scholarly journals Increased atrial natriuretic peptide mRNA expression in the kidney of diabetic rats

1997 ◽  
Vol 51 (4) ◽  
pp. 1100-1105 ◽  
Author(s):  
Shyi-Jang Shin ◽  
Yau-Jiunn Lee ◽  
Mian-Shin Tan ◽  
Tusty-Jiuan Hsieh ◽  
Juei-Hsiung Tsai
Keyword(s):  
Atrial Natriuretic Peptide ◽  
Mrna Expression ◽  
Natriuretic Peptide ◽  
Diabetic Rats ◽  
Atrial Natriuretic

scholarly journals Atrial natriuretic peptide modulates cystic fibrosis transmembrane conductance regulator chloride channel expression in rat proximal colon and human intestinal epithelial cells

2006 ◽  
Vol 189 (1) ◽  
pp. 155-165 ◽  
Author(s):  
H J Novaira ◽  
D S Ornellas ◽  
T M Ortiga-Carvalho ◽  
X M Zhang ◽  
J Souza-Menezes ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Epithelial Cells ◽  
Atrial Natriuretic Peptide ◽  
Mrna Expression ◽  
Natriuretic Peptide ◽  
Proximal Colon ◽  
Transmembrane Conductance Regulator ◽  
Transmembrane Conductance ◽  
Cystic Fibrosis Transmembrane ◽  
Atrial Natriuretic

The cystic fibrosis transmembrane conductance regulator (CFTR) is one of the most intensively investigated Cl− channels. Different mutations in the CFTR gene cause the disease cystic fibrosis (CF). CFTR is expressed in the apical membrane of various epithelial cells including the intestine. The major organ affected in CF patients is the lung, but it also causes an important dysfunction of intestinal ion transport. The modulation of CFTR mRNA expression by atrial natriuretic peptide (ANP) was investigated in rat proximal colon and in human intestinal CaCo-2 cells by RNase protection assay and semi-quantitative reverse transcriptase PCR techniques. Groups of rats subjected to volume expansion or intravenous infusion of synthetic ANP showed respective increases of 60 and 50% of CFTR mRNA expression in proximal colon. CFTR mRNA was also increased in cells treated with ANP, reaching a maximum effect at 10−9 M ANP, probably via cGMP. ANP at 10−9 M was also able to stimulate both the CFTR promoter region (by luciferase assay) and protein expression in CaCo-2 cells (by Western blot and immunoprecipitation/phosphorylation). These results suggested the involvement of ANP, a hormone involved with extracellular volume, in the expression of CFTR in rat proximal colon and CaCo-2 intestinal cells.

Changes in atrial natriuretic peptide concentration and expression of its receptors after pneumonectomy in the rat

2000 ◽  
Vol 99 (4) ◽  
pp. 343-348 ◽  
Author(s):  
Kohichi TAMURA ◽  
Shinzo TAKAMORI ◽  
Hiroharu MIFUNE ◽  
Akihiro HAYASHI ◽  
Kazuo SHIROUZU
Keyword(s):  
Atrial Natriuretic Peptide ◽  
Mrna Expression ◽  
Pulmonary Oedema ◽  
Natriuretic Peptide ◽  
Cell Function ◽  
Control Group ◽  
Sham Operation ◽  
Endothelial Cell Function ◽  
And Control ◽  
Atrial Natriuretic

Atrial natriuretic peptide (ANP) is a cardiac hormone which affects endothelial cell function through a receptor-mediated process. Pneumonectomy is a common thoracic surgical procedure that can cause pulmonary oedema in the remaining lung. Few reports have investigated the aetiology of this complication. The aim of this study was to determine the changes in ANP concentration and expression of its receptors following pneumonectomy as a possible aetiology for postpneumonectomy pulmonary oedema (PPE). We compared plasma ANP concentrations, cGMP concentrations, and natriuretic peptide receptor (NPR)-A mRNA and NPR-C mRNA expression in rat lung 3 h after pneumonectomy (n = 5) or a sham operation (n = 5). The ANP concentrations in plasma and lung tissue in the pneumonectomy group were significantly higher than in the control group (749.5 versus 202.7 pgċml-1, P < 0.01; 33.1 versus 6.8 ngċg-1 wet tissue, P < 0.01 respectively). The level of ANP mRNA expression in the pneumonectomy group was significantly higher than in the control group (1.44 versus 0.41 relative ANP mRNA expression, P < 0.05). The concentration of cGMP and the level of NPR-A mRNA expression were not significantly different between the pneumonectomy and control groups. The level of NPR-C mRNA expression in the pneumonectomy group was significantly higher than in the control group (4.17 versus 2.19 relative NPR-C mRNA expression, P < 0.01). These findings suggest that changes in pulmonary ANP and NPR-C expression may contribute to the development of PPE in the remaining lung in the acute phase following pneumonectomy.

Atrial Natriuretic Peptide and Prostacyclin Synergistically Mediate Hyperfiltration and Hyperperfusion of Diabetic Rats

Diabetes ◽  
1992 ◽  
Vol 41 (4) ◽  
pp. 533-538 ◽  
Author(s):  
N. Perico ◽  
A. Benigni ◽  
M. Gabanelli ◽  
A. Piccinelli ◽  
M. Rog ◽  
...  
Keyword(s):  
Atrial Natriuretic Peptide ◽  
Natriuretic Peptide ◽  
Diabetic Rats ◽  
Atrial Natriuretic

scholarly journals Effects of an atrial natriuretic peptide receptor antagonist on glomerular hyperfiltration in diabetic rats.

