CHARACTERIZATION OF ECTOPICALLY-EXPRESSED ETS1 IN HUMAN COLON-CANCER CELLS - INDUCTION OF PUTATIVE ETS1-TARGET GENE

1993 ◽  
Author(s):  
H SUZUKI ◽  
V ROMANOSPICA ◽  
P GEORGIOU ◽  
RJ FISHER ◽  
TS PAPAS ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Target Gene ◽  
Human Colon ◽  
Colon Cancer Cells ◽  
Human Colon Cancer ◽  
Human Colon Cancer Cells

scholarly journals Enhanced 4-Hydroxynonenal Resistance inKEAP1Silenced Human Colon Cancer Cells

2013 ◽  
Vol 2013 ◽  
pp. 1-16 ◽  
Author(s):  
Kyeong-Ah Jung ◽  
Mi-Kyoung Kwak
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Target Gene ◽  
Human Colon ◽  
Adduct Formation ◽  
Mrna Levels ◽  
Colon Cancer Cells ◽  
Human Colon Cancer ◽  
Ht29 Cells ◽  
Human Colon Cancer Cells

Nuclear factor erythroid 2-related factor 2 (NRF2) is the transcription factor that regulates an array of antioxidant/detoxifying genes for cellular defense. The conformational changes of Kelch-like ECH-associated protein 1 (KEAP1), a cytosolic repressor protein of NRF2, by various stimuli result in NRF2 liberation and accumulation in the nucleus. In the present study, we aimed to investigate the effect ofKEAP1knockdown on NRF2 target gene expression and its toxicological implication using human colon cancer cells. The stableKEAP1-knockdown HT29 cells exhibit elevated levels of NRF2 and its target gene expressions. In particular, the mRNA levels of aldo-keto reductases (AKR1C1, 1C2, 1C3, 1B1, and 1B10) were substantially increased inKEAP1silenced HT29 cells. These differential AKRs expressions appear to contribute to protection against oxidative stress. TheKEAP1-knockdown cells were relatively more resistant to hydrogen peroxide (H2O2) and 4-hydroxynonenal (4HNE) compared to the control cells. Accordantly, we observed accumulation of 4HNE protein adducts in H2O2- or 4HNE-treated control cells, whereasKEAP1-knockdown cells did not increase adduct formation. The treatment ofKEAP1-silenced cells with AKR1C inhibitor flufenamic acid increased 4HNE-induced cellular toxicity and protein adduct formation. Taken together, these results indicate that AKRs, which are NRF2-dependent highly inducible gene clusters, play a role in NRF2-mediated cytoprotection against lipid peroxide toxicity.

Characterization of HCT116 Human Colon Cancer Cells in an Orthotopic Model

2008 ◽  
Vol 147 (2) ◽  
pp. 276-281 ◽  
Author(s):  
Ashwani Rajput ◽  
Ivan Dominguez San Martin ◽  
Rebecca Rose ◽  
Alexander Beko ◽  
Charles LeVea ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Human Colon ◽  
Colon Cancer Cells ◽  
Orthotopic Model ◽  
Human Colon Cancer ◽  
Human Colon Cancer Cells
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