scholarly journals Infection of Bovine Immunodeficiency Virus and Bovine Leukemia Virus in Water Buffalo and Cattle Populations in Pakistan

2000 ◽  
Vol 62 (3) ◽  
pp. 329-331 ◽  
Author(s):  
Sothy MEAS ◽  
Junji SETO ◽  
Chihiro SUGIMOTO ◽  
Muhammad BAKHSH ◽  
Muhammad RIAZ ◽  
...  
Keyword(s):  
Bovine Leukemia Virus ◽  
Water Buffalo ◽  
Leukemia Virus ◽  
Bovine Immunodeficiency Virus ◽  
Immunodeficiency Virus

Vertical transmission of bovine leukemia virus and bovine immunodeficiency virus in dairy cattle herds

2002 ◽  
Vol 84 (3) ◽  
pp. 275-282 ◽  
Author(s):  
Sothy Meas ◽  
Tatsufumi Usui ◽  
Kazuhiko Ohashi ◽  
Chihiro Sugimoto ◽  
Misao Onuma
Keyword(s):  
Dairy Cattle ◽  
Vertical Transmission ◽  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Bovine Immunodeficiency Virus ◽  
Immunodeficiency Virus ◽  
Cattle Herds

scholarly journals Seroprevalence of Bovine Immunodeficiency Virus and Bovine Leukemia Virus in Draught Animals in Cambodia.

2000 ◽  
Vol 62 (7) ◽  
pp. 779-781 ◽  
Author(s):  
Sothy MEAS ◽  
Kazuhiko OHASHI ◽  
Sothyra TUM ◽  
Manov CHHIN ◽  
Kuyhor TE ◽  
...  
Keyword(s):  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Bovine Immunodeficiency Virus ◽  
Immunodeficiency Virus ◽  
Draught Animals

scholarly journals Seroprevalence of Bovine Immunodeficiency Virus and Bovine Leukemia Virus in Dairy and Beef Cattle in Hokkaido

2003 ◽  
Vol 65 (2) ◽  
pp. 287-289 ◽  
Author(s):  
Tatsufumi USUI ◽  
Sothy MEAS ◽  
Satoru KONNAI ◽  
Kazuhiko OHASHI ◽  
Misao ONUMA
Keyword(s):  
Beef Cattle ◽  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Bovine Immunodeficiency Virus ◽  
Immunodeficiency Virus

scholarly journals Bovine immunodeficiency virus and bovine leukemia virus and their mixed infection in Iranian Holstein cattle

2010 ◽  
Vol 4 (09) ◽  
pp. 576-579 ◽  
Author(s):  
Gholamreza Nikbakht Brujeni ◽  
Taghi Taghi Poorbazargani ◽  
Susan Nadin-Davis ◽  
Mohammad Tolooie ◽  
Neda Barjesteh
Keyword(s):  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Flow Cytometric Analysis ◽  
Bovine Immunodeficiency Virus ◽  
Holstein Cattle ◽  
T Lymphocyte Subsets ◽  
Flow Cytometric ◽  
Immunodeficiency Virus ◽  
Study Population ◽  
Virus Strains

Introduction: Bovine immunodeficiency virus (BIV) and bovine leukemia virus (BLV) have worldwide distributions, but their prevalences in Iran are unknown. We investigated the presence of infections in Iranian Holstein cattle and determined changes in hematological values for infected animals. Methodology: Nested PCR was used on blood samples from 143 animals Holstein cattle to detect proviral BIV and BLV gag sequences. Flow cytometric analysis was performed using monoclonal antibodies (mAbs) against CD4, CD8, and CD21 bovine T lymphocyte subsets. Results: Proviral BIV and BLV gag sequences were detected in 20.3% and 17% of the animals, respectively. BIV-BLV confection was also detected in 4.2% of the study population but this was not statistically significant. Flow cytometric analysis showed that both BIV-infected cows and non-infected ones had CD4/CD8 ratios of 2.45 and 1.43, respectively, and this difference was significant. BLV infected and non-infected animals had no significant differences in their CD4/CD8 ratio. In comparison to non-infected cattle, those with both BIV and BLV had a significant decrease in their CD4/CD8 ratios (1.5 % vs. 2.3; P = 0.01). Conclusion: This is the first report of BIV and BLV infections in Iran. We found no evidence that infection with one agent predisposed an animal to infection with the other. BIV infection may have a role in decreasing T CD8 counts, but this may depend on the genetics of the cattle and virus strains involved.

