scholarly journals The Study of Cellular and Molecular Physiological Characteristics of Sperm in Men Living in the Aral Sea Region

2015 ◽  
Vol 4 (1) ◽  
pp. 5-8
Author(s):  
Berikbay Z. Kultanov ◽  
Raushan S. Dosmagambetova ◽  
Svetlana A. Ivasenko ◽  
Yelena S. Tatina ◽  
Assel A. Kelmyalene ◽  
...  
Keyword(s):  
Age Groups ◽  
Reproductive Function ◽  
Aral Sea ◽  
Extracellular Dna ◽  
Environmental Crisis ◽  
Chemical Pollution ◽  
Histone H2a ◽  
Dna And Rna ◽  
High Doses ◽  
Glass Stopper

BACKGROUND: Extreme environmental situation in the Aral crisis has caused a massive chemical pollution of the territory for decades with high doses of pesticides, herbicides. Discharge of industrial waste into the rivers that feed the Aral Sea has lead to the development of various pathological processes in the human body, as well as disruption of reproductive function in young men. AIM: To evaluate the performance of molecular cellular changes in the sperm of men under the influence of dust and salt aerosols in Aral Sea region.MATERIAL AND METHODS: Clinical and laboratory studies were conducted in men 5 settlements (Aralsk-city, v. Aiteke-Bi, v. Zhalagash, v. Zhusaly, v. Shieli). We have studied male ejaculate obtained after 4-5 days of abstinence, and placed it in a warm tube with a glass stopper. On the investigation proceeded ejaculate within 20-30 minutes after its preparation, during which time he was subjected to liquefaction. Isolation and quantification of ASF, RNA, DNA, and determining the fraction of histones in sperm was performed by the method of Markusheva and Savina.RESULTS: It was found that the value of ASF in the semen of men living in the zone of ecological disaster higher compared with the values of parameters in men living in the area of environmental crisis, and this trend is observed in all age groups. The study of circulating extracellular DNA and RNA in the sperm of men registered their decline with a corresponding increase of acid precursors that can be attributed to the degradation of nucleic acids under the influence of negative factors in the complex area of ecological trouble. Also, according to a study in men residing in the areas of environmental catastrophe at the age of 18-29 years, found an increased content of the H1 histone H2A lower total fraction, H3, H4 - and a sharp increase in histone H2B content - histones.CONCLUSIONS: Men living in environmentally disadvantaged areas of Kyzylorda region under the influence of dust and salt aerosols and other toxicants leads to disruption of the reproductive function in men.

scholarly journals The The Content of Extracellular Nucleic Acids in the Blood and Ejaculate of Men of Reproductive Age Living in the Ecologically Unfavourable Regions of the Aral Sea

2019 ◽  
Vol 7 (8) ◽  
pp. 1388-1390
Author(s):  
Svetlana Jangildinova ◽  
Yelena Tatina ◽  
Gulzhan Kaliyeva ◽  
Kuttykyz Kuvatbaeva ◽  
Nursaya Beygam
Keyword(s):  
Nucleic Acids ◽  
Biological Fluids ◽  
Age Groups ◽  
Group Living ◽  
Reproductive Age ◽  
Aral Sea ◽  
Extracellular Dna ◽  
Age Group ◽  
Extracellular Rna ◽  
Younger Age

AIM: We aimed to study the influence of adverse environmental factors on molecular-cellular processes in the population living in the Aral Sea region. METHODS: Extracellular RNA (ecRNA) and ecDNA were determined in blood. We obtained the ejaculate of the studied men after 4-5 days of abstinence. The ejaculate was placed in a warm tube with a ground stopper. The examination of the ejaculate was started in 20-30 minutes after receiving, as during this time it is subjected to liquefaction. Spectrophotometry of ASF, RNA and DNA hydrolysates was performed on an SF 26 at a wavelength of 290 nm against H2O. RESULTS: In the ejaculate of the studied groups of men, significant deviations in the content of extracellular nucleic acid fractions from the indicators of the comparison group were also detected. Statistically significant differences in the content of extracellular RNA were observed in men of the younger age group living in the territory of all study regions. A significant increase in the content of extracellular DNA was detected in two regions, but not in all age groups. CONCLUSION: The study revealed a significant increase in the content of extracellular nucleic acids in the biological fluids of men of reproductive age living in the Aral Sea region. The most significant are the changes in the level of extracellular RNA in the blood plasma and ejaculate in men of the younger age group and the increase in ASF content in the ejaculate in men of all age groups.

scholarly journals The Impact of Dust and Salt Aerosols of the Aral Sea on the Tissues of Rat Testes in the Experiment

