scholarly journals Characterization of Neuromuscular Junctions in Mice by Combined Confocal and Super-Resolution Microscopy

10.3791/63032 ◽  
2021 ◽  
Author(s):  
Martina Marinello ◽  
Jérémie Cosette ◽  
Caroline Bogni ◽  
Jérôme Denard ◽  
Daniel Stockholm ◽  
...  
Keyword(s):  
Neuromuscular Junctions ◽  
Super Resolution ◽  
Super Resolution Microscopy

scholarly journals Characterization of Plasma Membrane Ceramides by Super-Resolution Microscopy

2017 ◽  
Vol 56 (22) ◽  
pp. 6131-6135 ◽  
Author(s):  
Anne Burgert ◽  
Jan Schlegel ◽  
Jérôme Bécam ◽  
Sören Doose ◽  
Erhard Bieberich ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Super Resolution ◽  
Super Resolution Microscopy

scholarly journals Can Super-Resolution Microscopy Become a Standard Characterization Technique for Material Chemistry?

2021 ◽  
Author(s):  
Shikha Dhiman ◽  
Teodora Andrian ◽  
Beatriz Santiago ◽  
Marrit Tholen ◽  
Yuyang Wang ◽  
...  
Keyword(s):  
Super Resolution ◽  
Analytical Techniques ◽  
Material Chemistry ◽  
Characterization Technique ◽  
Super Resolution Microscopy ◽  
Synthesized Materials

The characterization of newly synthesized materials is a cornerstone of all chemistry and nanotechnology laboratories. For this purpose, a wide array of analytical techniques have been standardized and are used...

scholarly journals Experimental characterization of 3D localization techniques for particle-tracking and super-resolution microscopy

2009 ◽  
Vol 17 (10) ◽  
pp. 8264 ◽  
Author(s):  
Michael J. Mlodzianoski ◽  
Manuel F. Juette ◽  
Glen L. Beane ◽  
Joerg Bewersdorf
Keyword(s):  
Particle Tracking ◽  
Super Resolution ◽  
Experimental Characterization ◽  
3D Localization ◽  
Super Resolution Microscopy

scholarly journals Characterization of Ire1 Interactions and Dynamics with Quantitative Super-Resolution Microscopy

2017 ◽  
Vol 112 (3) ◽  
pp. 148a
Author(s):  
Elizabeth M. Smith ◽  
Ragnar Stefansson ◽  
Maria Paz Ramirez Lopez ◽  
Elias M. Puchner
Keyword(s):  
Super Resolution ◽  
Super Resolution Microscopy

scholarly journals A new probe for super-resolution imaging of membranes elucidates trafficking pathways

2014 ◽  
Vol 205 (4) ◽  
pp. 591-606 ◽  
Author(s):  
Natalia H. Revelo ◽  
Dirk Kamin ◽  
Sven Truckenbrodt ◽  
Aaron B. Wong ◽  
Kirsten Reuter-Jessen ◽  
...  
Keyword(s):  
Synaptic Vesicles ◽  
Cultured Cells ◽  
Neuromuscular Junctions ◽  
Organ Of Corti ◽  
Super Resolution ◽  
Molecular Composition ◽  
Cultured Neurons ◽  
Vesicle Recycling ◽  
Resolution Imaging ◽  
Super Resolution Microscopy

The molecular composition of the organelles involved in membrane recycling is difficult to establish as a result of the absence of suitable labeling tools. We introduce in this paper a novel probe, named membrane-binding fluorophore-cysteine-lysine-palmitoyl group (mCLING), which labels the plasma membrane and is taken up during endocytosis. It remains attached to membranes after fixation and permeabilization and can therefore be used in combination with immunostaining and super-resolution microscopy. We applied mCLING to mammalian-cultured cells, yeast, bacteria, primary cultured neurons, Drosophila melanogaster larval neuromuscular junctions, and mammalian tissue. mCLING enabled us to study the molecular composition of different trafficking organelles. We used it to address several questions related to synaptic vesicle recycling in the auditory inner hair cells from the organ of Corti and to investigate molecular differences between synaptic vesicles that recycle actively or spontaneously in cultured neurons. We conclude that mCLING enables the investigation of trafficking membranes in a broad range of preparations.

scholarly journals Towards a Quantitative Single Particle Characterization by Super Resolution Microscopy: From Virus Structures to Antivirals Design

2021 ◽  
Vol 9 ◽  
Author(s):  
Maria Arista-Romero ◽  
Silvia Pujals ◽  
Lorenzo Albertazzi
Keyword(s):  
Viral Infection ◽  
Fluorescent Dyes ◽  
Super Resolution ◽  
Particle Characterization ◽  
Emerging Viruses ◽  
Potential Threat ◽  
The Right ◽  
Super Resolution Microscopy ◽  
Selection Of

In the last year the COVID19 pandemic clearly illustrated the potential threat that viruses pose to our society. The characterization of viral structures and the identification of key proteins involved in each step of the cycle of infection are crucial to develop treatments. However, the small size of viruses, invisible under conventional fluorescence microscopy, make it difficult to study the organization of protein clusters within the viral particle. The applications of super-resolution microscopy have skyrocketed in the last years, converting this group into one of the leading techniques to characterize viruses and study the viral infection in cells, breaking the diffraction limit by achieving resolutions up to 10 nm using conventional probes such as fluorescent dyes and proteins. There are several super-resolution methods available and the selection of the right one it is crucial to study in detail all the steps involved in the viral infection, quantifying and creating models of infection for relevant viruses such as HIV-1, Influenza, herpesvirus or SARS-CoV-1. Here we review the use of super-resolution microscopy (SRM) to study all steps involved in the viral infection and antiviral design. In light of the threat of new viruses, these studies could inspire future assays to unveil the viral mechanism of emerging viruses and further develop successful antivirals against them.

scholarly journals Active diffusion and advection in theDrosophilaooplasm result from the interplay of the actin and microtubule cytoskeletons

2017 ◽  
Author(s):  
Maik Drechsler ◽  
Fabio Giavazzi ◽  
Roberto Cerbino ◽  
Isabel M. Palacios
Keyword(s):  
Actin Cytoskeleton ◽  
Super Resolution ◽  
Noisy Environment ◽  
Directed Motion ◽  
Contrast Mechanisms ◽  
Active Processes ◽  
Super Resolution Microscopy ◽  
Random Diffusion ◽  
Dynamic Link

ABSTRACTTransport in cells occurs via a delicate interplay of passive and active processes, including diffusion, directed transport and advection. Despite progresses in super-resolution microscopy, discriminating and quantifying these processes is a challenge, requiring tracking of rapidly moving, sub-diffraction objects in a crowded, noisy environment. Here we use Differential Dynamic Microscopy with different contrast mechanisms to provide a thorough characterization of the dynamics in theDrosophilaoocyte. We study the movement of vesicles and the elusive motion of a cytoplasmic F-actin mesh, a known regulator of cytoplasmic flows. We find that cytoplasmic motility constitutes a combination of directed motion and random diffusion. While advection is mainly attributed to microtubules, we find that active diffusion is driven by the actin cytoskeleton, although it is also enhanced by the flow. We also find that an important dynamic link exists between vesicles and cytoplasmic F-actin motion, as recently suggested in mouse oocytes.

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