scholarly journals Mathematical Assessment of Results of Investigation of the BCL-2 and Caspase-3 Protein Expression under the Influence of nanobiocomposites of Silver on natural and Synthetic Matrixes

2019 ◽  
Vol 3 (6) ◽  
pp. 150-155
Author(s):  
M. A. Novikov ◽  
E. A. Titov ◽  
V. A. Vokina ◽  
L. M. Sosedova
Keyword(s):  
Silver Nanoparticles ◽  
Discriminant Analysis ◽  
Caspase 3 ◽  
Early Period ◽  
Feature Space ◽  
Male Rats ◽  
Apoptotic Proteins ◽  
Comparison Of The Results ◽  
Two Stages

The aim was to assess the effect of silver nanoparticles on the expression of proand anti-apoptotic proteins caspase-3 and bcl-2 by discriminant analysis.Materials and methods. 120 sexually mature outbred male rats were divided into 8 groups (pure arabinogalactan (AG), pure poly-1-vinyl-1,2,4-triazol (PVT), nanobiocomposites on the AG and PVT matrix at a dose of 100 and 500 μg/kg. The administration was done orally for 9 days. The experimental study was carried out in two stages. The first stage included the examination of animals immediately after the end of the exposure of the studied substances (early period), the second stage – a survey 6 months after the end of the exposure (the long-term period).Results. The most distinguished groups were the groups that were administered silver nanoparticles on the AG matrix at a dose of 100 and 500 μg/kg.Conclusion. Comparison of the results of the discriminant analysis allowed to fully determine the effect of silver nanoparticles on the expression of proand anti-apoptotic proteins caspase-3 and bcl-2 when they were introduced on the AG and PVT matrix. In the AG groups and nanobiocomposites based on it, in the feature space, it was shown that the most remote by differentiating characteristics are the groups nAG100 and nAG500. In nanocomposites on the PVT matrix, a group of animals differing in differentiating features has not been identified.

scholarly journals Age-based immunomorphological analysis of rat testis in streptozotocin-induced diabetes mellitus

Pathologia ◽  
2021 ◽  
Vol 18 (1) ◽  
pp. 12-18
Author(s):  
I.-A. V. Kondrat ◽  
I. S. Shponka ◽  
T. V. Shynkarenko
Keyword(s):  
Diabetes Mellitus ◽  
Caspase 3 ◽  
Wilms Tumor ◽  
Age Groups ◽  
Early Period ◽  
Male Rats ◽  
Seminiferous Tubules ◽  
Public Healthcare ◽  
Adult Rats ◽  
Ki 67

Diabetes mellitus (DM) has emerged as a public healthcare problem. Sustained hyperglycemia has been linked with many complications including impaired male fertility. The aim of the study: to evaluate the effect of STZ-induced diabetes mellitus on testicular immunomorphology both in the peripubertal period and adulthood of rats. Material and methods. Peripubertal male rats were injected with STZ (70 mg/kg), adult rats received 60 mg/kg. Immunohistochemical staining was performed to assess cell proliferation (Ki-67), apoptosis (caspase-3), expression of androgen receptors (AR), Wilms Tumor (WT1) protein. Also, the morphology of blood vessels was analyzed on the basis of CD34-immunoreactivity. Taking into consideration the small groups of animals, statistical analysis was made with the Mann–Whitney U test. Results. Fewer rows of proliferating spermatogonia were observed in the experimental animals (P < 0.05) of both age groups. Surprisingly, diabetes resulted in decreased caspase-3 expression (P < 0.05) except for the early period (2 weeks) in the peripubertal group, in which this trend was not observed. The same principles are true in terms of AR expression in seminiferous tubules. Hyperglycemia prevented immature testes from the complete development but thickens the walls of microvessels (P < 0.05). Also, the atrophy of spermatogenic epithelium and Sertoli cells was registered in most tubules of all the experimental groups (P < 0.05). Conclusion. the diabetic injury of testicular tissue is a long time process possessing characteristic feature in the peripubertal period, for example, the later development of AR deficiency. In addition, the high level of apoptosis is characteristic of an immature testis and so is the tendency of caspase-immunoreactivity to persist.

scholarly journals Comparative Assessment of Silver Nanocomposites’ Biological Effects on the Natural and Synthetic Matrix

