scholarly journals Altered microRNA expression profiles are involved in resistance to low-dose ionizing radiation in the absence of BMI1 in human dermal fibroblasts

2014 ◽  
Vol 45 (4) ◽  
pp. 1618-1628 ◽  
Author(s):  
SEUNGHEE BAE ◽  
KARAM KIM ◽  
HWA JUN CHA ◽  
YEONGMIN CHOI ◽  
SHANG HUN SHIN ◽  
...  
Keyword(s):  
Ionizing Radiation ◽  
Low Dose ◽  
Expression Profiles ◽  
Dermal Fibroblasts ◽  
Microrna Expression ◽  
Human Dermal Fibroblasts

scholarly journals MicroRNA expression profiles in human cancer cells after ionizing radiation

2011 ◽  
Vol 6 (1) ◽  
Author(s):  
Olivier M Niemoeller ◽  
Maximilian Niyazi ◽  
Stefanie Corradini ◽  
Franz Zehentmayr ◽  
Minglun Li ◽  
...  
Keyword(s):  
Ionizing Radiation ◽  
Cancer Cells ◽  
Human Cancer ◽  
Expression Profiles ◽  
Microrna Expression ◽  
Human Cancer Cells

scholarly journals Circular RNA expression profiles significantly altered in UVA‑irradiated human dermal fibroblasts

2020 ◽  
Vol 20 (6) ◽  
pp. 1-1
Author(s):  
Mengbi Lin ◽  
Yue Zheng ◽  
Qian Li ◽  
Yufang Liu ◽  
Qingfang Xu ◽  
...  
Keyword(s):  
Expression Profiles ◽  
Circular Rna ◽  
Dermal Fibroblasts ◽  
Rna Expression ◽  
Human Dermal Fibroblasts

scholarly journals Whole Transcriptome Analyses Reveal Differential mRNA and microRNA Expression Profiles in Primary Human Dermal Fibroblasts Infected with Clinical or Vaccine Strains of Varicella Zoster Virus

Pathogens ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 183 ◽  
Author(s):  
Oh ◽  
Lim ◽  
Song ◽  
Ahn ◽  
Lee ◽  
...  
Keyword(s):  
Varicella Zoster Virus ◽  
Differentially Expressed Genes ◽  
Expression Profiles ◽  
Expression Patterns ◽  
Dermal Fibroblasts ◽  
Differentially Expressed ◽  
Small Rna Sequencing ◽  
Human Dermal Fibroblasts ◽  
Varicella Zoster ◽  
Low Passage

Licensed live attenuated vaccines have been developed to prevent varicella zoster virus (VZV) infection, which causes chickenpox and shingles. The genomic sequences of both clinical- and vaccine-derived VZV strains have been analyzed previously. To further characterize the molecular signatures and complexity of wildtype (clinical) versus attenuated (vaccine-derived) VZV-mediated host cellular responses, we performed high-throughput next generation sequencing to quantify and compare the expression patterns of mRNAs and microRNAs (miRNAs) in primary human dermal fibroblasts (HDFs) infected with wildtype (YC01 low passage) and attenuated (YC01 high passage, SuduVax, and VarilRix) VZV strains. 3D-multidimensional scaling of the differentially expressed genes demonstrated the distinct grouping of wildtype and attenuated strains. In particular, we observed that HDFs infected with attenuated strains had more differentially expressed genes (DEGs) involved in the retinoic-acid inducible gene–I-like receptor and interferon-mediated signaling pathways compared with wildtype strains. Additionally, miRNA expression patterns were profiled following the infection of HDFs with VZV. Small RNA sequencing identified that several miRNAs were upregulated, including miR-146a-5p, which has been associated with other herpesvirus infections, whereas let-7a-3p was downregulated in both wildtype and attenuated VZV-infected cells. This study identified genes and miRNAs that may be essential in VZV pathogenesis.

Low dose stingless bee honey increases viability of human dermal fibroblasts that could potentially promote wound healing

2018 ◽  
Vol 23 ◽  
pp. 22-27 ◽  
Author(s):  
Abid Nordin ◽  
Norazzila Omar ◽  
Nur Qisya Afifah Veronica Sainik ◽  
Shiplu Roy Chowdhury ◽  
Eshaifol Omar ◽  
...  
Keyword(s):  
Wound Healing ◽  
Low Dose ◽  
Stingless Bee ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Promote Wound Healing

scholarly journals Ionizing radiation exposure of stem cell-derived chondrocytes affects their gene and microRNA expression profiles and cytokine production

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ewelina Stelcer ◽  
Katarzyna Kulcenty ◽  
Marcin Rucinski ◽  
Marta Kruszyna-Mochalska ◽  
Agnieszka Skrobala ◽  
...  
Keyword(s):  
Dna Damage ◽  
Ionizing Radiation ◽  
Expression Profile ◽  
Cellular Response ◽  
Expression Profiles ◽  
Microrna Expression ◽  
Biological Processes ◽  
Microrna Expression Profile ◽  
Differentiated Cells ◽  
Induced Pluripotent

AbstractHuman induced pluripotent stem cells (hiPSCs) can be differentiated into chondrocyte-like cells. However, implantation of these cells is not without risk given that those transplanted cells may one day undergo ionizing radiation (IR) in patients who develop cancer. We aimed to evaluate the effect of IR on chondrocyte-like cells differentiated from hiPSCs by determining their gene and microRNA expression profile and proteomic analysis. Chondrocyte-like cells differentiated from hiPSCs were placed in a purpose-designed phantom to model laryngeal cancer and irradiated with 1, 2, or 3 Gy. High-throughput analyses were performed to determine the gene and microRNA expression profile based on microarrays. The composition of the medium was also analyzed. The following essential biological processes were activated in these hiPSC-derived chondrocytes after IR: "apoptotic process", "cellular response to DNA damage stimulus", and "regulation of programmed cell death". These findings show the microRNAs that are primarily responsible for controlling the genes of the biological processes described above. We also detected changes in the secretion level of specific cytokines. This study demonstrates that IR activates DNA damage response mechanisms in differentiated cells and that the level of activation is a function of the radiation dose.

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