scholarly journals Distribution and antibiotic susceptibility profiles of Aeromonas spp. from different aquatic environments in north Lebanon

2018 ◽  
Vol 12 (02.1) ◽  
pp. 6S
Author(s):  
Rayane Rafei ◽  
Imad Al Kassaa ◽  
Marwan Osman ◽  
Fouad Dabboussi ◽  
Monzer Hamze
Keyword(s):  
Multiplex Pcr ◽  
Water Samples ◽  
Antibiotic Susceptibility ◽  
Aquatic Environments ◽  
Beta Lactams ◽  
Culture Methods ◽  
Resistant Strains ◽  
Seawater Samples ◽  
Susceptibility Profiles ◽  
Different Sources

Introduction: Bacteria of Aeromonas genus are ubiquitous organisms in aquatic environments. This work aims to explore the presence of four clinically relevant species in Lebanese waters and to study their susceptibility to antibiotics. Methods: One hundred water samples are collected in northern Lebanon from different sources (river, springs, wells, ponds, sea, chlorinated water and sewage) and analyzed by culture methods. The isolates were identified at genus level by a PCR assay targeting gcat gene. The presence of four species (A. hydrophila, A. caviae, A. media and A. veronii) was investigated by a multiplex PCR. The antibiotic susceptibility was also studied. Results: Aeromonas spp. was detected in 28% of investigated samples. In total, 38 isolates were identified as Aeromonas spp (10 out of 7 river water samples, 9 out of 35 spring, 9 among 26 wells, 5 out of 4 sewage samples, 2 out of 5 ponds, 2 out of 8 seawater samples and 1 out of 15 chlorinated water samples). Aeromonas hydrophila was the predominant species (19 isolates; 50%). The 3 other species were found to a lesser extent: A. caviae (3 isolates; 7.9%), A. veronii (2 isolates; 5.3%) and A. media (2 isolates; 5.3%). 12 other isolates (31.5%) remained unidentified by the used multiplex PCR technique. All isolates were resistant to more than one antibiotic. The most common resistance concerned beta-lactams. Conclusion: This study has highlighted the interesting distribution of these species in aquatic biotopes in Lebanon and the threat of potential transmission of these resistant strains to humans.

Direct Detection and Identification of Arcobacter Species by Multiplex PCR in Chicken and Wastewater Samples from Spain

2007 ◽  
Vol 70 (2) ◽  
pp. 341-347 ◽  
Author(s):  
A. GONZÁLEZ ◽  
S. BOTELLA ◽  
R. M. MONTES ◽  
Y. MORENO ◽  
M. A. FERRÚS
Keyword(s):  
Multiplex Pcr ◽  
Water Samples ◽  
Direct Detection ◽  
Pcr Amplification ◽  
Pcr Assay ◽  
Direct Pcr ◽  
Culture Methods ◽  
Multiplex Pcr Assay ◽  
Detection And Identification ◽  
Wastewater Samples

Twenty-two chicken livers, 10 chicken carcasses, and 15 wastewater samples were processed and analyzed for Arcobacter by PCR and traditional culture methods. Samples were enriched for 24 and 48 h, incubated at 30°C under aerobic conditions, and streaked on blood selective media. To determine the best isolation conditions, 20 samples also were processed under microaerophilic conditions at 37°C. Simple and multiplex PCR assays were used directly with enrichment broths and isolated strains. Seventeen Arcobacter strains were isolated from chicken samples, and A. butzleri was the only Arcobacter species identified. The direct PCR assay revealed that 29 of the 32 chicken samples were contaminated with Arcobacter. A. butzleri was the most frequently detected species, although Arcobacter cryaerophilus also was present in some of the samples and Arcobacter skirrowii occasionally was detected. All the wastewater samples were positive by PCR assay for Arcobacter after 24 h of enrichment. A. butzleri and A. cryaerophilus were detected with the multiplex PCR assay. Fourteen Arcobacter strains were isolated from 10 of the 15 water samples analyzed; 7 were identified as A. butzleri and the remaining 7 were A. cryaerophilus. Both for chicken and water samples, Arcobacter detection rate for PCR amplification was higher than for culture isolation. These results indicate the high prevalence of Arcobacter in chicken and wastewater and the inadequacy of available cultural methods for its detection. The species-specific multiplex PCR assay is a rapid method for assessing Arcobacter contamination in chicken and wastewater samples and is a viable alternative to biochemical identification of isolated strains.

