DETERMINATION OF HELICOBACTER PYLORI CAGA GENE AND VACA GENOTYPES IN PATIENTS WITH GASTRIC CANCER

2013 ◽  
pp. 118-125
Author(s):  
Quy Hung Le ◽  
Thi Minh Thi Ha
Keyword(s):  
Gastric Cancer ◽  
Helicobacter Pylori ◽  
Signet Ring Cell ◽  
Tubular Adenocarcinoma ◽  
Negative Group ◽  
Caga Gene ◽  
Positive Group ◽  
Histopathological Classification ◽  
Vaca Gene ◽  
H Pylori

Background: H. pylori is the first cause of gastric cancer (GC). However, the role of cagA gene and vacA gene in GC is still controversial. This study is aimed at determining the rates of H. pylori infection, cagA gene, vacA genotypes in patients with GC; and evaluating the relationship between cagA gene, vacA genotypes and endoscopic and histopathological features of gastric cancer. Patients and methods: Fifty eight GC patients and one hundred and sixteen non-GC patients (controls) were enrolled. Infection of H. pylori was determined by PCR. cagA gene and vacA genotypes were determined by Multiplex PCR. Results: The rate of H. pylori was found in 55.2% in GC group. The rate of cagA gene and vacA gene in GC patients H. pylori positive were found in 78.1% and 100%, respectively. vac A genotypes s1/m1, s1/m2 and s1/m1m2 were found in 34.4%; 50% and 15.6%, respectively. The risk of GC of cagA positive group was higher than cagA negative group, with OR = 4,5; 95%CI = 1.6-12.2. The risk of GC of vacA s1/m1, cagA positive group was higher than vacA s1/m1, cagA negative group, OR = 7.1; 95%CI = 1.4-36. A statistically significative difference of rate of cagA positive was found between Borrmann III/IV group (100%) and Borrmann I/II group (46.2%). A statistically significative difference of rate of cagA positive was found between the tubular adenocarcinoma group (100%) and signet-ring cell carcinoma (44.4%, p = 0,002), and mucinous adenocarcinoma (50%, p =0,024). Conclusion: Gene cagA and vacA s1/m1 genotype were both risk factors in GC. A significative differences of rate of cagA positive were found between Borrmann groups, and between groups of WHO histopathological classification. Key words: cagA gene and vacA genotype, Helicobacter pylori, gastric cancer

scholarly journals Incidence of gastric cancer, its subtypes, and correlation with Helicobacter Pylori

2013 ◽  
Vol 3 (5) ◽  
pp. 403-407
Author(s):  
Shiva Raj KC ◽  
GL Amatya ◽  
A Lakhey ◽  
S Basnet ◽  
G Aryal
Keyword(s):  
Gastric Cancer ◽  
Helicobacter Pylori ◽  
Demographic Data ◽  
College Teaching ◽  
Study Data ◽  
Upper Gastrointestinal Endoscopy ◽  
Tubular Adenocarcinoma ◽  
Common Cause ◽  
Medical College ◽  
H Pylori