2020 ◽  
Vol 8 (A) ◽  
pp. 452-456
Author(s):  
Assylgul Suleimen ◽  
Roza Yesimova ◽  
Bibigul Rakhimova ◽  
Gulnaz Suleimen ◽  
Aidana Yerubay ◽  
...  
Keyword(s):  
Climate Change ◽  
Reproductive Function ◽  
Aral Sea ◽  
Male Rats ◽  
Chemical Pollution ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Negative Effect ◽  
Histological Characteristics ◽  
The Impact

BACKGROUND: The Aral Sea crisis is recognized as one of the global environmental problems of our time. The extreme environmental situation in the region is caused by massive chemical pollution of the territory for several decades by high doses of pesticides, herbicides, dumping of industrial waste into the rivers that feed the Aral Sea. As a result of the Aral Sea desiccation, aridization of the territory, climate change, and salinization of soil and water occurred. This led to increased mineralization of precipitation, climate change, the spread and deposition of dust on an area of about 25 million hectares. One of the factors in reducing fertile activity in humans is a decrease in spermatogenesis activity. Based on identified trends, WHO is forced to review the main indicators of spermograms in the direction of their reduction. AIM: To study the effect of dust and salt aerosols of the Aral Sea on reproductive function, rat testes were studied after 7 and 24 days of inhalation administration. MATERIAL AND METHODS: Some morphometric parameters of the testes were evaluated: The diameter of the convoluted seminiferous tubules and the thickness of the germinal epithelium. The obtained comparative morphological and histological characteristics of the testes of the control and experimental groups indicate the negative effect of the components of dust and salt aerosols of the Aral Sea on the reproductive function of male rats. RESULTS: The thickness of the epithelial spermatogenic layer in the second group of rats is on average 64.52 μm, which is significantly less compared to the control group (81.3 μm). The thickness of the epithelial spermatogenous layer in the third group is on average within 73.36 μm, which is significantly less than in the control group (81.3 μm), but more than in the group exposed to dust and salt aerosols for 7 days (64, 52 μm). CONCLUSION: The study revealed that the impact of dust and salt aerosols of the Aral Sea leads to a change in the morphological and histological characteristics of the testes of animals. In experimental groups, a decrease in the diameter of the convoluted seminiferous tubule and a decrease in the thickness of the epithelial spermatogenic layer were observed. This indicates the negative effect of the components of dust and salt aerosols of the Aral Sea on the reproductive function of male rats exposed to dust for 7 and 24 days.

scholarly journals CLINICAL LABORATORY INVESTIGATION OF REPRODUCTIVE HEALTH OF WOMEN LIVING IN ECOLOGICALLY UNFAVORABLE REGIONS OF KAZAKHSTAN

2018 ◽  
Vol 97 (4) ◽  
pp. 297-300
Author(s):  
Ya. G. Turdybekova ◽  
A. A. Kelmyalene ◽  
Berikbai Zh. Kultanov
Keyword(s):  
Reproductive Health ◽  
Clinical Laboratory ◽  
Inflammatory Diseases ◽  
Ecological Factors ◽  
Aral Sea ◽  
Environmental Crisis ◽  
Anthropogenic Factors ◽  
Female Population ◽  
The Impact ◽  
Karaganda Region

In the area under study, mainly saline and solonetsous territories. The situation negatively affects the offspring of the population living in the region Continuing the work on assessing the impact of climatic and anthropogenic factors of the Aral region on the reproductive health of the female population, we compared the reproductive health of women living in five settlements of the Kyzylorda region and two settlements in the Karaganda region. There were observed 686 women in the zone of environmental crisis, 720 women living in the zone of environmental disaster and 388 women living in the Karaganda region in areas bordering the Aral Sea region. The examination was carried out on the basis of an integrated clinical-functional and laboratory examination, taking into account regional and environmental ecological factors. In the Kyzylorda region, there is a delayed onset of menarche occurred in girls older than 16 years (39%), there is a tendency to rejuvenate the age of the onset of menopause. In the Karaganda region, late menarche occurs only in 12% of cases. A third of the examined women suffered from inflammatory diseases of female genital organs in both regions. Perinatal losses, cases of spontaneous interruption and/or undeveloped pregnancies in the anamnesis are repeated many times, in zones of the ecological catastrophe and the environmental crisis there are frequent as one in four women. However, in the Karaganda region, this value figure was almost 3 times less (14%).

scholarly journals OMPREHENSIVE ECOLOGICAL-HYGIENIC EVALUATION OF ENVIRONMENTAL OBJECTS OF THE TERRITORY UNDER HARMFUL CLIMATIC AND ANTHROPOGENIC EXPOSURE

2018 ◽  
Vol 97 (4) ◽  
pp. 293-296
Author(s):  
Gulnara R. Khanturina ◽  
Z. I. Namazbaeva ◽  
G. S. Zhumabekova ◽  
G. Zh. Seitkasymova ◽  
I. A. Fedorova
Keyword(s):  
Suspended Solids ◽  
Comprehensive Assessment ◽  
Aral Sea ◽  
Anthropogenic Factors ◽  
Chemical Pollution ◽  
Assessment Score ◽  
Critical Situation ◽  
Environmental Objects ◽  
Inorganic Chemical ◽  
Syr Darya