scholarly journals Bayesian Estimation of the True Seroprevalence and Risk Factor Analysis of Bovine Leukemia Virus Infection in Pakistan

Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1404
Author(s):  
Ali Sakhawat ◽  
Marzena Rola-Łuszczak ◽  
Zbigniew Osiński ◽  
Nazia Bibi ◽  
Jacek Kuźmak
Keyword(s):  
Risk Factor ◽  
Dairy Cattle ◽  
Bovine Leukemia Virus ◽  
Water Buffalo ◽  
Leukemia Virus ◽  
Credible Interval ◽  
Indigenous Cattle ◽  
Herd Level ◽  
Exotic Breed ◽  
Posterior Estimate

The objective of this study was to determine the true seroprevalence of bovine leukemia virus (BLV) infection in dairy cattle from Pakistan at the animal and herd-level. We tested 1380 dairy cattle from 451 herds and 92 water buffalo. The sera were tested by ELISA and the results were analyzed using Bayesian inference. The median posterior estimate of the herd level true BLV prevalence was 1.4%, with a 95% credible interval (CI) 0.7–3.1, whereas the median posterior estimate of the within-farm true seroprevalence was 3.8% with a 95% CI 2.8–4.8. All 92 sera collected from water buffalo were negative. Several risk factors potentially associated with seropositivity to BLV infections in Pakistan were analyzed using logistic regression model based on calculation of an odds ratio (OR). The study showed an association between seropositivity and medium herd (≥50) size (OR = 23.57, 95% CI: 3.01–103.48). Common housing of indigenous cattle with exotic-breed cattle (OR = 0.67, 95% CI: 06–2.35) or housing indigenous or their crossbred cattle with exotic-breed cattle (OR = 0.95, 95% CI: 0.14–3.01) had no effect on the BLV seroprevalence. Similarly, common housing of cattle and water buffalo was not risk factor for increased BLV seropositivity (OR = 27.10, 95% CI: 0.63–119.34).

scholarly journals Bovine leukemia virus protease: comparison with human T-lymphotropic virus and human immunodeficiency virus proteases

2007 ◽  
Vol 88 (7) ◽  
pp. 2052-2063 ◽  
Author(s):  
Tamás Sperka ◽  
Gabriella Miklóssy ◽  
Yunfeng Tie ◽  
Péter Bagossi ◽  
Gábor Zahuczky ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Animal Model ◽  
Amino Acid ◽  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Model Systems ◽  
T Lymphotropic Virus ◽  
Naturally Occurring ◽  
Immunodeficiency Virus ◽  
Hiv 1

Bovine leukemia virus (BLV) is a valuable model system for understanding human T-lymphotropic virus 1 (HTLV-1); the availability of an infectious BLV clone, together with animal-model systems, will help to explore anti-HTLV-1 strategies. Nevertheless, the specificity and inhibitor sensitivity of the BLV protease (PR) have not been characterized in detail. To facilitate such studies, a molecular model for the enzyme was built. The specificity of the BLV PR was studied with a set of oligopeptides representing naturally occurring cleavage sites in various retroviruses. Unlike HTLV-1 PR, but similar to the human immunodeficiency virus 1 (HIV-1) enzyme, BLV PR was able to hydrolyse the majority of the peptides, mostly at the same position as did their respective host PRs, indicating a broad specificity. When amino acid residues of the BLV PR substrate-binding sites were replaced by equivalent ones of the HIV-1 PR, many substitutions resulted in inactive protein, indicating a great sensitivity to mutations, as observed previously for the HTLV-1 PR. The specificity of the enzyme was studied further by using a series of peptides containing amino acid substitutions in a sequence representing a naturally occurring HTLV-1 PR cleavage site. Also, inhibitors of HIV-1 PR, HTLV-1 PR and other retroviral proteases were tested on the BLV PR. Interestingly, the BLV PR was more susceptible than the HTLV-1 PR to the inhibitors tested. Therefore, despite the specificity differences, in terms of mutation intolerance and inhibitor susceptibility of the PR, BLV and the corresponding animal-model systems may provide good models for testing of PR inhibitors that target HTLV-1.

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