scholarly journals Characterization and Phylogenetic Analysis of Campylobacter Species Isolated from Paediatric Stool and Water Samples in the Northwest Province, South Africa

2019 ◽  
Vol 16 (12) ◽  
pp. 2205 ◽  
Author(s):  
Chukwu ◽  
Luther King Abia ◽  
Ubomba-Jaswa ◽  
Obi ◽  
Dewar
Keyword(s):  
South Africa ◽  
Water Samples ◽  
Disease Transmission ◽  
Antibiotic Susceptibility ◽  
Genetic Relatedness ◽  
Diffusion Method ◽  
23S Rrna ◽  
Antibiotic Resistant ◽  
Susceptibility Profiles ◽  
Northwest Province

Antibiotic-resistant Campylobacter could adversely affect treatment outcomes, especially in children. We investigated the antibiotic susceptibility profiles, virulence potentials and genetic relatedness of Campylobacter spp. from paediatric and water samples in the North West Province, South Africa. Overall, 237 human and 20 water isolates were identified using culture and real-time polymerase chain reaction (PCR). The antibiotic susceptibility profiles were determined using the disk diffusion method. Gradient strips were used to determine the minimum inhibitory concentration of each antibiotic. Antibiotic resistance (gryA, tetO and 23S rRNA 2075G and 2074C) and virulence (cadF and ciaB) genes were also investigated using PCR. A phylogenetic tree to ascertain the clonality between water and clinical isolates was constructed using MEGA 7. Overall, 95% (water) and 64.7% (human) of the isolates were resistant to at least one antibiotic tested. The highest resistance was against clarithromycin (95%) for water and ampicillin (60.7%) for human isolates. The 23S rRNA 2075G/2074C mutation was the most expressed resistance gene. Phylogenetic reconstruction revealed eight intermixed clades within water and human Campylobacter isolates. This study suggests the possible circulation of potentially pathogenic antibiotic-resistant Campylobacter in the Northwest Province, South Africa with drinking water being a possible vector for disease transmission in this area.

scholarly journals Antimicrobial Susceptibility Profiles and Resistance Genes in Genus Aeromonas spp. Isolated from the Environment and Rainbow Trout of Two Fish Farms in France

2021 ◽  
Vol 9 (6) ◽  
pp. 1201
Author(s):  
Niki Hayatgheib ◽  
Ségolène Calvez ◽  
Catherine Fournel ◽  
Lionel Pineau ◽  
Hervé Pouliquen ◽  
...  
Keyword(s):  
Resistance Genes ◽  
Antibiotic Use ◽  
Resistant Bacteria ◽  
Aquatic Environments ◽  
Fish Farms ◽  
Antibiotic Resistant ◽  
Resistant Strains ◽  
Trout Farms ◽  
Susceptibility Profiles ◽  
The Impact

This study presents the occurrence and abundance of Aeromonas antibiotic-resistant bacteria (ARB) and genes (ARGs) isolated from water, biofilm and fish in two commercial trout farms before and one week after flumequine treatment. Wild (WT) and non-wild (NWT) strains were determined for quinolones (flumequine, oxolinic acid and enrofloxacin), oxytetracycline (OXY), florfenicol (FFN), trimethoprim-sulfamethoxazole (TMP) and colistin (COL), and pMAR (presumptive multi-resistant) strains were classified. Forty-four ARGs for the mentioned antibiotics, β-lactams and multi-resistance were quantified for 211 isolates. BlaSHV-01, mexF and tetE were the dominant ARGs. A greater occurrence and abundance of tetA2, sul3, floR1, blaSHV-01 and mexF were observed for NWT compared to WT. The occurrence of pMAR and NWT Aeromonas for quinolones, OXY, FFN, TMP, COL and ARGs depended on the Aeromonas origin, antibiotic use and the presence of upstream activities. Our results revealed the impact of a flumequine treatment on Aeromonas present on a fish farm through an increase in NWT and pMAR strains. The link between fish and their environment was shown by the detection of identical ARB and ARGs in the two types of samples. There appears to be a high risk of resistance genes developing and spreading in aquatic environments.