Background: Gastric cancer is the fourth most commonly diagnosed cancer and the second most common cause of cancer related death worldwide. It is the common cause of cancer related death in Nepal. Helicobacter Pylori has been classified as a definite carcinogen along with other factors. The aim of this study was to fi nd the incidence of gastric cancer among the patients undergoing upper gastroscopy, its various subtypes and association with Helicobacter Pylori. Materials and Methods: This is a retrospective and prospective study carried out at GRP Polyclinic and Kist Medical College Teaching Hospital. All the patients undergoing upper gastrointestinal endoscopy were included in this study. Data of all the gastric endoscopic biopsies done from June 2011 to January 2013 were collected and analyzed. All the biopsy specimens were processed routinely in histopathology laboratory. Specimens showing carcinoma were enrolled in this study and all the relevant demographic data were collected. Results: Out of 3395 biopsy cases; 49 cases (1.44%) were diagnosed as adenocarcinoma stomach. The overall mean age for carcinoma was 47.6 years with a mild male preponderance. Thirty cases (61.2%) were of intestinal type, (n=11; 22.4%) were of diffuse type and (n=8; 16.3%) were mixed type of adenocarcinoma. According to WHO classifi cation the most common subtype was tubular adenocarcinoma (n=35; 71.5%) followed by signet ring type (11 cases; 22.4%). Out of 49 cases of adenocarcinoma stomach 39 cases (79.5%) were Helicobacter Pylori positive. Conclusion: This study shows that gastric carcinoma is a male predominant neoplasm usually of old age but can occur at younger ages. It predominantly occurs in Helicobacter Pylori infected patients and H. Pylori eradication will help to decrease the incidence rate and mortality of stomach cancer. Journal of Pathology of Nepal (2013) Vol. 3, No.1, Issue 5, 403-407 DOI: http://dx.doi.org/10.3126/jpn.v3i5.7869

scholarly journals Whole Genome Sequencing Reveals Virulence Potentials of Helicobacter pylori Strain KE21 Isolated from a Kenyan Patient with Gastric Signet Ring Cell Carcinoma

Toxins ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 556
Author(s):  
Catherine Mwangi ◽  
Stephen Njoroge ◽  
Evariste Tshibangu-Kabamba ◽  
Zahir Moloo ◽  
Allan Rajula ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Helicobacter Pylori ◽  
Illumina Miseq ◽  
Signet Ring Cell Carcinoma ◽  
Signet Ring Cell ◽  
Oncogenic Pathways ◽  
Long Reads ◽  
Oxford Nanopore ◽  
Study Population ◽  
H Pylori

Helicobacter pylori (H.pylori) infection is etiologically associated with severe diseases including gastric cancer; but its pathogenicity is deeply shaped by the exceptional genomic diversification and geographic variation of the species. The clinical relevance of strains colonizing Africa is still debated. This study aimed to explore genomic features and virulence potentials of H. pylori KE21, a typical African strain isolated from a native Kenyan patient diagnosed with a gastric cancer. A high-quality circular genome assembly of 1,648,327 bp (1590 genes) obtained as a hybrid of Illumina Miseq short reads and Oxford Nanopore MinION long reads, clustered within hpAfrica1 population. This genome revealed a virulome and a mobilome encoding more than hundred features potentiating a successful colonization, persistent infection, and enhanced disease pathogenesis. Furthermore, through an experimental infection of gastric epithelial cell lines, strain KE21 showed the ability to promote interleukin-8 production and to induce cellular alterations resulting from the injection of a functional CagA oncogene protein into the cells. This study shows that strain KE21 is potentially virulent and can trigger oncogenic pathways in gastric epithelial cells. Expended genomic and clinical explorations are required to evaluate the epidemiological importance of H. pylori infection and its putative complications in the study population.

scholarly journals Prevalence of Helicobacter pylori vacA, cagA, cagE1, cagE2, dupA and oipA Genotypes in Patients With Gastrointestinal Diseases

2020 ◽  
Author(s):  
Hossein Masoumi Asl ◽  
Ali Badamchi ◽  
Shima Javadinia ◽  
Siamak Khaleghi ◽  
Leila Tehraninia ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Helicobacter Pylori ◽  
Gastrointestinal Diseases ◽  
Cancer Group ◽  
Urease Test ◽  
High Prevalence ◽  
Vaca Gene ◽  
H Pylori ◽  
Positive Results ◽  
Gastroenterology Unit

