scholarly journals Atrial natriuretic peptide reduces the α-subunit of the epithelial sodium channel mRNA expression in the mouse stria vascularis

2014 ◽  
Vol 3 (2) ◽  
pp. 159-162 ◽  
Author(s):  
YAN LUO ◽  
QINGJIE XIA ◽  
ZENGLIANG XIA ◽  
YUEDI TANG
Keyword(s):  
Atrial Natriuretic Peptide ◽  
Sodium Channel ◽  
Mrna Expression ◽  
Natriuretic Peptide ◽  
Stria Vascularis ◽  
Epithelial Sodium Channel ◽  
Α Subunit ◽  
Atrial Natriuretic

scholarly journals Atrial natriuretic peptide modulates cystic fibrosis transmembrane conductance regulator chloride channel expression in rat proximal colon and human intestinal epithelial cells

2006 ◽  
Vol 189 (1) ◽  
pp. 155-165 ◽  
Author(s):  
H J Novaira ◽  
D S Ornellas ◽  
T M Ortiga-Carvalho ◽  
X M Zhang ◽  
J Souza-Menezes ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Epithelial Cells ◽  
Atrial Natriuretic Peptide ◽  
Mrna Expression ◽  
Natriuretic Peptide ◽  
Proximal Colon ◽  
Transmembrane Conductance Regulator ◽  
Transmembrane Conductance ◽  
Cystic Fibrosis Transmembrane ◽  
Atrial Natriuretic

The cystic fibrosis transmembrane conductance regulator (CFTR) is one of the most intensively investigated Cl− channels. Different mutations in the CFTR gene cause the disease cystic fibrosis (CF). CFTR is expressed in the apical membrane of various epithelial cells including the intestine. The major organ affected in CF patients is the lung, but it also causes an important dysfunction of intestinal ion transport. The modulation of CFTR mRNA expression by atrial natriuretic peptide (ANP) was investigated in rat proximal colon and in human intestinal CaCo-2 cells by RNase protection assay and semi-quantitative reverse transcriptase PCR techniques. Groups of rats subjected to volume expansion or intravenous infusion of synthetic ANP showed respective increases of 60 and 50% of CFTR mRNA expression in proximal colon. CFTR mRNA was also increased in cells treated with ANP, reaching a maximum effect at 10−9 M ANP, probably via cGMP. ANP at 10−9 M was also able to stimulate both the CFTR promoter region (by luciferase assay) and protein expression in CaCo-2 cells (by Western blot and immunoprecipitation/phosphorylation). These results suggested the involvement of ANP, a hormone involved with extracellular volume, in the expression of CFTR in rat proximal colon and CaCo-2 intestinal cells.

Changes in atrial natriuretic peptide concentration and expression of its receptors after pneumonectomy in the rat

2000 ◽  
Vol 99 (4) ◽  
pp. 343-348 ◽  
Author(s):  
Kohichi TAMURA ◽  
Shinzo TAKAMORI ◽  
Hiroharu MIFUNE ◽  
Akihiro HAYASHI ◽  
Kazuo SHIROUZU
Keyword(s):  
Atrial Natriuretic Peptide ◽  
Mrna Expression ◽  
Pulmonary Oedema ◽  
Natriuretic Peptide ◽  
Cell Function ◽  
Control Group ◽  
Sham Operation ◽  
Endothelial Cell Function ◽  
And Control ◽  
Atrial Natriuretic

Atrial natriuretic peptide (ANP) is a cardiac hormone which affects endothelial cell function through a receptor-mediated process. Pneumonectomy is a common thoracic surgical procedure that can cause pulmonary oedema in the remaining lung. Few reports have investigated the aetiology of this complication. The aim of this study was to determine the changes in ANP concentration and expression of its receptors following pneumonectomy as a possible aetiology for postpneumonectomy pulmonary oedema (PPE). We compared plasma ANP concentrations, cGMP concentrations, and natriuretic peptide receptor (NPR)-A mRNA and NPR-C mRNA expression in rat lung 3 h after pneumonectomy (n = 5) or a sham operation (n = 5). The ANP concentrations in plasma and lung tissue in the pneumonectomy group were significantly higher than in the control group (749.5 versus 202.7 pgċml-1, P < 0.01; 33.1 versus 6.8 ngċg-1 wet tissue, P < 0.01 respectively). The level of ANP mRNA expression in the pneumonectomy group was significantly higher than in the control group (1.44 versus 0.41 relative ANP mRNA expression, P < 0.05). The concentration of cGMP and the level of NPR-A mRNA expression were not significantly different between the pneumonectomy and control groups. The level of NPR-C mRNA expression in the pneumonectomy group was significantly higher than in the control group (4.17 versus 2.19 relative NPR-C mRNA expression, P < 0.01). These findings suggest that changes in pulmonary ANP and NPR-C expression may contribute to the development of PPE in the remaining lung in the acute phase following pneumonectomy.

scholarly journals Article Commentary: The Alternatively Spliced Form “b” of the Epithelial Sodium Channel α subunit (α ENaC): Any Prior Evidence of its Existence?

