scholarly journals Association between Helicobacter pylori infection and overweight or obesity in a Chinese population

2015 ◽  
Vol 9 (09) ◽  
pp. 945-953 ◽  
Author(s):  
Yan Zhang ◽  
Tingting Du ◽  
Xi Chen ◽  
Xuefeng Yu ◽  
Ling Tu ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Chinese Population ◽  
Statistical Significance ◽  
Positive Association ◽  
Health Examination ◽  
Negative Group ◽  
Positive Group ◽  
Cardiometabolic Disorders ◽  
H Pylori ◽  
Overweight Or Obesity

Introduction: Recent studies found that Helicobacter pylori (H. pylori) infection plays a role in cardiometabolic disorders. The objective of this study was to assess the association between H. pylori infection and overweight or obesity in a Chinese population. Methodology: A cross-sectional analysis using data from the subjects who underwent a health examination between January 2010 and June 2012 in the department of comprehensive medicine was performed. Diagnosis of H. pylori infection was achieved using the carbon urea breath test (14C-UBT). The participants were divided into H. pylori infection-positive group and H. pylori infection-negative group by 14C-UBT. Results: A total of 2,050 subjects were enrolled in the study. The H. pylori infection-positive group had significantly higher body mass index (BMI) levels than did the H. pylori infection-negative group (25.32 vs 24.95, p = 0.008). There was a positive association between H. pylori infection and BMI levels (β = 0.30 ± 0.12, p = 0.015). After additional adjustment for white blood cell count (WBCC), the statistical significance disappeared (β = 0.24 ± 0.12, p = 0.053). Furthermore, a positive association between H. pylori infection and overweight/obesity according to different BMI criteria (BMI ≥ 24, BMI ≥ 23) was found. However, the association between H. pylori infection and obesity was consistently significant only based on the Asian criteria (BMI ≥ 27.5), but not significant based on the Chinese criteria (BMI ≥ 28). Conclusion: H. pylori infection was significantly and positively associated with overweight/obesity in a Chinese population.

DETERMINATION OF HELICOBACTER PYLORI CAGA GENE AND VACA GENOTYPES IN PATIENTS WITH GASTRIC CANCER

2013 ◽  
pp. 118-125
Author(s):  
Quy Hung Le ◽  
Thi Minh Thi Ha
Keyword(s):  
Gastric Cancer ◽  
Helicobacter Pylori ◽  
Signet Ring Cell ◽  
Tubular Adenocarcinoma ◽  
Negative Group ◽  
Caga Gene ◽  
Positive Group ◽  
Histopathological Classification ◽  
Vaca Gene ◽  
H Pylori

Background: H. pylori is the first cause of gastric cancer (GC). However, the role of cagA gene and vacA gene in GC is still controversial. This study is aimed at determining the rates of H. pylori infection, cagA gene, vacA genotypes in patients with GC; and evaluating the relationship between cagA gene, vacA genotypes and endoscopic and histopathological features of gastric cancer. Patients and methods: Fifty eight GC patients and one hundred and sixteen non-GC patients (controls) were enrolled. Infection of H. pylori was determined by PCR. cagA gene and vacA genotypes were determined by Multiplex PCR. Results: The rate of H. pylori was found in 55.2% in GC group. The rate of cagA gene and vacA gene in GC patients H. pylori positive were found in 78.1% and 100%, respectively. vac A genotypes s1/m1, s1/m2 and s1/m1m2 were found in 34.4%; 50% and 15.6%, respectively. The risk of GC of cagA positive group was higher than cagA negative group, with OR = 4,5; 95%CI = 1.6-12.2. The risk of GC of vacA s1/m1, cagA positive group was higher than vacA s1/m1, cagA negative group, OR = 7.1; 95%CI = 1.4-36. A statistically significative difference of rate of cagA positive was found between Borrmann III/IV group (100%) and Borrmann I/II group (46.2%). A statistically significative difference of rate of cagA positive was found between the tubular adenocarcinoma group (100%) and signet-ring cell carcinoma (44.4%, p = 0,002), and mucinous adenocarcinoma (50%, p =0,024). Conclusion: Gene cagA and vacA s1/m1 genotype were both risk factors in GC. A significative differences of rate of cagA positive were found between Borrmann groups, and between groups of WHO histopathological classification. Key words: cagA gene and vacA genotype, Helicobacter pylori, gastric cancer

scholarly journals The association between Helicobacter pylori infection and food intolerance in western China

