Open-source Toolkit: Benchtop Carbon Fiber Microelectrode Array for Nerve Recording

10.3791/63099 ◽  
2021 ◽  
Author(s):  
Julianna M. Richie ◽  
Paras R. Patel ◽  
Elissa J. Welle ◽  
Tianshu Dong ◽  
Lei Chen ◽  
...  
Keyword(s):  
Carbon Fiber ◽  
Open Source ◽  
Microelectrode Array ◽  
Carbon Fiber Microelectrode

Vagus Nerve Recordings Using Carbon Fiber Microelectrode Array (CFMA) v1

2019 ◽  
Author(s):  
Ahmad Jiman ◽  
Elissa Welle ◽  
Paras Patel ◽  
David Ratze ◽  
Elizabeth Bottorff ◽  
...  
Keyword(s):  
Carbon Fiber ◽  
Vagus Nerve ◽  
Potassium Chloride ◽  
Neural Activity ◽  
Microelectrode Array ◽  
Carbon Fiber Microelectrode ◽  
Nerve Recordings

The carbon fiber microelectrode array (CFMA) has demonstrated promising results in recording single-unti neural activity. This protocol is for obtaining CFMA recordings from the cervical vagus nerve of rats in response to the application of potassium chloride (KCl) on the vagus nerve.

scholarly journals Multi-channel intraneural vagus nerve recordings with a novel high-density carbon fiber microelectrode array

2020 ◽  
Author(s):  
Ahmad A. Jiman ◽  
David C. Ratze ◽  
Elissa J. Welle ◽  
Paras R. Patel ◽  
Julianna M. Richie ◽  
...  
Keyword(s):  
Carbon Fiber ◽  
Vagus Nerve ◽  
Carbon Fibers ◽  
Action Potentials ◽  
Microelectrode Array ◽  
High Density ◽  
Autonomic Nerves ◽  
Neural Signals ◽  
Carbon Fiber Microelectrode ◽  
Regulatory Functions

AbstractAutonomic nerves convey essential neural signals that regulate vital body functions. Recording clearly distinctive physiological neural signals from autonomic nerves will help develop new treatments for restoring regulatory functions. However, this is very challenging due to the small nature of autonomic nerves and the low-amplitude signals from their small axons. We developed a multi-channel, high-density, intraneural carbon fiber microelectrode array (CFMA) with ultra-small electrodes (8-9 μm in diameter, 150-250 μm in length) for recording physiological action potentials from small autonomic nerves. In this study, we inserted CFMA with up to 16 recording carbon fibers in the cervical vagus nerve of 22 isoflurane-anesthetized rats. We recorded action potentials with peak-to-peak amplitudes of 15.1-91.7 μV and signal-to-noise ratios of 2.0-8.3 on multiple carbon fibers per experiment, determined conduction velocities of some vagal signals in the afferent (0.7-4.4 m/sec) and efferent (0.7-8.8 m/sec) directions, and monitored firing rate changes in breathing and blood glucose modulated conditions. Overall, these experiments demonstrated that CFMA is a novel interface for in-vivo intraneural action potential recordings. This work is considerable progress towards the comprehensive understanding of physiological neural signaling in vital regulatory functions controlled by autonomic nerves.

scholarly journals Multi-channel intraneural vagus nerve recordings with a novel high-density carbon fiber microelectrode array

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Ahmad A. Jiman ◽  
David C. Ratze ◽  
Elissa J. Welle ◽  
Paras R. Patel ◽  
Julianna M. Richie ◽  
...  
Keyword(s):  
Carbon Fiber ◽  
Vagus Nerve ◽  
Carbon Fibers ◽  
Action Potentials ◽  
Microelectrode Array ◽  
High Density ◽  
Autonomic Nerves ◽  
Neural Signals ◽  
Carbon Fiber Microelectrode ◽  
Regulatory Functions

Abstract Autonomic nerves convey essential neural signals that regulate vital body functions. Recording clearly distinctive physiological neural signals from autonomic nerves will help develop new treatments for restoring regulatory functions. However, this is very challenging due to the small nature of autonomic nerves and the low-amplitude signals from their small axons. We developed a multi-channel, high-density, intraneural carbon fiber microelectrode array (CFMA) with ultra-small electrodes (8–9 µm in diameter, 150–250 µm in length) for recording physiological action potentials from small autonomic nerves. In this study, we inserted CFMA with up to 16 recording carbon fibers in the cervical vagus nerve of 22 isoflurane-anesthetized rats. We recorded action potentials with peak-to-peak amplitudes of 15.1–91.7 µV and signal-to-noise ratios of 2.0–8.3 on multiple carbon fibers per experiment, determined conduction velocities of some vagal signals in the afferent (0.7–4.4 m/s) and efferent (0.7–8.8 m/s) directions, and monitored firing rate changes in breathing and blood glucose modulated conditions. Overall, these experiments demonstrated that CFMA is a novel interface for in-vivo intraneural action potential recordings. This work is considerable progress towards the comprehensive understanding of physiological neural signaling in vital regulatory functions controlled by autonomic nerves.

scholarly journals Laser Sharpening of Carbon Fiber Microelectrode Arrays for Brain Recording

2020 ◽  
Vol 8 (4) ◽  
Author(s):  
Tianshu Dong ◽  
Lei Chen ◽  
Albert Shih
Keyword(s):  
Carbon Fiber ◽  
Electrical Discharge Machining ◽  
Electrochemical Impedance ◽  
Microelectrode Arrays ◽  
Small Diameter ◽  
Input Voltage ◽  
Bath Surface ◽  
Insertion Force ◽  
Carbon Fiber Microelectrode ◽  
The Brain

Abstract Microwire microelectrode arrays (MEAs) are implanted in the brain for recording neuron activities to study the brain function. Among various microwire materials, carbon fiber stands out due to its small diameter (5–10 μm), relatively high Young's modulus, and low electrical resistance. Microwire tips in MEAs are often sharpened to reduce the insertion force and prevent the thin microwires from buckling. Currently, carbon fiber MEAs are sharpened by either torch burning, which limits the positions of wire tips to a water bath surface plane, or electrical discharge machining, which is difficult to implement to the nonelectrically conductive carbon fiber with parylene-C insulation. A laser-based carbon fiber sharpening method proposed in this study enables the fabrication of carbon fiber MEAs with sharp tips and custom lengths. Experiments were conducted to study effects of laser input voltage and transverse speed on carbon fiber tip geometry. Results of the tip sharpness and stripped length of the insulation as well as the electrochemical impedance spectroscopy measurement at 1 kHz were evaluated and analyzed. The laser input voltage and traverse speed have demonstrated to be critical for the sharp tip, short stripped length, and low electrical impedance of the carbon fiber electrode for brain recording MEAs. A carbon fiber MEA with custom electrode lengths was fabricated to validate the laser-based approach.

scholarly journals Novel carbon-fiber microelectrode batch fabrication using a 3D-printed mold and polyimide resin

The Analyst ◽  
2016 ◽  
Vol 141 (18) ◽  
pp. 5256-5260 ◽  
Author(s):  
Elefterios Trikantzopoulos ◽  
Cheng Yang ◽  
Mallikarjunarao Ganesana ◽  
Ying Wang ◽  
B. Jill Venton
Keyword(s):  
Carbon Fiber ◽  
Carbon Fiber Microelectrode ◽  
Batch Fabrication ◽  
3D Printed
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