scholarly journals Cardiotoxicity and lung toxicity in male rats induced by long‑term exposure to iron oxide and silver nanoparticles

2019 ◽  
Author(s):  
Mokhtar Yousef ◽  
Abdelsalam Abuzreda ◽  
Maher Kamel
Keyword(s):  
Silver Nanoparticles ◽  
Iron Oxide ◽  
Lung Toxicity ◽  
Male Rats

scholarly journals Mathematical Assessment of Results of Investigation of the BCL-2 and Caspase-3 Protein Expression under the Influence of nanobiocomposites of Silver on natural and Synthetic Matrixes

2019 ◽  
Vol 3 (6) ◽  
pp. 150-155
Author(s):  
M. A. Novikov ◽  
E. A. Titov ◽  
V. A. Vokina ◽  
L. M. Sosedova
Keyword(s):  
Silver Nanoparticles ◽  
Discriminant Analysis ◽  
Caspase 3 ◽  
Early Period ◽  
Feature Space ◽  
Male Rats ◽  
Apoptotic Proteins ◽  
Comparison Of The Results ◽  
Two Stages

The aim was to assess the effect of silver nanoparticles on the expression of proand anti-apoptotic proteins caspase-3 and bcl-2 by discriminant analysis.Materials and methods. 120 sexually mature outbred male rats were divided into 8 groups (pure arabinogalactan (AG), pure poly-1-vinyl-1,2,4-triazol (PVT), nanobiocomposites on the AG and PVT matrix at a dose of 100 and 500 μg/kg. The administration was done orally for 9 days. The experimental study was carried out in two stages. The first stage included the examination of animals immediately after the end of the exposure of the studied substances (early period), the second stage – a survey 6 months after the end of the exposure (the long-term period).Results. The most distinguished groups were the groups that were administered silver nanoparticles on the AG matrix at a dose of 100 and 500 μg/kg.Conclusion. Comparison of the results of the discriminant analysis allowed to fully determine the effect of silver nanoparticles on the expression of proand anti-apoptotic proteins caspase-3 and bcl-2 when they were introduced on the AG and PVT matrix. In the AG groups and nanobiocomposites based on it, in the feature space, it was shown that the most remote by differentiating characteristics are the groups nAG100 and nAG500. In nanocomposites on the PVT matrix, a group of animals differing in differentiating features has not been identified.

scholarly journals Reproductive toxicity of iron oxide nanoparticles, silver nanoparticles and their mixture in male rats: Effects on testicular gene expression

2020 ◽  
Vol 7 (2) ◽  
pp. 075-081
Author(s):  
Ahmed Ibrahim Younus ◽  
Mokhtar Ibrahim Yousef ◽  
Kamel Maher Abdel-Nabi ◽  
Mohammed Ibrahim Younus ◽  
Jubran Mohammed Abdulrahman
Keyword(s):  
Gene Expression ◽  
Silver Nanoparticles ◽  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Reproductive Toxicity ◽  
Male Rats ◽  
Oxide Nanoparticles

scholarly journals Testosterone activates glucose metabolism through AMPK and androgen signaling in cardiomyocyte hypertrophy

2021 ◽  
Vol 54 (1) ◽  
Author(s):  
Mayarling Francisca Troncoso ◽  
Mario Pavez ◽  
Carlos Wilson ◽  
Daniel Lagos ◽  
Javier Duran ◽  
...  
Keyword(s):  
Glucose Metabolism ◽  
Cardiac Hypertrophy ◽  
Glucose Uptake ◽  
Male Rats ◽  
Cardiomyocyte Hypertrophy ◽  
Mrna Levels ◽  
Elevated Glucose ◽  
Amp Activated Protein Kinase ◽  
Cultured Cardiomyocytes

Abstract Background Testosterone regulates nutrient and energy balance to maintain protein synthesis and metabolism in cardiomyocytes, but supraphysiological concentrations induce cardiac hypertrophy. Previously, we determined that testosterone increased glucose uptake—via AMP-activated protein kinase (AMPK)—after acute treatment in cardiomyocytes. However, whether elevated glucose uptake is involved in long-term changes of glucose metabolism or is required during cardiomyocyte growth remained unknown. In this study, we hypothesized that glucose uptake and glycolysis increase in testosterone-treated cardiomyocytes through AMPK and androgen receptor (AR). Methods Cultured cardiomyocytes were stimulated with 100 nM testosterone for 24 h, and hypertrophy was verified by increased cell size and mRNA levels of β-myosin heavy chain (β-mhc). Glucose uptake was assessed by 2-NBDG. Glycolysis and glycolytic capacity were determined by measuring extracellular acidification rate (ECAR). Results Testosterone induced cardiomyocyte hypertrophy that was accompanied by increased glucose uptake, glycolysis enhancement and upregulated mRNA expression of hexokinase 2. In addition, testosterone increased AMPK phosphorylation (Thr172), while inhibition of both AMPK and AR blocked glycolysis and cardiomyocyte hypertrophy induced by testosterone. Moreover, testosterone supplementation in adult male rats by 5 weeks induced cardiac hypertrophy and upregulated β-mhc, Hk2 and Pfk2 mRNA levels. Conclusion These results indicate that testosterone stimulates glucose metabolism by activation of AMPK and AR signaling which are critical to induce cardiomyocyte hypertrophy.

The timing of maternal separation affects morris water maze performance and long-term potentiation in male rats

2013 ◽  
Vol 56 (5) ◽  
pp. 1102-1109 ◽  
Author(s):  
Xiujing Cao ◽  
Shenghai Huang ◽  
Jiejie Cao ◽  
Tingting Chen ◽  
Ping Zhu ◽  
...  
Keyword(s):  
Morris Water Maze ◽  
Water Maze ◽  
Maternal Separation ◽  
Long Term Potentiation ◽  
Male Rats ◽  
Maze Performance ◽  
Term Potentiation
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