scholarly journals Effect of Ibicella lutea on uropathogenic Proteus mirabilis growth, virulence, and biofilm formation

2009 ◽  
Vol 3 (10) ◽  
pp. 762-770 ◽  
Author(s):  
Vanessa Sosa ◽  
Pablo Zunino
Keyword(s):  
Proteus Mirabilis ◽  
Biofilm Formation ◽  
South American ◽  
Antimicrobial Drugs ◽  
Bacterial Growth Rate ◽  
Swarming Motility ◽  
Bacterial Morphology ◽  
Indigenous Plant ◽  
Therapeutic Problem ◽  
Tract Infections

Background: Proteus mirabilis, an important uropathogen that can cause complicated urinary tract infections (UTI), has emerged as a therapeutic problem following mutations that compromise the use of antimicrobial drugs. Due to the serious effects associated with uropathogenic P. mirabilis and the problems related to the use of antibiotics, it is necessary to develop alternative strategies for its control. The objective of this study was to assess the effect of Ibicella lutea extract, a South American indigenous plant, on growth, virulence and biofilm production of uropathogenic P. mirabilis. Methodology:  This study was based on the extract generation and the assessment of its effect on bacterial features related to virulence. These assays involved determination of antibacterial activity, swarming motility, Western blot to assess expression of fimbriae and flagella, biofilms formation, haemagglutination, haemolysis, and electron microscopy.   Results and Conclusions: I. lutea extract had an effect on bacterial growth rate and bacterial morphology. It also affected P. mirabilis swarming differentiation, hemagglutination and biofilm formation on glass and polystyrene. These findings suggest that I. lutea may have a role as an agent for the control of P. mirabilis UTI.

scholarly journals Assessment of effectiveness and safety of Ibicella lutea extract in the control of experimental Proteus mirabilis urinary tract infection

2010 ◽  
Vol 4 (12) ◽  
pp. 814-821 ◽  
Author(s):  
Vanessa Sosa ◽  
Pablo Zunino
Keyword(s):  
Urinary Tract ◽  
Proteus Mirabilis ◽  
Intestinal Microflora ◽  
Acquired Resistance ◽  
Intestinal Flora ◽  
South American ◽  
New Strategy ◽  
Indigenous Plant ◽  
Tract Infections

Introduction: Proteus mirabilis is an important cause of complicated urinary tract infections (UTI). Like many other microorganisms, P. mirabilis has acquired resistance to many antibiotics. Due to the serious effects associated with uropathogenic P. mirabilis and the problems related to the use of antibiotics, alternative strategies for its control must be developed. Previously, we studied the effect of Ibicella lutea extract, a South American indigenous plant, on in vitro uropathogenicity of P. mirabilis. We observed that I. lutea extract had an effect on various attributes associated with P. mirabilis urovirulence. The objective of this study was to assess I. lutea extract against UTI by P. mirabilis. Methodology: This study was based on the effect of I. lutea extract to prevent or treat P. mirabilis experimental UTI in mice and the influence of this administration on the normal intestinal flora. Also, we studied the toxicity, mutagenicity, and antimutagenicity of the extract. Results: In this study, while I. lutea administration showed an effect in the prevention and treatment of UTI in the mouse, the intestinal microflora did not change. The I. lutea extract was neither toxic nor mutagenic although the extract showed antimutagenic properties. Conclusion: These findings suggest that the administration of I. lutea extract could represent an interesting new strategy to control P. mirabilis UTI.

scholarly journals Antimicrobial and Antibiofilm Activities of Probiotic Lactobacilli on Antibiotic-Resistant Proteus mirabilis

2020 ◽  
Vol 8 (6) ◽  
pp. 960
Author(s):  
Mona Shaaban ◽  
Ola A. Abd El-Rahman ◽  
Bashair Al-Qaidi ◽  
Hossam M. Ashour
Keyword(s):  
Crystal Violet ◽  
Proteus Mirabilis ◽  
Biofilm Formation ◽  
Lactobacillus Casei ◽  
Lactobacillus Reuteri ◽  
Antimicrobial Activities ◽  
Antibiotic Resistant ◽  
Amoxicillin Clavulanic Acid ◽  
Probiotic Lactobacilli ◽  
Tract Infections

