scholarly journals A Near‐Infrared Photoswitchable Protein–Fluorophore Tag for No‐Wash Live Cell Imaging

2018 ◽  
Vol 130 (49) ◽  
pp. 16315-16319 ◽  
Author(s):  
Wei Sheng ◽  
Setare Tahmasebi Nick ◽  
Elizabeth M. Santos ◽  
Xinliang Ding ◽  
Jun Zhang ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Near Infrared ◽  
Cell Imaging ◽  
Live Cell

scholarly journals Optimization of Advanced Live-Cell Imaging through Red/Near-Infrared Dye Labeling and Fluorescence Lifetime-Based Strategies

2021 ◽  
Vol 22 (20) ◽  
pp. 11092
Author(s):  
Magalie Bénard ◽  
Damien Schapman ◽  
Christophe Chamot ◽  
Fatéméh Dubois ◽  
Guénaëlle Levallet ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Live Cell Imaging ◽  
Near Infrared ◽  
Cell Imaging ◽  
Light Exposure ◽  
Photon Counting ◽  
Live Cell ◽  
Stimulated Emission ◽  
Plot Analysis ◽  
Phasor Plot

Fluorescence microscopy is essential for a detailed understanding of cellular processes; however, live-cell preservation during imaging is a matter of debate. In this study, we proposed a guide to optimize advanced light microscopy approaches by reducing light exposure through fluorescence lifetime (τ) exploitation of red/near-infrared dyes. Firstly, we characterized key instrumental elements which revealed that red/near-infrared laser lines with an 86x (Numerical Aperture (NA) = 1.2, water immersion) objective allowed high transmission of fluorescence signals, low irradiance and super-resolution. As a combination of two technologies, i.e., vacuum tubes (e.g., photomultiplier) and semiconductor microelectronics (e.g., avalanche photodiode), type S, X and R of hybrid detectors (HyD-S, HyD-X and HyD-R) were particularly adapted for red/near-infrared photon counting and τ separation. Secondly, we tested and compared lifetime-based imaging including coarse τ separation for confocal microscopy, fitting and phasor plot analysis for fluorescence lifetime microscopy (FLIM), and lifetimes weighting for enhanced stimulated emission depletion (STED) nanoscopy, in light of red/near-infrared multiplexing. Mainly, we showed that the choice of appropriate imaging approach may depend on fluorochrome number, together with their spectral/lifetime characteristics and STED compatibility. Photon-counting mode and sensitivity of HyDs together with phasor plot analysis of fluorescence lifetimes enabled the flexible and fast imaging of multi-labeled living H28 cells. Therefore, a combination of red/near-infrared dyes labeling with lifetime-based strategies offers new perspectives for live-cell imaging by enhancing sample preservation through acquisition time and light exposure reduction.

Near-Infrared-Emitting Iridium(III) Complexes as Phosphorescent Dyes for Live Cell Imaging

2013 ◽  
Vol 33 (1) ◽  
pp. 61-68 ◽  
Author(s):  
Guoliang Zhang ◽  
Huiyuan Zhang ◽  
Yuan Gao ◽  
Ran Tao ◽  
Lijun Xin ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Near Infrared ◽  
Cell Imaging ◽  
Live Cell ◽  
Phosphorescent Dyes

scholarly journals A NIR-emitting cyanine with large Stokes shifts for live cell imaging: large impact of the phenol group on emission

2019 ◽  
Vol 55 (88) ◽  
pp. 13223-13226 ◽  
Author(s):  
Dipendra Dahal ◽  
Lucas McDonald ◽  
Sabita Pokhrel ◽  
Sailaja Paruchuri ◽  
Michael Konopka ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Near Infrared ◽  
Cell Imaging ◽  
Live Cell ◽  
Molecular Probe ◽  
Large Impact ◽  
Phenol Group ◽  
Stokes Shifts ◽  
Excellent Selectivity

A near-infrared (NIR) emitting (λem = 700–900 nm) molecular probe exhibits excellent selectivity towards mitochondria for live-cell imaging.

scholarly journals Near-infrared probes based on fluorinated Si-rhodamine for live cell imaging

