scholarly journals The Effect of Mesenchymal Stem Cell Wharton's Jelly on Matrix Metalloproteinase-1 and Interleukin-4 Levels in Osteoarthritis Rat Model

2019 ◽  
Vol 7 (4) ◽  
pp. 529-535 ◽  
Author(s):  
Endrinaldi Endrinaldi ◽  
Eryati Darwin ◽  
Nasrul Zubir ◽  
Gusti Revilla
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Matrix Metalloproteinase ◽  
Joint Disease ◽  
Interleukin 4 ◽  
Target Component ◽  
Control Group ◽  
Control Group Design ◽  
Matrix Metalloproteinase 1

BACKGROUND: Osteoarthritis (OA) is generally considered a degenerative joint disease caused by biomechanical changes and the ageing process. In OA pathogenesis, the development of OA is thought to be regulated largely by excess matrix metalloproteinase (MMP), which contributes to the degradation of extracellular matrices such as MMP-1 and Interleukin-4. AIM: This study aims to prove the influence of Mesenchymal Stem Cell Wharton Jelly on decreasing MMP-1 levels and increasing IL-4 which is a specific target as a target component in cases of osteoarthritis in vivo. MATERIAL AND METHODS: This research is an experimental study with the design of Post-Test-Only Control Group Design. The sample consisted of 16 OA rats as a control group and 16 OA rats treated with MSC-WJ as a treatment group. OA induction is done by injection of monosodium iodoacetate (MIA) into the intra-articular right knee. Giving MSC-WJ is done in the third week after MIA induction. The serum MMP-1 and IL-4 levels were measured after 3 weeks treated with MSC-WJ using the ELISA method. The statistical test used is an independent t-test. The value of p < 0.05 was said to be statistically significant. RESULTS: The result showed that serum MMP-1 levels were higher in the group treated with MSC-WJ than in the control group (p < 0.05). Serum IL-4 levels were higher in the group treated with MSC-WJ than in the control group (p < 0.05). CONCLUSION: This study concluded that MSC-WJ increased MMP-1 levels and IL-4 levels in serum OA rats. MSC-WJ showed a negative effect on MMP-1 in the serum of OA rats.

scholarly journals The The Effect of Mesenchymal Stem Cell Wharton's Jelly on ADAMTS-4 and iNOS Levels in Osteoarthritis Rat Model

2019 ◽  
Vol 7 (8) ◽  
pp. 1270-1275
Author(s):  
Endrinaldi Endrinaldi ◽  
Eryati Darwin ◽  
Nasrul Zubir ◽  
Gusti Revilla
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
The Elderly ◽  
Control Group ◽  
Control Group Design ◽  
Joint Tissue ◽  
Degrading Enzymes ◽  
Post Test ◽  
Monosodium Iodoacetate

BACKGROUND: Osteoarthritis (OA) is one of the most common diseases among the elderly. OA occurs due to an imbalance between degradation and synthesis in articular joint tissue, causing changes in joint components such as cells, matrices and molecular production. Therefore, knowledge of cartilage-degrading enzymes such as ADAMTS-4 and iNOS is needed. AIM: This study aims to prove the effect of Mesenchymal Stem Cell Wharton Jelly on decreasing ADAMTS-4 levels as cartilage-degrading enzymes and increasing levels of iNOS which showed the immunosuppressive potential of MSC-WJ in cases of osteoarthritis in vivo. MATERIAL AND METHODS: This research is an experimental study with the design of Post-test-Only Control Group Design. The sample consisted of 16 OA rats as a control group and 16 OA rats treated with MSC-WJ as a treatment group. OA induction is done by injection of monosodium iodoacetate (MIA) into the intra-articular right knee. Giving MSC-WJ is done in the third week after MIA induction. The serum ADAMTS-4 and iNOS levels were measured after 3 weeks treated with MSC-WJ using the ELISA method. The statistical test used is an independent t-test. The value of p < 0.05 was said to be statistically significant. RESULT: The result showed that serum ADAMTS-4 levels were lower in the group treated with MSC-WJ than in the control group, but not statistically significant (p > 0.05). Serum iNOS levels were higher in the group treated with MSC-WJ than in the control group (p < 0.05). CONCLUSION: This study concluded that MSC-WJ significantly reduced ADAMTS-4 levels and increased the serum iNOS levels of OA rats.

