scholarly journals The in-vitro antimicrobial activity of some medicinal plants against β-lactam-resistant bacteria

2009 ◽  
Vol 3 (09) ◽  
pp. 671-680 ◽  
Author(s):  
Joseph Gangoue-Pieboji ◽  
Noelly Eze ◽  
Arnaud Ngongang Djintchui ◽  
Bathélémy Ngameni ◽  
Nolé Tsabang ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Medicinal Plants ◽  
Plant Extracts ◽  
Klebsiella Oxytoca ◽  
Antimicrobial Activities ◽  
Resistant Bacteria ◽  
Beta Lactam ◽  
Gram Negative ◽  
Garcinia Kola

Background: In effort to identify novel bacterial agents, this study was initiated to evaluate the antimicrobial properties of 17 crude extracts from 12 medicinal plants against beta-lactam-resistant bacteria. Methodology: The antimicrobial activities of plant extracts were evaluated against clinically proved beta-lactam-resistant bacteria (Klebsiella pneumoniae, Klebsiella oxytoca, Enterobacter cloacae, Serratia marcescens, Acinetobacter baumannii, Staphylococcus aureus and Enterococcus sp.) and reference strains of bacteria (Escherichia coli ATCC 35218, Enterobacter aerogenes ATCC 29751, E. aerogenes ATCC 13048, Pseudomonas aeruginosa ATCC 27853 and Enterococcus hirae ATCC 9790) by using disc-diffusion and agar-dilution assays. Results: The crude plant extracts demonstrated broad spectrum activity against all bacteria tested with inhibition zones in the range of 8-30 mm. The minimal inhibitory concentration (MIC) values of different plant extracts against the tested bacteria were found to range from ≤ 0.3 to ≥ 10 mg ml-1. The most active plant extracts were from Dortenia picta and Bridelia micrantha (MIC: 1.25-10 mg ml-1) on beta-lactam-resistant Gram-negative bacilli and the extracts from B. micrantha, Mallotus oppositifolius, Garcinia lucida, Garcinia. kola, Campylospermum densiflorum (leaves) and C. zenkeri (root) on beta-lactam-resistant Gram-positive cocci (MIC: ≤ 0.3-5 mg ml-1). Conclusion: Of the 17 plant extracts studied, seven showed good antimicrobial activity against the tested bacteria. The stem bark of B. micrantha and the leaves of D. picta were most active towards beta-lactamase producing Gram-negative bacilli. This study shows that medicinal plants could be sources of compounds which can be used to fight against beta-lactam resistant bacteria.

scholarly journals The Pomegranate: Effects on Bacteria and Viruses That Influence Human Health

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Amy B. Howell ◽  
Doris H. D'Souza
Keyword(s):  
Antimicrobial Activity ◽  
Foodborne Pathogens ◽  
Clinical Results ◽  
Multidrug Resistant ◽  
Antimicrobial Activities ◽  
Oral Bacteria ◽  
Resistant Bacteria ◽  
Wide Range

Pomegranates have been known for hundreds of years for their multiple health benefits, including antimicrobial activity. The recent surge in multidrug-resistant bacteria and the possibility of widespread global virus pandemics necessitate the need for additional preventative and therapeutic options to conventional drugs. Research indicates that pomegranates and their extracts may serve as natural alternatives due to their potency against a wide range of bacterial and viral pathogens. Nearly every part of the pomegranate plant has been tested for antimicrobial activities, including the fruit juice, peel, arils, flowers, and bark. Many studies have utilized pomegranate peel with success. There are various phytochemical compounds in pomegranate that have demonstrated antimicrobial activity, but most of the studies have found that ellagic acid and larger hydrolyzable tannins, such as punicalagin, have the highest activities. In some cases the combination of the pomegranate constituents offers the most benefit. The positive clinical results on pomegranate and suppression of oral bacteria are intriguing and worthy of further study. Much of the evidence for pomegranates’ antibacterial and antiviral activities against foodborne pathogens and other infectious disease organisms comes fromin vitrocell-based assays, necessitating further confirmation ofin vivoefficacy through human clinical trials.

scholarly journals Interaction of Folk Medicinal Plants with Levofloxacin against Escherichia Coli