The emergence of biofilm-forming, multi-drug-resistant (MDR) Proteus mirabilis infections is a serious threat that necessitates non-antibiotic therapies. Antibiotic susceptibility and biofilm-forming activity of P. mirabilis isolates from urine samples were assessed by disc diffusion and crystal violet assays, respectively. Antimicrobial activities of probiotic Lactobacilli were evaluated by agar diffusion. Antibiofilm and anti-adherence activities were evaluated by crystal violet assays. While most P. mirabilis isolates were antibiotic-resistant to varying degrees, isolate P14 was MDR (resistant to ceftazidime, cefotaxime, amoxicillin-clavulanic acid, imipenem, ciprofloxacin, and amikacin) and formed strong biofilms. Cultures and cell-free supernatants of Lactobacillus casei and Lactobacillus reuteri exhibited antimicrobial and antibiofilm activities. The 1/16 concentration of untreated supernatants of L. casei and L. reuteri significantly reduced mature biofilm formation and adherence of P14 by 60% and 72%, respectively (for L. casei), and by 73% each (for L. reuteri). The 1/8 concentration of pH-adjusted supernatants of L. casei and L. reuteri significantly reduced mature biofilm formation and adherence of P14 by 39% and 75%, respectively (for L. casei), and by 73% each (for L. reuteri). Scanning electron microscopy (SEM) confirmed eradication of P14’s biofilm by L. casei. L. casei and L. reuteri could be utilized to combat Proteus-associated urinary tract infections.

scholarly journals Vitamin C in the Presence of Sub-Inhibitory Concentration of Aminoglycosides and Fluoroquinolones Alters Proteus mirabilis Biofilm Inhibitory Rate

Antibiotics ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 116 ◽  
Author(s):  
Joanna Kwiecińska-Piróg ◽  
Krzysztof Skowron ◽  
Tomasz Bogiel ◽  
Agata Białucha ◽  
Jana Przekwas ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Urinary Tract ◽  
Vitamin C ◽  
Urinary Tract Infections ◽  
Proteus Mirabilis ◽  
Biofilm Formation ◽  
Common Species ◽  
Inhibitory Effect ◽  
Acid Addition ◽  
Tract Infections

Vitamin C has antimicrobial activity and is often used as an oral supplement accompanying antibiotic treatment in urinary tract infections (UTI). Proteus mirabilis is the third common species responsible for UTIs that are mostly treated with fluoroquinolones or aminoglycosides. Treatment of the UTI caused by P. mirabilis is problematic due to the ability to form biofilm on the urinary catheters. The aim of the study was to evaluate the influence of ascorbic acid in combination with antibiotics on P. mirabilis abilities to form biofilm. The susceptibility of P. mirabilis reference strain ATCC® 29906™ and four clinical strains isolated from the urine samples of patients with urinary catheter were evaluated according to EUCAST recommendations. The influence of ascorbic acid (0.4 mg × mL−1) in combination with antibiotics on biofilm formation was evaluated spectrophotometrically. Aminoglycosides at sub-inhibitory concentrations more successfully limited biofilm formation by P. mirabilis strains without ascorbic acid addition. Inhibition rate differences at the lowest concentrations of gentamicin and amikacin were statistically significant (p ≤ 0.05). Ascorbic acid addition to the culture medium limited the inhibitory effect of fluoroquinolones, facilitating biofilm formation by P. mirabilis strains. The addition of ascorbic acid during aminoglycosides therapy may disturb treatment of urinary tract infections related to the presence of P. mirabilis biofilm.

Chlorpromazine-impregnated catheters as a potential strategy to control biofilm-associated urinary tract infections

2019 ◽  
Vol 14 (12) ◽  
pp. 1023-1034 ◽  
Author(s):  
José JC Sidrim ◽  
Bruno R Amando ◽  
Francisco IF Gomes ◽  
Marilia SMG do Amaral ◽  
Paulo CP de Sousa ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract ◽  
Klebsiella Pneumoniae ◽  
Urinary Tract Infections ◽  
Proteus Mirabilis ◽  
Biofilm Formation ◽  
Antibiofilm Activity ◽  
Potential Strategy ◽  
Tract Infections