2020 ◽  
Author(s):  
Ying Liu ◽  
Dongyu Li ◽  
Ning Xiao ◽  
Yuping Liu ◽  
Ping Shuai
Keyword(s):  
Helicobacter Pylori ◽  
Infection Rate ◽  
Multivariate Logistic Regression Analysis ◽  
Food Intolerance ◽  
Western China ◽  
Negative Group ◽  
Positive Group ◽  
Total Food ◽  
Specific Igg ◽  
H Pylori

Abstract IntroductionHelicobacter pylori (H. pylori) has been found to be associated with extragastrointestinal diseases while the studies on its association with food intolerance are rare. In our study, we tried to explore the relationship between H. pylori infection and food intolerance.MethodsWe retrospectively analyzed the physical examination data of 21,822 subjects from February 2014 to December 2018 in this study. H. pylori infection was determined by 13C urea breath test. The food specific IgG in serum were detected to assess the food tolerance status of egg, milk and wheat.ResultsThe total infection rate of H. pylori was 39.3% and the total food intolerance rates of egg, milk and wheat were 25.2%, 9.0% and 4.7%, respectively. The infection rate of H. pylori was higher in males than that in females, while the intolerance rates were lower in males than that in females. The infection rates of H. pylori increased with age except for people over sixty, while the intolerance rates decreased. In H. pylori positive group, the intolerance rates were lower than that in the H. pylori negative group. At the same level of food intolerance, the intolerance rates in H. pylori positive group were lower than that in H. pylori negative group. Multivariate Logistic regression analysis showed that H. pylori infection was negatively correlated with intolerance of egg, milk and wheat (OR value of egg 0.844–0.873, milk 0.741–0.751, wheat 0.762–0.801).ConclusionsH. pylori infection was negatively correlated with intolerance of egg, milk and wheat in Sichuan, western China.

scholarly journals 1223. Impact of Helicobacter pylori Infection on Duodenal Microbial Community Structures and Microbial Metabolic Pathways

2020 ◽  
Vol 7 (Supplement_1) ◽  
pp. S632-S632
Author(s):  
Tadashi Maeda ◽  
hiroaki zai ◽  
Yuto Fukui ◽  
yoshihumi katou ◽  
eri kumade ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Metabolic Pathways ◽  
Amplicon Sequencing ◽  
Helicobacter Pylori Infection ◽  
Rrna Gene ◽  
Negative Group ◽  
Positive Group ◽  
Principal Factors ◽  
Gastric Cancer Screening ◽  
H Pylori

Abstract Background Recent reports suggest that Helicobacter pylori infection causes extragastric diseases. However, the onset mechanisms of these diseases have not been fully elucidated, and the factors involved in the onset of these extragastric diseases remain obscure. Methods Forty-seven (20 male, 27 female) subjects who underwent gastric cancer screening were enrolled. Aspirated duodenal fluid samples were collected from the descending duodenum. Samples were analyzed by 16S rRNA gene amplicon sequencing to investigate whether the duodenal microbiota and microbial biofunctions were affected by H. pylori infection. Results Thirteen subjects were H. pylori positive while 34 were negative. We observed 1404 bacterial operational taxonomic units from 23 phyla and 253 genera. In the H. pylori positive group, we observed higher abundance of Proteobacteria and lower abundance of Actinobacteria and TM7 than in the H. pylori negative group. The abundance of 10 genera differed significantly between the H. pylori positive and negative groups. Aspects of microbiota in the H. pylori positive group were significantly influenced by 12 taxa primarily belonging to Gammaproteobacteria, compared with those in the H. pylori negative group. Microbial functional annotation collated using the Kyoto Encyclopedia of Genes and Genomes Orthology database showed that 12 microbial metabolic pathways were significantly affected by H. pylori infection. Conclusion 1. pylori infection disrupted the normal bacterial communities in the duodenum and changed aspects of the commensal microbial functions primarily by upregulating the metabolic pathways. This may be one of the principal factors in the development of extragastric diseases. Disclosures All Authors: No reported disclosures