RSC Advances ◽  
2017 ◽  
Vol 7 (18) ◽  
pp. 10922-10927 ◽  
Author(s):  
Suxia Shen ◽  
Jingru Yu ◽  
Yaomin Lu ◽  
Shuchen Zhang ◽  
Xuegang Yi ◽  
...  
Keyword(s):  
Phenyl Ring ◽  
Live Cell Imaging ◽  
Near Infrared ◽  
Cell Imaging ◽  
Live Cell ◽  
Trifluoromethyl Group ◽  
High Brightness ◽  
Infrared Probes ◽  
Excellent Stability

Si-rhodamine probe with a trifluoromethyl group on the 2-position of the pendant phenyl ring retains high brightness and excellent stability in a harsh physiological environment.

scholarly journals Rücktitelbild: Polynuclear SmIIIPolyamidoamine-Based Dendrimer: A Single Probe for Combined Visible and Near-Infrared Live-Cell Imaging (Angew. Chem. 11/2014)

2014 ◽  
Vol 126 (11) ◽  
pp. 3094-3094
Author(s):  
Alexandra Foucault-Collet ◽  
Chad M. Shade ◽  
Iuliia Nazarenko ◽  
Stéphane Petoud ◽  
Svetlana V. Eliseeva
Keyword(s):  
Live Cell Imaging ◽  
Near Infrared ◽  
Cell Imaging ◽  
Live Cell ◽  
Single Probe

A lysosome-targetable near infrared fluorescent probe for glutathione sensing and live-cell imaging

2019 ◽  
Vol 301 ◽  
pp. 127065 ◽  
Author(s):  
Ziming Zheng ◽  
Yuchen Huyan ◽  
Hongjuan Li ◽  
Shiguo Sun ◽  
Yongqian Xu
Keyword(s):  
Fluorescent Probe ◽  
Live Cell Imaging ◽  
Near Infrared ◽  
Cell Imaging ◽  
Live Cell

Fluorogenic Protein Probes with Red or Near-Infrared Emission for Genetically Targeted Live-Cell Imaging

2020 ◽  
Author(s):  
Sylvestre P. J. T. Bachollet ◽  
Cyril Addi ◽  
Jean-Maurice Mallet ◽  
Blaise Dumat
Keyword(s):  
Live Cell Imaging ◽  
Near Infrared ◽  
Cell Imaging ◽  
Infrared Emission ◽  
Live Cell ◽  
Molecular Rotor ◽  
Model Protein ◽  
Near Infrared Emission

A series of red-emitting and near-infrared fluorogenic protein probes based on push-pull molecular rotor structures was developed. After characterization of their optical properties using Bovine Serum Albumin as a model protein, they were conjugated to a halogenoalkane ligand in order to target the protein self-labeling tag HaloTag. The interaction with HaloTag was investigated in vitro and then the most promising probes were applied to live-cell imaging in wash-free conditions using fluorogenic and chemogenetic targeting of HaloTag fusion proteins.<br>

Polynuclear SmIIIPolyamidoamine-Based Dendrimer: A Single Probe for Combined Visible and Near-Infrared Live-Cell Imaging

2014 ◽  
Vol 126 (11) ◽  
pp. 2971-2974 ◽  
Author(s):  
Alexandra Foucault-Collet ◽  
Chad M. Shade ◽  
Iuliia Nazarenko ◽  
Stéphane Petoud ◽  
Svetlana V. Eliseeva
Keyword(s):  
Live Cell Imaging ◽  
Near Infrared ◽  
Cell Imaging ◽  
Live Cell ◽  
Single Probe

scholarly journals A Near-Infrared Fluorogenic Pyrrole–Imidazole Polyamide Probe for Live-Cell Imaging of Telomeres

2020 ◽  
Vol 142 (41) ◽  
pp. 17356-17363 ◽  
Author(s):  
Yutaro Tsubono ◽  
Yusuke Kawamoto ◽  
Takuya Hidaka ◽  
Ganesh N. Pandian ◽  
Kaori Hashiya ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Near Infrared ◽  
Cell Imaging ◽  
Live Cell
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