2020 ◽  
Vol 14 (3) ◽  
pp. 1855-1861
Author(s):  
Isra Tayseer ◽  
Hanan Azzam ◽  
Nehaya Al-Karablieh ◽  
Amal Mayyas ◽  
Talal Aburjai
Keyword(s):  
Escherichia Coli ◽  
Medicinal Plants ◽  
Plant Extracts ◽  
Curcuma Longa ◽  
Antimicrobial Activities ◽  
Thymus Vulgaris ◽  
Additive Interaction ◽  
Traditional Use ◽  
E Coli

The present study was conducted to assess the in vitro activities of folk medicinal plants in combination with levofloxacin against TG1 and mutant KAM3-1(∆acrB-∆tolC) Escherichia coli strains. Plants were chosen based on their traditional use in combination with antibiotics among laymen. Standard protocols were followed to examine the antimicrobial activity of plant extracts and levofloxacin against E. coli in term of their minimum inhibitory concentrations (MICs) and to evaluate the plant extracts-levofloxacin interaction using checkerboard method. Among the twelve plants investigated, Thymus vulgaris, Zingiber officinale, Teucrium polium, Matricaria chamomilla and Curcuma longa had the best antimicrobial activities against E. coli strains with MIC values at 250 μg/ml. It is noteworthy to mention that other folk plants extracts reveled no effects against E coli strains. Furthermore, additive interactions were observed between levofloxacin and T. polium or T. vulgaris against E. coli wild-type TG1 strain. There was no antagonism being observed in this study. The detection of additive interaction between the extracts and levofloxacin demonstrates the prospective of these folk medicinal plants as a source of compounds to modulate antibiotic resistance.

scholarly journals Antibacterial activity in the leaves of seven bitter medicinal plants of Bangladesh

1970 ◽  
Vol 18 ◽  
pp. 128-133 ◽  
Author(s):  
MTH Molla ◽  
MS Ahsan ◽  
MT Alam ◽  
ME Haque
Keyword(s):  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Medicinal Plants ◽  
Plant Extracts ◽  
Salmonella Typhi ◽  
Antimicrobial Activities ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Ethanolic Extracts

Context: Development of resistance in human pathogens against conventional antibiotic necessitates searching indigenous medicinal plants having antibacterial property. Seven medicinal plants used actively in folklore, ayurvedic and traditional system of medicine were selected for the evaluation of their antimicrobial activity for this study.   Objectives: Evaluation of the effectiveness of some medicinal plant extracts against four Gram-positive and five Gram-negative bacteria.  Materials and Methods: The antibacterial activity of the crude ethanolic extracts obtained from the leaves of seven medicinal plants; viz., Andrographis paniculata, Catharanthus roseus, Adhatoda vasica, Vitex vegundo, Aloe vera, Flacortia ramontchi and Nyctanthes arbortristis were tested against nine bacteria at concentrations of 300-, 400- and 500 μg/ml. Standard antibiotic disc kanamycin (30μg/ml) was used for comparison. The minimum inhibitory concentration (MIC) of ethanolic extracts of the leaves of these medicinal plants were determined by testing the extracts on four Gram-positive and five Gram-negative bacteria by serial tube dilution method.   Results: All the extracts have notable antimicrobial activities against the test organisms. The ethanolic extracts of the leaves showed the highest antimicrobial activities against Bacillus megaterium and Shigella dysenteriae for An. paniculata, Ad. vasica and Al. vera; Bacillus subtilis and Salmonella typhi for C. roseus and N. arbortristis; Staphylociccus aureus and Salmonella typhi for V. vegundo; and Bacillus subtilis and Shigella sonnei for F. ramontchi respectively. The extract of the plants had MIC values ranging from 32 to 128 mg/ml. All plant extracts showed no MIC against Shigella shiga and against Sarcina lutea only C. roseus showed MIC 128 mg/ml.   Conclusion: The results revealed that the ethanolic extracts of the plants under present investigation have notable antimicrobial activities.   Keywords: medicinal plants; antimicrobial screening; MIC; bacteria. DOI: http://dx.doi.org/10.3329/jbs.v18i0.8788 JBS 2010; 18(0): 128-133

scholarly journals A Study of Ethno-Veterinary Medicinal Plants and In Vitro Antimicrobial Activities Against Bovine Mastitis Isolated Bacterial Pathogens in Algeria

2019 ◽  
Vol 76 (2) ◽  
pp. 154
Author(s):  
Radhwane SAIDI ◽  
Nora MIMOUNE ◽  
Ratiba BAAZIZI ◽  
Mohamed Hocine BENAISSA ◽  
Djamel KHELEF ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Bovine Mastitis ◽  
Multidrug Resistant ◽  
Antimicrobial Activities ◽  
Economic Losses ◽  
Resistant Bacteria ◽  
Mentha Pulegium ◽  
Antibiotic Resistant ◽  
Marrubium Vulgare

