scholarly journals Bovine immunodeficiency virus and bovine leukemia virus and their mixed infection in Iranian Holstein cattle

2010 ◽  
Vol 4 (09) ◽  
pp. 576-579 ◽  
Author(s):  
Gholamreza Nikbakht Brujeni ◽  
Taghi Taghi Poorbazargani ◽  
Susan Nadin-Davis ◽  
Mohammad Tolooie ◽  
Neda Barjesteh
Keyword(s):  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Flow Cytometric Analysis ◽  
Bovine Immunodeficiency Virus ◽  
Holstein Cattle ◽  
T Lymphocyte Subsets ◽  
Flow Cytometric ◽  
Immunodeficiency Virus ◽  
Study Population ◽  
Virus Strains

Introduction: Bovine immunodeficiency virus (BIV) and bovine leukemia virus (BLV) have worldwide distributions, but their prevalences in Iran are unknown. We investigated the presence of infections in Iranian Holstein cattle and determined changes in hematological values for infected animals. Methodology: Nested PCR was used on blood samples from 143 animals Holstein cattle to detect proviral BIV and BLV gag sequences. Flow cytometric analysis was performed using monoclonal antibodies (mAbs) against CD4, CD8, and CD21 bovine T lymphocyte subsets. Results: Proviral BIV and BLV gag sequences were detected in 20.3% and 17% of the animals, respectively. BIV-BLV confection was also detected in 4.2% of the study population but this was not statistically significant. Flow cytometric analysis showed that both BIV-infected cows and non-infected ones had CD4/CD8 ratios of 2.45 and 1.43, respectively, and this difference was significant. BLV infected and non-infected animals had no significant differences in their CD4/CD8 ratio. In comparison to non-infected cattle, those with both BIV and BLV had a significant decrease in their CD4/CD8 ratios (1.5 % vs. 2.3; P = 0.01). Conclusion: This is the first report of BIV and BLV infections in Iran. We found no evidence that infection with one agent predisposed an animal to infection with the other. BIV infection may have a role in decreasing T CD8 counts, but this may depend on the genetics of the cattle and virus strains involved.

Vertical transmission of bovine leukemia virus and bovine immunodeficiency virus in dairy cattle herds

2002 ◽  
Vol 84 (3) ◽  
pp. 275-282 ◽  
Author(s):  
Sothy Meas ◽  
Tatsufumi Usui ◽  
Kazuhiko Ohashi ◽  
Chihiro Sugimoto ◽  
Misao Onuma
Keyword(s):  
Dairy Cattle ◽  
Vertical Transmission ◽  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Bovine Immunodeficiency Virus ◽  
Immunodeficiency Virus ◽  
Cattle Herds

scholarly journals Seroprevalence of Bovine Immunodeficiency Virus and Bovine Leukemia Virus in Draught Animals in Cambodia.

2000 ◽  
Vol 62 (7) ◽  
pp. 779-781 ◽  
Author(s):  
Sothy MEAS ◽  
Kazuhiko OHASHI ◽  
Sothyra TUM ◽  
Manov CHHIN ◽  
Kuyhor TE ◽  
...  
Keyword(s):  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Bovine Immunodeficiency Virus ◽  
Immunodeficiency Virus ◽  
Draught Animals

scholarly journals Infection of Bovine Immunodeficiency Virus and Bovine Leukemia Virus in Water Buffalo and Cattle Populations in Pakistan

2000 ◽  
Vol 62 (3) ◽  
pp. 329-331 ◽  
Author(s):  
Sothy MEAS ◽  
Junji SETO ◽  
Chihiro SUGIMOTO ◽  
Muhammad BAKHSH ◽  
Muhammad RIAZ ◽  
...  
Keyword(s):  
Bovine Leukemia Virus ◽  
Water Buffalo ◽  
Leukemia Virus ◽  
Bovine Immunodeficiency Virus ◽  
Immunodeficiency Virus

scholarly journals Seroprevalence of Bovine Immunodeficiency Virus and Bovine Leukemia Virus in Dairy and Beef Cattle in Hokkaido

2003 ◽  
Vol 65 (2) ◽  
pp. 287-289 ◽  
Author(s):  
Tatsufumi USUI ◽  
Sothy MEAS ◽  
Satoru KONNAI ◽  
Kazuhiko OHASHI ◽  
Misao ONUMA
Keyword(s):  
Beef Cattle ◽  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Bovine Immunodeficiency Virus ◽  
Immunodeficiency Virus

scholarly journals Flow cytometric analysis of T lymphocytes and cytokines in aqueous humor of patients with varicella zoster virus-mediated acute retinal necrosis

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Hao Kang ◽  
Yunbo Wei ◽  
Ming Liu ◽  
Di Yu ◽  
Yong Tao
Keyword(s):  
T Lymphocytes ◽  
Varicella Zoster Virus ◽  
Aqueous Humor ◽  
T Lymphocyte ◽  
Lymphocyte Subsets ◽  
Flow Cytometric Analysis ◽  
T Lymphocyte Subsets ◽  
Varicella Zoster ◽  
Flow Cytometric ◽  
Cd8 T Lymphocytes

