scholarly journals Novel near‑infrared fluorescent probe for live cell imaging

2019 ◽  
Author(s):  
Meng Wan ◽  
Yubing Zhu ◽  
Jianjun Zou
Keyword(s):  
Fluorescent Probe ◽  
Live Cell Imaging ◽  
Near Infrared ◽  
Cell Imaging ◽  
Live Cell

A lysosome-targetable near infrared fluorescent probe for glutathione sensing and live-cell imaging

2019 ◽  
Vol 301 ◽  
pp. 127065 ◽  
Author(s):  
Ziming Zheng ◽  
Yuchen Huyan ◽  
Hongjuan Li ◽  
Shiguo Sun ◽  
Yongqian Xu
Keyword(s):  
Fluorescent Probe ◽  
Live Cell Imaging ◽  
Near Infrared ◽  
Cell Imaging ◽  
Live Cell

scholarly journals A Near‐Infrared Photoswitchable Protein–Fluorophore Tag for No‐Wash Live Cell Imaging

2018 ◽  
Vol 130 (49) ◽  
pp. 16315-16319 ◽  
Author(s):  
Wei Sheng ◽  
Setare Tahmasebi Nick ◽  
Elizabeth M. Santos ◽  
Xinliang Ding ◽  
Jun Zhang ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Near Infrared ◽  
Cell Imaging ◽  
Live Cell

scholarly journals Optimization of Advanced Live-Cell Imaging through Red/Near-Infrared Dye Labeling and Fluorescence Lifetime-Based Strategies

2021 ◽  
Vol 22 (20) ◽  
pp. 11092
Author(s):  
Magalie Bénard ◽  
Damien Schapman ◽  
Christophe Chamot ◽  
Fatéméh Dubois ◽  
Guénaëlle Levallet ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Live Cell Imaging ◽  
Near Infrared ◽  
Cell Imaging ◽  
Light Exposure ◽  
Photon Counting ◽  
Live Cell ◽  
Stimulated Emission ◽  
Plot Analysis ◽  
Phasor Plot

Fluorescence microscopy is essential for a detailed understanding of cellular processes; however, live-cell preservation during imaging is a matter of debate. In this study, we proposed a guide to optimize advanced light microscopy approaches by reducing light exposure through fluorescence lifetime (τ) exploitation of red/near-infrared dyes. Firstly, we characterized key instrumental elements which revealed that red/near-infrared laser lines with an 86x (Numerical Aperture (NA) = 1.2, water immersion) objective allowed high transmission of fluorescence signals, low irradiance and super-resolution. As a combination of two technologies, i.e., vacuum tubes (e.g., photomultiplier) and semiconductor microelectronics (e.g., avalanche photodiode), type S, X and R of hybrid detectors (HyD-S, HyD-X and HyD-R) were particularly adapted for red/near-infrared photon counting and τ separation. Secondly, we tested and compared lifetime-based imaging including coarse τ separation for confocal microscopy, fitting and phasor plot analysis for fluorescence lifetime microscopy (FLIM), and lifetimes weighting for enhanced stimulated emission depletion (STED) nanoscopy, in light of red/near-infrared multiplexing. Mainly, we showed that the choice of appropriate imaging approach may depend on fluorochrome number, together with their spectral/lifetime characteristics and STED compatibility. Photon-counting mode and sensitivity of HyDs together with phasor plot analysis of fluorescence lifetimes enabled the flexible and fast imaging of multi-labeled living H28 cells. Therefore, a combination of red/near-infrared dyes labeling with lifetime-based strategies offers new perspectives for live-cell imaging by enhancing sample preservation through acquisition time and light exposure reduction.

A new ESIPT-based fluorescent probe for highly selective and sensitive detection of hydrogen sulfide and its application in live-cell imaging

2017 ◽  
Vol 41 (3) ◽  
pp. 1119-1123 ◽  
Author(s):  
Bo Chen ◽  
Jing Huang ◽  
Huiqing Geng ◽  
Lingli Xuan ◽  
Tengfei Xu ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Fluorescent Probe ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Sensitive Detection

A new ESIPT-based fluorescent probe for the sensitive detection of hydrogen sulfide has been reasonably designed and developed.

scholarly journals NitroxylFluor: A Thiol-Based Fluorescent Probe for Live-Cell Imaging of Nitroxyl

2017 ◽  
Vol 139 (51) ◽  
pp. 18476-18479 ◽  
Author(s):  
Nicholas W. Pino ◽  
Jerome Davis ◽  
Zhengxin Yu ◽  
Jefferson Chan
Keyword(s):  
Fluorescent Probe ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell

A thiocoumarin-based turn-on fluorescent probe for hypochlorite detection and its application to live-cell imaging

2020 ◽  
Vol 317 ◽  
pp. 128213 ◽  
Author(s):  
Van-Nghia Nguyen ◽  
Seonye Heo ◽  
Sangin Kim ◽  
K.M.K. Swamy ◽  
Jeongsun Ha ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Turn On

Near-Infrared-Emitting Iridium(III) Complexes as Phosphorescent Dyes for Live Cell Imaging

2013 ◽  
Vol 33 (1) ◽  
pp. 61-68 ◽  
Author(s):  
Guoliang Zhang ◽  
Huiyuan Zhang ◽  
Yuan Gao ◽  
Ran Tao ◽  
Lijun Xin ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Near Infrared ◽  
Cell Imaging ◽  
Live Cell ◽  
Phosphorescent Dyes

An ESIPT-based fluorescent probe for selective detection of homocysteine and its application in live-cell imaging

2016 ◽  
Vol 57 (47) ◽  
pp. 5227-5231 ◽  
Author(s):  
Lijun Tang ◽  
Jianze Shi ◽  
Zhenlong Huang ◽  
Xiaomei Yan ◽  
Qiang Zhang ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Selective Detection
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