Aim: This study proposes the impregnation of Foley catheters with chlorpromazine (CPZ) to control biofilm formation by Escherichia coli, Proteus mirabilis and Klebsiella pneumoniae. Materials & methods: The minimum inhibitory concentrations (MICs) for CPZ and the effect of CPZ on biofilm formation were assessed. Afterward, biofilm formation and the effect of ciprofloxacin and meropenem (at MIC) on mature biofilms grown on CPZ-impregnated catheters were evaluated. Results: CPZ MIC range was 39.06–625 mg/l. CPZ significantly reduced (p < 0.05) biofilm formation in vitro and on impregnated catheters. In addition, CPZ-impregnation potentiated the antibiofilm activity of ciprofloxacin and meropenem. Conclusion: These findings bring perspectives for the use of CPZ as an adjuvant for preventing and treating catheter-associated urinary tract infections.

scholarly journals Pathogenesis of Proteus mirabilis in Catheter-Associated Urinary Tract Infections

2021 ◽  
pp. 1-8
Author(s):  
Fei Yuan ◽  
Ziye Huang ◽  
Tongxin Yang ◽  
Guang Wang ◽  
Pei Li ◽  
...  
Keyword(s):  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Proteus Mirabilis ◽  
Immune Escape ◽  
Endotoxic Shock ◽  
Urine Retention ◽  
Indwelling Catheter ◽  
Swarming Motility ◽  
Historical Perspectives ◽  
Tract Infections

Proteus mirabilis (PM) is a Gram-negative rod-shaped bacterium and widely exists in the natural environment, and it is most noted for its swarming motility and urease activity. PM is the main pathogen causing complicated urinary tract infections (UTIs), especially catheter-associated urinary tract infections. Clinically, PM can form a crystalline biofilm on the outer surface and inner cavity of the urethral indwelling catheter owing to its ureolytic biomineralization. This leads to catheter encrustation and blockage and, in most cases, is accompanied by urine retention and ascending UTI, causing cystitis, pyelonephritis, and the development of bladder or kidney stones, or even fatal complications such as septicemia and endotoxic shock. In this review, we discuss how PM is mediated by a catheter into the urethra, bladder, and then rose to the kidney causing UTI and the main virulence factors associated with different stages of infection, including flagella, pili or adhesins, urease, hemolysin, metal intake, and immune escape, encompassing both historical perspectives and current advances.

scholarly journals Mannose-Resistant Proteus-Like Fimbriae Are Produced by Most Proteus mirabilis Strains Infecting the Urinary Tract, Dictate the In Vivo Localization of Bacteria, and Contribute to Biofilm Formation

2004 ◽  
Vol 72 (12) ◽  
pp. 7294-7305 ◽  
Author(s):  
Angela M. Jansen ◽  
Virginia Lockatell ◽  
David E. Johnson ◽  
Harry L. T. Mobley
Keyword(s):  
Urinary Tract ◽  
Proteus Mirabilis ◽  
Biofilm Formation ◽  
Constitutive Expression ◽  
Etiologic Agent ◽  
Phase Variable ◽  
Wild Type ◽  
Bladder Tissue ◽  
Tract Infections

ABSTRACT Proteus mirabilis, an etiologic agent of complicated urinary tract infections, expresses mannose-resistant Proteus-like (MR/P) fimbriae whose expression is phase variable. Here we examine the role of these fimbriae in biofilm formation and colonization of the urinary tract. The majority of wild-type P. mirabilis cells in transurethrally infected mice produced MR/P fimbriae. Mutants that were phase-locked for either constitutive expression (MR/P ON) or the inability to express MR/P fimbriae (MR/P OFF) were phenotypically distinct and swarmed at different rates. The number of P. mirabilis cells adhering to bladder tissue did not appear to be affected by MR/P fimbriation. However, the pattern of adherence to the bladder surface was strikingly different. MR/P OFF colonized the lamina propria underlying exfoliated uroepithelium, while MR/P ON colonized the luminal surfaces of bladder umbrella cells and not the exfoliated regions. Wild-type P. mirabilis was usually found colonizing intact uroepithelium, but it occasionally adhered to exfoliated areas. MR/P ON formed significantly more biofilm than either P. mirabilis HI4320 (P = 0.03) or MR/P OFF (P = 0.05). MR/P OFF was able to form a biofilm similar to that of the wild type. MR/P ON formed a three-dimensional biofilm structure as early as 18 h after the initiation of the biofilm, while MR/P OFF and the wild type did not. After 7 days, however, P. mirabilis HI4320 formed a 65-μm-thick biofilm, while the thickest MR/P ON and MR/P OFF biofilms were only 12 μm thick. We concluded that MR/P fimbriae are expressed by most P. mirabilis cells infecting the urinary tract, dictate the localization of bacteria in the bladder, and contribute to biofilm formation.

