scholarly journals BIOMARKER QUANTITATION: ANALYTICAL CONSIDERATIONS FOR LIGAND BINDING ASSAY REGRESSION CURVES AND QUALITY CONTROL SAMPLES

2012 ◽  
Vol 8 (3) ◽  
pp. 71-77 ◽  
Author(s):  
Dysinger
Keyword(s):  
Quality Control ◽  
Ligand Binding ◽  
Binding Assay ◽  
Ligand Binding Assay ◽  
Regression Curves ◽  
Biomarker Quantitation ◽  
Control Samples

scholarly journals Single-point versus five-point biochemical method for determination of estrogen and progesterone receptors

2002 ◽  
Vol 21 (1) ◽  
pp. 15-20
Author(s):  
Dragica Nikolic-Vukosavljevic ◽  
Milan Markicevic ◽  
Sladjana Todorovic
Keyword(s):  
Quality Control ◽  
Breast Carcinoma ◽  
Ligand Binding ◽  
Binding Assay ◽  
Single Point ◽  
Progesterone Receptors ◽  
Medical Decision ◽  
Ligand Binding Assay ◽  
Estrogen And Progesterone Receptors

Receptors for estrogen and progesterone are accepted by international consensus as biomarkers of breast carcinoma responsiveness to endocrine therapy. Numerous current studies are aimed at consideration of importance of "the new generation" of estrogen-regulated biomarkers in treatment of breast cancer patients. Simultaneous knowledge of all these biomarkers may help in medical decision making. However, the amount of tumor material available from breast carcinoma can make impossible determination of estrogenregulated biomarkes together with estrogen and progesterone receptors. To assess whether we could replace our current five-point ligand binding assay for measurement of estrogen and progesterone receptors with a single- point ligand binding assay, we compared simultaneous measurements in same samples of breast carcinomas by both methods. A linear regression analysis shows that single-point assay can be confidently used instead of five-point assay. In addition, there were no variations over time in estrogen and progesterone receptors phenotypes, as well as in estrogen and progesterone receptors contents determined by single-point assay. Accordingly, the results clearly demonstrate the validity of intralaboratory quality control and give a possibility for the establishment of interlaboratory quality control of single-point ligand binding assay.

TBG MEASUREMENTS BY COMPETITIVE LIGAND BINDING ASSAY (CLBA)

1975 ◽  
Vol 80 (1_Suppla) ◽  
pp. S15
Author(s):  
K. H. Rudorff ◽  
H. J. Kröll ◽  
J. Herrmann
Keyword(s):  
Ligand Binding ◽  
Binding Assay ◽  
Ligand Binding Assay ◽  
Competitive Ligand

Development and evaluation of an immunoenzymometric assay for the chicken progesterone receptor

1991 ◽  
Vol 129 (2) ◽  
pp. 189-196 ◽  
Author(s):  
M. K. Bläuer ◽  
P. J. Tuohimaa ◽  
P. J. Vilja
Keyword(s):  
Monoclonal Antibodies ◽  
Small Intestine ◽  
Horseradish Peroxidase ◽  
Progesterone Receptor ◽  
Ligand Binding ◽  
Binding Assay ◽  
Ligand Binding Assay ◽  
Coefficients Of Variation ◽  
First Time ◽  
Determination Range

ABSTRACT A specific and sensitive immunoenzymometric assay (IEMA) was developed for measuring the quantity of chicken progesterone receptor (PR) in tissue cytosol. The assay uses two monoclonal antibodies to the PR. One is used to capture the PR. The second (labelled with biotin) reacts first with the captured receptor and subsequently with avidin-labelled horseradish peroxidase to provide an enzymatic end-point. The method has a determination range from 0·3 to 60 pmol/l. Intra- and interassay coefficients of variation were 3·7% and 9·0% respectively. The assay can be performed with equal results as a rapid (3 h) or an overnight procedure. The IEMA is convenient, especially for signal measurement and the calculation of results. No ultracentrifugation of samples is needed, since the IEMA can be performed on low-speed cytosol samples. Assay results correlated well (r = 0·927) with those obtained by the conventional ligand-binding assay used in our laboratory. Similar results were obtained with the IEMA and the ligand-binding assay after exposure of cytosol samples to increased temperatures: at 20 °C the PR remained stable for the 4-h period examined, whereas at 37 °C almost complete degradation of the PR was observed in 30 min. Being more than 100 times as sensitive as the ligand-binding assay, the IEMA enabled the quantification of PR for the first time in such tissues as the bursa and small intestine even of immature animals. Journal of Endocrinology (1991) 129, 189–196

Matrix effect in ligand-binding assay: the importance of evaluating emerging technologies

Bioanalysis ◽  
2014 ◽  
Vol 6 (8) ◽  
pp. 1033-1036 ◽  
Author(s):  
Rebecca M Crisino ◽  
Linlin Luo ◽  
Brian Geist ◽  
Jad Zoghbi ◽  
Franklin Spriggs
Keyword(s):  
Ligand Binding ◽  
Matrix Effect ◽  
Emerging Technologies ◽  
Binding Assay ◽  
Ligand Binding Assay

Performance evaluation of three platforms with ultrasensitive ligand-binding assay potential

Bioanalysis ◽  
2017 ◽  
Vol 9 (12) ◽  
pp. 937-946 ◽  
Author(s):  
Kyra Juliana Cowan ◽  
Albert Geiger ◽  
Hans Hornauer ◽  
Robyn Rourick ◽  
Patricia Yanira Siguenza
Keyword(s):  
Performance Evaluation ◽  
Ligand Binding ◽  
Binding Assay ◽  
Ligand Binding Assay
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