2021 ◽  
Vol 22 (24) ◽  
pp. 13257
Author(s):  
Mikhail A. Novikov ◽  
Eugeniy A. Titov ◽  
Larisa M. Sosedova ◽  
Viktor S. Rukavishnikov ◽  
Vera A. Vokina ◽  
...  
Keyword(s):  
Nervous Tissue ◽  
Biological Effects ◽  
Early Period ◽  
Comparative Assessment ◽  
Male Rats ◽  
Electron Microscopic ◽  
Temporal Area ◽  
Silver Nanocomposites ◽  
Natural And Synthetic

The aim of our investigation was to make a comparative assessment of the biological effects of silver nanoparticles encapsulated in a natural and synthetic polymer matrix. We carried out a comparative assessment of the biological effect of silver nanocomposites on natural (arabinogalactan) and synthetic (poly-1-vinyl-1,2,4-triazole) matrices. We used 144 three-month-old white outbred male rats, which were divided into six groups. Substances were administered orally for 9 days at a dose 500 μg/kg. Twelve rats from each group were withdrawn from the experiment immediately after nine days of exposure (early period), and the remaining 12 rats were withdrawn from the experiment 6 months after the end of the nine-day exposure (long-term period). We investigated the parietal–temporal area of the cerebral cortex using histological (morphological assessments of nervous tissue), electron microscopic (calculation of mitochondrial areas and assessment of the quality of the cell nucleus), and immunohistochemical methods (study of the expression of proteins regulating apoptosis bcl-2 and caspase 3). We found that the effect of the nanocomposite on the arabinogalactan matrix causes a disturbance in the nervous tissue structure, an increase in the area of mitochondria, a disturbance of the structure of nerve cells, and activation of the process of apoptosis.

scholarly journals Growth retardation induced by dexamethasone is associated with increased apoptosis of the growth plate chondrocytes

2003 ◽  
Vol 176 (3) ◽  
pp. 331-337 ◽  
Author(s):  
D Chrysis ◽  
EM Ritzen ◽  
L Savendahl
Keyword(s):  
Growth Plate ◽  
Growth Retardation ◽  
Caspase 3 ◽  
Male Rats ◽  
Apoptotic Cells ◽  
Growth Plate Chondrocytes ◽  
Glucocorticoid Treatment ◽  
Local Mechanism ◽  
Apoptotic Proteins ◽  
Multicellular Organisms

Glucocorticoids cause significant growth retardation in mammals and humans and decreased proliferation of chondrocytes has been considered as the main local mechanism. Death by apoptosis is an important regulator of homeostasis in multicellular organisms. Here we chose to study the role of apoptosis in growth retardation caused by glucocorticoid treatment. We treated 7-week-old male rats with dexamethasone (5 mg/kg/day) for 7 days. Apoptosis was studied in tibiae growth plates by the TUNEL method. Immunoreactivity for parathyroid hormone-related peptide (PTHrP), caspase-3, and the anti-apoptotic proteins Bcl-2 and Bcl-x was also studied. Apoptosis was mainly localized in terminal hypertropic chondrocytes (THCs) in both control and dexamethasone-treated animals. Dexamethasone caused an increase in apoptosis which was fourfold in THCs (2.45+/-0.12 vs 0.62+/-0.09 apoptotic cells/mm growth plate, P<0.001), and 18-fold in proliferative chondrocytes (0.18+/-0.04 vs 0.01+/-0.007 apoptotic cells/mm growth plate, P<0.001). Increased apoptosis after dexamethasone treatment was accompanied by increased immunoreactivity for caspase-3 and decreased immunoreactivity for the anti-apoptotic proteins Bcl-2 and Bcl-x, which further supports our apoptosis results. Dexamethasone also decreased the immunoreactivity for PTHrP, suggesting a role in the mechanism by which glucocorticoids induce apoptosis in the growth plate. We conclude that apoptosis is one mechanism involved in growth retardation induced by glucocorticoids. Premature loss of resting/proliferative chondrocytes by apoptosis could contribute to incomplete catch-up seen after prolonged glucocorticoid treatment.

scholarly journals Comparative Evaluation of the Expression of Apoptosis Proteins at the Influence of Metal Containing Polymeric Nanocomposites on the Brain