Linkage between H. pylori Infection and TNF-α polymorphism in The Pregnant Women

2018 ◽  
Vol 9 (SPL1) ◽  
Author(s):  
Ghaidaa Raheem Lateef ◽  
Azhar Omaran Al-Thahab
Keyword(s):  
Pregnant Women ◽  
Serum Antibody ◽  
Rapid Test ◽  
Negative Group ◽  
Positive Group ◽  
Gynecology And Obstetrics ◽  
Tnf Α ◽  
Significant Difference ◽  
Pcr Products ◽  
H Pylori

A study was performed on 100 pregnant women in the outpatient department of gynecology and obstetrics of Maternity and Children Hospital in Al-Diwaniya City during the period between (March to September 2016). One hundred blood samples (50 for patients and 50 for control) were collected under the supervision of the treating gynecologist. The detection of Helicobacter. pylori was done by the use of the serum antibody Rapid test. The results showed that 50 (100%) were positive and 50 (100%) were negative for H. pylori in above method.All blood of patients and control samples were used for the extraction of genomic DNA,where the 107 bp PCR product size. Genotyping of the TNF-α-308 SNP (G/A)was performed by restriction fragment length polymorphism PCR (RFLP-PCR). PCR products were digested with restr NcoI iction enzyme. Individuals with the TNF-α-308(GG) homozygote produced digested DNA bands at 80,and 20 bp bp. A heterozygous genotype ofTNF-α-308 (GA)produced 107 bp,80 bp,and 20 bp bands. Individuals with the TNF-α-308 (AA) homozygote genotype had no amplicon digested and generated only one band of 107 bp. There was a significant difference in the frequency of the TNF-α-308(GG)genotype between H. pylori positive group and H. pylori negative group(72%,78% respectively). Also for GA genotype,there was a significant difference between H. pylori positive group and H. pylori negative group(24%,18% respectively). Concerning the frequency of the TNF-α-308 (AA)genotype between H. pylori positive group and H. pylori negative group,there was no significant difference between the two groups.

scholarly journals Diversity of 3′ variable region of cagA gene in Helicobacter pylori strains isolated from Chinese population

Gut Pathogens ◽  
2021 ◽  
Vol 13 (1) ◽  
Author(s):  
Zhijing Xue ◽  
Yuanhai You ◽  
Lihua He ◽  
Yanan Gong ◽  
Lu Sun ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Helicobacter Pylori ◽  
Amino Acid ◽  
Chinese Population ◽  
Statistical Difference ◽  
Variable Region ◽  
Repeat Region ◽  
Geographical Regions ◽  
H Pylori ◽  
Epiya Motifs

Abstract Background The cytotoxin-associated gene A (cagA) is one of the most important virulence factors of Helicobacter pylori (H. pylori). There is a highly polymorphic Glu-Pro-Ile-Tyr-Ala (EPIYA) repeat region in the C-terminal of CagA protein. This repeat region is thought to play an important role in the pathogenesis of gastrointestinal diseases. The aim of this study was to investigate the diversity of cagA 3′ variable region and the amino acid polymorphisms in the EPIYA segments of the CagA C-terminal region of H. pylori, and their association with gastroduodenal diseases. Methods A total of 515 H. pylori strains from patients in 14 different geographical regions of China were collected. The genomic DNA from each strain was extracted and the cagA 3′ variable region was amplified by polymerase chain reaction (PCR). The PCR products were sequenced and analyzed using MEGA 7.0 software. Results A total of 503 (97.7%) H. pylori strains were cagA-positive and 1,587 EPIYA motifs were identified, including 12 types of EPIYA or EPIYA-like sequences. In addition to the four reported major segments, several rare segments (e.g., B′, B″ and D′) were defined and 20 different sequence types (e.g., ABD, ABC) were found in our study. A total of 481 (95.6%) strains carried the East Asian type CagA, and the ABD subtypes were most prevalent (82.1%). Only 22 strains carried the Western type CagA, which included AC, ABC, ABCC and ABCCCC subtypes. The CagA-ABD subtype had statistical difference in different geographical regions (P = 0.006). There were seven amino acid polymorphisms in the sequences surrounding the EPIYA motifs, among which amino acids 893 and 894 had a statistical difference with gastric cancer (P = 0.004). Conclusions In this study, 503 CagA sequences were studied and analyzed in depth. In Chinese population, most H. pylori strains were of the CagA-ABD subtype and its presence was associated with gastroduodenal diseases. Amino acid polymorphisms at residues 893 and 894 flanking the EPIYA motifs had a statistically significant association with gastric cancer.

