scholarly journals The The Content of Extracellular Nucleic Acids in the Blood and Ejaculate of Men of Reproductive Age Living in the Ecologically Unfavourable Regions of the Aral Sea

2019 ◽  
Vol 7 (8) ◽  
pp. 1388-1390
Author(s):  
Svetlana Jangildinova ◽  
Yelena Tatina ◽  
Gulzhan Kaliyeva ◽  
Kuttykyz Kuvatbaeva ◽  
Nursaya Beygam

AIM: We aimed to study the influence of adverse environmental factors on molecular-cellular processes in the population living in the Aral Sea region. METHODS: Extracellular RNA (ecRNA) and ecDNA were determined in blood. We obtained the ejaculate of the studied men after 4-5 days of abstinence. The ejaculate was placed in a warm tube with a ground stopper. The examination of the ejaculate was started in 20-30 minutes after receiving, as during this time it is subjected to liquefaction. Spectrophotometry of ASF, RNA and DNA hydrolysates was performed on an SF 26 at a wavelength of 290 nm against H2O. RESULTS: In the ejaculate of the studied groups of men, significant deviations in the content of extracellular nucleic acid fractions from the indicators of the comparison group were also detected. Statistically significant differences in the content of extracellular RNA were observed in men of the younger age group living in the territory of all study regions. A significant increase in the content of extracellular DNA was detected in two regions, but not in all age groups. CONCLUSION: The study revealed a significant increase in the content of extracellular nucleic acids in the biological fluids of men of reproductive age living in the Aral Sea region. The most significant are the changes in the level of extracellular RNA in the blood plasma and ejaculate in men of the younger age group and the increase in ASF content in the ejaculate in men of all age groups.

2012 ◽  
Vol 141 (10) ◽  
pp. 2192-2195 ◽  
Author(s):  
M. MOSTI ◽  
B. PINTO ◽  
A. GIROMELLA ◽  
S. FABIANI ◽  
R. CRISTOFANI ◽  
...  

SUMMARYThis study aimed to estimate possible changes in seroprevalence of anti-Toxoplasma gondii IgG and IgM antibodies in people living in the area of Massa and Carrara (central Italy), in recent years. Serum samples from over 13 000 individuals were tested for both IgG and IgM anti-Toxoplasma antibodies using an immunoenzymatic method (Access® Toxo IgG, and Access® Toxo IgM II, Beckman Coulter Inc., USA). Our survey showed a decreasing trend of overall seroprevalence of 24·4% [95% confidence interval (CI) 22·62–25·71] in 2010 compared to 31·0% (95% CI 29·29–32·72) in 2007. A positive trend according to age was found, with low positivity observed in younger age groups. For women of reproductive age the prevalence of IgG antibodies was 30·2% (95% CI 28·44–31·96) in 2007 and 23·6% (95% CI 22·05–25·20) in 2010. IgM seroprevalence in women of this age group also progressively decreased from 1·6% to 0·97% during the study period. Our study confirms a decline of toxoplasmosis in Western countries.


2016 ◽  
Vol 04 (01) ◽  
pp. 023-030
Author(s):  
Vimal Kumar ◽  
Pallak Arora ◽  
Manish Khatri ◽  
Shivani Sharma ◽  
Sumit Malhotra ◽  
...  

Abstract Objective: To estimate the prevalence of periodontal disease with different indices. Methods & materials: The study population consisted of multistage stratified random sample of 1300 subjects from total population of district Ghaziabad. A cross-sectional study was conducted with multi stage stratified random sampling techniques to select the sample population. The subjects were divided into different age groups and the periodontal assessment was made on the basis of CPITN index and ESI Index. Results: The CPITN has shown to estimate incorrect periodontal disease prevalence because of its underestimation of the disease severity. A huge difference was noticed in the prevalence rate of periodontitis when subjects were examined with ESI index. Conclusion Periodontal disease was found to be highly prevalent in the study population and severity of disease increased with age. More number of subjects in younger age group were found to be healthy.


1970 ◽  
Vol 22 (1) ◽  
pp. 29-31 ◽  
Author(s):  
Md Atiqur Rahman ◽  
Sultana Rokeya Mannan

The present study was conducted to know the knowledge, attitude and practices of 300 married women in the reproductive age group living in different districts of Bangladesh, regarding HBV infection. Only 20% of the women were found aware of the mode of transmission of HBV. However, 50% of the women were having the misconceptions regarding mode of transmission of HBV. 4% of women, 30% of children up to 5 years and 15% of children above 5 years were fully immunized with hepatitis B vaccine. 80% of children up to 5 years and 75% of children above 5 years were fully immunized as per universal immunization programme. Hence, the results of the study clearly indicated the low immunization rate with vaccine against HBV under universal immunization programme and this further potentiated the need to grow public awareness about vaccination against Hepatitis B by focusing the beneficial effect of early immunization through public electronic media. Key words: KAP; HBV; Immunization; Perinatal transmission DOI: 10.3329/medtoday.v22i1.5602 Medicine Today Vol.22(1) 2010. 29-31