scholarly journals The high-affinity phosphate transporter Pst in Proteus mirabilis HI4320 and its importance in biofilm formation

Microbiology ◽  
2009 ◽  
Vol 155 (5) ◽  
pp. 1523-1535 ◽  
Author(s):  
G. A. O'May ◽  
S. M. Jacobsen ◽  
M. Longwell ◽  
P. Stoodley ◽  
H. L. T. Mobley ◽  
...  
Keyword(s):  
Human Urine ◽  
Proteus Mirabilis ◽  
Biofilm Formation ◽  
Wild Type ◽  
Transposon Mutants ◽  
Tract Infections ◽  
Micro Organisms ◽  
Pst System ◽  
Specific Transport System

Proteus mirabilis causes urinary tract infections (UTIs) in individuals requiring long-term indwelling catheterization. The pathogenesis of this uropathogen is mediated by a number of virulence factors and the formation of crystalline biofilms. In addition, micro-organisms have evolved complex systems for the acquisition of nutrients, including the phosphate-specific transport system, which has been shown to be important in biofilm formation and pathogenesis. A functional Pst system is important during UTIs caused by P. mirabilis HI4320, since transposon mutants in the PstS periplasmic binding protein and the PstA permease protein were attenuated in the CBA mouse model of UTI. These mutants displayed a defect in biofilm formation when grown in human urine. This study focuses on a comparison of the proteomes during biofilm and planktonic growth in phosphate-rich medium and human urine, and microscopic investigations of biofilms formed by the pst mutants. Our data suggest that (i) the Δpst mutants, and particularly the ΔpstS mutant, are defective in biofilm formation, and (ii) the proteomes of these mutants differ significantly from that of the wild-type. Therefore, since the Pst system of P. mirabilis HI4320 negatively regulates biofilm formation, this system is important for the pathogenesis of these organisms during complicated UTIs.

scholarly journals Pathogenic Factors Correlate With Antimicrobial Resistance Among Clinical Proteus mirabilis Strains

2020 ◽  
Vol 11 ◽  
Author(s):  
Aneta Filipiak ◽  
Magdalena Chrapek ◽  
Elżbieta Literacka ◽  
Monika Wawszczak ◽  
Stanisław Głuszek ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Proteus Mirabilis ◽  
Biofilm Formation ◽  
Virulence Gene ◽  
Multidrug Resistant ◽  
Swarming Motility ◽  
Gene Profiles ◽  
Pathogenic Factors ◽  
Antibiotic Susceptibility Patterns ◽  
Susceptibility Patterns

Proteus mirabilis is the third most common etiological factor of urinary tract infection. It produces urease, which contributes to the formation of a crystalline biofilm, considered to be one of the most important virulence factors of P. mirabilis strains, along with their ability to swarm on a solid surface. The aim of this study was to analyze the pathogenic properties of two selected groups of clinical P. mirabilis isolates, antimicrobial susceptible and multidrug resistant (MDR), collected from hospitals in different regions in Poland. The strains were examined based on virulence gene profiles, urease and hemolysin production, biofilm formation, and swarming properties. Additionally, the strains were characterized based on the Dienes test and antibiotic susceptibility patterns. It turned out that the MDR strains exhibited kinship more often than the susceptible ones. The strains which were able to form a stronger biofilm had broader antimicrobial resistance profiles. It was also found that the strongest swarming motility correlated with susceptibility to most antibiotics. The correlations described in this work encourage further investigation of the mechanisms of pathogenicity of P. mirabilis.

scholarly journals Transcriptional Analysis of the MrpJ Network: Modulation of Diverse Virulence-Associated Genes and Direct Regulation ofmrpFimbrial andflhDCFlagellar Operons in Proteus mirabilis

