scholarly journals Pentosan polysulfate ameliorates apoptosis and inflammation by suppressing activation of the p38 MAPK pathway in high glucose‑treated HK‑2 cells

2017 ◽  
Author(s):  
Ping Chen ◽  
Yang Yuan ◽  
Tianying Zhang ◽  
Bo Xu ◽  
Qing Gao ◽  
...  
Keyword(s):  
P38 Mapk ◽  
High Glucose ◽  
Mapk Pathway ◽  
Pentosan Polysulfate ◽  
P38 Mapk Pathway ◽  
Apoptosis And Inflammation

scholarly journals FR167653 inhibits fibronectin expression and apoptosis in diabetic glomeruli and in high-glucose-stimulated mesangial cells

2008 ◽  
Vol 295 (2) ◽  
pp. F595-F604 ◽  
Author(s):  
Dong-Sub Jung ◽  
Jin Ji Li ◽  
Seung-Jae Kwak ◽  
Sun Ha Lee ◽  
Jehyun Park ◽  
...  
Keyword(s):  
Diabetic Nephropathy ◽  
Protein Expression ◽  
P38 Mapk ◽  
High Glucose ◽  
Mesangial Cells ◽  
Mapk Pathway ◽  
P38 Mapk Pathway ◽  
Fibronectin Expression

Previous in vitro studies suggest that the p38 MAPK pathway may be involved in the pathogenesis of diabetic nephropathy, but the consequences of the inhibition of the p38 MAPK pathway have not been well elucidated in diabetic (DM) glomeruli. This study was undertaken to investigate the effect of p38 MAPK inhibitor, FR167653, on fibronectin expression and apoptosis in DM glomeruli and in high-glucose-stimulated mesangial cells (MC). In vivo, 32 Sprague-Dawley rats were injected with diluent (control, N = 16) or streptozotocin intraperitoneally (DM, N = 16). Eight rats from each group were treated with FR167653 for 3 mo. In vitro, rat MC were exposed to medium containing 5.6 mM glucose or 30 mM glucose [high glucose (HG)] with or without 10−6 M FR167653 for 24 h. Fibronectin mRNA and protein expression were determined by real-time PCR and Western blot, respectively. Western blot for apoptosis-related molecules, terminal deoxynucleotidyl transferase dUTP-mediated nick-end labeling assay, and Hoechst 33342 staining were performed to determine apoptosis. FR167653 ameliorated the increases in fibronectin-to-GAPDH mRNA ratio and protein expression in DM glomeruli by 89 and 79% and in HG-stimulated MC by 70 and 91%, respectively ( P < 0.05). Under diabetic conditions, Bcl-2 protein expression was decreased, whereas cleaved caspase-3 protein expression was increased ( P < 0.05), and these changes were inhibited by FR167653 treatment. Apoptotic cells were also significantly increased in DM glomeruli and in HG-stimulated MC ( P < 0.05), and FR167653 ameliorated these increases in apoptotic cells, both in vivo and in vitro. In conclusion, these findings suggest that the inhibition of the p38 MAPK pathway has a beneficial effect on the development of diabetic nephropathy by inhibiting the increase in fibronectin expression and apoptosis.

scholarly journals p38 MAPK pathway is involved in high glucose-induced thioredoxin interacting protein induction in mouse mesangial cells

FEBS Letters ◽  
2010 ◽  
Vol 584 (15) ◽  
pp. 3480-3485 ◽  
Author(s):  
Yunzhuo Ren ◽  
Yonghong Shi ◽  
Yuehua Wang ◽  
Yingmin Li ◽  
Shuhui Wu ◽  
...  
Keyword(s):  
P38 Mapk ◽  
High Glucose ◽  
Mesangial Cells ◽  
Mapk Pathway ◽  
Interacting Protein ◽  
Thioredoxin Interacting Protein ◽  
P38 Mapk Pathway

scholarly journals High glucose activates the p38 MAPK pathway in cultured human peritoneal mesothelial cells

2003 ◽  
Vol 63 (3) ◽  
pp. 958-968 ◽  
Author(s):  
Zhong-Gao Xu ◽  
Kyung Sik Kim ◽  
Hyeong Cheon Park ◽  
Kyu Hun Choi ◽  
Ho Yung Lee ◽  
...  
Keyword(s):  
P38 Mapk ◽  
High Glucose ◽  
Mapk Pathway ◽  
Mesothelial Cells ◽  
Peritoneal Mesothelial Cells ◽  
P38 Mapk Pathway ◽  
Human Peritoneal Mesothelial Cells

Role of the p38 MAPK Pathway in Induction of iNOS Expression in Human Leukocytes

2008 ◽  
Vol 56 (1) ◽  
pp. 83-89 ◽  
Author(s):  
Ewa Jablonska ◽  
Wioletta Ratajczak ◽  
Jakub Jablonski
Keyword(s):  
P38 Mapk ◽  
Mapk Pathway ◽  
Inos Expression ◽  
Human Leukocytes ◽  
P38 Mapk Pathway
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