scholarly journals Terminalia ferdinandiana inhibited the increased of matrix metalloproteinase-1 and prevent collagen decreased in mice skin exposed to UV-B

2021 ◽  
Vol 4 (1) ◽  
pp. 11-14
Author(s):  
Shalihaty Emy ◽  
Ida Sri Iswari ◽  
Ni Wayan Winarti
Keyword(s):  
Matrix Metalloproteinase ◽  
Ultraviolet B ◽  
Control Group ◽  
Study Group ◽  
Control Group Design ◽  
Matrix Metalloproteinase 1 ◽  
The Mean ◽  
Kakadu Plum ◽  
Terminalia Ferdinandiana

Kakadu cream contained 0,1% Kakadu plum (Terminalia ferdinandiana) extract with a high content of phenolic compound, flavonol, tocopherol, luthein, chlorophyll, and ellagic acid has potential to prevent the Ultraviolet-B (UV-B) effect on skin aging acceleration. To date, there’s no in vivo experiment on the effect of kakadu plum extract on collagen nor matrix metalloproteinase-1(MMP-1). This research aims to evaluate the effectiveness of Kakadu cream administration in inhibiting the increase of MMP-1 expressions and prevent the decrease of collagen amount in mice (Mus musculus) skin exposed to UV-B.   An experimental study with a post-test only control group design was employed in 36 male mice, 6-8 weeks old, weighing 20-25 grams. The samples were divided randomly into two groups, a control group given base cream and the study group, given Kakadu cream 0,1% on their shaved backs, 1 cm2 in size as the UV-B exposure's location. The UV-B irradiation was done three times a week for 4 weeks. The base and Kakadu cream were given twice a day. Comparative analysis was carried out to compare MMP-1 expression and collagen amount in both groups.   The results show that the mean of MMP-1 expression on the study group was significantly lower compared to control group (p<0.001). The mean collagen amount was significantly higher in the study group than in the control group (p< 0,001).   From the results, can be concluded that Kakadu cream inhibited the increase MMP-1 expression and prevent the decrease of collagen amount in mice skin exposed to UV-B.

Mesenchymal Stem Cell Therapy for Acetaminophen-Related Liver Injury: A Systematic Review and Meta-Analysis of Experimental Studies in Vivo

2021 ◽  
Vol 16 ◽  
Author(s):  
Li Wang ◽  
Yiwen Zhang ◽  
Jiajun Zhong ◽  
Yuan Zhang ◽  
Shuisheng Zhou ◽  
...  
Keyword(s):  
Systematic Review ◽  
Stem Cell ◽  
Liver Injury ◽  
Mesenchymal Stem Cell ◽  
Small Sample Size ◽  
Meta Analysis ◽  
Experimental Studies ◽  
Small Sample ◽  
Control Group

Objective: The efficacy of mesenchymal stem cell (MSC) therapy in acetaminophen-induced liver injury has been investigated in animal experiments, but individual studies with a small sample size cannot be used to draw a clear conclusion. Therefore, we conducted a systematic review and meta-analysis of preclinical studies to explore the potential of using MSCs in acetaminophen-induced liver injury. Methods: Eight databases were searched for studies reporting the effects of MSCs on acetaminophen hepatoxicity. The Preferred Reporting Items for Systematic Reviews and Meta-analyses (PRISMA) guidelines were used. SYRCLE’s risk of bias tool for animal studies was applied to assess the methodological quality. A meta-analysis was performed by using RevMan 5.4 and STATA/SE 16.0 software. Results: Eleven studies involving 159 animals were included according to PRISMA statement guidelines. Significant associations were found for MSCs with the levels of alanine transaminase (ALT) (standardized mean difference (SMD) − 2.58, p < 0.0001), aspartate aminotransferase (AST) (SMD − 1.75, p = 0.001), glutathione (GSH) (SMD 3.7, p < 0.0001), superoxide dismutase (SOD) (SMD 1.86, p = 0.022), interleukin 10 (IL-10) (SMD 5.14, p = 0.0002) and tumor necrosis factor-α (TNF-α) (SMD − 4.48, p = 0.011) compared with those in the control group. The subgroup analysis showed that the tissue source of MSCs significantly affected the therapeutic efficacy (p < 0.05). Conclusion: Our meta-analysis results demonstrate that MSCs could be a potential treatment for acetaminophen-related liver injury.