Helicobacter pylori (H. pylori) is a bacterium that resides in the human stomach, which is associated with gastroduodenal diseases. We investigate the prevalence of cagA, vacA, oipA, cagE1, cagE2 and dupA genotypes in H. pylori isolated from patients with Gastric ulcer, duodenal ulcer, and Gastric Cancer. Collected 74 samples from the Gastroenterology Unit of the Rasool Akram Hospital were included in this study. Gastric disorders were identified by endoscopy .gastric cancer was further confirmed by histopathology. H. pylori were detected by the urease test. Subsequently, DNA was extracted from gastric tissue of the subjects with the CLO-test yielded positive results. In general, 74 patients with a mean age of 53.45 years (Range 22 to 86-year-old), including 45 men and 29 women, were studied. Among 74 H. pylori-positive patients, 70 (94.5%) patients were positive for the cagA gene. About 95.8% (23/24) of the patients with gastric carcinoma were dupA positive and VacA gene (91.8%). The oipA genotype was detected in 71 (96%) of H.pylori positive samples. This gene was more common in patients with gastritis rather than cancer group. Also, 97.2% of 74 H. pylori isolates were cagE2-positive. In 25 patients with PUD, the occurrence percent of cagA+/VacA+, cagA+/Vac- , cagA- /VacA+ and cagA- /VaxA- genotypes were found 80%, 12%, 4.2% and 4.2 respectively. The results of the present study suggest that a high prevalence of virulent factors could contribute to the risk of developing gastroduodenal diseases.

scholarly journals Association between Helicobacter pylori infection and overweight or obesity in a Chinese population

2015 ◽  
Vol 9 (09) ◽  
pp. 945-953 ◽  
Author(s):  
Yan Zhang ◽  
Tingting Du ◽  
Xi Chen ◽  
Xuefeng Yu ◽  
Ling Tu ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Chinese Population ◽  
Statistical Significance ◽  
Positive Association ◽  
Health Examination ◽  
Negative Group ◽  
Positive Group ◽  
Cardiometabolic Disorders ◽  
H Pylori ◽  
Overweight Or Obesity

Introduction: Recent studies found that Helicobacter pylori (H. pylori) infection plays a role in cardiometabolic disorders. The objective of this study was to assess the association between H. pylori infection and overweight or obesity in a Chinese population. Methodology: A cross-sectional analysis using data from the subjects who underwent a health examination between January 2010 and June 2012 in the department of comprehensive medicine was performed. Diagnosis of H. pylori infection was achieved using the carbon urea breath test (14C-UBT). The participants were divided into H. pylori infection-positive group and H. pylori infection-negative group by 14C-UBT. Results: A total of 2,050 subjects were enrolled in the study. The H. pylori infection-positive group had significantly higher body mass index (BMI) levels than did the H. pylori infection-negative group (25.32 vs 24.95, p = 0.008). There was a positive association between H. pylori infection and BMI levels (β = 0.30 ± 0.12, p = 0.015). After additional adjustment for white blood cell count (WBCC), the statistical significance disappeared (β = 0.24 ± 0.12, p = 0.053). Furthermore, a positive association between H. pylori infection and overweight/obesity according to different BMI criteria (BMI ≥ 24, BMI ≥ 23) was found. However, the association between H. pylori infection and obesity was consistently significant only based on the Asian criteria (BMI ≥ 27.5), but not significant based on the Chinese criteria (BMI ≥ 28). Conclusion: H. pylori infection was significantly and positively associated with overweight/obesity in a Chinese population.

scholarly journals The association between Helicobacter pylori infection and food intolerance in western China

2020 ◽  
Author(s):  
Ying Liu ◽  
Dongyu Li ◽  
Ning Xiao ◽  
Yuping Liu ◽  
Ping Shuai
Keyword(s):  
Helicobacter Pylori ◽  
Infection Rate ◽  
Multivariate Logistic Regression Analysis ◽  
Food Intolerance ◽  
Western China ◽  
Negative Group ◽  
Positive Group ◽  
Total Food ◽  
Specific Igg ◽  
H Pylori