2022 ◽  
Vol 8 (1) ◽  
Author(s):  
Till Koopmann ◽  
Franziska Lath ◽  
Dirk Büsch ◽  
Jörg Schorer

Abstract Background Research on talent in sports aims to identify predictors of future performance. This study retrospectively investigated 1) relationships between young handball field players’ technical throwing skills and (a) their potential nomination to youth national teams and (b) their long-term career attainment 10 years later, and 2) associations between nomination status and career attainment. Results Results from retrospectively predicting nomination status and career attainment using logistic regression analyses show that technical throwing skills were partly able to explain players’ nomination status (Nagelkerke R2: females 9.2%, males 13.1%) and career attainment (Nagelkerke R2: 9.8% for female players). Here, variables throwing velocity and time on exercise showed statistically significant effects. In addition, nomination status and career attainment were shown to be associated using chi-square tests (w of .37 and .23 for female and male players, respectively) and nomination status as a predictor increased the prediction of career attainment remarkably (Nagelkerke R2: females 20.3%, males 12.7%). Conclusions Given these results, basic technical throwing skills may serve rather as a prerequisite in this age group on national level, emphasizing its importance already on lower levels and in younger age groups. Furthermore, advantages from entering the national TID system early especially for females are discussed.


2019 ◽  
Vol 1 (Number 2) ◽  
pp. 6-10
Author(s):  
Monowar Ahmad Tarafdar ◽  
Nadia Begum ◽  
Shila Rani Das ◽  
Sultana Begum ◽  
Mehruba Afrin ◽  
...  

This is a cross sectional study conducted among Currently Married Women of Reproductive Age (CMWRA) with a sample size of 476 selected purposively using a semi-structured questionnaire in 2018 at Moulvibazar Sadar to explore the factors affecting fertility. The result shows that 55.26% respondents were within 35 years age whereas only 4.28% from age group 46-49 years age group, 33% of the respondents got married at <16 years of age and 18.70% were illiterate, 47.90% had primary education. It is evident that 76% of the respondents were from rural area; 88% were Muslims, 29.41% from lower middle class followed by upper middle class (25.42%) and poorest comprised only 7.56%. The result explored that 73.91% of the respondents were from age group 41-45 got married before 16 years of age followed by 36-40 years (68.24%), 46-49 years (66%), 20-25 and 26-30 years age groups 52.38% and 52.75% respectively; 73.33% of respondents from rural area got married at <16 years of age, 68.42% of the Muslim at <16 years. The study explored that 96.39% from poorer section and 83.33% from poorest section got married at <16 years of age. Current study revealed that 82.91% of the respondents having secondary education got married before 16 years of age followed by illiterate (82.02%). It is explored that the age at marriage is statistically associated with residence, education, wealth index and religion (p= 0.001, 0.03, 0.001, 0.001 respectively). We conclude that the sociodemographic condition contributes mostly to fertility differentials in Bangladesh.


2019 ◽  
Vol 6 (1) ◽  
pp. 101
Author(s):  
Pallavi Panchu ◽  
Biju Bahuleyan ◽  
Rose Babu ◽  
Vineetha Vijayan

Background: Adipose tissue mainly visceral fat is said to be harmful and acts as a harbinger of metabolic disorders. A changing trend is seen in the recent decades with decreasing incidence of metabolic disorders in men even though visceral fat is said to be higher in them. Sex hormones may influence the deposition pattern of adipose tissue. The aim of this study was to observe effects of age on visceral fat and to know if the difference in gender pattern of fat distribution is maintained throughout life or disappears after menopause.Methods: This cross-sectional observational study was conducted in Thrissur on 385 apparently healthy subjects using Omron body composition analyser. Data was analysed using SPSS 20.0 version. The tests employed were NOVA, independent samples t-test.Results: In each age group, men had significantly higher visceral fat than females. As age increased, visceral fat increased significantly in both genders. In each group, except for younger age groups, VF levels were equal in men and women.Conclusions: Visceral fat is higher in men and this difference is seen in all age groups. As age increases, visceral fat levels also increased in men and women. The distribution of visceral fat is such that a greater number of men have high to very high levels at a younger age group, a feature observed in women only in the peri and post-menopausal age. Adoption of an active lifestyle coupled with healthy diet should protect against onset of metabolic disorders.