Prevalence, Antibiogram, and cdt Genes of Toxigenic Campylobacter jejuni in Salad Style Vegetables (Ulam) at Farms and Retail Outlets in Terengganu

2015 ◽  
Vol 78 (1) ◽  
pp. 65-71 ◽  
Author(s):  
MOHD IKHSAN KHALID ◽  
JOHN YEW HUAT TANG ◽  
NABILA HUDA BAHARUDDIN ◽  
NASIHA SHAKINA RAHMAN ◽  
NURUL FAIZZAH RAHIMI ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Multiplex Pcr ◽  
Campylobacter Jejuni ◽  
Antibiotic Susceptibility ◽  
Penicillin G ◽  
Diffusion Method ◽  
Cytolethal Distending Toxin ◽  
Disk Diffusion Method ◽  
Retail Outlets ◽  
Pcr Method

The present study was conducted to investigate the prevalence and antibiotic resistance among Campylobacter jejuni in ulam at farms and retail outlets located in Kuala Terengganu, Malaysia. A total of 526 samples (ulam, soil, and fertilizer) were investigated for the presence of C. jejuni and the gene for cytolethal distending toxin (cdt) by using a multiplex PCR method. Antibiotic susceptibility to 10 types of antibiotics was determined using the disk diffusion method for 33 C. jejuni isolates. The average prevalence of contaminated samples from farms, wet markets, and supermarkets was 35.29, 52.66, and 69.88%, respectively. The cdt gene was not detected in 24 of the 33 C. jejuni isolates, but 9 isolates harbored cdtC. Antibiotic resistance in C. jejuni isolates was highest to penicillin G (96.97% of isolates) followed by vancomycin (87.88%), ampicillin (75.76%), erythromycin (60.61%), tetracycline (9.09%), amikacin (6.06%), and norfloxacin (3.03%); none of the isolates were resistant to ciprofloxacin, enrofloxacin, and gentamicin. In this study, C. jejuni was present in ulam, and some isolates were highly resistant to some antibiotics but not to quinolones. Thus, appropriate attention and measures are required to prevent C. jejuni contamination on farms and at retail outlets.

scholarly journals Biochemical identification techniques and antibiotic susceptibility profile of lipolytic ambiental bacteria from effluents

2019 ◽  
Vol 79 (4) ◽  
pp. 555-565 ◽  
Author(s):  
A. F. G. Rave ◽  
A. V. Kuss ◽  
G. H. S. Peil ◽  
S. R. Ladeira ◽  
J. P. V. Villarreal ◽  
...  
Keyword(s):  
Antibiotic Susceptibility ◽  
Klebsiella Oxytoca ◽  
Effluent Treatment ◽  
Automated System ◽  
Resistant Bacteria ◽  
Biochemical Tests ◽  
Biochemical Identification ◽  
Identification Techniques ◽  
Susceptibility Profiles ◽  
The Impact

Abstract Different methodologies have been developed throughout the years to identify environmental microorganisms to improve bioremediation techniques, determine susceptibility profiles of bacteria in contaminated environments, and reduce the impact of microorganisms in ecosystems. Two methods of bacterial biochemical identification are compared and the susceptibility profile of bacteria, isolated from residential and industrial wastewater, is determined. Twenty-four bacteria were retrieved from the bacteria bank of the Environmental Microbiology Laboratory at the Institute of Biology (IB) of the Universidade Federal de Pelotas, Pelotas, Brazil. Bacteria were identified by conventional biochemical tests and by the VITEK ®2 automated system. Further, the susceptibility profile to antibiotics was also determined by the automated system. Six species of bacteria (Raoutella planticola, K. pneumoniae ssp. pneumoniae , Serratia marcescens, Raoutella sp., E. cloacae and Klebsiella oxytoca) were identified by conventional biochemical tests, while three species of bacteria (K. pneumoniae ssp. pneumoniae, S. marcescens and K. oxytoca ) were identified by VITEK®2 automated system. VITEK ®2 indicated agreement in 19 (79.17%) isolates and difference in five (20.83%) isolates when compared to results from conventional biochemical tests. Further, antibiotic susceptibility profile results showed that all isolates (100%) were resistant to at least one out of the 18 antibiotics tested by VITEK®2. Thus, no multi-resistant bacteria that may be used in effluent treatment systems or in bioremediation processes have been reported. Results indicate VITEK ® 2 automated system as a potential methodology in the determination of susceptibility profile and identification of environmental bacteria.