Abstract IntroductionHelicobacter pylori (H. pylori) has been found to be associated with extragastrointestinal diseases while the studies on its association with food intolerance are rare. In our study, we tried to explore the relationship between H. pylori infection and food intolerance.MethodsWe retrospectively analyzed the physical examination data of 21,822 subjects from February 2014 to December 2018 in this study. H. pylori infection was determined by 13C urea breath test. The food specific IgG in serum were detected to assess the food tolerance status of egg, milk and wheat.ResultsThe total infection rate of H. pylori was 39.3% and the total food intolerance rates of egg, milk and wheat were 25.2%, 9.0% and 4.7%, respectively. The infection rate of H. pylori was higher in males than that in females, while the intolerance rates were lower in males than that in females. The infection rates of H. pylori increased with age except for people over sixty, while the intolerance rates decreased. In H. pylori positive group, the intolerance rates were lower than that in the H. pylori negative group. At the same level of food intolerance, the intolerance rates in H. pylori positive group were lower than that in H. pylori negative group. Multivariate Logistic regression analysis showed that H. pylori infection was negatively correlated with intolerance of egg, milk and wheat (OR value of egg 0.844–0.873, milk 0.741–0.751, wheat 0.762–0.801).ConclusionsH. pylori infection was negatively correlated with intolerance of egg, milk and wheat in Sichuan, western China.

scholarly journals Clinicopathological Characteristics and Endoscopic Features of Early Gastric Cancers Diagnosed After Helicobacter pylori Eradication: A Retrospective Study

2020 ◽  
Author(s):  
Hideki Ishibashi ◽  
Hidetoshi Takedatsu ◽  
Taro Tanabe ◽  
So Imakiire ◽  
Hiroki Matsuoka ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Helicobacter Pylori ◽  
Risk Factor ◽  
Retrospective Study ◽  
Clinicopathological Characteristics ◽  
Depressed Type ◽  
Helicobacter Pylori Eradication ◽  
Positive Group ◽  
H Pylori

Abstract Background: Helicobacter pylori (H. pylori) infection is an important risk factor for developing gastric cancer. However, even after H. pylori eradication, early gastric cancer (EGC) can develop. We elucidated the characteristics of EGCs diagnosed after H. pylori eradication. Methods: Thirty-six EGCs in 32 patients diagnosed after H. pylori eradication were defined as the eradication group (H. pylori-EG). The clinicopathological and endoscopic features were compared with those of 156 EGCs in 140 patients in the H. pylori-positive group (H. pylori-PG). Twenty-nine EGC lesions in the H. pylori-EG were further divided into two subgroups: the first included six lesions of no to mild atrophic mucosa around the EGC, and the second included 23 lesions of moderate to severe atrophic mucosa around the EGC. We compared them between the two subgroups. Results: Endoscopic features of EGCs in the H. pylori-EG were characterized as small (P = 0.049) and of the depressed type (P = 0.022) compared with those in the H. pylori-PG. EGCs in the H. pylori-EG were detected in the upper region of the stomach more frequently than those in the H. pylori-PG (P = 0.002). Submucosal ECGs in the H. pylori-EG were more likely to be seen in the no to mild atrophic mucosa subgroup (4/6, 67%) compared with the moderate to severe atrophic gastric mucosa subgroup (1/23, 4%) (P = 0.003). Conclusions: Careful follow-up endoscopies are necessary after H. pylori eradication.

scholarly journals Seroprevalence of Helicobacter pylori infection and gastric mucosal atrophy in Bhutan, a country with a high prevalence of gastric cancer

2013 ◽  
Vol 62 (10) ◽  
pp. 1571-1578 ◽  
Author(s):  
Seiji Shiota ◽  
Varocha Mahachai ◽  
Ratha-korn Vilaichone ◽  
Thawee Ratanachu-ek ◽  
Lotay Tshering ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Helicobacter Pylori ◽  
Negative Group ◽  
Mucosal Atrophy ◽  
Gastric Mucosal Atrophy ◽  
High Prevalence ◽  
Group A ◽  
H Pylori ◽  
Group B ◽  
Group D