2019 ◽  
Vol 4 (2) ◽  
pp. 136-139
Author(s):  
M. A. Novikov ◽  
E. A. Titov
Keyword(s):  
Silver Nanoparticles ◽  
Safety Assessment ◽  
Caspase 3 ◽  
Siberian Larch ◽  
Male Rats ◽  
Careful Study ◽  
Main Function ◽  
Polymeric Nanocomposites ◽  
State Of Affairs ◽  
Apoptosis Proteins

Background. The creation and the attempt to introduce into medicine new nanostructured materials dictate the need for careful study of their safety for human health and the environment. At the same time, the existing classical approaches to safety assessment do not always objectively reflect the real state of affairs; therefore, there is a need to introduce new approaches, including methods of intracellular safety assessment of nanoparticles and nanomaterials.The aim of this study was to evaluate expression level of apoptosis-regular proteins caspase-3 and bcl-2 in neurons of white outbred rats exposed to nanocomposites consisting of nanoparticles of metals (silver, bismuth and gadolinium) on a natural matrix of arabinogalactan isolated from Siberian larch wood.Materials and methods. Sixty outbred male rats were exposed to nanocomposites for 9 days at a dose of 500 μg of metal per 1 kg of animal body weight. The introduction of substances was carried out orally using atraumatic probe. Study of the activity of proteins of apoptosis modulators caspase-3 and bcl-2 and calculate the percentage of different types of reacted neurons was conducted. Statistics 6.1 was used to process results, p-values less than 0.01 were acknowledged as statistically significant.Results. It was found that the activation of apoptotic process occurs only when the nanocomposite is affected with the addition of silver nanoparticles, which are realized in a significant increase in the percentage of all types of neurons under study.Conclusion. Monitoring proteins – regulators of apoptosis can serve as a marker of early damage to nerve cells, since glial cells can form at the site of dead neurons that do not fulfill the main function of transmission of nerve impulses. The results obtained allow us to substantiate the need for a more thorough and prolonged study of silver nanoparticles encapsulated on the arabinogalactan matrix.

The effect of dexamethasone on mucosal immunity of uterine tissue during pregnancy in rat

2019 ◽  
Vol 20 (1) ◽  
pp. 75-84
Keyword(s):  
Early Pregnancy ◽  
Histopathological Examination ◽  
Early Period ◽  
Interleukin 10 ◽  
Treated Group ◽  
Female Rats ◽  
Male Rats ◽  
Mrna Levels ◽  
Uterine Tissue ◽  
Leukocytic Infiltration

Disturbances in early pregnancy immunity affect embryo development, endometrial receptivity, placental development, fetal growth and lead to subfertility, dexamethasone is a synthetic glucocorticoid used for treatment of various complications. Immune cells and cytokines were examined during the early pregnancy in twenty-four female rats and six male rats for mating. Rats were grouped into two group control and dexamethasone treated by a dose of 50µgm/kgm body weight daily starting from one week before mating and persisted for one week after pregnancy. Blood samples were collected from each rat at 5hrs and at 1,3,7 day of pregnancy. Extracted RNA was subjected to real time PCR to determine mRNA levels for immune related genes interleukin1a(IL1A) and interleukin 10(IL10). Histopathological examination was done to uterus in order to detect leukocyte infiltration in uterine tissue. Results showed that significant increase in white blood cell count mainly eosinophil at 5hrs and lymphocyte at three and seven day of pregnancy of dexamethasone treated group. Moreover, TNF, C-reactive protein and progesterone were increased mainly at seven day of pregnancy of dexamethasone treated group. Similarly, interleukin 1alpha and interleukin 10 significantly increased at 5hrs and one day of pregnancy of dexamethasone treated group. In contrast, serum levels of total antioxidant capacity and estrogen were decreased significantly at 5hrs and seven day in dexamethasone treated group. Histopathological examination of uterus revealed leukocytic infiltration especially neutrophil and few eosinophils at five hours and one day of gestation then eosinophil become absent at 3day and seven day of dexamethasone group. Epithelial height and uterine gland diameter significantly increased at 5hrs, three day and seven days of gestation of dexamethasone treated group. The present investigation demonstrated that using of dexamethasone by dose of 50µgm/kgm during early pregnancy had a conflicting impact on some immune cytokines and parameters and may reflect a harmful response of immune system toward early period of pregnancy

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