scholarly journals Different dose of new generation proton pump inhibitors for the treatment of Helicobacter pylori infection: A meta-analysis

2021 ◽  
Vol 35 ◽  
pp. 205873842110303
Author(s):  
Wenwen Gao ◽  
Xiang Zhang ◽  
Yanhui Yin ◽  
Shuwen Yu ◽  
Lu Wang
Keyword(s):  
Helicobacter Pylori ◽  
Proton Pump Inhibitors ◽  
Proton Pump ◽  
Eradication Rate ◽  
Standard Dose ◽  
Statistical Significance ◽  
High Dose ◽  
Clarithromycin Resistance ◽  
H Pylori ◽  
New Generation

The evidence on whether high-dose new generation proton pump inhibitors (PPIs) including rabeprazole and esomeprazole achieve a higher eradication rate of Helicobacter pylori has not been assessed. The primary comparison was eradication and adverse events (AEs) rate of standard (esomeprazole 20 mg bid, rabeprazole 10 mg bid) versus high-dose (esomeprazole 40 mg bid, rabeprazole 20 mg bid) PPIs. Sub-analyses were performed to evaluate the eradication rate between Asians and Caucasians, clarithromycin-resistance (CAM-R) strains, and clarithromycin-sensitivity (CAM-S) strains of different dose PPIs. We conducted a literature search for randomized controlled trials comparing high-with standard-dose esomeprazole and rabeprazole for H. pylori eradication and AEs. A total of 12 trials with 2237 patients were included. The eradication rate of high-dose PPIs was not significantly superior to standard-dose PPIs regimens: 85.3% versus 84.2%, OR 1.09 (0.86–1.37), P = 0.47. The high dose induced more AEs than those of the standard dose, but didn’t reach statistical significance (OR 1.25, 95% CI: 0.99–1.56, P = 0.06). Subgroup analysis showed that the difference in eradication rate of PPIs between high- and standard-dose groups were not statistically significant both in Asians (OR 0.99, 95% CI 0.75–1.32, P = 0.97) and Caucasians (OR 1.27, 95% CI 0.84–1.92, P = 0.26). Furthermore, there were similar eradication rates in CAM-S (OR 1.2; 95% CI 0.58–2.5; P = 0.63) and CAM-R strains (OR 1.08; 95% CI 0.45–2.56; P = 0.87) between the standard-and high-dose groups. High and standard dosages of new generation of the PPIs showed similar H. pylori eradication rates and AEs as well as between Asian versus Caucasian populations, with or without clarithromycin-resistance. However, further studies are needed to confirm.

scholarly journals Diversity of 3’ variable region of cagA gene in Helicobacter pylori strains isolated from Chinese population

2021 ◽  
Author(s):  
Zhijing Xue ◽  
Yuanhai You ◽  
Lihua He ◽  
Yanan Gong ◽  
Lu Sun ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Helicobacter Pylori ◽  
Amino Acid ◽  
Chinese Population ◽  
Statistical Difference ◽  
Variable Region ◽  
Repeat Region ◽  
Geographical Regions ◽  
H Pylori ◽  
Epiya Motifs

