scholarly journals Epigallocatechin-3-gallate inhibits migration and invasion of human renal carcinoma cells by downregulating matrix metalloproteinase-2 and matrix metalloproteinase-9

2016 ◽  
Vol 11 (4) ◽  
pp. 1243-1248 ◽  
Author(s):  
SHAO-JUN CHEN ◽  
XU-DONG YAO ◽  
BO PENG ◽  
YUN-FEI XU ◽  
GUANG-CHUN WANG ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Renal Carcinoma ◽  
Matrix Metalloproteinase 9 ◽  
Carcinoma Cells ◽  
Matrix Metalloproteinase 2 ◽  
Migration And Invasion ◽  
Metalloproteinase 9 ◽  
Human Renal Carcinoma ◽  
Renal Carcinoma Cells

scholarly journals Alpha-Mangostin Suppresses the Metastasis of Human Renal Carcinoma Cells by Targeting MEK/ERK Expression and MMP-9 Transcription Activity

2017 ◽  
Vol 44 (4) ◽  
pp. 1460-1470 ◽  
Author(s):  
Chien-Min Chen ◽  
Shu-Ching Hsieh ◽  
Chia-Liang Lin ◽  
Yu-Syun Lin ◽  
Jen-Pi Tsai ◽  
...  
Keyword(s):  
Cell Migration ◽  
Renal Carcinoma ◽  
Carcinoma Cells ◽  
Luciferase Assay ◽  
Migration And Invasion ◽  
Mrna Levels ◽  
Dependent Manner ◽  
Cell Migration And Invasion ◽  
Human Renal Carcinoma ◽  
Renal Carcinoma Cells

Background/Aims: α-mangostin has anti-carcinogenic effects against several cancers. We investigated the molecular mechanism of this compound on the metastasis of human renal carcinoma cells. Methods: Cell viability was measured using the MTT assay, and cell cycle distribution using flow cytometry. A Matrigel-based assay was used to measure in vitro cell migration and invasion. MAPK-related proteins and matrix metalloproteinase (MMP)-9 and MMP-2 expression were measured by western blotting, and MMP2/-9 activities were determined by gelatin zymography. RT-qPCR and a luciferase assay were used to examine the transcriptional activity of MMP-9. Results: α-mangostin inhibited the migration and invasion of RCC cells in a dose-dependent manner, but had no evident cytotoxic effects. Treatment of 786-O cells with α-mangostin inhibited activation of MEK and ERK. Treatment with a specific MEK inhibitor (U0126) enhanced the inhibitory effects of α-mangostin on cell migration and invasion, and the phosphorylation of ERK and MEK. Moreover, α-mangostin inhibited the expression of the MMP-9 mRNA levels as well as the activity of MMP-9 promoter, and these suppressive effects were further enhanced by U0126. Conclusions: Our results suggest that α-mangostin suppresses cell migration and invasion via MEK/ERK/MMP9 pathway, and might be a promising anti-metastatic agent against human renal cell carcinoma.

Tianeptine sodium salt suppresses TNF-α-induced expression of matrix metalloproteinase-9 in human carcinoma cells via suppression of the PI3K/Akt-mediated NF-κB pathway

2014 ◽  
Vol 38 (2) ◽  
pp. 502-509 ◽  
Author(s):  
Rajapaksha Gedara Prasad Tharanga Jayasooriya ◽  
Matharage Gayani Dilshara ◽  
Yung Hyun Choi ◽  
Sung-Kwon Moon ◽  
Wun-Jae Kim ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Sodium Salt ◽  
Matrix Metalloproteinase 9 ◽  
Carcinoma Cells ◽  
Induced Expression ◽  
Human Carcinoma ◽  
Tnf Α ◽  
Metalloproteinase 9
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