scholarly journals Unfavorable effect of calcitriol and its low-calcemic analogs on metastasis of 4T1 mouse mammary gland cancer

2017 ◽  
Author(s):  
Artur Anisiewicz ◽  
Agata Pawlik ◽  
Beata Filip-Psurska ◽  
Eliza Turlej ◽  
Stanisław Dzimira ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Mouse Mammary Gland ◽  
Unfavorable Effect ◽  
Mammary Gland Cancer

scholarly journals Calcitriol and Its Analogs Establish the Immunosuppressive Microenvironment That Drives Metastasis in 4T1 Mouse Mammary Gland Cancer

2018 ◽  
Vol 19 (7) ◽  
pp. 2116 ◽  
Author(s):  
Agata Pawlik ◽  
Artur Anisiewicz ◽  
Beata Filip-Psurska ◽  
Marcin Nowak ◽  
Eliza Turlej ◽  
...  
Keyword(s):  
Gene Expression ◽  
Mammary Gland ◽  
Lymph Nodes ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Cancer Metastasis ◽  
Transforming Growth Factor ◽  
Mouse Mammary Gland ◽  
Th2 Response ◽  
Mammary Gland Cancer

In our previous study, calcitriol and its analogs PRI-2191 and PRI-2205 stimulated 4T1 mouse mammary gland cancer metastasis. Therefore, we aimed to analyze the inflammatory response in 4T1-bearing mice treated with these compounds. Gene expression analysis of the splenocytes and regional lymph nodes demonstrated prevalence of the T helper lymphocytes (Th2) response with an increased activity of regulatory T (Treg) lymphocytes in mice treated with these compounds. We also observed an increased number of mature granulocytes and B lymphocytes and a decreased number of TCD4+, TCD4+CD25+, and TCD8+, as well as natural killer (NK) CD335+, cells in the blood of mice treated with calcitriol and its analogs. Among the splenocytes, we observed a significant decrease in NK CD335+ cells and an increase in TCD8+ cells. Calcitriol and its analogs decreased the levels of interleukin (IL)-1β and IL-10 and increased the level of interferon gamma (IFN-γ) in the plasma. In the tumor tissue, they caused an increase in the level of IL-10. Gene expression analysis of lung tissue demonstrated an increased level of osteopontin (Spp1) and transforming growth factor β (TGF-β) mRNA. The expression of Spp1 was also elevated in lymph nodes. Calcitriol and its analogs caused prevalence of tumor-conducive changes in the immune system of 4T1 tumor-bearing mice, despite the induction of some tumor-disadvantageous effects.

scholarly journals Temporal inhibition of mouse mammary gland cancer metastasis by CORM-A1 and DETA/NO combination therapy

Theranostics ◽  
2019 ◽  
Vol 9 (13) ◽  
pp. 3918-3939 ◽  
Author(s):  
Kseniia Porshneva ◽  
Diana Papiernik ◽  
Mateusz Psurski ◽  
Agnieszka Łupicka-Słowik ◽  
Rafał Matkowski ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Combination Therapy ◽  
Cancer Metastasis ◽  
Mouse Mammary Gland ◽  
Mammary Gland Cancer ◽  
Temporal Inhibition

Electron microscopic studies on ultrathin frozen sections of lactating mouse mammary gland

1978 ◽  
Vol 36 (2) ◽  
pp. 46-47
Author(s):  
Jan Zarzycki ◽  
Joseph Szroeder
Keyword(s):  
Mammary Gland ◽  
Protein Denaturation ◽  
Chemical Constituents ◽  
Freeze Substitution ◽  
Electron Microscopic Examination ◽  
Mouse Mammary Gland ◽  
Electron Microscopic ◽  
Preparation Methods ◽  
Microscopic Studies ◽  
Ultrathin Frozen Sections

The mammary gland ultrastructure in various functional states is the object of our investigations. The material prepared for electron microscopic examination by the conventional chemical methods has several limitations, the most important are the protein denaturation processes and the loss of large amounts of chemical constituents from the cells. In relevance to this,one can't be sure about a degree the observed images are adequate to the realy ultrastructure of a living cell. To avoid the disadvantages of the chemical preparation methods,some autors worked out alternative physical methods based on tissue freezing / freeze-drying, freeze-substitution, freeze-eatching techniqs/; actually the technique of cryoultraraicrotomy,i,e.cutting ultrathin sections from deep frozen specimens is assented as a complete alternative method. According to the limitations of the routine plastic embbeding methods we were interested to analize the mammary gland ultrastructure during lactation by the cryoultramicrotomy method.

mRNA expression of lipogenic genes in mouse mammary gland in different lactation stages

2012 ◽  
Vol 34 (3) ◽  
pp. 335-341
Author(s):  
Li-Qiang HAN ◽  
Hong-Ji LI ◽  
Yue-Ying WANG ◽  
Lin-Feng WANG ◽  
Guo-Qing YANG ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Mrna Expression ◽  
Mouse Mammary Gland ◽  
Lipogenic Genes
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