scholarly journals Impact of gabapentin on neuronal high voltage-activated Ca2+ channel properties of injured-side axotomized and adjacent uninjured dorsal root ganglions in a rat model of spinal nerve ligation

2017 ◽  
Vol 13 (3) ◽  
pp. 851-860 ◽  
Author(s):  
Minmin Zhu ◽  
Xiaodi Sun ◽  
Xiaodong Chen ◽  
Hang Xiao ◽  
Manlin Duan ◽  
...  
Keyword(s):  
High Voltage ◽  
Rat Model ◽  
Dorsal Root ◽  
Spinal Nerve ◽  
Spinal Nerve Ligation ◽  
Dorsal Root Ganglions ◽  
Injured Side ◽  
Channel Properties

scholarly journals Biomarker Analysis of Orally Dosed, Dual Active, Matrix Metalloproteinase (MMP)-2 and MMP-9 Inhibitor, AQU-118, in the Spinal Nerve Ligation (SNL) Rat Model of Neuropathic Pain

2019 ◽  
Vol 20 (4) ◽  
pp. 811 ◽  
Author(s):  
Mei Kwan ◽  
Anthony Choo ◽  
Taleen Hanania ◽  
Afshin Ghavami ◽  
Jose Beltran ◽  
...  
Keyword(s):  
Neuropathic Pain ◽  
Dorsal Root Ganglion ◽  
Rat Model ◽  
Dorsal Root ◽  
Mechanical Allodynia ◽  
Caspase 3 ◽  
Spinal Nerve ◽  
Spinal Nerve Ligation ◽  
Oral Dosing ◽  
Protein Levels

There is an unmet medical need for the development of non-addicting pain therapeutics with enhanced efficacy and tolerability. The current study examined the effects of AQU-118, an orally active inhibitor of metalloproteinase-2 (MMP-2) and MMP-9, in the spinal nerve ligation (SNL) rat model of neuropathic pain. Mechanical allodynia and the levels of various biomarkers were examined within the dorsal root ganglion (DRG) before and after oral dosing with AQU-118. The rats that received the SNL surgery exhibited significant mechanical allodynia as compared to sham controls. Animals received either vehicle, positive control (gabapentin), or AQU-118. After SNL surgery, the dorsal root ganglion (DRG) of those rats dosed with vehicle had elevated messenger RNA (mRNA) expression levels for MMP-2, IL1-β & IL-6 and elevated protein levels for caspase-3 while exhibiting decreased protein levels for myelin basic protein (MBP) & active IL-β as compared to sham controls. Rats orally dosed with AQU-118 exhibited significantly reduced mechanical allodynia and decreased levels of caspase-3 in the DRG as compared to vehicle controls. Results demonstrate that oral dosing with the dual active, MMP-2/-9 inhibitor, AQU-118, attenuated mechanical allodynia while at the same time significantly reduced the levels of caspase-3 in the DRG.

scholarly journals Three-dimensional analysis of the characteristics of joint motion and gait pattern in a rodent model following spinal nerve ligation

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Takayuki Seto ◽  
Hidenori Suzuki ◽  
Tomoya Okazaki ◽  
Yasuaki Imajo ◽  
Norihiro Nishida ◽  
...  
Keyword(s):  
Neuropathic Pain ◽  
Pain Intensity ◽  
Rat Model ◽  
Gait Pattern ◽  
Spinal Nerve ◽  
Spinal Nerve Ligation ◽  
Joint Motion ◽  
Behavioral Reactions ◽  
Post Operation ◽  
The Right

