scholarly journals Antimicrobial susceptibilities of enteric bacterial pathogens isolated in Kathmandu, Nepal, during 2002-2004

2011 ◽  
Vol 5 (03) ◽  
pp. 163-168 ◽  
Author(s):  
Palpasa Kansakar ◽  
Pankaj Baral ◽  
Sarala Malla ◽  
Gokarna Raj Ghimire
Keyword(s):  
Vibrio Cholerae ◽  
Shigella Dysenteriae ◽  
Salmonella Spp ◽  
Shigella Species ◽  
National Public Health ◽  
Shigella Spp ◽  
Stool Samples ◽  
Culture Positive ◽  
Vibrio Spp

Introduction: The prevalence and antimicrobial susceptibility patterns of the bacterial enteropathogens Vibrio cholerae, Salmonella species and Shigella species were investigated. Methodology: A total of 877 stool samples were received for culture at the National Public Health Laboratory (NPHL), Kathmandu, Nepal, during January 2002 to December 2004, from diarrhoea patients attending Shukraraj Tropical Infectious Hospital and referral outpatients. All samples collected were processed for isolation and antibiotic susceptibility testing of Vibrio cholerae, Salmonella spp. and Shigella spp. Results: Of the 877 stool samples, 148 (16.8%) were culture positive for one of the three bacterial enteropathogens investigated. Among them, Vibrio cholerae, Shigella spp. and Salmonella spp. accounted for 98/877 (11.1%), 41/877 (4.6%), 9/877 (1.02%) of the isolates respectively. A year-to-year variation was seen in the type of predominant organism, with Shigella spp. being the most prevalent in 2002 and 2003 and Vibrio spp. in 2004. In all three years, Vibrio cholerae were encountered only during the months of April to June while Salmonella spp. and Shigella spp. were isolated throughout the whole year. All Vibrio cholerae and Salmonella isolates were susceptible to ciprofloxacin.  All Shigella isolates were susceptible to ceftriaxone. Ciprofloxacin resistance was observed among isolates of Shigella dysenteriae type-1 isolated after 2003. Conclusion: Vibrio cholerae, Salmonella and Shigella infections are prevalent in Kathmandu, Nepal. A gradual increase in resistance to commonly used antimicrobials was seen among bacterial enteropathogens. Antimicrobial resistance surveillance is necessary to guide empirical treatment.

scholarly journals SEROTYPING AND ANTIBIOTIC SENSITIVITY TESTING OF ENTEROBACTERIAL PATHOGENS IN DIARRHOEAL PATIENTS OF KATHMANDU

2003 ◽  
Vol 41 (143) ◽  
pp. 392-6
Author(s):  
Abhignya Subedi ◽  
C D Shrestha ◽  
R P Adhikari
Keyword(s):  
Public Health ◽  
Shigella Flexneri ◽  
Antibiotic Sensitivity ◽  
Shigella Dysenteriae ◽  
Salmonella Spp ◽  
Sensitivity Testing ◽  
E Coli ◽  
The Family ◽  
National Public Health ◽  
Shigella Spp

In a study carried out at National Public Health Laboratory, Teku, 236 samples werecollected from the diarrhoeal patients and half of them were The patients below 15years of age. Altogether 61 pathogens belonging to the family Enterobacteriaceaewere isolated. The presence of pathogens in the patterns below 5 years was highlysignificant (P<0.05%). E coli was most common (68.8%) followed by Salmonella spp.(16.4%) and Shigella spp. (14.7%). Enteropathogenic was E. coli was the most frequentisolate (71.4%) among the pathogenic E. coli. Similarly, Salmonella typhimuriumwas the commonest salmonellae and Shigella dysenteriae and Shigella flexneri werethe most commonly isolated shigellae. Most of the pathogens were sensitive toCiprofloxacin (72.1%) and least was sensitive to Ampicillin (9.8%). Only 4.9% of thepathogens were sensitive to all the antibiotics tested.

scholarly journals High Resistance of Salmonella spp. and Shigella spp. in Blood and Stool Cultures from the Sukraraj Tropical and Infectious Disease Hospital, Kathmandu, Nepal, 2015−2019

2021 ◽  
Vol 6 (2) ◽  
pp. 59
Author(s):  
Anup Bastola ◽  
Prajjwal Pyakurel ◽  
Rajan Bikram Rayamajhi ◽  
Saugat Shrestha ◽  
Pruthu Thekkur ◽  
...  
Keyword(s):  
Infectious Disease ◽  
Antibiotic Resistance ◽  
Bacterial Isolates ◽  
Salmonella Spp ◽  
Infectious Disease Hospital ◽  
Shigella Spp ◽  
Stool Samples ◽  
Disease Hospital ◽  
Stool Cultures ◽  
Culture Positive

