scholarly journals [Comment] MALDI‑TOF MS‑based direct‑on‑target microdroplet growth assay: Latest developments

2020 ◽  
Author(s):  
Ioannis Neonakis ◽  
Demetrios Spandidos
Keyword(s):  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Growth Assay ◽  
Tof Ms

scholarly journals Development of a MALDI-TOF MS-based screening panel for accelerated differential detection of carbapenemases in Enterobacterales using the direct-on-target microdroplet growth assay

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Carlos L. Correa-Martínez ◽  
Evgeny A. Idelevich ◽  
Katrin Sparbier ◽  
Thorsten Kuczius ◽  
Markus Kostrzewa ◽  
...  
Keyword(s):  
Differential Detection ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Growth Assay ◽  
Tof Ms

scholarly journals Rapid Simultaneous Testing of Multiple Antibiotics by the MALDI-TOF MS Direct-on-Target Microdroplet Growth Assay

Diagnostics ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 1803
Author(s):  
Evgeny A. Idelevich ◽  
Ilka D. Nix ◽  
Janika A. Busch ◽  
Katrin Sparbier ◽  
Oliver Drews ◽  
...  
Keyword(s):  
Growth Control ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Assay Performance ◽  
Microbiological Methods ◽  
Humidity Chamber ◽  
Growth Assay ◽  
Technical Solutions ◽  
Growth Controls ◽  
Tof Ms

Accelerating antimicrobial susceptibility testing (AST) is a priority in the development of novel microbiological methods. The MALDI-TOF MS-based direct-on-target microdroplet growth assay (DOT-MGA) has recently been described as a rapid phenotypic AST method. In this proof-of-principle study, we expanded this method to simultaneously test 24 antimicrobials. An Enterobacterales panel was designed and evaluated using 24 clinical isolates. Either one or two (only for antimicrobials with the EUCAST “I” category) breakpoint concentrations were tested. Microdroplets containing bacterial suspensions with antimicrobials and growth controls were incubated directly on the spots of a disposable MALDI target inside a humidity chamber for 6, 8 or 18 h. Broth microdilution was used as the standard method. After 6 and 8 h of incubation, the testing was valid (i.e., growth control was successfully detected) for all isolates and the overall categorical agreement was 92.0% and 92.7%, respectively. Although the overall assay performance applying short incubation times is promising, the lower performance with some antimicrobials and when using the standard incubation time of 18 h indicates the need for thorough standardization of assay conditions. While using “homebrew” utensils and provisional evaluation algorithms here, technical solutions such as dedicated incubation chambers, tools for broth removal and improved software analyses are needed.

895: Proteomic Analysis of Sera from Bladder Cancer Patients with MALDI-TOF-MS

2007 ◽  
Vol 177 (4S) ◽  
pp. 297-297
Author(s):  
Kristina Schwamborn ◽  
Rene Krieg ◽  
Ruth Knüchel-Clarke ◽  
Joachim Grosse ◽  
Gerhard Jakse
Keyword(s):  
Bladder Cancer ◽  
Cancer Patients ◽  
Proteomic Analysis ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

HPTLC-MALDI/TOF/MS vs. HPLC-ESI/MS2 for identification to flavonoids in plant extracts

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
L Fougère ◽  
D Da Silva ◽  
E Destandau ◽  
C Elfakir
Keyword(s):  
Plant Extracts ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

Infektionserreger beim Schwein – Analyse des kultivierbaren Mikrobioms mittels MALDI-TOF MS

2017 ◽  
Author(s):  
M Erhard ◽  
M Metzner ◽  
D Köhler-Repp ◽  
B Köhler ◽  
R Storandt
Keyword(s):  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

Quantitative metabolomics of Iranian saffron based on their HPLC-DAD and MALDI-TOF-MS

2019 ◽  
Author(s):  
M Hooshyari ◽  
H Rezadoost ◽  
P Ghezellou ◽  
A Ghassempour
Keyword(s):  
Maldi Tof Ms ◽  
Quantitative Metabolomics ◽  
Maldi Tof ◽  
Tof Ms
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