Abstract Background: The cytotoxin-associated gene A (cagA) is one of the most important virulence factors of Helicobacter pylori (H. pylori). There is a highly polymorphic Glu-Pro-Ile-Tyr-Ala (EPIYA) repeat region in the C-terminal of CagA protein. This repeat region is thought to play an important role in the pathogenesis of gastrointestinal diseases. The aim of this study was to investigate the diversity of cagA 3’ variable region and the amino acid polymorphisms in the EPIYA segments of the CagA C-terminal region of H. pylori, and their association with gastroduodenal diseases.Methods: A total of 515 H. pylori strains from patients in 14 different geographical regions of China were collected. The genomic DNA from each strain was extracted and the cagA 3’ variable region was amplified by polymerase chain reaction (PCR). The PCR products were sequenced and analyzed using MEGA 7.0 software.Results: A total of 503 (97.7%) H. pylori strains were cagA-positive and 1,587 EPIYA motifs were identified, including 12 types of EPIYA or EPIYA-like sequences. In addition to the four reported major segments, several rare segments (e.g., B’, B’’ and D’) were defined and 20 different sequence types (e.g., ABD, ABC) were found in our study. A total of 481 (95.6%) strains carried the East Asian type CagA, and the ABD subtypes were most prevalent (82.1%). Only 22 strains carried the Western type CagA, which include AC, ABC, ABCC and ABCCCC subtypes. The CagA-ABD subtype had statistical difference in different geographic regions (P = 0.006). There are seven amino acid polymorphisms in the sequences surrounding the EPIYA motifs, among which amino acid residue 893 and 894 had a statistical difference with gastric cancer (P = 0.004).Conclusions: In this study, 503 CagA sequences was studied and analyzed in depth. In Chinese population, most H. pylori strains are of the CagA-ABD subtype and its presence was associated with gastroduodenal diseases. Amino acid polymorphisms at residue 893 and 894 flanking the EPIYA motif had a statistically significant association with gastric cancer.

scholarly journals Seroprevalence of Helicobacter pylori in patients with colon adenomas in a brazilian university hospital

2009 ◽  
Vol 46 (2) ◽  
pp. 97-101 ◽  
Author(s):  
Abadia Gilda Buso ◽  
Haroldo Luis Oliva Gomes Rocha ◽  
Débora Miranda Diogo ◽  
Priscila Miranda Diogo ◽  
Augusto Diogo-Filho
Keyword(s):  
Helicobacter Pylori ◽  
Solid Phase ◽  
Positive Association ◽  
University Hospital ◽  
Igg Antibody ◽  
Chi Square ◽  
Colon Adenomas ◽  
Colonic Adenomas ◽  
Significant Difference ◽  
H Pylori

CONTEXT: The association between Helicobacter pylori infection and colon neoplasia has been the subject of recent investigations which have produced controversial results. OBJECTIVE: To evaluate the prevalence of H. pylori infection in patients with colonic adenomas and also in patients whose colonoscopy exams were normal. METHODS: After colonoscopy, the individuals were distributed into two groups: patients with colon adenomas (cases) and patients whose colons were normal (controls). The groups were similar regarding age and gender. The individuals of both groups were subjected to a dosage of IgG antibody against H. pylori. The dosage was applied according to the solid phase, chemiluminescent immunometric assay. The chi-square test was used to analyze the data. RESULTS: There were 30 men and 64 women in each group (94 cases and 94 controls). The mean age of the cases was 59.79 ± 12.25 years and that of the controls was 58.98 ± 11.55 years. The H. pylori serology was positive for 66 (70.21%) of the cases and for 51 (54.25%) of the controls. There was a significant difference (P = 0.024). The odds ratio was 1.98 (CI 95%, 0.82-3.15). The prevalence of H. pylori in cases and controls according to gender, histological type and location of the colon lesions showed a significant difference only among women (P = 0.03), among patients with tubular adenomas (P = 0.03), and in those with distal adenomas (P = 0.038). CONCLUSION: There is a positive association between H. pylori infection and colonic adenomas. This association is more evident in women, especially for tubular adenomas and distal colonic location.