Bovine mastitis is the most serious dairy problem in terms of economic losses to the dairy industry. In Algeria, dominates as one of the most prevalent diseases in dairy cattle among the dairy farms. Mastitis treatment with antibiotics leads to the development of antibiotic resistant strains and consumer health problem. Multidrug-resistant bacteria have become a major health issue. With new generations of virulence and resistant bacteria, we need to improve our understanding and produce novel techniques to control these pathogenic strains. In our study, the activity of several extracts from seven medicinal plants, namely Mentha pulegium, Lavandula dentate, Origanium sp, Marrubium vulgare, Salvia bicolor, Blackstonia perfoliata, and Phlomis crinita, traditionally used in Algeria was investigated against 26 methicillin resistant staphylococci and multi-resistant Escherichia coli. (E.coli) isolated from animals with mastitis manifestation by the disc diffusion method.Results revealed the potential of extracts of Salvia bicolor, Marrubium vulgare and Phlomis crinita as antibacterial agents against strains isolated from bovine mastitis and support the possible use of these phytotherapic agents in the clinical management of the disease. Further studies into their toxicity and phytochemistry are advocated.

scholarly journals Antimicrobial and antibiofilm activity of the essential oil from dried leaves of Eucalyptus staigeriana

2019 ◽  
Vol 86 ◽  
Author(s):  
Marcos Saldanha Correa ◽  
Joseli Schwambach ◽  
Michele Bertoni Mann ◽  
Jeverson Frazzon ◽  
Ana Paula Guedes Frazzon
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Clinical Samples ◽  
Resistant Bacteria ◽  
Antibiofilm Activity ◽  
Gram Positive ◽  
Promising Alternative ◽  
Gram Negative

ABSTRACT: In recent years, compounds with biological properties produced by plants have received attention as an alternative to control microorganisms. Essential oils extracted from green leaves of Eucalyptus sp. have been demonstrated to have antimicrobial activities, but so far there are no reports of antimicrobial activity of essential oils extracted from dried leaves of Eucalyptus staigeriana. So, the objectives of this study were to determine the chemical composition of the essential oils obtained from dried leaves of E. staigeriana (EOdlES) and to evaluate in vitro antimicrobial and antibiofilm activities of EOdlES against gram-positive and gram-negative, resistance and multiresistant Enterococcus faecalis isolated from food and clinical samples. The characterization of EOdlES was performed by gas chromatography-mass spectrometry (GC/MS). For this study, 26 bacterial strains were used, which included 11 reference strains and 15 antibiotic resistant and multiresistant E. faecalis strains. Antimicrobial activities of EOdlES against gram-positive and gram-negative were determined using the disc diffusion method. The minimum inhibitory concentration (MIC) value was evaluated by a microbroth dilution technique. The antibiofilm effects were assessed by microtiter plate method. As a result, 21 compounds were identified, being oxygenated monoterpenes (69.58%) the major chemical family. EOdlES showed only antimicrobial activity against gram-positive strains. E. faecalis resistant and multiresistant strains show the lowest MIC (3.12 to 6.25%), when compared with reference E. faecalis strain. EOdlES has the ability to inhibit the biofilm formation, but little or none ability to inhibit the preformed biofilm. This study demonstrates that EOdlES is a promising alternative to control important foodborne and clinic gram-positive resistant bacteria.

scholarly journals Potential of Novel Antimicrobial Peptide P3 from Bovine Erythrocytes and Its Analogs To Disrupt Bacterial MembranesIn Vitroand Display Activity against Drug-Resistant Bacteria in a Mouse Model

2015 ◽  
Vol 59 (5) ◽  
pp. 2835-2841 ◽  
Author(s):  
Qinghua Zhang ◽  
Yanzhao Xu ◽  
Qing Wang ◽  
Bolin Hang ◽  
Yawei Sun ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Body Weight ◽  
Mouse Model ◽  
Antimicrobial Activities ◽  
Resistant Bacteria ◽  
Drug Resistant ◽  
Drug Resistant Bacteria ◽  
Resistant Strains ◽  
Bovine Erythrocytes