2010 ◽  
Vol 4 ◽  
pp. CMC.S5270
Author(s):  
Marlene F. Shehata
Keyword(s):  
Sodium Channel ◽  
Mrna Expression ◽  
Epithelial Sodium Channel ◽  
Protein Band ◽  
Sodium Balance ◽  
Kidney Cortex ◽  
Western Blots ◽  
Α Subunit ◽  
Alternatively Spliced ◽  
Additional Protein

The epithelial sodium channel (ENaC) is critical in maintaining sodium balance across aldosterone-responsive epithelia. ENaC is a combined channel formed of three subunits (αβγ) with α ENaC subunit being the most critical for channel functionality. In a previous report, we have demonstrated the existence and mRNA expression levels of four alternatively spliced forms of the α ENaC subunit denoted by -a, -b, -c and -d in kidney cortex of Dahl S and R rats. Of the four alternatively spliced forms presently identified, α ENaC-b is considered the most interesting for the following reasons: Aside from being a salt-sensitive transcript, α ENaC-b mRNA expression is ~32 fold higher than α ENaC wildtype in kidney cortex of Dahl rats. Additionally, the splice site used to generate α ENaC-b is conserved across species. Finally, α ENaC-b mRNA expression is significantly higher in salt-resistant Dahl R rats versus salt-sensitive Dahl S rats. As such, this commentary aims to highlight some of the previously published research articles that described the existence of an additional protein band on α ENaC western blots that could account for α ENaC-b in other rat species.

scholarly journals Epigenetic Downregulation of Atrial Natriuretic Peptide but not Vasopressin mRNA Expression in Females with Eating Disorders is Related to Impulsivity

2008 ◽  
Vol 33 (11) ◽  
pp. 2605-2609 ◽  
Author(s):  
Helge Frieling ◽  
Stefan Bleich ◽  
Jeannette Otten ◽  
Konstanze D Römer ◽  
Johannes Kornhuber ◽  
...  
Keyword(s):  
Eating Disorders ◽  
Atrial Natriuretic Peptide ◽  
Mrna Expression ◽  
Natriuretic Peptide ◽  
Atrial Natriuretic

Cardiac and plasma atrial natriuretic peptide after 9-day hindlimb suspension in rats

1994 ◽  
Vol 76 (2) ◽  
pp. 641-649 ◽  
Author(s):  
J. Fareh ◽  
B. Bayard ◽  
J. Gabrion ◽  
G. Thibault ◽  
J. Oliver ◽  
...  
Keyword(s):  
Atrial Natriuretic Peptide ◽  
Mrna Expression ◽  
Natriuretic Peptide ◽  
Hindlimb Suspension ◽  
Dense Granules ◽  
Atrial Cardiocytes ◽  
Male Wistar Rats ◽  
The Right ◽  
Right Atria ◽  
Atrial Natriuretic

To determine atrial natriuretic peptide (ANP) adaptation to simulated weightlessness, immunoreactive plasma (ir-NH2- and ir-COOH-terminals) and atrial (ir-COOH-terminal) ANP levels, atrial mRNA expression, immunoreactive cardiocyte ANP levels (ir-NH2- and ir-COOH-terminals), and ultrastructural observations of granules in atrial cardiocytes were assessed in male Wistar rats after a 9-day hindlimb suspension. Plasma ir-NH2- and ir-COOH-terminal ANP concentrations decreased by 17 (P < 0.05) and 37% (P < 0.05), respectively, in suspended rats. A concomitant ir-COOH-terminal ANP content reduction was also observed in left (31%; P < 0.01) and right atria (25%; P < 0.05). Atrial ANP mRNA expression was severely depleted in the right atrium and less so in the left atrium after 9 days of hindlimb suspension. Immunocytochemistry observations demonstrated lowered NH2- and COOH-terminal ANP immunoreactivities in left and right atria from suspended rats. A reduced number of storage granules (dense granules) in both atria was also noted on ultrastructural analysis. It was concluded that ANP biosynthesis, storage, and release were decreased after a 9-day hindlimb suspension.

scholarly journals Increased atrial natriuretic peptide mRNA expression in the kidney of diabetic rats

1997 ◽  
Vol 51 (4) ◽  
pp. 1100-1105 ◽  
Author(s):  
Shyi-Jang Shin ◽  
Yau-Jiunn Lee ◽  
Mian-Shin Tan ◽  
Tusty-Jiuan Hsieh ◽  
Juei-Hsiung Tsai
Keyword(s):  
Atrial Natriuretic Peptide ◽  
Mrna Expression ◽  
Natriuretic Peptide ◽  
Diabetic Rats ◽  
Atrial Natriuretic
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