1994 ◽  
Vol 4 (8) ◽  
pp. 1564-1570 ◽  
Author(s):  
P L Zhang ◽  
H S Mackenzie ◽  
J L Troy ◽  
B M Brenner
Keyword(s):  
Atrial Natriuretic Peptide ◽  
Receptor Antagonist ◽  
Natriuretic Peptide ◽  
Experimental Diabetes ◽  
Diabetic Rats ◽  
Glomerular Hyperfiltration ◽  
Acute Effects ◽  
Experimental Diabetes Mellitus ◽  
Excretion Rates ◽  
Atrial Natriuretic

Previous studies from this laboratory implicated atrial natriuretic peptide (ANP) as a possible mediator of glomerular hyperfiltration in diabetic rats. In this study, the potential of HS-142-1 (HS), a novel polysaccharide compound with ANP receptor antagonist properties, to ameliorate glomerular hyperfiltration in diabetic rats was assessed. Initially, it was confirmed that a bolus iv injection of HS blocked both diuretic and natriuretic responses to exogenous ANP in normal Munich-Wistar rats. The acute effects of HS in moderately hyperglycemic diabetic rats with glomerular hyperfiltration and hyperperfusion were then determined. In diabetic rats, HS (20 mg/kg bolus iv) lowered GFR by approximately 46% from hyperfiltering (1.86 +/- 0.06 mL/min) to normal (1.13 +/- 0.13 mL/min) levels, whereas GFR in vehicle-treated diabetic rats remained unchanged (1.92 +/- 0.08 mL/min to 1.77 +/- 0.09 mL/min). In nondiabetic euvolemic rats, GFR was reduced by 20% after HS, whereas GFR in vehicle-treated controls was unchanged. In contrast, HS had no effect on GFR in hydropenic rats. Effective RPF was not significantly reduced in either diabetic or normal euvolemic rats in response to HS; consequently, significant reductions in filtration fraction were observed in both groups. Urine flow and sodium excretion rates were significantly reduced after HS in both diabetic and nondiabetic groups but not in hydropenic or vehicle-treated groups. These data show that HS ameliorates glomerular hyperfiltration in diabetic rats, further supporting the hypothesis that intrarenal actions of natriuretic peptides contribute significantly to glomerular hyperfiltration in experimental diabetes mellitus.

Atrial natriuretic peptide and prostacyclin synergistically mediate hyperfiltration and hyperperfusion of diabetic rats

Diabetes ◽  
1992 ◽  
Vol 41 (4) ◽  
pp. 533-538 ◽  
Author(s):  
N. Perico ◽  
A. Benigni ◽  
M. Gabanelli ◽  
A. Piccinelli ◽  
M. Rog ◽  
...  
Keyword(s):  
Atrial Natriuretic Peptide ◽  
Natriuretic Peptide ◽  
Diabetic Rats ◽  
Atrial Natriuretic

scholarly journals Epigenetic Downregulation of Atrial Natriuretic Peptide but not Vasopressin mRNA Expression in Females with Eating Disorders is Related to Impulsivity

2008 ◽  
Vol 33 (11) ◽  
pp. 2605-2609 ◽  
Author(s):  
Helge Frieling ◽  
Stefan Bleich ◽  
Jeannette Otten ◽  
Konstanze D Römer ◽  
Johannes Kornhuber ◽  
...  
Keyword(s):  
Eating Disorders ◽  
Atrial Natriuretic Peptide ◽  
Mrna Expression ◽  
Natriuretic Peptide ◽  
Atrial Natriuretic

scholarly journals The Cellular Localization of Increased Atrial Natriuretic Peptide mRNA and Immunoreactivity in Diabetic Rat Kidneys

2002 ◽  
Vol 50 (11) ◽  
pp. 1501-1507 ◽  
Author(s):  
Feng J. Lal ◽  
Ming C. Hsieh ◽  
Shih C. Hsin ◽  
Shiu R. Lin ◽  
Jinn Y. Guh ◽  
...  
Keyword(s):  
Atrial Natriuretic Peptide ◽  
Natriuretic Peptide ◽  
Cellular Localization ◽  
Diabetic Rats ◽  
Diabetic Rat ◽  
Thick Ascending Limb ◽  
Collecting Ducts ◽  
Straight Tubules ◽  
Collecting Tubules ◽  
Atrial Natriuretic

Increased intrarenal atrial natriuretic peptide (ANP) mRNA expression has been reported in several disorders. To further investigate the action of renal ANP, we need to elucidate the exact site of its alteration in diseased kidneys. ANP mRNA and ANP were detected by in situ hybridization and immunohistochemistry in the kidneys from five normal and five diabetic rats. Renal ANP mRNA in eight normal and nine diabetic rats was measured by RT-PCR with Southern blot hybridization. In normal and diabetic rats, the distribution of ANP mRNA and ANP-like peptide was mainly located in proximal, distal, and collecting tubules. However, diabetic rats had significant enhancement of ANP mRNA and ANP-immunoreactive staining in the proximal straight tubules, medullary thick ascending limbs, and medullary collecting ducts. ANP mRNA in the outer and inner medulla of nine diabetic rats increased 5.5-fold and 3.5-fold, but only 1.8-fold in the renal cortex. This preliminary study showed that ANP mRNA and ANP immunoreactivity in proximal straight tubules, medullary thick ascending limb, and medullary collecting ducts apparently increased in diabetic kidneys. These findings imply that ANP synthesis in these nephrons may involve in adaptations of renal function in diabetes.

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