Gastric cancer is the second leading cause of cancer-related mortality in the world. Recently, serum Helicobacter pylori antibodies and pepsinogen (PG) have been used for gastric cancer screening. The incidence of gastric cancer in Bhutan is reported to be quite high compared with that in neighbouring countries. In this study, 381 subjects from three areas of Bhutan were assessed for gastric mucosal atrophy and serological parameters. Anti-H. pylori IgG, PG I, PG II and cytotoxin-associated gene A (CagA) antibodies were measured using ELISA. Subjects were classified into four groups according to H. pylori and PG seropositivity: Group A (H. pylori-negative/PG-negative), Group B (H. pylori-positive/PG-negative), Group C (H. pylori-positive/PG-positive) and Group D (H. pylori-negative/PG-positive). The prevalence of H. pylori in the 381 subjects was 71.1 % (271/381), with high infection rates found in rural areas. The PG I/II ratio was significantly inversely correlated with the atrophy score in the antrum and the corpus (P<0.001). Multivariate analysis showed that the PG status was significantly associated with the presence of atrophy in the corpus. The prevalence of the PG-positive status was significantly higher among H. pylori-positive subjects than among H. pylori-negative subjects (P<0.001). Based on the ABC method, Group B was the most dominant, followed by Group A, Group C and Group D. The high incidence of gastric cancer in Bhutan can be attributed to the high prevalence of H. pylori infection and gastric mucosal atrophy.

Determine the Prevalence of cagA and Baba of Helicobacter Pylori Isolated from Gastric Atrophic Patients

2021 ◽  
Vol 15 (6) ◽  
pp. 1620-1622
Author(s):  
Muhammad Nazir ◽  
Talal Safdar ◽  
Mushtaq Ahmad ◽  
Muhammad Ikram ◽  
Nisar Khan Sajid ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Helicobacter Pylori ◽  
Gastric Atrophy ◽  
P Value ◽  
Caga Gene ◽  
Medical Sciences ◽  
Acute Gastritis ◽  
H Pylori ◽  
Standard Techniques ◽  
Gastroduodenal Disorders

Objective: The aim of this study is to determine the prevalence of cagA and babA of helicobacter pylori isolated from gastric atrophic patients. Study Design: Descriptive/Analytical Place and Duration: The study was conducted at Medicine/Gastroenterology department of Khyber Teaching Hospital and Peshawar Institute of Medical Sciences, Peshawar for six months duration from March 2020 to August 2020. Methods: Total one hundred and twenty patients of both genders were presented in this study. Patients were aged between 20-80 years of age. Patients detailed demographics age, sex and body mass index were recorded after taking informed written consent. All patients of gastroduodenal disorders were undergone for isolation of bacteria by using standard techniques. Complete data was analyzed by SPSS 22.0 version. Results: Total 50 (41.7%) patients were males and 70 (58.3%) patients were females. Mean age of the patients were 41.96 ± 16 years with mean BMI 25.24 ± 4.8 kg/m2. Frequency of H pylori was isolated in 30 (25%) patients in which 13 patients had atrophic gastritis, 9 patients had gastric ulcer and 8 patients had acute gastritis. Prevalence of cagA gene was 16 (53.33%) and babA was 10 (33.33%) in H. pylori isolated patients. Significantly difference with p value <0.05 was observed between cagA positive strains and patients of gastric atrophic. The involvement of gastric atrophic patients was not correlated to the babA gene. Conclusion: We concluded in this study that different cagA positive H. pylori can be retrieved from gastric atrophy patients. Keywords: Gastric atrophy, Gastric cancer, cagA, babA, Helicobacter pylori

scholarly journals Prevalence, diversity and disease association of Helicobacter pylori in dyspeptic patients from Pakistan