Adverse climatic and anthropogenic factors can affect the health of people living near the dried-up Aral Sea. There was modified the comprehensive assessment of inorganic chemical pollution of the environment of the village in the Zhosaly Kyzylorda region of Kazakhstan. The comprehensive assessment score included 5 categories: satisfactory, intense, critical, crisis, catastrophic. According to own research over 2014-2015 based on the mentioned assessment score, the comprehensive assessment score amounted to 41 points and showed a critical situation. In the air, there was observed the phenol content of 1.02 MAC, suspended solids - 0.5 MAC. The main part of the fine suspended solids sized of 1.5-2.5 mm, round shape, was formed in the process of condensation and evaporation of the sea and the Syr Darya River. In the dust, there was found the excess in silicon content of 2.8 MAC, iron - 1.3 MAC, zinc 1.1 MAC. As soil pollutants, there were detected sulfates - of 173.5 MAC, chlorides - 9.0 MAC, mercury - 0.56 MAC, nickel - 0.49 MAC. In drinking water, there was revealed cadmium excess of 1.3 MAC, nickel - 1.2 MAC, chlorides - 1.25 MPC, sulfates - 1.0 MAC. In the water of the Syr Darya River, there was revealed an excess in nickel - of 2.2 MAC, sulfates - 1.8 MAC, dissolved oxygen - 2.0 MAC. In the sediments of the Syr Darya river, the sulfate content accounted for 3.36 MAC and chlorides - 1.53 MAC. In the snow cover there was revealed an excess of manganese - 5.14MPC, chromium - 2.04 MAC, zinc - 1.2 MAC. In a critical situation a significant portion (30-50%) of the population may be in a state of exertion and adaptation surge, and the most susceptible part seems to be in a state of adaptation failure, characterized by an increase in incidence rate.

IX. Nucleinsäuregehalt und Nucleinsäuresynthese nach Röntgenbestrahlung

1966 ◽  
Vol 21 (10) ◽  
pp. 960-966 ◽  
Author(s):  
Yasuhiko Takamori ◽  
Ernst-Randolf Lochmann ◽  
Wolfgang Laskowski
Keyword(s):  
Ribosomal Rna ◽  
Rna Synthesis ◽  
Small Difference ◽  
Dry Weight ◽  
Nucleic Acid Content ◽  
X Rays ◽  
X Ray ◽  
Dna And Rna ◽  
High Doses ◽  
Different Doses

The amount of DNA and RNA per dry weight as well as the rate of RNA synthesis was determined in a series of almost isogenic and homozygous Saccharomyces strains of different ploidy which had irradiated with different doses of X-rays.It was found that the RNA content per dry weight showed only a small decrease after irradiation even with high doses. The decrease in the DNA content after irradiation is larger, and it is already maximal at the smallest X-ray dose tested (75 krad) . No further decrease could be observed even after application of 225 krad.The RNA synthesis is much more radioresistant in all strains tested (haploid-hexaploid) than the colony forming ability. X-ray doses which reduce the colony forming ability of the cells to less than 1% lead to a reduction of the RNA synthesis of only about 50 per cent. The inactivation of RNA synthesis increases with increasing irradiation doses and increasing incubation time after irradiation.There was only a small difference in the radiosensitivity of the synthesis of soluble or ribosomal RNA.Genetic effects on the radiation inactivation of the colony forming ability, previously described as “aα-effect” and “AS-effect”, show no influence on the radiosensitivity of cellular nucleic acid content and synthesis.

scholarly journals Moraxella catarrhalis NucM is an entry nuclease involved in extracellular DNA and RNA degradation, cell competence and biofilm scaffolding

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Aimee Tan ◽  
Wing-Sze Li ◽  
Anthony D. Verderosa ◽  
Luke V. Blakeway ◽  
Tsitsi D. Mubaiwa ◽  
...  
Keyword(s):  
Moraxella Catarrhalis ◽  
Rna Degradation ◽  
Extracellular Dna ◽  
Dna And Rna ◽  
Cell Competence

Decline in sexual activity in ageing men: correlation with sex hormone levels and testicular changes

1979 ◽  
Vol 11 (S6) ◽  
pp. 5-18 ◽  
Author(s):  
A. Vermeulen
Keyword(s):  
Sexual Activity ◽  
Death Rate ◽  
Sexual Interest ◽  
Age Groups ◽  
Reproductive Function ◽  
Testicular Function ◽  
Age Group ◽  
Male Phenotype ◽  
Ageing Male ◽  
Striking Fact