Abstract Background The spinal nerve ligation (SNL) rat is well known as the most common rodent model of neuropathic pain without motor deficit. Researchers have performed analyses using only the von Frey and thermal withdrawal tests to evaluate pain intensity in the rat experimental model. However, these test are completely different from the neurological examinations performed clinically. We think that several behavioral reactions must be observed following SNL because the patients with neuropathic pain usually have impaired coordination of the motions of the right–left limbs and right–left joint motion differences. In this study, we attempted to clarify the pain behavioral reactions in SNL rat model as in patients. We used the Kinema-Tracer system for 3D kinematics gait analysis to identify new characteristic parameters of each joint movement and gait pattern. Results The effect of SNL on mechanical allodynia was a 47 ± 6.1% decrease in the withdrawal threshold during 1–8 weeks post-operation. Sagittal trajectories of the hip, knee and ankle markers in SNL rats showed a large sagittal fluctuation of each joint while walking. Top minus bottom height of the left hip and knee that represents instability during walking was significantly larger in the SNL than sham rats. Both-foot contact time, which is one of the gait characteristics, was significantly longer in the SNL versus sham rats: 1.9 ± 0.15 s vs. 1.03 ± 0.15 s at 4 weeks post-operation (p = 0.003). We also examined the circular phase time to evaluate coordination of the right and left hind-limbs. The ratio of the right/left circular time was 1.0 ± 0.08 in the sham rats and 0.62 ± 0.15 in the SNL rats at 4 weeks post-operation. Conclusions We revealed new quantitative parameters in an SNL rat model that are directly relevant to the neurological symptoms in patients with neuropathic pain, in whom the von Frey and thermal withdrawal tests are not used at all clinically. This new 3D analysis system can contribute to the analysis of pain intensity of SNL rats in detail similar to human patients’ reactions following neuropathic pain.

scholarly journals Optogenetic stimulation in motor cortex and PKCγ knockdown at dorsal root ganglions alter hyperalgesia behaviors in chronic compressed DRG pain rat model

IBRO Reports ◽  
2019 ◽  
Vol 6 ◽  
pp. S348
Author(s):  
Jaisan Islam ◽  
Elina Kc ◽  
Hyeong Cheol Moon ◽  
Kyoung Ha So ◽  
Sang Hwan Hyun ◽  
...  
Keyword(s):  
Motor Cortex ◽  
Rat Model ◽  
Dorsal Root ◽  
Dorsal Root Ganglions ◽  
Optogenetic Stimulation

Effects of selective spinal nerve ligation on acetic acid-induced nociceptive responses and ASIC3 immunoreactivity in the rat dorsal root ganglion

2008 ◽  
Vol 1219 ◽  
pp. 26-31 ◽  
Author(s):  
Megumi Omori ◽  
Masataka Yokoyama ◽  
Yoshikazu Matsuoka ◽  
Hiroyuki Kobayashi ◽  
Satoshi Mizobuchi ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Dorsal Root Ganglion ◽  
Dorsal Root ◽  
Spinal Nerve ◽  
Spinal Nerve Ligation ◽  
Nociceptive Responses

Differential expression of alpha1-adrenoceptor subtype mRNAs in the dorsal root ganglion after spinal nerve ligation

2001 ◽  
Vol 93 (2) ◽  
pp. 164-172 ◽  
Author(s):  
Jiangang Xie ◽  
Young Ho Lee ◽  
Chen Wang ◽  
Jin Mo Chung ◽  
Kyungsoon Chung
Keyword(s):  
Dorsal Root Ganglion ◽  
Differential Expression ◽  
Dorsal Root ◽  
Spinal Nerve ◽  
Spinal Nerve Ligation

Re-evaluation of the phenotypic changes in L4 dorsal root ganglion neurons after L5 spinal nerve ligation

Pain ◽  
2012 ◽  
Vol 153 (1) ◽  
pp. 68-79 ◽  
Author(s):  
Tetsuo Fukuoka ◽  
Hiroki Yamanaka ◽  
Kimiko Kobayashi ◽  
Masamichi Okubo ◽  
Kan Miyoshi ◽  
...  
Keyword(s):  
Dorsal Root Ganglion ◽  
Dorsal Root ◽  
Spinal Nerve ◽  
Spinal Nerve Ligation ◽  
Dorsal Root Ganglion Neurons ◽  
Phenotypic Changes ◽  
Ganglion Neurons
Sign in / Sign up

Export Citation Format

Share Document