2015 ◽  
Vol 2015 ◽  
pp. 1-5
Author(s):  
Purva Sarkate ◽  
Supriya Paranjpe ◽  
Nayana Ingole ◽  
Preeti Mehta

Introduction. Burden of HIV in pregnant women follows overall epidemic in India. Hence, it is imperative that prevalence calculations in this group be accurate. The present study was carried out to determine prevalence of HIV in pregnant women attending our hospital, to determine trend of HIV infection and to compare our results with reported prevalence. Methods. All pregnant women are routinely counselled for HIV testing using opt-out strategy. Year-wise positivity and trend were determined in these patients over a period of five years. The positivity in different age groups was determined. Results. 31,609 women were tested of which 279 (0.88%) were positive. Positivity showed a declining trend over study period and significant quadratic trend (biphasic, P<0.05) was observed. The positivity in older age group ≥35 years (1.64%) was significantly more than younger age groups (0.76% in 15–24-year and 0.94% in 25–34-year age group) (P=0.0052). Conclusion. A significant decline in HIV positivity was seen over the study period. Taking into account heterogeneous nature of HIV epidemic even within the same district, analysis at local levels especially using the prevention of parent to child transmission of HIV program data is critical for HIV programming and resource allocation.


1992 ◽  
Vol 262 (3) ◽  
pp. H684-H690 ◽  
Author(s):  
M. L. Tod ◽  
K. Yoshimura ◽  
L. J. Rubin

Previously we reported that pulmonary vascular pressure gradients determined by vascular occlusion varied as a function of neonatal age. The purpose of this study was to evaluate the effect of blood flow on pressure gradients during normoxia (inspired O2 28%) and hypoxia (inspired O2 4.2%) in isolated, indomethacin-treated lungs obtained from lambs at less than 1, 2-4, 12-14, and 30-32 days of age (n = 6 at each age). The total pulmonary pressure gradient was partitioned into pressure gradients across relatively indistensible arteries and veins and relatively compliant vessels in the middle region (delta Pm) by inflow and outflow occlusions at flows of 25, 50, 75, and 100 ml.kg-1.min-1. During normoxia, lungs from lambs less than 1 day of age had significantly greater pressures at each flow than lungs from older lambs, due primarily to a significantly greater delta Pm in this age group. During hypoxia, the greatest vasoconstriction occurred in the youngest group, also due mostly to an increased delta Pm. This enhanced response to hypoxia in the younger age groups was due to inhibition of prostaglandin synthesis; without indomethacin treatment, the older age groups had a greater hypoxic pressor response than the younger groups. The arterial vascular resistance at all ages was relatively constant as a function of blood flow during both normoxia and hypoxia, whereas the total and middle vascular resistances generally decreased as a function of flow. The venous resistance initially decreased and then was constant. These results suggest that the neonatal pulmonary circulation h as a fixed arterial resistance, a slightly distensible venous region at low flows, and a more distensible middle region.


2015 ◽  
Vol 7 (1) ◽  
Author(s):  
Małgorzata Bronikowska ◽  
Michał Bronikowski ◽  
Agata Glapa ◽  
Bartosz Prabucki

SummaryStudy aim: the purpose of the present study was to investigate the potential of traditional games as a method of increasing the physical activity (PA) of women following mastectomy. A cross-sectional survey during the 20th ONCO Games was conducted and the sample included data from women divided into two groups: up to 50 years old (n = 26) and over 50 years old (n = 86).Material and methods: PA was assessed using a questionnaire, and the number of steps taking during 30-minute periods was measured using pedometers during each of the games. Exertion was evaluated using Borg’s Scale.Results: there was no difference in levels of coherence between the age groups. The group of younger women were statistically more active (M = 3.34 days/week) than the older group (M = 2.77 days/week). All the games were evaluated to be of light intensity, with ringo perceived as being statistically more fatiguing in the over 50 age group. The highest number of steps was performed during the game of ring-net-ball (M = 1903 steps/30 min) in both age groups, with older women performing statistically more steps. Similar situations were observed in the cases of pétanque and speedminton. In ringo, the situation was reversed. The lowest number of steps was recorded in pétanque in the younger age group (M = 296 steps/30 min).Conclusion: this study indicates that in the rehabilitation and recovery process after mastectomy, traditional games could be a bridging link between exercising and sports as they provide not just activity but also a unique and valuable social context.


Author(s):  
Nitin H Shah ◽  
Aditi Vivek Joshi ◽  
Sunita Mourya

An adnexal mass is a common entity in the reproductive age group. Ovarian masses form a majority of this condition. Tuberculosis is a disease commonly encountered in the Tropics and is endemic to India. Abdominopelvic affliction of this disease is common in women especially in the reproductive age groups. It may present with variety of non-specific clinical features and often poses an enormous diagnostic dilemma Author report one such case, presenting with a clinical impression of ovarian malignancy, however, was diagnosed to be a case of extensive pelvic tuberculosis on laparoscopy. Tissue biopsy was consistent with the finding of tuberculosis.


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