ABSTRACTWith the emergence of many antibiotic-resistant strains worldwide, antimicrobial peptides (AMPs) are being evaluated as promising alternatives to conventional antibiotics. P3, a novel hemoglobin peptide derived from bovine erythrocytes, exhibited modest antimicrobial activityin vitro. We evaluated the antimicrobial activities of P3 and an analog, JH-3, bothin vitroandin vivo. The MICs of P3 and JH-3 ranged from 3.125 μg/ml to 50 μg/ml when a wide spectrum of bacteria was tested, including multidrug-resistant strains. P3 killed bacteria within 30 min by disrupting the bacterial cytoplasmic membrane and disturbing the intracellular calcium balance. Circular dichroism (CD) spectrometry showed that P3 assumed an α-helical conformation in bacterial lipid membranes, which was indispensable for antimicrobial activity. Importantly, the 50% lethal dose (LD50) of JH-3 was 180 mg/kg of mouse body weight after intraperitoneal (i.p.) injection, and no death was observed at any dose up to 240 mg/kg body weight following subcutaneous (s.c.) injection. Furthermore, JH-3 significantly decreased the bacterial count and rescued infected mice in a model of mouse bacteremia. In conclusion, P3 and an analog exhibited potent antimicrobial activities and relatively low toxicities in a mouse model, indicating that they may be useful for treating infections caused by drug-resistant bacteria.

scholarly journals In vitro antibacterial activity of several plant extracts and essential oils against Brucella melitensis

2014 ◽  
Vol 60 (1) ◽  
pp. 29-38
Author(s):  
Mazen Safi ◽  
Ayman Al-Mariri
Keyword(s):  
Medicinal Plants ◽  
Essential Oils ◽  
Plant Extract ◽  
Plant Extracts ◽  
Brucella Melitensis ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Antibiotic Resistant ◽  
Fluid Medium

Summary Medicinal plants are considered to be new resources for the production of agents that could act as alternatives to antibiotics in the treatment of antibiotic-resistant bacteria. The aim of this study was to evaluate the efficacy of some plants native to Syria in the treatment of brucellosis. In vitro activities of some essential oils and plant extracts of some medicinal plants against 89 Brucella melitensis isolates was determined by disc diffusion method at a concentration of 5%. The microdilution assay in the fluid medium was used to determine the MICs of essential oils and plant extracts. Among the evaluated herbs, only Thymus syriacus and Cinnamomum zeylanicum essential oils and Laurus nobilis plant extract showed a high activity against B. melitensis strains. Thus, minimal inhibitory concentration (MIC50) values for T. syriacus, C. zeylanicum, and L. nobilis against B. melitensis were 6.25, 3.125 and 6.25 μl/ml, respectively. Among studied essential oils and plant extracts, T. syriacus and C. zeylanicum essential oils, and L. nobilis plant extract were the most effective ones. Moreover, T. syriacus - C. zeylanicum combination was more effective than use of each of them alone. Then, T. syriacus and C. zeylanicum essential oils and L. nobilis plant extract could act as bactericidal agents against B. melitensis.

scholarly journals Therapeutic Effect and Mechanisms of the Novel Monosulfactam 0073

2020 ◽  
Vol 64 (10) ◽  
Author(s):  
Ying Sun ◽  
Xueyuan Liao ◽  
Zhigang Huang ◽  
Yaliu Xie ◽  
Yanbin Liu ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Serial Passage ◽  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
Infection Model ◽  
The Novel ◽  
Gram Negative ◽  
Content Type

ABSTRACT This study aimed to evaluate the antimicrobial activity of the novel monosulfactam 0073 against multidrug-resistant Gram-negative bacteria in vitro and in vivo and to characterize the mechanisms underlying 0073 activity. The in vitro activities of 0073, aztreonam, and the combination with avibactam were assessed by MIC and time-kill assays. The safety of 0073 was evaluated using 3-(4,5-dimethylthizol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and acute toxicity assays. Murine thigh infection and pneumonia models were employed to define in vivo efficacy. A penicillin-binding protein (PBP) competition assay and confocal microscopy were conducted. The inhibitory action of 0073 against β-lactamases was evaluated by the half-maximal inhibitory concentration (IC50), and resistance development was evaluated via serial passage. The monosulfactam 0073 showed promising antimicrobial activity against Enterobacteriaceae, Pseudomonas aeruginosa, and Acinetobacter baumannii isolates producing metallo-β-lactamases (MBLs) and serine β-lactamases. In preliminary experiments, compound 0073 exhibited safety both in vitro and in vivo. In the murine thigh infection model and the pneumonia models in which infection was induced by P. aeruginosa and Klebsiella pneumoniae, 0073 significantly reduced the bacterial burden. Compound 0073 targeted several PBPs and exerted inhibitory effects against some serine β-lactamases. Finally, 0073 showed a reduced propensity for resistance selection compared with that of aztreonam. The novel monosulfactam 0073 exhibited increased activity against β-lactamase-producing Gram-negative organisms compared with the activity of aztreonam and showed good safety profiles both in vitro and in vivo. The underlying mechanisms may be attributed to the affinity of 0073 for several PBPs and its inhibitory activity against some serine β-lactamases. These data indicate that 0073 represents a potential treatment for infections caused by β-lactamase-producing multidrug-resistant bacteria.

