scholarly journals Prevalence of hepatitis E virus (HEV) antibodies in Serbian blood donors

2014 ◽  
Vol 8 (10) ◽  
pp. 1322-1327 ◽  
Author(s):  
Tamaš Petrović ◽  
Diana Lupulović ◽  
Nereida Jiménez de Oya ◽  
Svetlana Vojvodić ◽  
Ana-Belén Blázquez ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Blood Donors ◽  
Hepatitis E ◽  
Enzyme Linked Immunosorbent Assay ◽  
Industrialized Countries ◽  
Limited Data ◽  
Genotype 3 ◽  
Serum Samples ◽  
High Prevalence ◽  
Polymerase Chain

Introduction: Hepatitis E virus (HEV) infection is rarely reported in industrialized countries, but recent studies have revealed quite variable seroprevalence rates among European populations, including blood donors. In Serbia, very limited data about HEV seroprevalence are available. This study aimed to determine the prevalence of anti-HEV IgG antibodies and HEV RNA in the sera of volunteer blood donors in Serbia. Methodology: Serum samples from 200 volunteer blood donors were tested for the presence of anti-HEV IgG by enzyme-linked immunosorbent assay (ELISA) using ORF-2 HEV genotype 3 recombinant proteins as antigen, and for the presence of HEV RNA by nested reverse transcriptase polymerase chain reaction (RT-PCR). Results: In total, 15% of the volunteer blood donors were seropositive. The prevalence increased with age; 21.5%, 14.2%, and 5.4% HEV seroprevalence rates were found in individuals older than 51 years, between 31 and 50 years, and in those younger than 30 years of age, respectively. However, no HEV RNA was detected in any of the individuals analyzed. Conclusions: The prevalence of anti-HEV IgG among blood donors as representatives of the general population is quite high in Serbia compared to data from many European countries. One of the reasons for this could be the high prevalence of HEV among Serbian pigs and the traditional consumption of piglet meat in the country. The relatively high HEV seroprevalence found among Serbian blood donors indicates the need for further investigation.

scholarly journals Low Hepatitis E Virus Prevalence Among Blood Donor in Dali in China

2020 ◽  
Author(s):  
Ping Fu ◽  
Baochai Lin ◽  
Bingting Wu ◽  
Ling Ke ◽  
Tianfu Yang ◽  
...  
Keyword(s):  
Blood Transfusion ◽  
Ethnic Minority ◽  
Odds Ratio ◽  
Hepatitis E Virus ◽  
Blood Donors ◽  
Hepatitis E ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Iga Antibodies ◽  
Positive Rate

Abstract BACKGROUND: Hepatitis E virus (HEV) is a nonenveloped RNA virus causing Hepatitis E worldwide. An increasing transfusion transmission cases of HEV infections from asymptomatic blood donors which causing serious illnesses in immunosuppressed recipients have been reported in the past few years. China is one of the highly prevalent regions of HEV, it is important to evaluate the risk of HEV transmission from blood transfusion. METHODS: A total of 1864 serum samples from blood donors and demographic characteristics were randomly collected from Feb to Mar 2018 in Dali city. Anti-HEV IgG, IgM and IgA antibodies and HEV antigen were examined by enzyme-linked immunosorbent assay (ELISA). HEV RNA was detected by real-time PCR. Multivariable logistic regression modelling was used to examine risk factors associated with HEV prevalence.RESULTS: Overall, the positive rate of anti-HEV IgG, IgM, and IgA antibodies was 13.36% (249/1864), 1.13%(21/1864), and 1.82%(34/1864), respectively. However, none of the 1864 serum samples was detected as HEV antigen-positive nor HEV RNA positive. The positive rate of anti-HEV IgG antibody is high as 28.57% (2/7) in the donors with isolated elevated alanine aminotransferase (ALT). Females (16.69%) had a significantly higher HEV seroprevalence than males (13.04%) (odds ratio [OR]: 1.34 [95% CI, 1.02-1.75]). Other ethnic minority (24.32%) and Bai (18.85%) donors had a significantly higher HEV seroprevalence when compared to Han (12.21%) blood donors (odds ratio [OR], 2.25 [95% CI, 1.04-4.88] for other ethnic minority, 1.65 [95% CI, 1.24-2.19] for Bai). Conclusions: Dali, Yunnan province, China is an endemic region for HEV and have a relatively low risk of HEV transmission via blood transfusion. Whether to formulate the strategy for HEV screening in blood center needed further researched.

scholarly journals Hepatitis E virus in Italy: molecular analysis of travel-related and autochthonous cases

2011 ◽  
Vol 92 (7) ◽  
pp. 1617-1626 ◽  
Author(s):  
Giuseppina La Rosa ◽  
Michele Muscillo ◽  
Valentina Spuri Vennarucci ◽  
Anna Rosa Garbuglia ◽  
Patrizia La Scala ◽  
...  
Keyword(s):  
Acute Hepatitis ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
The Body ◽  
Industrialized Countries ◽  
Genotype 3 ◽  
Serum Samples ◽  
Specific Primers ◽  
Body Of Knowledge ◽  
Acute Hepatitis E

