GDP-mannose transporter paralogues play distinct roles in polarized growth ofAspergillus nidulans

Mycologia ◽  
2010 ◽  
Vol 102 (2) ◽  
pp. 305-310 ◽  
Author(s):  
Loretta Jackson-Hayes ◽  
Terry W. Hill ◽  
Darlene M. Loprete ◽  
Barbara S. Gordon ◽  
Chassidy J. Groover ◽  
...  
Keyword(s):  
Polarized Growth ◽  
Ofaspergillus Nidulans

scholarly journals The Role of the Kinesin Motor KipA in Microtubule Organization and Polarized Growth ofAspergillus nidulans

2005 ◽  
Vol 16 (2) ◽  
pp. 497-506 ◽  
Author(s):  
Sven Konzack ◽  
Patricia E. Rischitor ◽  
Cathrin Enke ◽  
Reinhard Fischer
Keyword(s):  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Lateral Movement ◽  
Polarized Growth ◽  
Microtubule Organization ◽  
Kinesin Motor ◽  
Ofaspergillus Nidulans ◽  
Green Fluorescent ◽  
Intrinsic Motor

Polarized growth in filamentous fungi requires the integrity of the microtubule (MT) cytoskeleton. We found that growing MTs in Aspergillus nidulans merge at the center of fast growing tips and discovered that a kinesin motor protein, KipA, related to Tea2p of Schizosaccharomyces pombe, is required for this process. In a ΔkipA strain, MT plus ends reach the tip but show continuous lateral movement. Hyphae lose directionality and grow in curves, apparently due to mislocalization of the vesicle supply center (Spitzenkörper) in the apex. Green fluorescent protein (GFP)-KipA accumulates at MT plus ends, whereas a KipA rigor mutant protein, GFP-KipAG223E, coated MTs evenly. These findings suggest that KipA requires its intrinsic motor activity to reach the MT plus end. Using KipA as an MT plus-end marker, we found bidirectional organization of MTs and determined the locations of microtubule organizing centers at nuclei, in the cytoplasm, and at septa.

scholarly journals Identifying Conserved Generic Aspergillus spp. Co-Expressed Gene Modules Associated with Germination Using Cross-Platform and Cross-Species Transcriptomics

2021 ◽  
Vol 7 (4) ◽  
pp. 270
Author(s):  
Tim J. H. Baltussen ◽  
Jordy P. M. Coolen ◽  
Paul E. Verweij ◽  
Jan Dijksterhuis ◽  
Willem J. G. Melchers
Keyword(s):  
Comparative Analysis ◽  
Expression Patterns ◽  
Biological Processes ◽  
Polarized Growth ◽  
Black Module ◽  
Cross Platform ◽  
Gene Modules ◽  
Opportunistic Human Pathogen ◽  
Dependent Transport ◽  
Blue Module

Aspergillus spp. is an opportunistic human pathogen that may cause a spectrum of pulmonary diseases. In order to establish infection, inhaled conidia must germinate, whereby they break dormancy, start to swell, and initiate a highly polarized growth process. To identify critical biological processes during germination, we performed a cross-platform, cross-species comparative analysis of germinating A. fumigatus and A. niger conidia using transcriptional data from published RNA-Seq and Affymetrix studies. A consensus co-expression network analysis identified four gene modules associated with stages of germination. These modules showed numerous shared biological processes between A. niger and A. fumigatus during conidial germination. Specifically, the turquoise module was enriched with secondary metabolism, the black module was highly enriched with protein synthesis, the darkgreen module was enriched with protein fate, and the blue module was highly enriched with polarized growth. More specifically, enriched functional categories identified in the blue module were vesicle formation, vesicular transport, tubulin dependent transport, actin-dependent transport, exocytosis, and endocytosis. Genes important for these biological processes showed similar expression patterns in A. fumigatus and A. niger, therefore, they could be potential antifungal targets. Through cross-platform, cross-species comparative analysis, we were able to identify biologically meaningful modules shared by A. fumigatus and A. niger, which underscores the potential of this approach.

scholarly journals PI(3,5)P2 sows the seeds of plant growth

2012 ◽  
Vol 198 (2) ◽  
pp. 147-147
Author(s):  
Ben Short
Keyword(s):  
Plant Growth ◽  
Plant Cells ◽  
Polarized Growth

Phospholipid directs polarized growth by targeting actin-polymerizing formins to the cortex of plant cells.

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