Donor-transmitted malignancy confirmed by quantitative fluorescence polymerase chain reaction genotype analysis: A rare indication for liver retransplantation

2008 ◽  
Vol 14 (2) ◽  
pp. 155-158 ◽  
Author(s):  
Katie Snape ◽  
Louise Izatt ◽  
Paul Ross ◽  
David Ellis ◽  
Kathy Mann ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Genotype Analysis ◽  
Polymerase Chain ◽  
Liver Retransplantation ◽  
Quantitative Fluorescence

scholarly journals RT-qPCR Testing of SARS-CoV-2: A Primer

2020 ◽  
Vol 21 (8) ◽  
pp. 3004 ◽  
Author(s):  
Stephen A. Bustin ◽  
Tania Nolan
Keyword(s):  
Polymerase Chain Reaction ◽  
Reverse Transcription ◽  
Diagnostic Tool ◽  
Acute Infection ◽  
Chain Reaction ◽  
Reliable Test ◽  
Polymerase Chain ◽  
Quantitative Fluorescence

Testing for the presence of coronavirus is an essential diagnostic tool for monitoring and managing the current COVID-19 pandemic. The only reliable test in current use for testing acute infection targets the genome of SARS-CoV-2, and the most widely used method is quantitative fluorescence-based reverse transcription polymerase chain reaction (RT-qPCR). Despite its ubiquity, there is a significant amount of uncertainty about how this test works, potential throughput and reliability. This has resulted in widespread misrepresentation of the problems faced using this test during the current COVID-19 epidemic. This primer provides simple, straightforward and impartial information about RT-qPCR.

Quantitative fluorescence polymerase chain reaction for the rapid prenatal detection of common aneuploidies and fetal sex

1997 ◽  
Vol 177 (4) ◽  
pp. 899-906 ◽  
Author(s):  
Barbara Pertl ◽  
Susanne Kopp a ◽  
Peter M. Kroisel ◽  
Martin Häusler ◽  
Jon Sherlock ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Fetal Sex ◽  
Prenatal Detection ◽  
Polymerase Chain ◽  
Quantitative Fluorescence

scholarly journals Frequency of Chromosomal Abnormalities in Products of Conception

2017 ◽  
Vol 39 (03) ◽  
pp. 110-114 ◽  
Author(s):  
Thaís Teles ◽  
Carolina Paula ◽  
Mariana Ramos ◽  
Helena Costa ◽  
Cyntia Andrade ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Chromosomal Aberrations ◽  
Maternal Age ◽  
Chromosomal Abnormalities ◽  
Chain Reaction ◽  
Products Of Conception ◽  
Polymerase Chain ◽  
Conventional Cytogenetics ◽  
Quantitative Fluorescence ◽  
Abnormal Results

Purpose To describe the frequencies of chromosomal abnormalities found in abortion material, and to observe its correlation to maternal age. Methods A retrospective study was conducted based on data obtained from the databank of a medical genetics laboratory in Belo Horizonte, MG, Brazil. A total of 884 results from products of conception analysis were included, 204 of which were analyzed by cytogenetics, and 680 by molecular biology based on quantitative fluorescence polymerase chain reaction (QF-PCR). The frequency of individual chromosomal aberrations and the relationship between the presence of anomalies and maternal age were also evaluated. Results The conventional cytogenetics technique was able to detect 52% of normal and 48% of abnormal results in the analyzed material. Quantitative fluorescence polymerase chain reaction revealed 60% of normal and 40% of abnormal results from the samples evaluated by this method. The presence of trisomy 15 was detected only by cytogenetics, as it was not included in the QF-PCR routine investigation in the laboratory. A significant increase in abnormal results was observed among women aged 35 years or older compared with younger women (p = 0.02). Conclusion Chromosomal aberrations are still a major cause of spontaneous abortion, and the conventional cytogenetics technique is efficient for miscarriage material analysis, but molecular methods such as QF-PCR are adequate complementary strategies to detect the major chromosomal anomalies, leading to technical reports with reliable results.

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