scholarly journals Hydrogen-rich saline attenuates steroid-associated femoral head necrosis through inhibition of oxidative stress in a rabbit model

2015 ◽  
Vol 11 (1) ◽  
pp. 177-182 ◽  
Author(s):  
SHENG-LI HUANG ◽  
JIAN JIAO ◽  
HONG-WEI YAN
Keyword(s):  
Oxidative Stress ◽  
Femoral Head ◽  
Rabbit Model ◽  
Femoral Head Necrosis

scholarly journals Early detection of steroid-induced femoral head necrosis using 99mTc-Cys-Annexin V-based apoptosis imaging in a rabbit model

2020 ◽  
Vol 26 (1) ◽  
Author(s):  
Xiaolong Wang ◽  
Jianbo Li ◽  
Da Man ◽  
Rui Liu ◽  
Jianmin Zhao
Keyword(s):  
Femoral Head ◽  
Photon Emission ◽  
Rabbit Model ◽  
Annexin V ◽  
Spect Imaging ◽  
Femoral Head Necrosis ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Control Group ◽  
Emission Computed Tomography ◽  
Target Tissue

Abstract Background At present, the early diagnosis of femoral head necrosis mainly relies on Magnetic resonance imaging (MRI), and most early patients are difficult to make an accurate diagnosis. Therefore, to investigate the early diagnostic value of 99mTc-Cys-Annexin V Single-photon emission computed tomography (SPECT) imaging were compared with MRI in rabbit models of steroid-induced femoral head necrosis. Methods The animal model of steroid-induced femoral head necrosis (SIFHN) was established in 5-month-old healthy New Zealand white rabbits by injecting horse serum into ear vein and methylprednisolone into gluteal muscle, the purpose of modeling is to simulate the actual clinical situation of SIFNH. 99mTc-Cys-Annexin V SPECT imaging and MRI were performed at 2nd week, 4th week, and 6th week after modeling. After that, histopathology was used to verify the success of modeling. Apoptosis was detected by transmission electron microscopy (TEM) and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling assay (TUNEL). Results At 2 weeks after the injection of hormone, 99mTc-Cys-Annexin V SPECT image showed abnormal radioactive uptake in the bilateral femoral head. And over time, the radioactivity concentration was more obvious, and the ratio of T/NT (target tissue/non-target tissues, which is the ratio of femoral head and the ipsilateral femoral shaft) was gradually increased. In the 99mTc-Cys-Annexin V SPECT imaging at each time point, T/NT ratio of the model group was significantly higher than that of the control group (P < 0.01); at 4 weeks after the injection of hormone, MRI showed an abnormal signal of osteonecrosis. At 2, 4, and 6 weeks after hormone injection, apoptosis was observed by TUNEL and TEM. Conclusions 99mTc-Cys-Annexin V SPECT imaging can diagnose steroid-induced femoral head necrosis earlier than MRI, and has potential application value for non-invasively detecting early and even ultra-early stage of femoral head necrosis.

scholarly journals Early Detection of Steroid-Induced Femoral Head Necrosis using 99mTc-Cys-Annexin V-based Apoptosis Imaging in a Rabbit Model

2020 ◽  
Author(s):  
Xiaolong Wang ◽  
Jianbo Li ◽  
Da Man ◽  
Rui Liu ◽  
Jianmin Zhao
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Femoral Head ◽  
Rabbit Model ◽  
Annexin V ◽  
Spect Imaging ◽  
Femoral Head Necrosis ◽  
Control Group ◽  
Target Tissue ◽  
Transmission Electron

Abstract Background At present, the early diagnosis of femoral head necrosis mainly relies on MRI, and most early patients are difficult to make an accurate diagnosis. Therefore, to investigate the early diagnostic value of 99mTc-Cys-Annexin V SPECT imaging were compared with MRI in rabbit models of steroid-induced femoral head necrosis. Methods The rabbit models of steroid-induced femoral head necrosis were established by intravenous injection of horse serum and gluteal muscle injection of methylprednisolone in of 5-month-old healthy New Zealand white rabbits. 99mTc-Cys-Annexin V SPECT imaging and MRI were performed at 2nd week, 4th week, and 6th week after modeling. After that, histopathology was used to verify the success of modeling. Apoptosis was detected by transmission electron microscopy and TUNEL. Results At 2 weeks after the injection of hormone, 99mTc-Cys-Annexin V SPECT image showed abnormal radioactive uptake in the bilateral femoral head. And over time, the radioactivity concentration was more obvious, and the ratio of T/NT (target tissue/non-target tissues) was gradually increased. In the SPECT imaging at each time point, T/NT ratio of the model group was significantly higher than that of the control group (P < 0.01); at 4 weeks after the injection of hormone, MRI showed an abnormal signal of osteonecrosis. At 2, 4, and 6 weeks after hormone injection, apoptosis was observed by TUNEL and transmission electron microscopy. Conclusions 99mTc-Cys-Annexin V SPECT imaging can diagnose steroid-induced femoral head necrosis earlier than MRI, and has potential application value for non-invasively detecting early and even ultra-early stage of femoral head necrosis.

