin Releasing Hormone ◽  
Releasing Hormone ◽  
Kinase A

scholarly journals β-Agonist-mediated Relaxation of Airway Smooth Muscle Is Protein Kinase A-dependent

2014 ◽  
Vol 289 (33) ◽  
pp. 23065-23074 ◽  
Author(s):  
Sarah J. Morgan ◽  
Deepak A. Deshpande ◽  
Brian C. Tiegs ◽  
Anna M. Misior ◽  
Huandong Yan ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Protein Kinase ◽  
Protein Kinase A ◽  
Airway Smooth Muscle ◽  
Kinase A
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