2013 ◽  
Vol 7 (03) ◽  
pp. 220-228 ◽  
Author(s):  
Adnan Khan ◽  
Amber Farooqui ◽  
Yasir Raza ◽  
Faisal Rasheed ◽  
Hamid Manzoor ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Helicobacter Pylori ◽  
Disease Burden ◽  
Detection Rate ◽  
East Asian ◽  
Disease Association ◽  
Gastric Biopsy ◽  
Caga Gene ◽  
H Pylori ◽  
Type Strains

Introduction: The etiological association of Helicobacter pylori with gastric ulcer (GU), gastric cancer (GC), and duodenal ulcer (DU) is well-known. Understanding the epidemiology of H. pylori facilitates the estimation of disease burden in a certain population. This study presents the diversity of H. pylori genotypes and their association with different clinical outcomes among dyspeptic patients in Pakistan over a period of four years. Methodology: Gastric biopsy samples from a total of 450 dyspeptic individualswere subjected to PCR, genotypingand histology. Results: A total of 201 (45%) cases were found positive for H. pylori. The detection rate was high in GU (91%), DU (86%) and GC (83%) cases compared with those cases who had intact gastric mucosa (18%). Histology revealed the presence of infection in 68% of cases of mild/chronic nonspecific gastritis with others belonging to the GU sequel. cagA gene carriage was observed in 104 (51%) cases or mostly from DU, GU and GC groups, of which 97 were Western type strains while 3 were East-Asian type strains that are rarely observed in South Asia. vacA allelic variant s1am1 was most commonly observed, followed by s1am2, and s1bm1, with direct correlation in diseased cases (gastritis, GU, DU and GC). Prevalent genotypic combinations were s1am1/cagA- in gastritis and s1am1/cagA+ in DU, GU, and GC. Conclusions: Our study indicates the predominant circulation of Western type cagA and vacAs1am1 type H. pylori strains in Pakistan.

scholarly journals Clinicopathological Characteristics and Endoscopic Features of Early Gastric Cancers Diagnosed After Helicobacter pylori Eradication

2021 ◽  
Author(s):  
Hideki Ishibashi ◽  
Hidetoshi Takedatsu ◽  
Taro Tanabe ◽  
So Imakiire ◽  
Hiroki Matsuoka ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Helicobacter Pylori ◽  
Risk Factor ◽  
Clinicopathological Characteristics ◽  
Depressed Type ◽  
Helicobacter Pylori Eradication ◽  
Positive Group ◽  
Careful Patient ◽  
H Pylori

Abstract Background. Helicobacter pylori (H. pylori) infection is an important risk factor for developing gastric cancer. However, even after H. pylori eradication, early gastric cancer (EGC) can develop. We elucidated the characteristics of EGCs diagnosed after H. pylori eradication. Methods. Thirty-six EGCs in 32 patients diagnosed after H. pylori eradication were defined as the eradication group (H. pylori-EG). The clinicopathological and endoscopic features were compared with those of 156 EGCs in 140 patients in the H. pylori-positive group (H. pylori-PG). Twenty-nine EGC lesions in the H. pylori-EG were further divided into two subgroups: the first included six lesions of none to mild atrophic mucosa around the EGC, and the second included 23 lesions of moderate to severe atrophic mucosa around the EGC. We compared them between the two subgroups. Results. Endoscopic features of EGCs in the H. pylori-EG were characterized as small (P = 0.049) and of the depressed type (P = 0.022) compared with those in the H. pylori-PG. EGCs in the H. pylori-EG were detected on the upper region of the stomach more frequently than those in the H. pylori-PG (P = 0.002). As for submucosal ECGs in the H. pylori-EG, it was more likely to be seen in the none to mild atrophic mucosa subgroup compared to the moderate to severe atrophic gastric mucosa subgroup (P = 0.003). Conclusions. EGCs after H. pylori eradication were characterized as small and of the depressed type. Submucosal invasive EGCs developed more frequently in the none to mild atrophic mucosa after H. pylori eradication. Therefore, careful patient follow-up is important after H. pylori eradication.

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