scholarly journals ID: 1071 Establishing an early-stage femoral head necrosis model of rabbit using methylprednisolone and Complete Freund's Adjuvant

2017 ◽  
Vol 4 (S) ◽  
pp. 153
Author(s):  
Lan Thi Phi ◽  
Thuy Thi-Thanh Dao ◽  
Khanh Dinh-Van Nguyen ◽  
Phuc Van Pham ◽  
Khanh Hong-Thien Bui ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Bone Marrow ◽  
Body Weight ◽  
Femoral Head ◽  
Early Stage ◽  
Rabbit Model ◽  
Femoral Head Necrosis ◽  
Group A ◽  
Freund's Adjuvant ◽  
Group B

Background: Pathological animal models provide the foundation for developing new methods for treating. This research aims to establish a rabbit model of femoral head necrosis. Osteonecrosis of the femoral head (ONFH) was induced in rabbits by using methylpresnisolone combined with Complete Freund's Adjuvant (CFA). New Zealand White rabbits were divided into two groups. Group A (n=10) was given an intramuscular injection of 0.5 mg/kg (CFA) and 40mg/kg methylprednisolone (MPS). Group B (n=6) was received normal saline at the same location and same volume as those in Group A. The efficiency of ONFH rabbit model was assessed at 7 weeks after the last injection. Body weight was weighed. The histological structure of head femoral and bone were deteded by H&E staining. The empty lacuna was counted. Cartilage degeneration was evaluated using image analysis software. Blood vessel density was assessed after ink artery infusion. The cell cycle of bone marrow-derived mononuclear cells was analyzed by flow cytometry. The results showed that there was no difference in body weight change of rabbits between two groups. However, the bone morphology and cartilage surface of femoral head were abnormalities at group A. The percentage of empty osteocyte lacunae were significantly higher in Group A than Group B. Chondrocyte degeneration and fibrocartilage expression were observed at Group A. Compare to group B, Group A had less ink-stained blood vessels. Moreover, the fraction of bone marrow-derived mononuclear at S phase and G2/M phase of the cell cycle was significantly decreased in group A. Thus, CFA combined with MPS can  be used to establishing an early-stage femoral head necrosis model of rabbit.

scholarly journals Development of an early-stage femoral head necrosis rabbit model using methylprednisolone and Complete Freund's Adjuvant

2017 ◽  
Vol 4 (11) ◽  
pp. 1749
Author(s):  
Lan Thi Phi ◽  
Ha Thi -Ngan Le ◽  
Thuy Thi -Thanh Dao ◽  
Khanh Dinh-Van Nguyen ◽  
Khanh Hong-Thien Bui ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Bone Marrow ◽  
Body Weight ◽  
Femoral Head ◽  
Mononuclear Cells ◽  
Early Stage ◽  
Rabbit Model ◽  
Normal Group ◽  
Femoral Head Necrosis ◽  
Freund's Adjuvant

Introduction: Pathological animal models provide the foundation for developing new methods for disease treatment. This research aims to establish a rabbit model of femoral head necrosis. Methods: Osteonecrosis of the femoral head (ONFH) was induced in rabbits by using methylprednisolone (MPS) combined with Complete Freund's Adjuvant (CFA). New Zealand White rabbits were divided into two groups. ONFH group (n=10) was given an intramuscular injection of 0.5 mg/kg CFA and 40 mg/kg methylprednisolone. Normal group (n=6) received normal saline at the same location and same volume as those in ONFH group. The efficiency of the ONFH rabbit model was assessed at week 7 after the last injection. Body weight was detected, and the histological structure of head femoral and bone were assessed by H&E staining. The empty lacunae were counted. Cartilage degeneration was evaluated using image analysis software. Blood vessel density was assessed after ink artery infusion. The cell cycle of bone marrow-derived mononuclear cells was analyzed by flow cytometry. Results: The results showed that there was no difference in body weight changes of rabbits between the two groups. However, the bone morphology and cartilage surface of the femoral head showed abnormalities in the ONFH group. The percentage of empty osteocyte lacunae was significantly higher in ONFH group than normal group. Chondrocyte degeneration and fibrocartilage expression were observed in the ONFH group. Compared to the normal group, the ONFH group had less ink-stained blood vessels. However, the fraction of bone marrow-derived mononuclear cells in S phase and G2/M phase of the cell cycle was significantly increased in the ONFH group. Conclusion: Thus, CFA combined with MPS for 7 weeks can be used to establish an early-stage femoral head necrosis model in rabbits.

scholarly journals Oxidative Stress Induced Osteocyte Apoptosis in Steroid‐Induced Femoral Head Necrosis

2021 ◽  
Author(s):  
Zhen‐Qi Fan ◽  
Shu‐Cai Bai ◽  
Qian Xu ◽  
Zhi‐Jun Li ◽  
Wen‐Hao Cui ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Femoral Head ◽  
Femoral Head Necrosis ◽  
Osteocyte Apoptosis

scholarly journals Early Detection of Steroid-Induced Femoral Head Necrosis using 99mTc-Cys-Annexin V-based Apoptosis Imaging in a Rabbit Model

