Live Imaging of the Mitochondrial Glutathione Redox State in Primary Neurons using a Ratiometric Indicator

10.3791/63073 ◽  
2021 ◽  
Author(s):  
Athanasios Katsalifis ◽  
Angela Maria Casaril ◽  
Constanze Depp ◽  
Carlos Bas-Orth
Keyword(s):  
Redox State ◽  
Live Imaging ◽  
Primary Neurons ◽  
Mitochondrial Glutathione ◽  
Glutathione Redox

Acute nutrient regulation of the mitochondrial glutathione redox state in pancreatic β-cells

2014 ◽  
Vol 460 (3) ◽  
pp. 411-423 ◽  
Author(s):  
Hilton K. Takahashi ◽  
Laila R. B. Santos ◽  
Letícia P. Roma ◽  
Jessica Duprez ◽  
Christophe Broca ◽  
...  
Keyword(s):  
Redox Potential ◽  
Redox State ◽  
Β Cells ◽  
Pancreatic Β Cells ◽  
Nutrient Regulation ◽  
Mitochondrial Glutathione ◽  
Glutathione Redox

Nutrient stimulation acutely reduces mitochondrial, but not cytosolic/nuclear, glutathione redox potential (EGSH) in insulin-secreting β-cells under control conditions. These changes are negatively correlated with NAD(P)H autofluorescence, but independent from changes in intracellular Ca2+ or mitochondrial pH.

scholarly journals Optogenetic Monitoring of the Glutathione Redox State in Engineered Human Myocardium

2019 ◽  
Vol 10 ◽  
Author(s):  
Irina Trautsch ◽  
Eriona Heta ◽  
Poh Loong Soong ◽  
Elif Levent ◽  
Viacheslav O. Nikolaev ◽  
...  
Keyword(s):  
Redox State ◽  
Human Myocardium ◽  
Glutathione Redox

scholarly journals Effect of glutathione redox state on Leydig cell susceptibility to acute oxidative stress

2010 ◽  
Vol 323 (2) ◽  
pp. 147-154 ◽  
Author(s):  
Haolin Chen ◽  
Liang Zhou ◽  
Chieh-Yin Lin ◽  
Matthew C. Beattie ◽  
June Liu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Leydig Cell ◽  
Redox State ◽  
Glutathione Redox

scholarly journals The Effectiveness of Glutathione Redox Status as a Possible Tumor Marker in Colorectal Cancer

2021 ◽  
Vol 22 (12) ◽  
pp. 6183
Author(s):  
Delia Acevedo-León ◽  
Lidia Monzó-Beltrán ◽  
Segundo Ángel Gómez-Abril ◽  
Nuria Estañ-Capell ◽  
Natalia Camarasa-Lillo ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Redox State ◽  
Reduced Glutathione ◽  
Redox Status ◽  
Oxidation Products ◽  
Interventional Study ◽  
Thiol Redox State ◽  
Thiol Redox ◽  
Glutathione Redox

The role of oxidative stress (OS) in cancer is a matter of great interest due to the implication of reactive oxygen species (ROS) and their oxidation products in the initiation of tumorigenesis, its progression, and metastatic dissemination. Great efforts have been made to identify the mechanisms of ROS-induced carcinogenesis; however, the validation of OS byproducts as potential tumor markers (TMs) remains to be established. This interventional study included a total of 80 colorectal cancer (CRC) patients and 60 controls. By measuring reduced glutathione (GSH), its oxidized form (GSSG), and the glutathione redox state in terms of the GSSG/GSH ratio in the serum of CRC patients, we identified significant changes as compared to healthy subjects. These findings are compatible with the effectiveness of glutathione as a TM. The thiol redox state showed a significant increase towards oxidation in the CRC group and correlated significantly with both the tumor state and the clinical evolution. The sensitivity and specificity of serum glutathione levels are far above those of the classical TMs CEA and CA19.9. We conclude that the GSSG/GSH ratio is a simple assay which could be validated as a novel clinical TM for the diagnosis and monitoring of CRC.

scholarly journals Resolving diurnal dynamics of the chloroplastic glutathione redox state in Arabidopsis reveals its photosynthetically-derived oxidation

2021 ◽  
Author(s):  
Zechariah Haber ◽  
Nardy Lampl ◽  
Andreas J Meyer ◽  
Einat Zelinger ◽  
Matanel Hipsch ◽  
...  
Keyword(s):  
Redox State ◽  
Fluorescent Protein ◽  
Operating Efficiency ◽  
High Temporal Resolution ◽  
Fluctuating Light ◽  
Gsh Metabolism ◽  
Glutathione Cycle ◽  
Green Fluorescent ◽  
Glutathione Redox