Isolation ofLegionellafrom water samples using various culture methods

1994 ◽  
Vol 76 (2) ◽  
pp. 155-162 ◽  
Author(s):  
Jaana M. Kusnetsov ◽  
Hannele R. Jousimies-Somer ◽  
Aino I. Nevalainen ◽  
P. J. Martikainen
Keyword(s):  
Water Samples ◽  
Culture Methods

scholarly journals Antipneumococcal activity of BAY 12-8039, a new quinolone, compared with activities of three other quinolones and four oral beta-lactams.

1997 ◽  
Vol 41 (12) ◽  
pp. 2786-2789 ◽  
Author(s):  
M A Visalli ◽  
M R Jacobs ◽  
P C Appelbaum
Keyword(s):  
Beta Lactam ◽  
Beta Lactams ◽  
Agar Dilution ◽  
Resistant Strains

Activities of BAY 12-8039 against 205 pneumococci were tested by agar dilution. MICs (in micrograms per milliliter) at which 50 and 90% of the isolates are inhibited (MIC50s and MIC90s, respectively) were 0.125 and 0.25 (BAY 12-8039), 2.0 and 4.0 (ciprofloxacin and ofloxacin), and 0.25 and 0.5 (sparfloxacin). Beta-lactam MIC50s and MIC90s for penicillin-susceptible, -intermediate, and -resistant strains, in that order, were 0.016 and 0.03, 0.25 and 2.0, and 2.0 and 4.0 (amoxicillin); 0.03 and 0.06, 0.25 and 4.0, and 4.0 and 8.0 (ampicillin); 0.03 and 0.06, 0.5 and 4.0, and 4.0 and 8.0 (cefuroxime); and 0.03 and 0.125, 0.25 and 2.0, and 4.0 and 8.0 (cefpodoxime). At two times their MICs after 24 h, BAY 12-8039, ciprofloxacin, ampicillin, and cefuroxime were uniformly bactericidal (99.9% killing) against 12 strains; other compounds were bactericidal at four times their MICs.

scholarly journals Isolation and identification of motile Aeromonas spp.from different sources

2011 ◽  
Vol 5 (3) ◽  
pp. 56-65
Author(s):  
Samira Y. Yousif ◽  
Rasha Abid Ali Al-Khalidi
Keyword(s):  
Aeromonas Hydrophila ◽  
Antibiotic Susceptibility ◽  
Morphological Characterization ◽  
Isolation And Identification ◽  
Aeromonas Sobria ◽  
Aeromonas Caviae ◽  
Susceptibility Tests ◽  
Different Sources

Total of 507 samples (clinical, environmental, food) were collected from different hospitals in Baghdad, water, soil, and different food stuffs. Biochemical and morphological characterization tests showed that seventeen isolates were identified as Aeromonas spp.These were farther characterized as Aeromonas hydrophila 10 isolates, Aeromonas sobria 2 isolates, Aeromonas eucrenophila 3 isolates, one isolate belongs to Aeromonas caviae and another one belongs to Aeromonas schubertii. Antibiotic susceptibility tests of all the isolates towards fifteen antibiotics agents were carried out and results showed that all isolates 100% were resistant to penicillin, ampicillin, ampiclox, 99% were resistant to lincomycin, 76.7% to cephalothin, 52.9% to cefotaxime. All isolates except one isolate of Aeromonas eucrenophila were sensitive to meropenem.

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