Antimicrobial resistance (AMR) is an increasing global concern, particularly in Southeast Asian countries like Nepal. The aim of this study was to determine the proportion of Salmonella spp. and Shigella spp. among culture-positive bacterial isolates in blood and stool samples from 2015 to 2019 and their AMR pattern. Routinely collected data were abstracted from medical records and laboratory electronic databases of the Sukraraj Tropical and Infectious Disease Hospital (STIDH), Kathmandu, Nepal. All culture-positive bacterial isolates from blood and stool samples were included in the study. Among 390 blood cultures positive for bacterial isolates, Salmonella spp. were isolated in 44%, with S. Typhi being the most frequent (34%). Antibiotic resistance was demonstrated among Salmonella spp. to ciprofloxacin (68%), ofloxacin (16%), amoxicillin (13%) and cotrimoxazole (5%). Of the 357 stool cultures positive for bacterial isolates, the proportion of Shigella spp. isolated was 31%. Antibiotic resistance among Shigella spp. was demonstrated to cotrimoxazole (59%), tetracycline (40%), amoxicillin (38%) and ciprofloxacin (25%). Salmonella spp. and Shigella spp. were the most predominant organisms among all the bacterial isolates in blood and stool cultures, respectively. Nalidixic acid was the antibiotic to which both Salmonella spp. and Shigella spp. were most resistant.

scholarly journals Quality assessment of Zeus faber (Peter’s fish) ovaries regularly commercialized for human consumption

2018 ◽  
Vol 7 (1) ◽  
Author(s):  
Filippo Giarratana ◽  
Graziella Ziino ◽  
Valerio D'Andrea ◽  
Antonio Panebianco ◽  
Alessandro Giuffrida
Keyword(s):  
Quality Assessment ◽  
Mean Value ◽  
Raw Material ◽  
Human Consumption ◽  
Salmonella Spp ◽  
The Mean ◽  
Fresh Products ◽  
Vibrio Spp ◽  
Zeus Faber

n the last few years, the consumption of fish eggs has increased rapidly, finding widespread use also in mass catering. This increase has involved also those of the Peter’s fish (Zeus faber). Females of this species, by their reproductive characteristics, have highly developed gonads in different periods of the year, making the raw material easy to find. The aim of the present study was to perform a quality assessment of Zeus faber ovaries regularly commercialized for human consumption. A total number of 34 samples, divided in fresh (11) and frozen (23), were processed for microbiological characterization, parasitological and histological evaluations. Fresh and frozen samples have significant (P<0.01) differences in total bacterial charge, with values of 4.75±0.5 Log CFU/g and 3.65±0.7 Log CFU/g respectively. The mean value of Enterobacteriaceae was 2.58±0.7 Log CFU/g in fresh products, while 52.17% (12) of frozen samples reported loads of <1 Log CFU/g. No Salmonella spp. and Listeria monocytogenes were found. Aeromonas spp. was detected in two frozen sample (with loads of 2.2 and <1 Log CFU/g) and in 5 fresh ovaries with value ranged from 1.70 to 3.48 Log CFU/g. Vibrio spp. was found in 4 (36.36%) and 3 (13.04%) of fresh and frozen samples respectively, with loads always <1 Log CFU/g. All 31 Vibrio strains isolated, were identified as Vibrio alginolyticus, and 61.29% (19) of them was positive for the ToxRS factor and 6.45% (2) for ToxR. The 47.06% (16) of total samples showed infestations by larvae of Anisakis Type 1 in the serous and inside the ovary. In this last case, histologically it was found to be free larvae. This study attested satisfactory hygiene conditions for Zeus faber ovaries currently marked for human consumption. The presence of potentially pathogenic strains of V. alginolyticus and Aeromonas spp., but above all the frequent infestation by Anisakis larvae, represent a potentially hazard for the consumer.

scholarly journals Envolvimento de Aeromonas em surto de doença diarréica aguda em São Bento do Una, Pernambuco

2006 ◽  
Vol 39 (2) ◽  
pp. 217-220 ◽  
Author(s):  
Ernesto Hofer ◽  
Cristhiane Moura Falavina dos Reis ◽  
Grace Nazareth Diogo Theophilo ◽  
Valdelúcia Oliveira Cavalcanti ◽  
Nancy Veloso de Lima ◽  
...  
Keyword(s):  
Vibrio Cholerae ◽  
Salmonella Spp ◽  
Aeromonas Veronii ◽  
Aeromonas Caviae ◽  
Shigella Spp