2015 ◽  
Vol 83 (6) ◽  
pp. 2542-2556 ◽  
Author(s):  
Nadine J. Bode ◽  
Irina Debnath ◽  
Lisa Kuan ◽  
Anjelique Schulfer ◽  
Maureen Ty ◽  
...  
Keyword(s):  
Proteus Mirabilis ◽  
Transcriptional Analysis ◽  
Gene Promoters ◽  
Swarming Motility ◽  
Content Type ◽  
Cellular Processes ◽  
Type Vi Secretion ◽  
Strict Regulation ◽  
Tract Infections

The enteric bacteriumProteus mirabilisis associated with a significant number of catheter-associated urinary tract infections (UTIs). Strict regulation of the antagonistic processes of adhesion and motility, mediated by fimbriae and flagella, respectively, is essential for disease progression. Previously, the transcriptional regulator MrpJ, which is encoded by themrpfimbrial operon, has been shown to repress both swimming and swarming motility. Here we show that MrpJ affects an array of cellular processes beyond adherence and motility. Microarray analysis found that expression ofmrpJmimicking levels observed during UTIs leads to differential expression of 217 genes related to, among other functions, bacterial virulence, type VI secretion, and metabolism. We probed the molecular mechanism of transcriptional regulation by MrpJ using transcriptional reporters and chromatin immunoprecipitation (ChIP). Binding of MrpJ to two virulence-associated target gene promoters, the promoters of the flagellar master regulatorflhDCandmrpitself, appears to be affected by the condensation state of the native chromosome, although both targets share a direct MrpJ binding site proximal to the transcriptional start. Furthermore, anmrpJdeletion mutant colonized the bladders of mice at significantly lower levels in a transurethral model of infection. Additionally, we observed thatmrpJis widely conserved in a collection of recent clinical isolates. Altogether, these findings support a role of MrpJ as a global regulator ofP. mirabilisvirulence.

scholarly journals Virulence genes and antimicrobial resistance pattern in Proteus mirabilis strains isolated from patients attended with urinary infections to Tertiary Hospitals, in Iran

2021 ◽  
Vol 21 (4) ◽  
pp. 1677-84
Author(s):  
Azardokht Tabatabaei ◽  
Khadijeh Ahmadi ◽  
Alireza Namazi Shabestari ◽  
Nastaran Khosravi ◽  
Ali Badamchi
Keyword(s):  
Antibiotic Resistance ◽  
Antimicrobial Resistance ◽  
Virulence Factors ◽  
Proteus Mirabilis ◽  
Biofilm Formation ◽  
Virulence Genes ◽  
Standard Procedure ◽  
Tertiary Hospital ◽  
Resistance Pattern ◽  
Tract Infections

Background: Proteus mirabilis is a frequent reason for catheter-associated urinary tract infections (UTIs). The aim of this study was to identify virulence genes and antimicrobial resistance patterns in P. mirabilis strains isolated from patients whoattended a tertiary hospital in Iran. Methods: In this study, 100 P. mirabilis strains from urine samples were isolated. These isolated strains were identified by biochemical and PCR-based tests, and their antibiotic resistance was profiled through a standard procedure using 14 antibiotics.PCR assays were used to detect virulence-related genes in P. mirabilis strains. The biofilm formation of each P. mirabilis strain was examined. Results: Of the 100 P. mirabilis isolates, 16 (16%) were multidrug-resistant. High resistance was observed against cotrimoxazole (97%), nalidixic acid (93%), cefotaxime (77%), and amoxicillin (62%). Sixty of the 100 isolates showed resistance against extended-spectrum cephalosporins. The prevalence rates of the genes related to the virulence factors in this study were mrpH (100%), ucaA (91%), hpmA (94%), zapA (95%), ptaA (100%), ureG (100%), pmfA (100%), fliC (97%), and mrpA (90%) using PCR method. Strong biofilm formation was observed in 20% (5/25) of the strains isolated fromnon-catheterized samples and 80% (20/25) of strains isolated from catheterized samples. Conclusions: Resistance to antibiotics and the prevalence of pathogenicity genes are high in Proteus mirabilis strains iolated from UTIs. Keywords: Antibiotic resistance; Proteus mirabilis; biofilm; virulence factors.

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