scholarly journals Pemberian tempoyak per oral dapat menghambat peningkatan ekspresi matriks metaloproteinase-1 pada tikus (Rattus norvegicus) betina dewasa galur Wistar yang dipajan sinar UVB

2017 ◽  
Vol 9 (2) ◽  
Author(s):  
Joni Fiter ◽  
AAG Putra Wiraguna ◽  
Wimpie Pangkahila
Keyword(s):  
Treatment Group ◽  
Matrix Metalloproteinase ◽  
Rattus Norvegicus ◽  
Ultraviolet B ◽  
Ultraviolet Rays ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Matrix Metalloproteinase 1 ◽  
Per Oral

Abstract: Ultraviolet B (UVB) is a source of free radicals that accelerate aging process, especially in the skin by increasing the expression of MMP-1. Tempoyak is rich in nutraceuticals and probiotics that may provide a protective effect against skin exposure to ultraviolet rays. This study was aimed to prove that oral tempoyak could inhibit the increase of matrix metalloproteinase-1 (MMP-1) in UVB-induced rats (Rattus norvegicus). This was a true experimental study with the posttest only control group design. Subjects were 36 female Wistar rats (Rattus norvegicus), aged 2.5-3 months, weighing180-200 g, divided into 2 groups. The control group (P0) exposed to UVB was given oral aquadest as placebo, while the treatment group (P1) exposed to UVB was given 1 g/200 g body weight of oral tempoyak. After 15 days of treatment, all rats were anesthetized and their skin tissues were taken for examination of MMP-1 expression. The analysis showed that the average of MMP-1 expression in the control group (P0) was 25.26±11.19% meanwhile the average of MMP-1 expression in the treatment group (P1) was 8.67±2.51%. There was a significant difference between the MMP-1 expression of the two groups (P = 0.000). Conclusion: Oral tempoyak could inhibit the increase of matrix metalloproteinase-1 (MMP-1) in UVB-induced rats (Rattus norvegicus).Keywords: tempoyak, MMP-1, UVBAbstrak: Ultraviolet B (UVB) merupakan salah satu sumber radikal bebas yang dapat mempercepat proses penuaan, khususnya penuaan kulit melalui peningkatan ekspresi MMP-1. Tempoyak yang kaya akan kandungan nutraseutikal dan probiotik dapat memberikan efek perlindungan kulit terhadap pajanan sinar ultraviolet. Penelitian ini bertujuan untuk membuktikan bahwa pemberian tempoyak per oral dapat menghambat peningkatan ekspresi MMP-1 tikus yang dipajan sinar ultraviolet-B (UVB). Jenis penelitian ialah eksperimental murni dengan menggunakan post test only control group design. Subjek penelitian ialah 36 ekor tikus putih (Rattus norvegicus), betina, galur Wistar, berumur 2,5-3 bulan, dengan berat badan 180-200 gr yang dibagi menjadi 2 (dua) kelompok masing-masing berjumlah 18 ekor tikus. Kelompok kontrol (P0) diberikan pajanan UVB dan akuades per oral sebagai plasebo sedangkan kelompok perlakuan (P1) diberikan pajanan UVB dan tempoyak dengan dosis 1 gr/ 200 gr BB. Setelah 15 hari perlakuan, seluruh tikus dianestesi kemudian diambil jaringan kulitnya untuk pemeriksaan ekspresi MMP-1 dermis. Hasil analisis menunjukkan bahwa rerata ekspresi MMP-1 pada kelompok kontrol (P0) ialah 25,26±11,19% sedangkan rerata ekspresi MMP-1 pada kelompok perlakuan (P1) ialah 8,67±2,51%. Terdapat perbedaan bermakna antara rerata ekspresi MMP-1 antara kedua kelompok (P = 0,000). Simpulan: Pemberian tempoyak per oral dapat menghambat peningkatan ekspresi MMP-1 tikus (Rattus norvegicus) yang dipajan sinar ultraviolet-B (UVB).Kata kunci: tempoyak, MMP-1, UVB