2020 ◽  
Author(s):  
Xiaolong Wang ◽  
Jianbo Li ◽  
Da Man ◽  
Rui Liu ◽  
Jianmin Zhao
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Femoral Head ◽  
Rabbit Model ◽  
Annexin V ◽  
Spect Imaging ◽  
Femoral Head Necrosis ◽  
Control Group ◽  
Target Tissue ◽  
Transmission Electron

Abstract Background At present, the early diagnosis of femoral head necrosis mainly relies on MRI, and most early patients are difficult to make an accurate diagnosis. Therefore, to investigate the early diagnostic value of 99mTc-Cys-Annexin V SPECT imaging were compared with MRI in rabbit models of steroid-induced femoral head necrosis. Methods The rabbit models of steroid-induced femoral head necrosis were established by intravenous injection of horse serum and gluteal muscle injection of methylprednisolone in of 5-month-old healthy New Zealand white rabbits. 99mTc-Cys-Annexin V SPECT imaging and MRI were performed at 2nd week, 4th week, and 6th week after modeling. After that, histopathology was used to verify the success of modeling. Apoptosis was detected by transmission electron microscopy and TUNEL. Results At 2 weeks after the injection of hormone, 99mTc-Cys-Annexin V SPECT image showed abnormal radioactive uptake in the bilateral femoral head. And over time, the radioactivity concentration was more obvious, and the ratio of T/NT (target tissue/non-target tissues) was gradually increased. In the SPECT imaging at each time point, T/NT ratio of the model group was significantly higher than that of the control group (P < 0.01); at 4 weeks after the injection of hormone, MRI showed an abnormal signal of osteonecrosis. At 2, 4, and 6 weeks after hormone injection, apoptosis was observed by TUNEL and transmission electron microscopy. Conclusion s 99mTc-Cys-Annexin V SPECT imaging can diagnose steroid-induced femoral head necrosis earlier than MRI, and has potential application value for non-invasively detecting early and even ultra-early stage of femoral head necrosis.

scholarly journals Early Detection of Steroid-Induced Femoral Head Necrosis using 99mTc-Cys-Annexin V-based Apoptosis Imaging in a Rabbit Model

2020 ◽  
Author(s):  
Xiaolong Wang ◽  
Jianbo Li ◽  
Da Man ◽  
Rui Liu ◽  
Jianmin Zhao
Keyword(s):  
Femoral Head ◽  
Photon Emission ◽  
Rabbit Model ◽  
Annexin V ◽  
Spect Imaging ◽  
Femoral Head Necrosis ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Control Group ◽  
Emission Computed Tomography ◽  
Target Tissue

Abstract BackgroundAt present, the early diagnosis of femoral head necrosis mainly relies on Magnetic resonance imaging (MRI), and most early patients are difficult to make an accurate diagnosis. Therefore, to investigate the early diagnostic value of 99mTc-Cys-Annexin V Single-photon emission computed tomography (SPECT) imaging were compared with MRI in rabbit models of steroid-induced femoral head necrosis.MethodsThe animal model of steroid-induced femoral head necrosis (SIFHN) was established in 5-month-old healthy New Zealand white rabbits by injecting horse serum into ear vein and methylprednisolone into gluteal muscle, the purpose of modeling is to simulate the actual clinical situation of SIFNH. 99mTc-Cys-Annexin V SPECT imaging and MRI were performed at 2nd week, 4th week, and 6th week after modeling. After that, histopathology was used to verify the success of modeling. Apoptosis was detected by transmission electron microscopy (TEM) and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling assay (TUNEL). ResultsAt 2 weeks after the injection of hormone, 99mTc-Cys-Annexin V SPECT image showed abnormal radioactive uptake in the bilateral femoral head. And over time, the radioactivity concentration was more obvious, and the ratio of T/NT (target tissue/non-target tissues, which is the ratio of femoral head and the ipsilateral femoral shaft) was gradually increased. In the 99mTc-Cys-Annexin V SPECT imaging at each time point, T/NT ratio of the model group was significantly higher than that of the control group (P < 0.01); at 4 weeks after the injection of hormone, MRI showed an abnormal signal of osteonecrosis. At 2, 4, and 6 weeks after hormone injection, apoptosis was observed by TUNEL and TEM. Conclusions99mTc-Cys-Annexin V SPECT imaging can diagnose steroid-induced femoral head necrosis earlier than MRI, and has potential application value for non-invasively detecting early and even ultra-early stage of femoral head necrosis.

Review on hyperbaric oxygen treatment in femoral head necrosis

2017 ◽  
Vol 44 (6) ◽  
pp. 497-508 ◽  
Author(s):  
Enrico Camporesi ◽  
◽  
Giuliano Vezzani ◽  
Vincenzo Zanon ◽  
Daniele Manelli ◽  
...  
Keyword(s):  
Femoral Head ◽  
Hyperbaric Oxygen ◽  
Femoral Head Necrosis ◽  
Hyperbaric Oxygen Treatment ◽  
Oxygen Treatment
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