scholarly journals Rapid Detection of Extended-Spectrum β-Lactamases (ESBL) and AmpC β-Lactamases in Enterobacterales: Development of a Screening Panel Using the MALDI-TOF MS-Based Direct-on-Target Microdroplet Growth Assay

2019 ◽  
Vol 10 ◽  
Author(s):  
Carlos L. Correa-Martínez ◽  
Evgeny A. Idelevich ◽  
Katrin Sparbier ◽  
Markus Kostrzewa ◽  
Karsten Becker
Keyword(s):  
Rapid Detection ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Extended Spectrum ◽  
Growth Assay ◽  
Tof Ms

Investigation of High-Risk ST131 Clone in Extended Spectrum β-Lactamase–Producing Escherichia coli Isolates in Children

2021 ◽  
Author(s):  
Mehmet E. Bulut ◽  
Gülen Hürkal ◽  
Nazan Dalgıç
Keyword(s):  
Escherichia Coli ◽  
High Risk ◽  
Pediatric Patients ◽  
Pediatric Population ◽  
Maldi Tof Ms ◽  
E Coli ◽  
Maldi Tof ◽  
Extended Spectrum ◽  
St131 Clone ◽  
Tof Ms

Abstract Objective Antimicrobial resistance poses a serious threat to children's health. In recent years, high-risk Escherichia coli ST131 has become an important target for global surveillance studies. The E.coli ST131 clone is associated with extended spectrum β-lactamase (ESBL) production, as well as multidrug resistance and treatment failure. Studies on this clone in the pediatric age group are limited. We aim to investigate the rate of high-risk E. coli ST131 clone in ESBL-positive E. coli isolates obtained from pediatric patients. Methods A total of 292 ESBL-positive E. coli isolates from clinical samples of pediatric patients was included in the study. MALDI-TOF MS system was used for bacterial identification. Susceptibility tests were performed using BD Phoenix automated system. ST131 detection was done by MALDI-TOF-MS. Fisher's exact test was used to compare the groups (significance <0.05). Results A total of 292 isolates was analyzed. The high-risk ST131 clone was detected in 117 (40%) of the 292 ESBL-positive isolates. ST131 rates were found to be significantly higher in children under the age of 5 years compared with children over the age of 5 years (49.3 vs. 31.1%, p = 0.0019). Ciprofloxacin resistance was higher in ST131 isolates (45.6 vs. 31.7%; p < 0.05). Conclusion The rate of the ST131 clone was found to be high in the pediatric population. The significantly high rate of resistance to ciprofloxacin, which is not commonly used in the pediatric population, in ST131 isolates reveals the importance of the spread of high-risk clones for the development of resistance.

scholarly journals Stable isotope labelling: an approach for MALDI-TOF MS-based rapid detection of fluconazole resistance in Candida tropicalis

2019 ◽  
Vol 74 (5) ◽  
pp. 1269-1276 ◽  
Author(s):  
Saikat Paul ◽  
Shreya Singh ◽  
Arunaloke Chakrabarti ◽  
Shivaprakash M Rudramurthy ◽  
Anup K Ghosh
Keyword(s):  
Stable Isotope ◽  
Candida Tropicalis ◽  
Rapid Detection ◽  
Stable Isotope Labelling ◽  
Fluconazole Resistance ◽  
Isotope Labelling ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

scholarly journals Development of a MALDI-TOF MS-based screening panel for accelerated differential detection of carbapenemases in Enterobacterales using the direct-on-target microdroplet growth assay

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Carlos L. Correa-Martínez ◽  
Evgeny A. Idelevich ◽  
Katrin Sparbier ◽  
Thorsten Kuczius ◽  
Markus Kostrzewa ◽  
...  
Keyword(s):  
Differential Detection ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Growth Assay ◽  
Tof Ms

Rapid detection and identification of strains carrying carbapenemases directly from positive blood cultures using MALDI-TOF MS

2014 ◽  
Vol 105 ◽  
pp. 98-101 ◽  
Author(s):  
Y. Hoyos-Mallecot ◽  
C. Riazzo ◽  
C. Miranda-Casas ◽  
M.D. Rojo-Martín ◽  
J. Gutiérrez-Fernández ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Blood Cultures ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Detection And Identification ◽  
Tof Ms

Rapid detection of Lactococcus lactis isolates producing the lantibiotics nisin, lacticin 481 and lacticin 3147 using MALDI-TOF MS

2017 ◽  
Vol 139 ◽  
pp. 138-142 ◽  
Author(s):  
Tomás García-Cayuela ◽  
Teresa Requena ◽  
M. Carmen Martínez-Cuesta ◽  
Carmen Peláez
Keyword(s):  
Lactococcus Lactis ◽  
Rapid Detection ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Lacticin 3147 ◽  
Lacticin 481 ◽  
Tof Ms
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