scholarly journals Establishment of an Ex Vivo Inflammatory Osteoarthritis Model With Human Osteochondral Explants

2021 ◽  
Vol 9 ◽  
Author(s):  
Kaihu Li ◽  
Penghui Zhang ◽  
Yong Zhu ◽  
Mauro Alini ◽  
Sibylle Grad ◽  
...  
Keyword(s):  
Femoral Head ◽  
Matrix Metalloproteinase ◽  
Ex Vivo ◽  
Degenerative Joint Disease ◽  
New Drugs ◽  
Joint Disease ◽  
Control Group ◽  
Pathophysiological Mechanism ◽  
Superficial Zone ◽  
Matrix Metalloproteinase 1

Osteoarthritis (OA) is the most common degenerative joint disease without clear pathophysiological mechanism and effective drugs for treatment. Although various animal models exist, the translation of the outcome into clinics remains difficult due to species differences. In this study, an ex vivo inflammatory OA model was induced using different concentrations of interleukin one beta (IL-1β) and tumor necrosis factor α (TNF-α) on explants from the human femoral head. In the inflammatory OA groups, the gene expression levels of cartilage catabolism (matrix metalloproteinase 1 (MMP1), matrix metalloproteinase 3 (MMP3)), and inflammation (interleukin 6 (IL-6), interleukin 8 (IL-8)) markers were significantly upregulated, while the anabolic genes (collagen 2 (COL2), aggrecan (ACAN), and proteoglycan 4 (PRG4)) were downregulated compared to the control group. The release of cytokines (IL-6, IL-8) and nitric oxide (NO) in the conditioned medium was also upregulated in inflammatory OA groups. The Safranin O/Fast Green staining showed loss of proteoglycan in the superficial zone cartilage after cytokine treatment. The results indicated that an ex vivo inflammation and degeneration model was successfully established using osteochondral explants from the human femoral head. This model can be used to elucidate the in-depth mechanism of inflammatory OA and to screen new drugs for OA treatment.

scholarly journals The Effect of Mesenchymal Stem Cell Wharton’s Jelly on Nuclear Factor Kappa Beta and Interleukin-10 Levels in Osteoarthritis Rat Model

2020 ◽  
Vol 8 (B) ◽  
pp. 350-357
Author(s):  
Vivi Sofia ◽  
Moch Saiful Bachri ◽  
Endrinaldi Endrinaldi
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Wharton’S Jelly ◽  
Joint Disease ◽  
Control Group ◽  
Nuclear Factor ◽  
Wharton's Jelly ◽  
Knee Oa ◽  
Nuclear Factor Kappa ◽  
Nuclear Factor Kappa Beta