No primeiro semestre de 2004, ocorreu um surto de diarréia em São Bento do Una, Pernambuco, registrando-se 2.170 casos. Nas 582 coproculturas realizadas, 145 (25%) revelaram um enteropatógeno bacteriano, destacando 114 casos (19,5%) com a participação de Aeromonas, representadas por Aeromonas caviae (57/9,8%), Aeromonas veronii biovar sobria (23/3,9%), Aeromonas veronii biovar veronii (15/2,6%) e outras espécies (19/3,2%). Nos 31 episódios restantes (5,3%), foram detectados: V. cholerae O1 Ogawa toxigênico (18/3,1%), Salmonella spp (8/1,4%), Shigella spp (3/0,5%) e Vibrio cholerae não O1/não O139 (2/0,3%).

Molecular Strategies for the Detection, Identification, and Differentiation between Enteroinvasive Escherichia coli and Shigella spp.

2005 ◽  
Vol 68 (2) ◽  
pp. 239-245 ◽  
Author(s):  
CESAR I. BIN KINGOMBE ◽  
MARIA-LUCIA CERQUEIRA-CAMPOS ◽  
JEFFREY M. FARBER
Keyword(s):  
Escherichia Coli ◽  
Multiplex Pcr ◽  
Shigella Flexneri ◽  
Shigella Dysenteriae ◽  
Shigella Boydii ◽  
Epidemiological Tool ◽  
Pcr Rflp ◽  
Shigella Spp

A strategy for the detection, identification, and differentiation of enteroinvasive Escherichia coli (EIEC) and Shigella spp. has been developed. The strategy includes (i) a multiplex PCR for the amplification of two virulence genes, i.e., iuc (222 bp) and ipaH (629 bp); (ii) amplification of the ial gene (a 1,038-bp amplicon) located within a large plasmid; and (iii) restriction fragment length polymorphism (RFLP) of the ial gene amplicon. The multiplex PCR provided three patterns. Pattern 1 (iuc−/ipaH+) was found in 10 (67%) of 15 EIEC strains tested, pattern 2 (iuc+/ipaH−) in only 2 (4.4%) of 46 non-EIEC isolates, whereas pattern 3 (iuc+/ipaH+) was observed in all Shigella spp. and also in 5 (33%) of 15 EIEC strains tested. The pattern 3 EIEC strains were all positive for the ial gene. The PCR-RFLP of the ial gene amplicon using the endonuclease AclI was used to differentiate Shigella spp. from the EIEC strains that belonged to pattern 3. The ial gene was present in 21 (38%) of 56 and 6 (40%) of 15 Shigella spp. and EIEC strains tested, respectively. The PCR-RFLP of the ial gene amplicon divided the strains in two types. Type 1 did not contain the restriction enzyme site and was found in 6 (100%) of 6 EIEC strains, 4 (80%) of 5 Shigella boydii, and 4 (100%) of 4 Shigella dysenteriae strains tested. Type 2, which gave two fragments of 286 and 752 bp, was observed in 5 (83%) of 6 Shigella flexneri strains and 6 (100%) of 6 Shigella sonnei strains. Detection, identification, and differentiation of Shigella spp. and EIEC were achieved by analyses of the PCR patterns and RFLP types. To our knowledge, this is the first study to demonstrate a simple and rapid method for detecting, identifying, and differentiating, at the molecular level, Shigella spp. and EIEC strains. This method will have tremendous utility as an epidemiological tool and in helping to develop policies, risk assessments, and national and international methods for Shigella spp.

scholarly journals Assessment of Antibiotics and Fluoride Toothpastes against Pathogenic Bacteria associated with Dental Caries

2018 ◽  
pp. 1-10
Keyword(s):  
Dental Caries ◽  
Pathogenic Bacteria ◽  
Diffusion Method ◽  
Significant Activity ◽  
Salmonella Spp ◽  
E Coli ◽  
Shigella Spp ◽  
Test Organisms ◽  
Susceptibility Tests ◽  
Vibrio Spp

