Vagus Nerve Recordings Using Carbon Fiber Microelectrode Array (CFMA) v1

2019 ◽  
Author(s):  
Ahmad Jiman ◽  
Elissa Welle ◽  
Paras Patel ◽  
David Ratze ◽  
Elizabeth Bottorff ◽  
...  
Keyword(s):  
Carbon Fiber ◽  
Vagus Nerve ◽  
Potassium Chloride ◽  
Neural Activity ◽  
Microelectrode Array ◽  
Carbon Fiber Microelectrode ◽  
Nerve Recordings

The carbon fiber microelectrode array (CFMA) has demonstrated promising results in recording single-unti neural activity. This protocol is for obtaining CFMA recordings from the cervical vagus nerve of rats in response to the application of potassium chloride (KCl) on the vagus nerve.

scholarly journals Multi-channel intraneural vagus nerve recordings with a novel high-density carbon fiber microelectrode array

2020 ◽  
Author(s):  
Ahmad A. Jiman ◽  
David C. Ratze ◽  
Elissa J. Welle ◽  
Paras R. Patel ◽  
Julianna M. Richie ◽  
...  
Keyword(s):  
Carbon Fiber ◽  
Vagus Nerve ◽  
Carbon Fibers ◽  
Action Potentials ◽  
Microelectrode Array ◽  
High Density ◽  
Autonomic Nerves ◽  
Neural Signals ◽  
Carbon Fiber Microelectrode ◽  
Regulatory Functions

AbstractAutonomic nerves convey essential neural signals that regulate vital body functions. Recording clearly distinctive physiological neural signals from autonomic nerves will help develop new treatments for restoring regulatory functions. However, this is very challenging due to the small nature of autonomic nerves and the low-amplitude signals from their small axons. We developed a multi-channel, high-density, intraneural carbon fiber microelectrode array (CFMA) with ultra-small electrodes (8-9 μm in diameter, 150-250 μm in length) for recording physiological action potentials from small autonomic nerves. In this study, we inserted CFMA with up to 16 recording carbon fibers in the cervical vagus nerve of 22 isoflurane-anesthetized rats. We recorded action potentials with peak-to-peak amplitudes of 15.1-91.7 μV and signal-to-noise ratios of 2.0-8.3 on multiple carbon fibers per experiment, determined conduction velocities of some vagal signals in the afferent (0.7-4.4 m/sec) and efferent (0.7-8.8 m/sec) directions, and monitored firing rate changes in breathing and blood glucose modulated conditions. Overall, these experiments demonstrated that CFMA is a novel interface for in-vivo intraneural action potential recordings. This work is considerable progress towards the comprehensive understanding of physiological neural signaling in vital regulatory functions controlled by autonomic nerves.

scholarly journals Multi-channel intraneural vagus nerve recordings with a novel high-density carbon fiber microelectrode array

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Ahmad A. Jiman ◽  
David C. Ratze ◽  
Elissa J. Welle ◽  
Paras R. Patel ◽  
Julianna M. Richie ◽  
...  
Keyword(s):  
Carbon Fiber ◽  
Vagus Nerve ◽  
Carbon Fibers ◽  
Action Potentials ◽  
Microelectrode Array ◽  
High Density ◽  
Autonomic Nerves ◽  
Neural Signals ◽  
Carbon Fiber Microelectrode ◽  
Regulatory Functions

Abstract Autonomic nerves convey essential neural signals that regulate vital body functions. Recording clearly distinctive physiological neural signals from autonomic nerves will help develop new treatments for restoring regulatory functions. However, this is very challenging due to the small nature of autonomic nerves and the low-amplitude signals from their small axons. We developed a multi-channel, high-density, intraneural carbon fiber microelectrode array (CFMA) with ultra-small electrodes (8–9 µm in diameter, 150–250 µm in length) for recording physiological action potentials from small autonomic nerves. In this study, we inserted CFMA with up to 16 recording carbon fibers in the cervical vagus nerve of 22 isoflurane-anesthetized rats. We recorded action potentials with peak-to-peak amplitudes of 15.1–91.7 µV and signal-to-noise ratios of 2.0–8.3 on multiple carbon fibers per experiment, determined conduction velocities of some vagal signals in the afferent (0.7–4.4 m/s) and efferent (0.7–8.8 m/s) directions, and monitored firing rate changes in breathing and blood glucose modulated conditions. Overall, these experiments demonstrated that CFMA is a novel interface for in-vivo intraneural action potential recordings. This work is considerable progress towards the comprehensive understanding of physiological neural signaling in vital regulatory functions controlled by autonomic nerves.

