scholarly journals High accuracy of quantitative fluorescence polymerase chain reaction combined with non‑invasive pre‑natal testing for mid‑pregnancy diagnosis of common fetal aneuploidies: A single‑center experience in China

2019 ◽  
Author(s):  
Ping Huo ◽  
Qiuyan Luo ◽  
Juan Li ◽  
Baoquan Jiao ◽  
Limin Rong ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
High Accuracy ◽  
Single Center ◽  
Chain Reaction ◽  
Pregnancy Diagnosis ◽  
Non Invasive ◽  
Single Center Experience ◽  
Polymerase Chain ◽  
Fetal Aneuploidies ◽  
Quantitative Fluorescence

scholarly journals RT-qPCR Testing of SARS-CoV-2: A Primer

2020 ◽  
Vol 21 (8) ◽  
pp. 3004 ◽  
Author(s):  
Stephen A. Bustin ◽  
Tania Nolan
Keyword(s):  
Polymerase Chain Reaction ◽  
Reverse Transcription ◽  
Diagnostic Tool ◽  
Acute Infection ◽  
Chain Reaction ◽  
Reliable Test ◽  
Polymerase Chain ◽  
Quantitative Fluorescence

Testing for the presence of coronavirus is an essential diagnostic tool for monitoring and managing the current COVID-19 pandemic. The only reliable test in current use for testing acute infection targets the genome of SARS-CoV-2, and the most widely used method is quantitative fluorescence-based reverse transcription polymerase chain reaction (RT-qPCR). Despite its ubiquity, there is a significant amount of uncertainty about how this test works, potential throughput and reliability. This has resulted in widespread misrepresentation of the problems faced using this test during the current COVID-19 epidemic. This primer provides simple, straightforward and impartial information about RT-qPCR.

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