Abstract Plants are subjected to fluctuations in light intensity, and this causes unbalanced photosynthetic electron fluxes and overproduction of reactive oxygen species (ROS). Electrons needed for ROS detoxification are drawn, at least partially, from the cellular glutathione (GSH) pool via the ascorbate-glutathione cycle. Here, we explore the dynamics of the chloroplastic glutathione redox potential (chl-EGSH) using high-temporal-resolution monitoring of Arabidopsis (Arabidopsis thaliana) lines expressing the reduction-oxidation sensitive green fluorescent protein 2 (roGFP2in chloroplasts. This was carried out over several days, under dynamic environmental conditions and in correlation with PSII operating efficiency. Peaks in chl-EGSH oxidation during dark-to-light and light-to-dark transitions were observed. Increasing light intensities triggered a binary oxidation response, with a threshold around the light saturating point, suggesting two regulated oxidative states of the chl-EGSH. These patterns were not affected in npq1 plants, which are impaired in nonphotochemical quenching. Oscillations between the two oxidation states were observed under fluctuating light in WT and npq1 plants, but not in pgr5 plants, suggesting a role for PSI photoinhibition in regulating the chl-EGSH dynamics. Remarkably, pgr5 plants showed an increase in chl-EGSH oxidation during the nights following light stresses, linking daytime photoinhibition and nighttime GSH metabolism. This work provides a systematic view of the dynamics of the in vivo chloroplastic glutathione redox state during varying light conditions.

Does Leukotriene Affect Intracellular Glutathione Redox State in Cultured Human Airway Epithelial Cells?

2008 ◽  
Vol 10 (4) ◽  
pp. 821-828 ◽  
Author(s):  
Junying Wang ◽  
Hiroyuki Mochizuki ◽  
Makoto Todokoro ◽  
Hirokazu Arakawa ◽  
Akihiro Morikawa
Keyword(s):  
Epithelial Cells ◽  
Redox State ◽  
Airway Epithelial Cells ◽  
Airway Epithelial ◽  
Human Airway ◽  
Intracellular Glutathione ◽  
Glutathione Redox ◽  
Human Airway Epithelial Cells

Regulation of the Intracellular Glutathione Redox State in Asthma

2019 ◽  
Author(s):  
T. Nagasaki ◽  
Y. Deng ◽  
J.B. Trudeau ◽  
Y. Minami ◽  
N. Bonfiglio ◽  
...  
Keyword(s):  
Redox State ◽  
Intracellular Glutathione ◽  
Glutathione Redox

scholarly journals Grape antioxidant dietary fibre reduced apoptosis and induced a pro-reducing shift in the glutathione redox state of the rat proximal colonic mucosa

2009 ◽  
Vol 103 (8) ◽  
pp. 1110-1117 ◽  
Author(s):  
María Elvira López-Oliva ◽  
Angel Agis-Torres ◽  
Isabel Goñi ◽  
Emilia Muñoz-Martínez
Keyword(s):  
Dietary Fibre ◽  
Redox State ◽  
Colonic Mucosa ◽  
Control Diet ◽  
Natural Antioxidants ◽  
Redox System ◽  
Diet Group ◽  
Antioxidant Enzyme Activities ◽  
Male Wistar Rats ◽  
Glutathione Redox

Grape antioxidant dietary fibre (GADF) is a grape product rich in dietary fibre and natural antioxidants. We showed previously that the GADF intake induced an epithelial hypoplasia in the rat colonic mucosa. In the present study, we propose that the antioxidant effect of GADF could modulate mucosal apoptosis via modulation of the cellular redox environment. Male Wistar rats (n20) were fed with diets containing either cellulose (control diet group) or GADF (GADF diet group) as fibre for 4 weeks. The GSH:GSSG ratio, the redox state of the GSSG/2GSH couple (Ehc), the mitochondrial and/or cytosolic antioxidant enzyme activities (superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx)), lipid peroxidation (LPO) and apoptosis were evaluated. GADF enhanced the cytosolic GSH:GSSG ratio, shifting the redox potential (Ehc) to a more pro-reducing status. Decreased Cu,ZnSOD:CAT, Cu,ZnSOD:GPx and MnSOD:GPx ratios could indicate an enhanced capacity for reducing H2O2, contributing to decreased cytosolic LPO. Reduced apoptosis in GADF-treated mucosa was inversely related to MnSOD activity. Furthermore, apoptosis increased directly as GSSG content increased. These results suggest that the reduction in apoptosis associated with GADF intake may be due to a modulation of the glutathione redox system and endogenous antioxidant enzymes.

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