Dental caries is the most contagious disease throughout the world. In the present study, a total of 100 dental caries patients were selected and samples from these patients were collected through sterile pincers for microbiological examination. A total of 9 different pathogenic bacterial isolates were identified through conventional culturing technique and among them maximum number of occurrence was by Salmonella spp (26.13%), followed by E. coli (17.39%), Proteus spp (17.39%), Shigella spp (8.69%), Pseudomonas spp (8.69%), Vibrio spp (8.69%), Enterobacter spp (4.34%), Yersinia spp (4.34%) and S. mutans (4.34%). Furthermore, antibiotic susceptibility tests were performed by 9 different commercially available antibiotics i.e. vancomycin, erythromycin, clindamycin, amoxicillin, tetracycline, rifampicin, ampicillin, ticarcillin and metronidazole/silver sulphadizine. Kirby Bauer disc diffusion method was used against test organisms and it was observed that all these antibiotics expressed significant activity against greater part of test organisms but few of them showed resistance to metronidazole/silver sulphadizine. In addition to this, different toothpastes encoded as TP-1, TP-2, TP-3, TP-4 and TP-5 were used at a concentration of 20 mg/mL in order to evaluate their activity against test organisms and it was observed that all these toothpastes showed significant activity against isolated bacteria. It was concluded that all these toothpastes contained fluoride which supports to avoid tooth decay and promote minty fresh breath. Furthermore, it was recommended that avoid usage of too much sweets, chocolates, candies especially in children/adults, and there must be a proper use of toothpastes twice a day at every stage of life. Keywords: Dental Caries, Pathogenic Bacteria, Antibiotics, Fluoride Toothpastes

scholarly journals Factors association with some bacteria cause diarrhea disease among children under 5 years old

2018 ◽  
Vol 28 (3) ◽  
pp. 48
Author(s):  
Khadija Al-Mizury
Keyword(s):  
Bacterial Infection ◽  
Diarrheal Disease ◽  
Culture Media ◽  
Tap Water ◽  
Personal Hygiene ◽  
Salmonella Spp ◽  
Shigella Spp ◽  
Stool Samples ◽  
Diarrhea Disease ◽  
Children Under 5

Aim: “Diarrhea has been a common cause of morbidity and mortality in children under 5 years old. This study was intended to assessment level of personal hygiene, type of water taken by children, crowding index and some factors associated with some bacterial infection in children under 5 years.”Material and Methods: This study enrolled 143 children under 5 years with clinical evidence of diarrheal disease through the period extending from 15/4/2016 to 30/8/2016, who were admitted to Baghdad teaching hospital,   stool samples were collected from children who had diarrhea were inoculated on selective culture media using standard method. “The isolate were identified depending on morphological feature of colonies and from all media biochemically using API 20E system.”Results: A total bacterial infection was observed (13.9%). Specific prevalence of species bacteria is as follows,” E.coli (7.7%), and Shigella spp. (2%), and Salmonella spp. (3.5%) and V. cholera (0.7%).” Finding from our study indicate that patient in the age group >5 years of age were more likely to have diarrhea than those who were younger, and Children that consumed tap water were more infected with bacteria (9.7%). In this study, crowding index were associated with diarrheal disease,” children from households with 1 or 2 people per room were (1.4%) less likely to have diarrhea compared to children from household with more than 3 people per room (30%). Our results indicate that availability of house hold sanitation facilities, access to filter and clean water, good personal hygiene and butter nutrition were all associated with lower incidence of diarrhea.” 

scholarly journals Detection of Vibrio spp., Salmonella spp., and Shigella spp. among the frozen food samples employing enrichment culture technique

2016 ◽  
Vol 5 (1) ◽  
pp. 26-29 ◽  
Author(s):  
Tahmina Shammi
Keyword(s):  
Enrichment Culture ◽  
Bacterial Load ◽  
False Negative ◽  
Food Samples ◽  
Food Preservation ◽  
Salmonella Spp ◽  
Potential Health ◽  
Frozen Food ◽  
Shigella Spp ◽  
Vibrio Spp

Freezing has long been an established method for food preservation. Freezing temperature may act as a stress factor for microbial cells, transforming the cells into injured or dormant state. Upon inoculation, these debilitated cells cannot grow on solid media and hence produce false negative results. Foods contaminated with injured cells of pathogenic bacterial strains are of potential health risk. Employing enrichment cultivation technique, present study attempted to detect such injured, dormant or viable but non culturable (VBNC) cells in different frozen food samples, collected from local markets and super-shops of Dhaka metropolis. Compared to the conventional cultivation means, the enrichment procedure revealed a significant increase in bacterial burden as well as increase in the pathogenic load. A maximum of 3 log increase in case of total bacterial load while 4 log, 5 log and 2 log increase in case of Vibrio spp., Salmonella spp. and Shigella spp., consecutively were observed. These findings clearly demonstrated the presence of injured cells in frozen foods which could be lethal under normal condition thereby posing public health risk.Stamford Journal of Microbiology, Vol.5(1) 2015: 26-29

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