Human hepatitis E virus (HEV) is considered an emerging pathogen in industrialized countries. The aim of the present study was to contribute to the body of knowledge available on the molecular epidemiology of acute hepatitis E in Italy. Three sets of HEV-specific primers targeting the ORF1 and ORF2 were used to examine serum samples collected from acute hepatitis patients positive for anti-HEV IgG and/or IgM, between 2007 and 2010. Seventeen patients (39.5 %) tested HEV RNA-positive: 12 infections, due to genotype 1, were associated with travel to endemic areas (Bangladesh, India and Pakistan), while five infections, due to genotype 3, were presumably autochthonous. Risk factors identified in this group included exposure to raw seafood, pork liver sausages and wild boar. Results from the present study confirm that human HEV infection in Italy is caused by different genotypes, depending on whether the infection is travel-related or autochthonous.

scholarly journals Comparison of Hepatitis E Virus Sequences from Humans and Swine, the Netherlands, 1998–2015

Viruses ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1265
Author(s):  
Boris M. Hogema ◽  
Renate W. Hakze-van der Hakze-van der Honing ◽  
Michel Molier ◽  
Hans L. Zaaijer ◽  
Wim H. M. van der van der Poel
Keyword(s):  
The Netherlands ◽  
Sequence Comparison ◽  
Hepatitis E Virus ◽  
Blood Donors ◽  
Hepatitis E ◽  
Transmission Route ◽  
Industrialized Countries ◽  
Genotype 3 ◽  
Travel History ◽  
Predominant Subtype

Pigs are suspected to be a major source of zoonotic hepatitis E virus (HEV) infection in industrialized countries, but the transmission route(s) from pigs to humans are ill-defined. Sequence comparison of HEV isolates from pigs with those from blood donors and patients in 372 samples collected in the Netherlands in 1998 and 1999 and between 2008 and 2015 showed that all sequences were genotype 3 except for six patients (with travel history). Subgenotype 3c (gt3c) was the most common subtype. While the proportion of gt3c increased significantly between 1998 and 2008, it remained constant between 2008 and 2015. Among the few circulating HEV subtypes, there was no difference observed between the human and the pig isolates. Hepatitis E viruses in humans are very likely to originate from pigs, but it is unclear why HEV gt3c has become the predominant subtype in the Netherlands.

scholarly journals Hepatitis E virus prevalence among blood donors in Dali, China

2021 ◽  
Vol 18 (1) ◽  
Author(s):  
Ping Fu ◽  
Baochai Lin ◽  
Bingting Wu ◽  
Ling Ke ◽  
Tianfu Yang ◽  
...  
Keyword(s):  
Odds Ratio ◽  
Hepatitis E Virus ◽  
Blood Donors ◽  
Rna Virus ◽  
Hepatitis E ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
The Past ◽  
Virus Prevalence ◽  
Iga Antibodies

Abstract Background Hepatitis E virus (HEV) is a nonenveloped RNA virus causing hepatitis E worldwide. The increase in transfusion-transmitted cases of HEV infections from asymptomatic blood donors causing serious illnesses among immunosuppressed recipients has been reported in the past few years. China is one of the most prevalent regions of HEV; as a result, it is important to evaluate the risk of transfusion-transmitted HEV. Methods A total of 1864 serum samples (including demographic characteristics) from blood donors were randomly collected from February to March 2018 in Dali city. Anti-HEV IgG, IgM and IgA antibodies and HEV antigen were examined by enzyme-linked immunosorbent assay (ELISA). HEV RNA was detected by real-time PCR. Multivariable logistic regression modelling was used to examine the risk factors associated with HEV prevalence. Results Overall, the positive rates of anti-HEV IgG, IgM, and IgA antibodies were 13.36% (249/1864), 1.13% (21/1864), and 1.82% (34/1864), respectively. However, none of the 1864 serum samples were HEV antigen positive or HEV RNA positive. Females (16.69%) had a significantly higher HEV seroprevalence than males (13.04%) (odds ratio [OR] 1.34 [95% CI, 1.02–1.75]). Bai (18.85%) donors had a significantly higher HEV seroprevalence than Han (12.21%) blood donors (odds ratio [OR], 1.65 [95% CI, 1.24–2.19] for Bai). Conclusions HEV showed a seroprevalence among blood donors in Yunnan Province, some of which were even recent infections, indicating a threat to the safety of blood transfusions. Whether to formulate a strategy for HEV screening in blood centres needs further research.