Intranasal Helicobacter pylori infection in patients with chronic rhinosinusitis with polyposis

2018 ◽  
Vol 132 (9) ◽  
pp. 816-821 ◽  
Author(s):  
N Siupsinskiene ◽  
I Katutiene ◽  
V Jonikiene ◽  
D Janciauskas ◽  
S Vaitkus
Keyword(s):  
Helicobacter Pylori ◽  
Chronic Rhinosinusitis ◽  
Laryngopharyngeal Reflux ◽  
Statistical Significance ◽  
Control Group ◽  
Clinical Group ◽  
Posterior Commissure ◽  
Nasal Tissue ◽  
H Pylori ◽  
Nasal Biopsy

AbstractObjectiveTo determine the prevalence of Helicobacter pylori infection in nasal biopsy specimens from patients with chronic rhinosinusitis with polyposis versus control patients, and to assess the correlations between H pylori infection identified in the nasal tissue and patients’ sociodemographic data and reflux-related symptoms and signs.MethodsNasal biopsy samples were taken from 75 adult patients who underwent nasal surgery for chronic rhinosinusitis with polyposis (clinical group, n = 45) and a deviated septum (control group, n = 30). H pylori infection was identified using histochemical and rapid urease tests.ResultsThe prevalence of intranasal H pylori infection was significantly higher in the clinical group (28.9 per cent) compared to the control group (3.3 per cent) (p = 0.005). A significant yet weak association was found between positive H pylori status and laryngopharyngeal reflux related hypertrophy of the posterior commissure of the larynx. No other correlations reached statistical significance.ConclusionH pylori infection is potentially related to chronic rhinosinusitis with polyposis. Further research is needed to clarify the role of H pylori as a risk factor for the development of sinonasal diseases and to examine its link with laryngopharyngeal reflux.

scholarly journals Association Between Vitamin D Receptor Gene Polymorphisms and Helicobacter Pylori Infection

2020 ◽  
Vol 10 (1) ◽  
pp. 8-14
Author(s):  
Amal A. Mohamed ◽  
Shorouk Moussa ◽  
Mahmoud M. Shaheen ◽  
Sherief Abd-Elsalam ◽  
Rehab Ahmed ◽  
...  
Keyword(s):  
Vitamin D ◽  
Helicobacter Pylori ◽  
Vitamin D Receptor ◽  
Gene Polymorphisms ◽  
Gastrointestinal Symptoms ◽  
Upper Gastrointestinal Endoscopy ◽  
Positive Group ◽  
Biopsy Specimens ◽  
H Pylori ◽  
Upper Gastrointestinal

Background & Aims: Human genetic polymorphism has been reported in the susceptibility and clinical development of infection. In this regard, this study aimed to investigate the link between Vitamin D Receptor (VDR) gene polymorphism and H. pylori infection. Materials and Methods: This cross-sectional study was conducted on 224 adult patients with upper gastrointestinal symptoms who underwent an upper gastrointestinal endoscopy between July 2017 and May 2019 in two major university hospitals. All patients were evaluated for helicobacter pylori infection. Two gastric antral biopsy specimens were taken from each patient. One of those Biopsy specimens was evaluated for histopathology examination and the other one was immersed in a saline solution ready for genomic DNA extraction. Results: There were statistically significant differences between different genotypes of VDR rs7975232 polymorphism between H. pylori infected and non-infected groups (CC was higher in H. pylori negative and AC and AA were the most common in H. pylori positive group). There were statistical differences between different genotypes of VDR rs2228570 between H. pylori infected and non-infected groups (TT was higher in H. pylori negative and CT and CC were the most common in H. pylori positive group). Regarding VDR rs 7975232 gene polymorphisms; the (A) allele was significantly higher H. pylori infected, while (C) allele was significantly higher in uninfected patients. Regarding VDR rs 2228570 gene polymorphisms; the (C) allele was significantly higher H. pylori infected, while (T) allele was significantly higher in uninfected patients. Conclusion: There is a possible association between the FokI and Apal VDR polymorphism and H. pylori infection.

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