REVERSION OF ANTIBIOTIC RESISTANCE WITH BETA-LACTAMASE INHIBITOR FROM MEDICINAL PLANTS

2017 ◽  
Vol 10 (10) ◽  
pp. 204
Author(s):  
Sneha Arora ◽  
Shoma Paul Nandi
Keyword(s):  
Antibiotic Resistance ◽  
Medicinal Plants ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
Beta Lactamase ◽  
Beta Lactam ◽  
Lactamase Inhibitor

  Objective: Screening of medicinal plants for the presence of beta-lactamase inhibitor identified three plants; Terminalia chebula, Terminalia bellirica, and Ocimum tenuiflorum, extracts of which inhibit beta-lactamase enzyme in vitro. The objective of this study was to evaluate and compare beta-lactamase inhibiting potential of these plant extracts.Methods: Extracts of these plants were prepared with 6 solvents of different polarity. Beta-lactamase inhibition study was performed using antibiotic-resistant bacteria in bioassay and by micro-iodometric assay. Multidrug-resistant clinical strains of Escherichia coli and laboratory strain with plasmid carrying beta-lactamase gene as positive control were used.Results: Our results from bioassay, as well as micro-iodometric assay for enzyme activity, confirmed the presence of beta-lactamase inhibitor in these plant extracts. Among the extracts made by different solvents, hexane and ethyl acetate extract of T. chebula, hexane extract of T. bellirica, and all extracts of O. tenuiflorum except dichloromethane, possessed beta-lactamase inhibitor. Multidrug-resistant clinical isolate of E. coli AIIMS-1 could be reverted by applying 50 μg/μl of extract of all the medicinal plants. The micro-iodometric result showed highest beta-lactamase inhibition with O. tenuiflorum extracts. Comparative evaluation of the O. tenuiflorum extracts with increasing concentration of inhibitor suggests that ethyl acetate extract of O. tenuiflorum contains the highest inhibition potential, which is comparable with clavulanic acid.Conclusion: The results demonstrated that the ethyl acetate extract of O. tenuiflorum contain the highest level of beta-lactamase inhibitor, which in the future can be used as an alternative to synthetic beta-lactamase inhibitors that are presently being used to control beta-lactam antibiotic resistance

scholarly journals The In Vitro Antimicrobial Effects of Lavandula angustifolia Essential Oil in Combination with Conventional Antimicrobial Agents

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Stephanie de Rapper ◽  
Alvaro Viljoen ◽  
Sandy van Vuuren
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Antimicrobial Agents ◽  
Inhibitory Concentration ◽  
Antimicrobial Effect ◽  
Antimicrobial Activities ◽  
Lavandula Angustifolia ◽  
Gram Negative ◽  
Stock Solutions

The paper focuses on the in vitro antimicrobial activity of Lavandula angustifolia Mill. (lavender) essential oil in combination with four commercial antimicrobial agents. Stock solutions of chloramphenicol, ciprofloxacin, nystatin, and fusidic acid were tested in combination with L. angustifolia essential oil. The antimicrobial activities of the combinations were investigated against the Gram-positive bacterial strain Staphylococcus aureus (ATCC 6538) and Gram-negative Pseudomonas aeruginosa (ATCC 27858) and Candida albicans (ATCC 10231) was selected to represent the yeasts. The antimicrobial effect was performed using the minimum inhibitory concentration (MIC) microdilution assay. Isobolograms were constructed for varying ratios. The most prominent interaction was noted when L. angustifolia essential oil was combined with chloramphenicol and tested against the pathogen P. aeruginosa (ΣFIC of 0.29). Lavendula angustifolia essential oil was shown in most cases to interact synergistically with conventional antimicrobials when combined in ratios where higher volumes of L. angustifolia essential oil were incorporated into the combination.

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