BACKGROUND: Osteoarthritis (OA) is a degenerative joint disease in one or more joints characterized by changes in pathological structures such as cartilage, hypertrophy, and remodeling of the subchondral bone and secondary inflammation of the synovium membrane, causing changes in joint components such as cells, matrices, and molecular production. At the molecular level, an imbalance between catabolic and anabolic activities in joint cartilage results in OA. Nuclear factor kappa beta (NFκβ) is a cytokine that plays an important role in the signaling pathway of the pathogenesis of OA in causing an inflammatory reaction, whereas interleukin (IL)-10 is an anti-inflammatory cytokine that is involved in the pathogenesis of OA. AIM: This study aims to prove the influence before and after administration mesenchymal stem cells from Wharton’s jelly on the serum NFκβ and IL-10 levels in OA rat models. MATERIALS AND METHODS: This research is an experimental study with the design of post-test-only control group design. The sample consisted of 16 OA rats as a control group and 16 OA rats treated with MSC-WJ as a treatment group. OA induction is done by injection of monosodium iodoacetate (MIA) into the intra-articular right knee. Giving MSC-WJ is done in the 3rd week after MIA induction. The serum NFκβ and IL-10 levels were measured after 3 weeks treated with MSC-WJ using the ELISA method. The statistical test used is an independent t-test. p < 0.05 was said to be statistically significant. RESULTS: From the research results obtained, serum levels of knee OA of rat knee OA treated with mesenchymal stem cell Wharton jelly are lower than serum NFκβ levels of knee OA of the rat that is not treated, but the difference in levels of NFκβ is not significant (p > 0.05). The serum IL-10 level of rat OA of knee treated with mesenchymal stem cell Wharton jelly was higher than the serum IL-10 level of rat OA of the knee that was not treated, difference in levels of IL-10, is significant (p < 0.05). CONCLUSION: This study concluded that MSC-WJ significantly decreased the serum NFκβ levels of OA rats and not significantly increased the serum IL-10 levels of OA rats.

Ekstrak Ethanol Kulit Jeruk Purut (Citrus hystrix) Berpotensi sebagai Agen Penurun Kolesterol : Studi In Vivo

2020 ◽  
Vol 2 (1) ◽  
pp. 1
Author(s):  
Henas Deliara ◽  
Arum Kartikadewi ◽  
Dyah Mustika Nugraheni
Keyword(s):  
Random Sampling ◽  
Simple Random Sampling ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Post Test

Latar Belakang: Hiperkolesterolemia dapat menyebabkan penyakit cerebrovaskuler bahkan kematian. Salah satu pencegahan hiperkolesterolemia adalah dengan pemberian suplemen contohnya menggunakan kulit jeruk purut (Citrus hystrix) yang mengandung saponin, tanin dan flavonoid. Tujuan penelitian ini adalah membuktikan adanya pengaruh pemberian ekstrak ethanol kulit jeruk purut terhadap kadar kolesterol total pada tikus wistar jantan yang diberi diet tinggi lemak. Metode: Penelitian eksperimental  ini menggunakan metode post test only control group design. Jumlah sampel 30 ekor tikus wistar jantan dikelompokkan secara simple random sampling menjadi kelompok kontrol negative (K-), kontrol positif (K+), perlakuan 1 (P1), perlakuan 2 (P2) dan perlakuan 3 (P3). Pada kelompok K+, P1,P2 dan P3 tikus diberi minyak babi sebanyak 3 mg/200grBB/hari selama 3 minggu. Kelompok P1, P2 dan P3 diberikan ekstrak ethanol kulit jeruk purut sebesar 35 mg/200grBB/hari, 70 mg/200grBB/hari, dan 140 mg/200grBB/hari selama 3 minggu. Kadar kolesterol total dihitung dengan metode CHOD-PAP lalu dianalisis secara statistic dengan uji One Way Annova dan uji beda dengan Pos Hoc. Hasil: Dari 25 sampel, rerata kadar kolesterol total (K-):73,90 mg/dl±19,11 mg/dl; (K+):80,00 mg/dl±4,72 mg/dl; (P1):69,94 mg/dl±6,52 mg/dl; (P2):59,10 mg/dl±11,70 mg/dl; (P3):59,74 mg/dl±7,52 mg/dl. (p=0,032). Hasil uji Pos Hoc kelompok yang berbeda signifikan adalah K- dengan P2 (p=0,049), K+ dengan P2 (p=0,008) dan K+ dengan P3 (p=0,010). Kesimpulan: Terdapat pengaruh pemberian ekstrak ethanol kulit jeruk purut terhadap kadar kolesterol total pada tikus wistar jantan yang diberi diet tinggi lemak. Dosis terendah ekstrak kulit jeruk purut yang dapat menurunkan kadar kolesterol total adalah 70 mg/200 grBB/hari.

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