The menopause clearly marks the end of the reproductive phase in the female, but no comparable event of sudden discontinuity in fertility occurs in the male. Successful paternity in man has been recorded at the age of 94 (Seymour, Duffy & Koerner, 1935). This difference between the sexes is rather surprising, as the male does not seem to age in a fundamentally different way from the female and as, moreover, the death rate for males is higher in all age groups, than for females. This may be related to the presence of only one X chromosome, the eventual defects of which cannot be compensated for by the activity of the homologue. The Y chromosome does not appear to bear vital genes, as it is not essential for life, XO individuals being perfectly viable, in distinction to the YO genotype. Moreover in the absence of male inducers, the phenotype is female, suggesting that the male phenotype is a more complex differentiation. For whatever reason, the lower viability of the male remains a striking fact: males represent only 35% of the age group over 75 years. The persistence of reproductive function into old age is therefore the more surprising. This does not mean, however, that a gradual decrease in sexual activity and testicular function in the ageing male does not occur. From adolescence onwards, there is a continuous decline in sexual interest, arousal and activity, without a sudden discontinuity in any age group.

scholarly journals The biofilm matrix scaffold of Pseudomonas species contains non-canonically base paired extracellular DNA and RNA

2019 ◽  
Author(s):  
Thomas Seviour ◽  
Fernaldo Richtia Winnerdy ◽  
Lan Li Wong ◽  
Xiangyan Shi ◽  
Sudarsan Mugunthan ◽  
...  
Keyword(s):  
Viscoelastic Properties ◽  
Extracellular Dna ◽  
Chromosomal Dna ◽  
Base Pairs ◽  
Matrix Component ◽  
Dna And Rna ◽  
Pseudomonas Species ◽  
The Matrix ◽  
Nucleic Acid Conformation ◽  
Biofilm Matrix

AbstractWhile extracellular DNA (eDNA) is recognized as a critical biofilm matrix component, it is not understood how it contributes to biofilm function. Here we isolate eDNA from Pseudomonas biofilms using ionic liquids, and discover that its key biophysical signatures, i.e. fluid viscoelasticity, nucleic acid conformation, and temperature and pH dependencies of gel to solution transitions, are maintained. Solid-state analysis of isolated eDNA, as a proxy for eDNA structure in biofilms, revealed non-canonical Hoogsteen base pairs, triads or tetrads involving guanine and thymine or uracil. These were less abundant in chromosomal DNA and undetected as eDNA underwent gel-sol transition. Purine-rich RNA was present in the eDNA network, which potentially enables eDNA to be the main cross-linking exopolymer in the matrix through non-canonical nucleobase interactions. Our study suggests that Pseudomonas assemble extracellular DNA and RNA into a network with viscoelastic properties, which underpin their persistence and spreading, and may aid the development of more effective controls for biofilm-associated infections.

scholarly journals An analysis of deviations from international standards of physique development in male youngsters from the former aral sea region

2020 ◽  
Vol 11 (6) ◽  
pp. 21-28
Author(s):  
Valerii O. Yerkudov ◽  
Andrey P. Pugovkin ◽  
Azat T. Matchanov ◽  
Kenzhabek U. Rozumbetov ◽  
Ruslan K. Dauletov ◽  
...  
Keyword(s):  
Body Length ◽  
Local Population ◽  
Endocrine Disrupting Chemicals ◽  
Total Body Length ◽  
Critical Zone ◽  
International Standards ◽  
Aral Sea ◽  
Chemical Pollution ◽  
Exact Test ◽  
Ecological Disaster

Introduction. According to the commonly accepted classification, geographical division of the Southern Near-Aral region (Karakalpakstan, Republic of Uzbekistan) is determined by the distance from the epicenter of the formal Aral Sea ecological disaster. The physique development was compared in male youngsters living in Zone No. 1 (critical), Zone No. 2 (stable) and Zone No. 3 (normal). The aim was a comparison of body length and mass in male youngsters up to 17 years old living in all three zones. Material and methods. Total body length and mass were studied in 320 male volunteers, age 1719. Z-index of body length and body mass index (BMI) were estimated according to the standards of WHO Growth Reference, 2007. Batch-to-batch variations were studied with KruskalWallis test, and MannWhitney criterion including Bonferroni correction for multiple comparison. Categorical features were compared using Fishers exact test for 33 contingency tables with calculating the proportion of children with one or another deviation in body weight. The results were considered statistically significant at p 0.05. Results. Subjects from Zone 1 possessed significantly lower valued of BMI and Z-index. The incidence of Z-index values lower than mean (less 1 SD) were significantly higher in Zone 1 (critical). Conclusion. The results may be explained by via chemical pollution of the environment by the endocrine-disrupting chemicals in the critical zone of the Aral See region ecological disaster and can be implied for the health monitoring of the local population.

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