Abstract Background The purpose of this study is to investigate the aqueous humor (AH) T lymphocyte subsets and cytokines of acute retinal necrosis (ARN) to elucidate the immunologic inflammatory features of this disorder. Methods Three patients with ARN infected with varicella zoster virus (VZV) who underwent multiple intravitreal injections of ganciclovir were enrolled in this study. The control group consisted of four non-infectious patients with acute anterior uveitis (AAU). Flow cytometric analysis was performed on the lymphocyte subsets from the AH and peripheral blood (PB) samples during the active phase of intraocular inflammation. Five inflammatory cytokines were measured in each AH sample and various clinical characteristics were also assessed. Results VZV deoxyribonucleic acid (DNA) was detected by real-time polymerase chain reaction (PCR) in AH from all the ARN patients, who showed higher CD8+ T lymphocytes population in AH than the AAU patients (p = 0.006). CD4/CD8 ratios of T lymphocytes and the percentage of CD8 + CD25+ T lymphocytes in AH were significantly lower in ARN than in AAU (p = 0.006; p = 0.012). In the ARN patients, the percentages of CD4+ and CD8+ T lymphocytes in AH were higher than those found in PB. The percentage of CD4 + CD25+ T lymphocytes in AH was significantly higher than the proportion in PB in the AAU patients (p = 0.001). Immunoregulatory cytokine Interleukin-10 in AH was significantly elevated in the ARN patients in comparison with the case of the AAU patients (p = 0.036). In ARN, the copy number of VZV DNA in AH positively correlated with the percentage of CD8+ T lymphocytes in AH and negatively correlated with the CD4/CD8 ratio in AH during the course of disease treatment (p = 0.009, r = 0.92; p = 0.039, r = − 0.834). Conclusion The ARN patients caused by VZV had different intraocular T lymphocyte subsets and cytokines profile than those of the non-infectious patients. High percentages of CD8+ T lymphocytes and low CD4/CD8 T cell ratios may be a potential biomarker for diagnosis of viral-infectious uveitis. T lymphocytes examination at the inflammatory sites has the potential to become a useful research tool for differentiating viral and non-viral uveitis.

Flow Cytometric Analysis of T Lymphocytes and Cytokines in Aqueous Humor of Patients with Varicella Zoster Virus-mediated Acute Retinal Necrosis

2020 ◽  
Author(s):  
Hao Kang ◽  
Yunbo Wei ◽  
Ming Liu ◽  
Di Yu ◽  
Yong Tao
Keyword(s):  
T Lymphocytes ◽  
Varicella Zoster Virus ◽  
Aqueous Humor ◽  
T Lymphocyte ◽  
Lymphocyte Subsets ◽  
Flow Cytometric Analysis ◽  
T Lymphocyte Subsets ◽  
Varicella Zoster ◽  
Flow Cytometric ◽  
Cd8 T Lymphocytes

Abstract Background: The purpose of this study is to investigate the aqueous humor (AH) T lymphocyte subsets and cytokines of acute retinal necrosis (ARN) to elucidate the immunologic inflammatory features of this disorder.Methods: Three patients with ARN infected with varicella zoster virus (VZV) who underwent multiple intravitreal injections of ganciclovir were enrolled in this study. The control group consisted of four non-viral infectious patients with acute anterior uveitis (AAU). Flow cytometric analysis was performed on the lymphocyte subsets from the AH and peripheral blood (PB) samples during the active phase of intraocular inflammation. Five inflammatory cytokines were measured in each AH sample and various clinical characteristics were also assessed.Results: VZV DNA was detected by real-time polymerase chain reaction (PCR) in AH from all the ARN patients, who showed higher CD8+ T lymphocytes population in AH than the AAU patients (p=0.006). CD4/CD8 ratios of T lymphocytes and the percentage of CD8+CD25+ T lymphocytes in AH were significantly lower in ARN than in AAU (p=0.006; p=0.012). In the ARN patients, the percentages of CD4+ and CD8+ T lymphocytes in AH were higher than those found in PB. The percentage of CD4+CD25+ T lymphocytes in AH was significantly higher than the proportion in PB in the AAU patients (p=0.001). Immunoregulatory cytokine Interleukin-10 in AH was significantly elevated in the ARN patients in comparison with the case of the AAU patients (p=0.036). In ARN, the copy number of VZV DNA in AH positively correlated with the percentage of CD8+ T lymphocytes in AH and negatively correlated with the CD4/CD8 ratio in AH during the course of disease treatment (p=0.009, r=0.92; p=0.039, r=-0.834).Conclusion: The ARN patients caused by VZV had different intraocular T lymphocyte subsets and cytokines profile than those of the non-viral infectious patients. High percentages of CD8+ T lymphocytes and low CD4/CD8 T cell ratios may be a potential biomarker for diagnosis of viral-infectious uveitis. T lymphocytes examination at the inflammatory sites has the potential to become a useful research tool for differentiating viral and non-viral uveitis.

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