Seroprevalence of the hepatitis E virus antibodies among blood donors in the Qassim region, Saudi Arabia

2021 ◽  
Author(s):  
Bader Y Alhatlani ◽  
Waleed A Aljabr ◽  
Mohammed S Almarzouqi ◽  
Sami M Alhatlani ◽  
Rayan N Alzunaydi ◽  
...  
Keyword(s):  
Public Health ◽  
Saudi Arabia ◽  
Blood Transfusion ◽  
Hepatitis E Virus ◽  
Blood Donors ◽  
Indirect Elisa ◽  
Hepatitis E ◽  
Public Health Issue ◽  
Serum Samples ◽  
Major Public Health Issue

Aim: Hepatitis E virus (HEV) transmission through blood transfusion is a major public health issue worldwide. We aimed to determine the seroprevalence of HEV in blood donors in the Qassim region of Saudi Arabia. Materials & methods: Serum samples (n = 1078) were collected from volunteer blood donors and tested for the presence of anti-HEV IgG and IgM by indirect ELISA. Results: The seroprevalence of anti-HEV IgG among the blood donors was 5.7% overall. Anti-HEV IgG and IgM seropositivity were significantly higher in non-Saudi donors than in Saudi donors (22.1 vs 3 and 7.8 vs 0.2% for anti-HEV IgG and IgM, respectively). Conclusion: The seroprevalence of HEV among blood donors in the Qassim region was lower than previous estimates for other regions of the country and neighboring countries.

scholarly journals Diagnostic Performance of Five Assays for Anti-Hepatitis E Virus IgG and IgM in a Large Cohort Study

2015 ◽  
Vol 54 (3) ◽  
pp. 549-555 ◽  
Author(s):  
Heléne Norder ◽  
Marie Karlsson ◽  
Åsa Mellgren ◽  
Jan Konar ◽  
Elisabeth Sandberg ◽  
...  
Keyword(s):  
Gold Standard ◽  
Diagnostic Performance ◽  
Hepatitis E Virus ◽  
Blood Donors ◽  
Laboratory Diagnosis ◽  
Hepatitis E ◽  
Serum Samples ◽  
Large Cohort Study ◽  
No Gold Standard

Determination of anti-hepatitis E virus (anti-HEV) antibodies is still enigmatic. There is no gold standard, and results obtained with different assays often diverge. Herein, five assays were compared for detection of anti-HEV IgM and IgG. Serum samples from 500 Swedish blood donors and 316 patients, of whom 136 had suspected HEV infection, were analyzed. Concordant results for IgM and IgG with all assays were obtained only for 71% and 70% of patients with suspected hepatitis E, respectively. The range of sensitivity for anti-HEV detection was broad (42% to 96%); this was reflected in the detection limit, which varied up to 19-fold for IgM and 17-fold for IgG between assays. HEV RNA was analyzed in all patients and in those blood donors reactive for anti-HEV in any assay, and it was found in 26 individuals. Among all of the assays, both anti-HEV IgG and IgM were detected in 10 of those individuals. Twelve had only IgG and, in 7 of those 12, IgG was only detected with the two most sensitive assays. Three of the HEV-RNA-positive samples were negative for anti-HEV IgM and IgG in all assays. With the two most sensitive assays, anti-HEV IgG was identified in 16% of the blood donor samples and in 66% of patients with suspected HEV infection. Because several HEV-RNA-positive samples had only anti-HEV IgG without anti-HEV IgM or lacked anti-HEV antibodies, analysis for HEV RNA may be warranted as a complement in the laboratory diagnosis of ongoing HEV infection.

High prevalence of anti-hepatitis E virus antibodies in blood donors from South West France

2007 ◽  
Vol 80 (2) ◽  
pp. 289-293 ◽  
Author(s):  
Jean Michel Mansuy ◽  
Florence Legrand-Abravanel ◽  
Jean Pierre Calot ◽  
Jean Marie Peron ◽  
Laurent Alric ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Blood Donors ◽  
Hepatitis E ◽  
South West ◽  
High Prevalence

scholarly journals In house ELISA based on recombinant ORF2 protein underline high prevalence of IgG anti-hepatitis E virus amongst blood donors in south Brazil

PLoS ONE ◽  
2017 ◽  
Vol 12 (5) ◽  
pp. e0176409 ◽  
Author(s):  
Rafael Pandolfi ◽  
Denise Ramos de Almeida ◽  
Marcelo Alves Pinto ◽  
Luiz Carlos Kreutz ◽  
Rafael Frandoloso
Keyword(s):  
Hepatitis E Virus ◽  
Blood Donors ◽  
Hepatitis E ◽  
Recombinant Orf2 Protein ◽  
Orf2 Protein ◽  
High Prevalence ◽  
South Brazil

Prevalence of antibodies to hepatitis E virus among Japanese blood donors: Identification of three blood donors infected with a genotype 3 hepatitis E virus

2004 ◽  
Vol 73 (4) ◽  
pp. 554-561 ◽  
Author(s):  
Satoko Fukuda ◽  
Junko Sunaga ◽  
Nobuo Saito ◽  
Kuniko Fujimura ◽  
Yaeko Itoh ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Blood Donors ◽  
Hepatitis E ◽  
Genotype 3
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