Open-source Toolkit: Benchtop Carbon Fiber Microelectrode Array for Nerve Recording

10.3791/63099 ◽  
2021 ◽  
Author(s):  
Julianna M. Richie ◽  
Paras R. Patel ◽  
Elissa J. Welle ◽  
Tianshu Dong ◽  
Lei Chen ◽  
...  
Keyword(s):  
Carbon Fiber ◽  
Open Source ◽  
Microelectrode Array ◽  
Carbon Fiber Microelectrode

SPARC: Acute Glucose Regulation Recordings from the Rat Vagus Nerve Using Carbon Fiber Microelectrode Arrays

2020 ◽  
Vol 34 (S1) ◽  
pp. 1-1
Author(s):  
Ahmad A. Jiman ◽  
David C. Ratze ◽  
Elissa J. Welle ◽  
Paras R. Patel ◽  
Elizabeth C. Bottorff ◽  
...  
Keyword(s):  
Carbon Fiber ◽  
Vagus Nerve ◽  
Microelectrode Arrays ◽  
Glucose Regulation ◽  
Carbon Fiber Microelectrode

scholarly journals Short-term Effects of Vagus Nerve Stimulation on Learning and Evoked Activity in Auditory Cortex

2019 ◽  
Author(s):  
Jesyin Lai ◽  
Stephen V. David
Keyword(s):  
Auditory Cortex ◽  
Vagus Nerve ◽  
Nerve Stimulation ◽  
Neural Activity ◽  
Neural Plasticity ◽  
Neural Coding ◽  
Vagus Nerve Stimulation ◽  
Primary Auditory Cortex ◽  
Long Term Effects ◽  
Tone Frequency

ABSTRACTChronic vagus nerve stimulation (VNS) can facilitate learning of sensory and motor behaviors. VNS is believed to trigger release of neuromodulators, including norepinephrine and acetylcholine, which can mediate cortical plasticity associated with learning. Most previous work has studied effects of VNS over many days, and less is known about how acute VNS influences neural coding and behavior over the shorter term. To explore this question, we measured effects of VNS on learning of an auditory discrimination over 1-2 days. Ferrets implanted with cuff electrodes on the vagus nerve were trained by classical conditioning on a tone frequency-reward association. One tone was associated with reward while another tone, was not. The frequencies and reward associations of the tones were changed every two days, requiring learning of a new relationship. When the tones (both rewarded and non-rewarded) were paired with VNS, rates of learning increased on the first day following a change in reward association. To examine VNS effects on auditory coding, we recorded single- and multi-unit neural activity in primary auditory cortex (A1) of passively listening animals following brief periods of VNS (20 trials/session) paired with tones. Because afferent VNS induces changes in pupil size associated with fluctuations in neuromodulation, we also measured pupil during recordings. After pairing VNS with a neuron’s best-frequency (BF) tone, responses in a subpopulation of neurons were reduced. Pairing with an off-BF tone or performing VNS during the inter-trial interval had no effect on responses. We separated the change in A1 activity into two components, one that could be predicted by fluctuations in pupil and one that persisted after VNS and was not accounted for by pupil. The BF-specific reduction in neural responses remained, even after regressing out changes that could be explained by pupil. In addition, the size of VNS-mediated changes in pupil predicted the magnitude of persistent changes in the neural response. This interaction suggests that changes in neuromodulation associated with arousal gate the long-term effects of VNS on neural activity. Taken together, these results support a role for VNS in auditory learning and help establish VNS as a tool to facilitate neural plasticity.

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